Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

Wu, Xuebing, Scott, David A, Kriz, Andrea J, Chiu, Anthony C, Hsu, Patrick D, Dadon, Daniel B, Cheng, Albert W, Trevino, Alexandro E, Konermann, Silvana, Chen, Sidi, Jaenisch, Rudolf, Zhang, Feng, Sharp, Phillip A·2014·Nature Biotechnology

DOI 10.1038/nbt.2889

Cited by (944)

Items 301–400 of 944

Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing
Jinjing Li, Shunyan Hong, Wan‐Jin Chen, Erwei Zuo, Hui Yang · 2019 · Journal of genetics and genomics/Journal of Genetics and Genomics
Error-corrected next generation sequencing – Promises and challenges for genotoxicity and cancer risk assessment
Francesco Marchetti, Renato Cardoso, Connie L. Chen, George R. Douglas, Joanne M. Elloway, Patricia A. Escobar, Tod A. Harper, Robert H. Heflich, Darren Kidd, Anthony M. Lynch et al. · 2023 · Mutation Research/Reviews in Mutation Research
CRISPR–Cas9-mediated genome editing and guide RNA design
Michael V. Wiles, Wenning Qin, Albert W. Cheng, Haoyi Wang · 2015 · Mammalian Genome
dCas9-mediated Nanoelectrokinetic Direct Detection of Target Gene for Liquid Biopsy
Hyomin Lee, Jihye Choi, Euihwan Jeong, Seongho Baek, Hee Chan Kim, Jong‐Hee Chae, Youngil Koh, Sang Woo Seo, Jin‐Soo Kim, Sung Jae Kim · 2018 · Nano Letters
Reversible Disruption of Specific Transcription Factor-DNA Interactions Using CRISPR/Cas9
S. Ali Shariati, Antonia A. Dominguez, Shicong Xie, Marius Wernig, Lei S. Qi, Jan Marcel Skotheim · 2019 · Molecular Cell
Gene Editing and Genotoxicity: Targeting the Off-Targets
Georges Blattner, Alessia Cavazza, Adrian James Thrasher, Giandomenico Turchiano · 2020 · Frontiers in Genome Editing
Selective TnsC recruitment enhances the fidelity of RNA-guided transposition
Florian T. Hoffmann, Minjoo Kim, Leslie Y. Beh, Jing Wang, Phuc Leo H. Vo, Diego R. Gelsinger, Jerrin Thomas George, Christopher Acree, Jason T. Mohabir, Israel S. Fernández et al. · 2022 · Nature
Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
Grace N. Hibshman, Jack P. K. Bravo, Matthew M. Hooper, Tyler L. Dangerfield, Hongshan Zhang, Ilya J. Finkelstein, Kenneth Allen Johnson, David Ward Taylor · 2024 · Nature Communications
Tracking and transforming neocortical progenitors by CRISPR/Cas9 gene targeting and PiggyBac transposase lineage labeling
Fuyi Chen, Joel A. Rosiene, Alicia Che, Albert J. Becker, Joseph J. LoTurco · 2015 · Development
Genome Editing with CRISPR-Cas9: Can It Get Any Better?
Maximilian Haeussler, Jean‐Paul Concordet · 2016 · Journal of genetics and genomics/Journal of Genetics and Genomics
T7 Polymerase Expression of Guide RNAs in vivo Allows Exportable CRISPR-Cas9 Editing in Multiple Yeast Hosts
Nicholas J. Morse, James M. Wagner, Kevin B. Reed, Madan R. Gopal, Lars H. Lauffer, Hal S. Alper · 2018 · ACS Synthetic Biology
Transposon-encoded nucleases use guide RNAs to promote their selfish spread
Meers, Chance, Le, Hoang C., Pesari, Sanjana R., Hoffmann, Florian T., Walker, Matt W. G., Gezelle, Jeanine, Tang, Stephen, Sternberg, Samuel H. · 2023 · Nature
A screening method to identify efficient sgRNAs in Arabidopsis, used in conjunction with cell-specific lignin reduction
Yan Liang, Aymerick Eudes, Sasha Yogiswara, Beibei Jing, Veronica Teixeira Benites, Reo Yamanaka, Clarabelle Cheng-Yue, Edward E. K. Baidoo, Jenny C. Mortimer, Henrik Vibe Scheller et al. · 2019 · Biotechnology for Biofuels
Optimizing Systems for Cas9 Expression in Toxoplasma gondii
Benedikt M. Markus, George W. Bell, Hernán Lorenzi, Sebastian Lourido · 2019 · mSphere
CRISPR system: Discovery, development and off-target detection
Chen, Shengmiao, Yao, Yufeng, Zhang, Yanchun, Fan, Gaofeng · 2020 · Cellular Signalling
Quantification of Cas9 binding and cleavage across diverse guide sequences maps landscapes of target engagement
Evan A. Boyle, Winston R. Becker, Hua Bai, Janice S. Chen, Jennifer A. Doudna, William James Greenleaf · 2021 · Science Advances
Muscle growth in targeted knockout common carp (Cyprinus carpio) mstn gene with low off-target effects
Neetu Shahi, Sumanta Kumar Mallik, Debajit Sarma · 2021 · Aquaculture
CRISPR-Cas9 for medical genetic screens: applications and future perspectives
Hui-Ying Xue, Li-Juan Ji, Ai-Mei Gao, Ping Liu, Jing-Dong He, Xiaojie Lu · 2015 · Journal of Medical Genetics
Impact of CRISPR-Cas9-Based Genome Engineering in Farm Animals
Parul Singh, Syed Azmal Ali · 2021 · Veterinary Sciences
Chemical Biology Approaches to Genome Editing: Understanding, Controlling, and Delivering Programmable Nucleases
Johnny H. Hu, Kevin M Davis, David R. Liu · 2016 · Cell chemical biology
Efficient genome editing in hematopoietic stem cells with helper-dependent Ad5/35 vectors expressing site-specific endonucleases under microRNA regulation
Kamola Saydaminova, Xun Ye, Hongjie Wang, Maximilian Richter, Martin Ho, Hongzhuan Chen, Ning Xu, Jin‐Soo Kim, Eirini P. Papapetrou, Michael C. Holmes et al. · 2015 · Molecular Therapy — Methods & Clinical Development
A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular Research
Joseph M. Miano, Qiuyu Martin Zhu, Charles J. Lowenstein · 2016 · Arteriosclerosis Thrombosis and Vascular Biology
Determination of local chromatin interactions using a combined CRISPR and peroxidase APEX2 system
Wenqing Qiu, Zhijiao Xu, Min Zhang, Dandan Zhang, Hui Fan, Taotao Li, Qianfeng Wang, Peiru Liu, Zaihua Zhu, Duo Du et al. · 2019 · Nucleic Acids Research
Site-Specific and Enzymatic Cross-Linking of sgRNA Enables Wavelength-Selectable Photoactivated Control of CRISPR Gene Editing
Dongyang Zhang, Luping Liu, Shuaijiang Jin, Ember M. Tota, Zijie Li, Xijun Piao, Xuan Zhang, Xiang‐Dong Fu, Neal K. Devaraj · 2022 · Journal of the American Chemical Society
CRISPR/Cas9: From Genome Engineering to Cancer Drug Discovery
Ji Luo · 2016 · Trends in cancer
Design principles for nuclease-deficient CRISPR-based transcriptional regulators
Michael Krogh Jensen · 2018 · FEMS Yeast Research
Synthesis of an arrayed sgRNA library targeting the human genome
Tobias Schmidt, Jonathan Leo Schmid-Burgk, Veit Hornung · 2015 · Scientific Reports
PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing
Abba Malina, Christopher J. F. Cameron, Francis Robert, Mathieu Blanchette, Josée Dostie, Jerry Pelletier · 2015 · Nature Communications
Cell Reprogramming With CRISPR/Cas9 Based Transcriptional Regulation Systems
К. М. Шакирова, Viktoriia Y. Ovchinnikova, Erdem Bairovich Dashinimaev · 2020 · Frontiers in Bioengineering and Biotechnology
Cloning-free CRISPR
Mandana Arbab, Sharanya Srinivasan, Tatsunori Hashimoto, Niels Geijsen, Richard I. Sherwood · 2015 · Stem Cell Reports
Effects of CRISPR/Cas9 dosage on TICAM1 and RBL gene mutation rate, embryonic development, hatchability and fry survival in channel catfish
Ahmed Elaswad, Karim M. Khalil, Zhi Ye, Zhanjiang Liu, Shikai Liu, Eric Peatman, Ramjie Odin, Khoi Minh Vo, David Drescher, Kamal Gosh et al. · 2018 · Scientific Reports
Chemical engineering of CRISPR–Cas systems for therapeutic application
Halle M. Barber, Adrian A. Pater, Keith T. Gagnon, Masad José Damha, Daniel O’Reilly · 2024 · Nature Reviews Drug Discovery
Cutting Edge Genetics: CRISPR/Cas9 Editing of Plant Genomes
Cara L. Soyars, Brenda A. Peterson, Christian A. Burr, Zachary L. Nimchuk · 2018 · Plant and Cell Physiology
Systems Metabolic Engineering of Escherichia coli
Kyeong Rok Choi, Jae Ho Shin, Jae Sung Cho, Dongsoo Yang, Sang Yup Lee · 2016 · EcoSal Plus
Targeting cancer epigenetics with CRISPR-dCAS9: Principles and prospects
Mohammad Mijanur Rahman, Trygve O. Tollefsbol · 2020 · Methods
Human sensory neurons derived from pluripotent stem cells for disease modelling and personalized medicine
Angelika Lampert, David Bennett, Lucy McDermott, Anika Neureiter, Esther Eberhardt, Beate Winner, Martin Zenke · 2020 · Neurobiology of Pain
Genome edited wheat- current advances for the second green revolution
Muhammad Jawad Akbar Awan, Komal Pervaiz, Awais Rasheed, Imran Ul Amin, Nasir Ahmad Saeed, Kanwarpal S. Dhugga, Shahid Mansoor · 2022 · Biotechnology Advances
CRISPR-Cas9: a promising genetic engineering approach in cancer research
Zubair Ahmed Ratan, Young–Jin Son, Mohammad Faisal Haidere, Bhuiyan Mohammad Mahtab Uddin, Md Abdullah Yusuf, Sojib Bin Zaman, Jong‐Hoon Kim, Laila Anjuman Banu, Jae Youl Cho · 2018 · Therapeutic Advances in Medical Oncology
Sequence specificity in DNA binding is mainly governed by association
Emil Marklund, Guanzhong Mao, Jinwen Yuan, Spartak Zikrin, Eldar Abdurakhmanov, Sebastian Deindl, Johan Elf · 2022 · Science
Extracellular vesicles associated microRNAs: Their biology and clinical significance as biomarkers in gastrointestinal cancers
Yuan Li, Silei Sui, Ajay Goel · 2024 · Seminars in Cancer Biology
Chimeric Guides Probe and Enhance Cas9 Biochemical Activity
Zachary J. Kartje, Christopher L. Barkau, Kushal J. Rohilla, Eman A. Ageely, Keith T. Gagnon · 2018 · Biochemistry
The Chromatin Structure of CRISPR-Cas9 Target DNA Controls the Balance between Mutagenic and Homology-Directed Gene-Editing Events
Josephine M. Janssen, Xiaoyu Chen, Jin Liu, Manuel A.F.V. Gonçalves · 2019 · Molecular Therapy — Nucleic Acids
Impact of CRISPR interference on strain development in biotechnology
Kerstin Schultenkämper, Luciana Fernandes Brito, Volker F. Wendisch · 2020 · Biotechnology and Applied Biochemistry
The Power of CRISPR-Cas9-Induced Genome Editing to Speed Up Plant Breeding
Hieu X. Cao, Wenqin Wang, Lê Thị Thu Hiền, Giang T. H. Vu · 2016 · International Journal of Genomics
CRISPR therapeutic tools for complex genetic disorders and cancer (Review)
Stella Baliou, Maria Adamaki, Anthony M. Kyriakopoulos, Demetrios A. Spandidos, Mihalis I. Panayiotidis, Ioannis Christodoulou, Vassilis C. Zoumpourlis · 2018 · International Journal of Oncology
Deciphering essential cistromes using genome-wide CRISPR screens
Teng Fei, Wei Li, Jingyu Peng, Tengfei Xiao, Chen-Hao Chen, Alexander Wu, Jialiang Huang, Chongzhi Zang, X. Shirley Liu, Myles A Brown · 2019 · Proceedings of the National Academy of Sciences
Genome editing enables reverse genetics of multicellular development in the choanoflagellate Salpingoeca rosetta
David Scott Booth, Nicole King · 2020 · eLife
Inhibition of CRISPR-Cas12a DNA targeting by nucleosomes and chromatin
Isabel Strohkendl, Fatema A. Saifuddin, Bryan A. Gibson, Michael K. Rosen, Rick Russell, Ilya J. Finkelstein · 2021 · Science Advances
Induction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9
Junfeng Jiang, Li Zhang, Xingliang Zhou, Xi Chen, Guanyi Huang, Fengsheng Li, Ruizhe Wang, Nancy L. Wu, Youzhen Yan, Chang Tong et al. · 2016 · Scientific Reports
Rapid Generation of Human Genetic Loss-of-Function iPSC Lines by Simultaneous Reprogramming and Gene Editing
Andrew M. Tidball, Louis T. Dang, Trevor W. Glenn, Emma G. Kilbane, Daniel Klarr, Joshua L. Margolis, Michael D. Uhler, Jack M. Parent · 2017 · Stem Cell Reports
Insights into maize genome editing via CRISPR/Cas9
Astha Agarwal, Pranjal Yadava, Krishan Kumar, Ishwar Singh, Tanushri Kaul, A. Pattanayak, Pawan Kumar Agrawal · 2018 · Physiology and Molecular Biology of Plants
SARS-CoV-2 in wastewater: From detection to evaluation
Danwei Zhang, Solco S. Faye Duran, Wei Yang Lim, Chee Kiang Ivan Tan, Wun Chet Davy Cheong, Ady Suwardi, Xian Jun Loh · 2022 · Materials Today Advances
Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites
Roger S. Zou, Alberto Marín-González, Yang Liu, Hans B. Liu, Leo L. Shen, Rachel K. Dveirin, Jay X. J. Luo, Reza Kalhor, Taekjip Ha · 2022 · Nature Cell Biology
Benchmarking deep learning methods for predicting CRISPR/Cas9 sgRNA on- and off-target activities
Zhang, Guishan, Luo, Ye, Dai, Xianhua, Dai, Zhiming · 2023 · Briefings in Bioinformatics
Extracellular Vesicles in Therapeutics: A Comprehensive Review on Applications, Challenges, and Clinical Progress
Jiyoung Goo, Yeji Lee, Jeongmin Lee, In‐San Kim, Cherlhyun Jeong · 2024 · Pharmaceutics
Genome engineering in ophthalmology: Application of CRISPR/Cas to the treatment of eye disease
Sandy S. C. Hung, Tristan McCaughey, Olivia G. Swann, Alice Pébay, Alex William Hewitt · 2016 · Progress in Retinal and Eye Research
Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells
Sanne Hindriksen, Arne J. Bramer, My Anh Truong, Martijn J.M. Vromans, Jasmin B. Post, Ingrid Verlaan-Klink, Hugo J.G. Snippert, Susanne M. A. Lens, Michael A. Hadders · 2017 · PLoS ONE
Highly efficient gene inactivation by adenoviral CRISPR/Cas9 in human primary cells
Voets, Olaf, Tielen, Frans, Elstak, Edo, Benschop, Julian, Grimbergen, Max, Stallen, Jan, Janssen, Richard, van Marle, Andre, Essrich, Christian · 2017 · PLOS ONE
CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering
Bilal Ahmad Lone, Shibendra Kumar Lal Karna, Faiz Ahmad, Nerina Shahi, Yuba Raj Pokharel · 2018 · Genetics Research International
Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression
Ami M. Kabadi, Charles A. Gersbach · 2014 · Methods
The CRISPR/Cas9 system targeting EGFR exon 17 abrogates NF-κB activation via epigenetic modulation of UBXN1 in EGFRwt/vIII glioma cells
Kai Huang, Chao Yang, Qixue Wang, Yan-sheng Li, Chuan Fang, Yanli Tan, Jian-wei Wei, Yunfei Wang, Xin Li, Junhu Zhou et al. · 2016 · Cancer Letters
Detecting and typing target DNA with a novel CRISPR-typing PCR (ctPCR) technique
Beibei Zhang, Qiang Xia, Qiao Wang, Xinyi Xia, Jinke Wang · 2018 · Analytical Biochemistry
CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics
Mark J. Osborn, Akshay Bhardwaj, Samuel P. Bingea, Friederike Knipping, Colby J. Feser, Christopher J. Lees, Daniel P. Collins, Clifford J. Steer, Bruce R. Blazar, Jakub Tolar · 2021 · Bioengineering
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Debajit Das, Dhanawantari L. Singha, Ricky Raj Paswan, Naimisha Chowdhury, Monica Sharma, Palakolanu Sudhakar Reddy, Channakeshavaiah Chikkaputtaiah · 2022 · Planta
A modular open platform for systematic functional studies under physiological conditions
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Conformational regulation of CRISPR-associated nucleases
Ryan N. Jackson, Paul B. G. van Erp, Samuel H. Sternberg, Blake Wiedenheft · 2017 · Current Opinion in Microbiology
The Chromatin Structure Differentially Impacts High-Specificity CRISPR-Cas9 Nuclease Strategies
Xiaoyu Chen, Jin Liu, Josephine M. Janssen, Manuel A.F.V. Gonçalves · 2017 · Molecular Therapy — Nucleic Acids
A benchmark of computational CRISPR-Cas9 guide design methods
Jacob Bradford, Dimitri Perrin · 2019 · PLoS Computational Biology
Technologies and Computational Analysis Strategies for CRISPR Applications
Kendell Clement, Jonathan Y. Hsu, Matthew C. Canver, J. Keith Joung, Luca Pinello · 2020 · Molecular Cell
PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics
Sundaram Acharya, Asgar Hussain Ansari, Prosad Kumar Das, Seiichi Hirano, Meghali Aich, Riya Rauthan, Sudipta Mahato, Savitri Maddileti, Sajal Sarkar, Manoj Kumar et al. · 2024 · Nature Communications
CRISPR‐driven diagnostics: Molecular mechanisms, clinical efficacy and translational challenges
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Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries
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Tuning CRISPR-Cas9 Gene Drives in Saccharomyces cerevisiae
Emily Roggenkamp, Rachael M. Giersch, Madison N. Schrock, Emily Turnquist, Megan Halloran, Gregory C. Finnigan · 2018 · G3 Genes Genomes Genetics
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Behnom Farboud, Erin Jarvis, Theodore L. Roth, Jiyung J. Shin, Jacob E. Corn, Alexander Marson, Barbara J Meyer, Nipam H. Patel, Megan L. Hochstrasser · 2018 · Journal of Visualized Experiments
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A piggyBac-based toolkit for inducible genome editing in mammalian cells
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Targeted mutagenesis with sequence‐specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects
David May, Katalin Paldi, Fredy Altpeter · 2023 · The Plant Genome
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Narendranath Bhokisham, Ethan Laudermilch, Lindsay L. Traeger, Tonya D. Bonilla, Mercedes Ruíz-Estévez, Jordan R. Becker · 2023 · Cells
The Current State of Naïve Human Pluripotency
Benjamin T. Dodsworth, Rowan Flynn, Sally A. Cowley · 2015 · Stem Cells
CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement
Workie Anley Zegeye, Mesfin Tsegaw, Yingxin Zhang, Liyong Cao · 2022 · International Journal of Molecular Sciences
Personalised genome editing – The future for corneal dystrophies
Tara Moore, Kathleen A. Christie, John Marshall, M. Andrew Nesbit · 2018 · Progress in Retinal and Eye Research
Herpesviral lytic gene functions render the viral genome susceptible to novel editing by CRISPR/Cas9
Hyung Suk Oh, Werner Neuhausser, Pierce Eggan, Magdalena Angelova, Rory D. Kirchner, Kevin C. Eggan, David M. Knipe · 2019 · eLife
Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes
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Minimizing off-Target Mutagenesis Risks Caused by Programmable Nucleases
Kentaro Ishida, Peter Gee, Akitsu Hotta · 2015 · International Journal of Molecular Sciences
Keeping CRISPR/Cas on-Target
Muhammad Jamal, Faheem Ahmed Khan, Da Lin, Zeshan Habib, Jinxia Dai, Gang Cao · 2016 · Current Issues in Molecular Biology
GUIDEseq: a bioconductor package to analyze GUIDE-Seq datasets for CRISPR-Cas nucleases
Lihua Julie Zhu, Michael Lawrence, Ankit Gupta, Hervé Pagès, Alper Küçükural, Manuel G. Garber, Scot A. Wolfe · 2017 · BMC Genomics
Targeting DNA methyltransferases in non-small-cell lung cancer
Minnatallah Al-Yozbaki, Ibtissam Jabre, Naeem Hasan Syed, Cornelia M. Wilson · 2021 · Seminars in Cancer Biology
The AAV-mediated and RNA-guided CRISPR/Cas9 system for gene therapy of DMD and BMD
Wang, Jing-Zhang, Wu, Peng, Shi, Zhi-Min, Xu, Yan-Li, Liu, Zhi-Jun · 2017 · Brain and Development
Novel Genome-Editing Tools to Model and Correct Primary Immunodeficiencies
Lisa M. Ott de Bruin, Stefano Volpi, Kiran Musunuru · 2015 · Frontiers in Immunology
Optimization of Cas9 activity through the addition of cytosine extensions to single-guide RNAs
Masaki Kawamata, Hiroshi I. Suzuki, Ryota Kimura, Atsushi Suzuki · 2023 · Nature Biomedical Engineering
CRISPR‐Cas: From the Bacterial Adaptive Immune System to a Versatile Tool for Genome Engineering
Marion Kirchner, Sabine Schneider · 2015 · Angewandte Chemie International Edition
CRISPR‐Cas9 systems: versatile cancer modelling platforms and promising therapeutic strategies
Wanshun Wen, Zhi‐Min Yuan, Shijie Ma, Xu Jiang, Dongtang Yuan · 2015 · International Journal of Cancer
CRISPR-Cas9: from Genome Editing to Cancer Research
Si Chen, Heng Sun, Kai Miao, Chu‐Xia Deng · 2016 · International Journal of Biological Sciences
R-loop formation by dCas9 is mutagenic in Saccharomyces cerevisiae
Marian F. Laughery, Hannah C Mayes, Ivan K Pedroza, John J. Wyrick · 2018 · Nucleic Acids Research
A kinetic model predicts SpCas9 activity, improves off-target classification, and reveals the physical basis of targeting fidelity
Behrouz Eslami-Mossallam, Misha Klein, Constantijn P. van der Smagt, Koen van der Sanden, Stephen K. Jones, John A. Hawkins, Ilya J. Finkelstein, Martin Depken · 2022 · Nature Communications
Paving the way towards precise and safe CRISPR genome editing
Paweł Śledziński, Magdalena Dabrowska, Mateusz Nowaczyk, Marta Olejniczak · 2021 · Biotechnology Advances
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A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila
Xu Jiang, Xingjie Ren, Jin Sun, Xia Wang, Huanhuan Qiao, Bowen Xu, Luping Liu, Jian‐Quan Ni · 2015 · Journal of genetics and genomics/Journal of Genetics and Genomics
Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress
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