Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors

Multiplex Genome Engineering Using CRISPR/Cas Systems

Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339

RNA-Guided Human Genome Engineering via Cas9

Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210

Genome-scale CRISPR-Cas9 knockout screening in human cells

Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench, J. G., et al. (2014) · Science · doi:10.1126/science.1247005 · Cited by 5,234

DNA targeting specificity of RNA-guided Cas9 nucleases

Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741

PEAR: a fast and accurate Illumina Paired-End reAd mergeR

Zhang, J., Kobert, K., Flouri, T., & Stamatakis, A. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btt593 · Cited by 4,497

Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

Ran, F. A., Hsu, P. D., Lin, C.-Y., Gootenberg, J. S., Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., et al. (2013) · Cell · doi:10.1016/j.cell.2013.08.021 · Cited by 3,299

High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells

Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon, D., Joung, J. K., & Sander, J. D. (2013) · Nature Biotechnology · doi:10.1038/nbt.2623 · Cited by 3,292

Genetic screens in human cells using the CRISPR-Cas9 system

Wang, T., Wei, J. J., Sabatini, D. M., & Lander, E. S. (2014) · Science · doi:10.1126/science.1246981 · Cited by 2,856

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

Mali, P., Aach, J., Stranges, P. B., Esvelt, K. M., Moosburner, M., Kosuri, S., Yang, L., & Church, G. M. (2013) · Nature Biotechnology · doi:10.1038/nbt.2675 · Cited by 1,752

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

Pattanayak, V., Lin, S., Guilinger, J. P., Ma, E., Doudna, J. A., & Liu, D. R. (2013) · Nature Biotechnology · doi:10.1038/nbt.2673 · Cited by 1,487

Nano-vectors for CRISPR/Cas9-mediated genome editing

Yang, P., Lee, A. Y.-T., Xue, J., Chou, S., Lee, C., Tseng, P., Zhang, T. X., Zhu, Y., Lee, J., Chiou, S., et al. (2022) · Nano Today · doi:10.1016/j.nantod.2022.101482 · Cited by 41

High-Throughput Silencing Using the CRISPR-Cas9 System: A Review of the Benefits and Challenges

Wade, M. R. (2015) · SLAS DISCOVERY · doi:10.1177/1087057115587916 · Cited by 39

Functional Genomic Strategies for Elucidating Human–Virus Interactions

Perreira, J. M., Meraner, P., & Brass, A. L. (2016) · Advances in virus research · doi:10.1016/bs.aivir.2015.11.001 · Cited by 39

CRISPR ‐Cas9 technology and its application in haematological disorders

Zhang, H., & McCarty, N. (2016) · British Journal of Haematology · doi:10.1111/bjh.14297 · Cited by 37

CRISPR–Cas9 Genetic Analysis of Virus–Host Interactions

Gebre, M. S., Nomburg, J., & Gewurz, B. E. (2018) · Viruses · doi:10.3390/v10020055 · Cited by 37

Impact of Genetic Variation on CRISPR-Cas Targeting

Canver, M. C., Joung, J. K., & Pinello, L. (2018) · The CRISPR Journal · doi:10.1089/crispr.2017.0016 · Cited by 36

Gene Editing Technologies to Target HBV cccDNA

Martínez, M. G., Smekalova, E. M., Combe, E., Gregoire, F. M., Zoulim, F., & Testoni, B. (2022) · Viruses · doi:10.3390/v14122654 · Cited by 37

Biomimetic Nanoparticle-Mediated Target Delivery of Hypoxia-Responsive Plasmid of Angiotensin-Converting Enzyme 2 to Reverse Hypoxic Pulmonary Hypertension

Yuan, R., Liu, M., Cheng, Y. H., Fang, Y., Zhu, X., Zhou, S., & Dong, M. (2023) · ACS Nano · doi:10.1021/acsnano.2c12190 · Cited by 35

Efficient generation of B2m-null pigs via injection of zygote with TALENs

Wang, Y., Du, Y., Zhou, X., Wang, L., Li, J., Wang, F., Huang, Z., Huang, X., & Hong Kui, W. E. I. (2016) · Scientific Reports · doi:10.1038/srep38854 · Cited by 35

Re-engineered RNA-Guided FokI-Nucleases for Improved Genome Editing in Human Cells

Havlicek, S., Shen, Y., Alpagu, Y., Bruntraeger, M., Zufir, N. B. M., Phuah, Z. Y., Fu, Z., Dunn, N. R., & Stanton, L. W. (2017) · Molecular Therapy · doi:10.1016/j.ymthe.2016.11.007 · Cited by 33

Use of genome-editing tools to treat sickle cell disease

Tasan, I., Jain, S., & Zhao, H. (2016) · Human Genetics · doi:10.1007/s00439-016-1688-0 · Cited by 36

Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus

Fujita, T., Yuno, M., & Fujii, H. (2016) · Scientific Reports · doi:10.1038/srep30485 · Cited by 34

CRISPR genetic screens to discover host–virus interactions

McDougall, W. M., Perreira, J. M., Reynolds, E. C., & Brass, A. L. (2018) · Current Opinion in Virology · doi:10.1016/j.coviro.2018.03.007 · Cited by 32

Precise Cas9 targeting enables genomic mutation prevention

Chavez, A., Pruitt, B. W., Tuttle, M. L., Shapiro, R. S., Cecchi, R. J., Winston, J., Turczyk, B. M., Tung, M., Collins, J. J., & Church, G. M. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1718148115 · Cited by 32

A Small Molecule-Controlled Cas9 Repressible System

Wu, Y., Yang, L., Chang, T., KANDEEL, F. R., & Yee, J. (2020) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2019.12.026 · Cited by 32

STING Gain-of-Function Disrupts Lymph Node Organogenesis and Innate Lymphoid Cell Development in Mice

Bennion, B. G., Croft, C. A., Ai, T. L., Qian, W. P., Menos, A. M., Miner, C. A., Frémond, M., Doisne, J., Andhey, P. S., Platt, D. J., et al. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107771 · Cited by 35

Benchmarking and integrating genome-wide CRISPR off-target detection and prediction

Yan, J., Xue, D., Chuai, G., Gao, Y., Zhang, G., & Liu, Q. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa930 · Cited by 35

Applications of Alternative Nucleases in the Age of CRISPR/Cas9

Guha, T. K., & Edgell, D. R. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18122565 · Cited by 37

Deep sampling of gRNA in the human genome and deep-learning-informed prediction of gRNA activities

Zhang, H., Yan, J., Lu, Z., Zhou, Y., Zhang, Q., Cui, T., Li, Y., Chen, H., & Ma, L. (2023) · Cell Discovery · doi:10.1038/s41421-023-00549-9 · Cited by 35

Data imbalance in CRISPR off-target prediction

Gao, Y., Chuai, G., Yu, W., Qu, S., & Liu, Q. (2019) · Briefings in Bioinformatics · doi:10.1093/bib/bbz069 · Cited by 31

The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing

Vicente, M. M., Chaves‐Ferreira, M., Jorge, J. M. P., Proença, J. T., & Barreto, V. M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.718466 · Cited by 31

Illuminating the genome-wide activity of genome editors for safe and effective therapeutics

Cheng, Y., & Tsai, S. Q. (2018) · Genome biology · doi:10.1186/s13059-018-1610-2 · Cited by 32

DL-CRISPR: A Deep Learning Method for Off-Target Activity Prediction in CRISPR/Cas9 With Data Augmentation

Zhang, Y., Long, Y., Yin, R., & Kwoh, C. K. (2020) · IEEE Access · doi:10.1109/access.2020.2989454 · Cited by 31

Genome Engineering with TALE and CRISPR Systems in Neuroscience

Lee, H. B., Sundberg, B. N., Sigafoos, A. N., & Clark, K. J. (2016) · Frontiers in Genetics · doi:10.3389/fgene.2016.00047 · Cited by 33

Recent challenges and advances in genetically-engineered cell therapy

Yong, S., Chung, J. Y., Song, Y., & Kim, Y. (2017) · Journal of Pharmaceutical Investigation · doi:10.1007/s40005-017-0381-1 · Cited by 30

A versatile CRISPR/Cas9 system off-target prediction tool using language model

Du, W., Zhao, L., Diao, K., Zheng, Y., Yang, Q., Zhu, Z., Zhu, X., & Tang, D. (2025) · Communications Biology · doi:10.1038/s42003-025-08275-6 · Cited by 38

Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases

Huang, H., Hu, Y., Huang, G., Ma, S., Feng, J., Wang, D., Lin, Y., Zhou, J., & Rong, Z. (2021) · Communications Biology · doi:10.1038/s42003-021-02351-3 · Cited by 28

A risk-based approach for cell line development, manufacturing and characterization of genetically engineered, induced pluripotent stem cell–derived allogeneic cell therapies

Dashnau, J. L., Xue, Q., Nelson, M., Law, E., Cao, L., & Hei, D. J. (2022) · Cytotherapy · doi:10.1016/j.jcyt.2022.08.001 · Cited by 30

Progress and Challenges in HIV-1 Vaccine Research: A Comprehensive Overview

Boomgarden, A. C., & Upadhyay, C. (2025) · Vaccines · doi:10.3390/vaccines13020148 · Cited by 28

Advances and applications of environmental stress adaptation research

Hawkins, L. J., & Storey, K. B. (2019) · Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology · doi:10.1016/j.cbpa.2019.110623 · Cited by 35

Genome-Wide Analysis of Off-Target CRISPR/Cas9 Activity in Single-Cell-Derived Human Hematopoietic Stem and Progenitor Cell Clones

Smith, R. H., Chen, Y.-C., Seifuddin, F., Hupalo, D. N., Alba, C., Reger, R. N., Tian, X., Araki, D., Dalgard, C. L., Childs, R. W., et al. (2020) · Genes · doi:10.3390/genes11121501 · Cited by 26

Tissue-specific gene targeting using CRISPR/Cas9

Ablain, J., & Zon, L. I. (2016) · Methods in cell biology · doi:10.1016/bs.mcb.2016.03.004 · Cited by 30

Progress in the application of CRISPR: From gene to base editing

Wu, W., Yang, Y., & Lei, H. (2018) · Medicinal Research Reviews · doi:10.1002/med.21537 · Cited by 28

When genome editing goes off-target

Kempton, H. R., & Qi, L. S. (2019) · Science · doi:10.1126/science.aax1827 · Cited by 30

Integration-defective lentiviral vector mediates efficient gene editing through homology-directed repair in human embryonic stem cells

Wang, Y., Wang, Y., Chang, T., Huang, H., & Yee, J.-K. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkw1057 · Cited by 26

Innovative Precision Gene‐Editing Tools in Personalized Cancer Medicine

Dai, X., Blancafort, P., Wang, P., Sgro, A., Thompson, E. W., & Ostrikov, K. (2020) · Advanced Science · doi:10.1002/advs.201902552 · Cited by 28

A Transgenic Core Facility’s Experience in Genome Editing Revolution

Yuan, C. L., & Hu, Y. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-63904-8_4 · Cited by 26

Targeting Specificity of the CRISPR/Cas9 System

Tasan, I., & Zhao, H. (2017) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00270 · Cited by 26

Transformer-based anti-noise models for CRISPR-Cas9 off-target activities prediction

Guan, Z., & Jiang, Z. (2023) · Briefings in Bioinformatics · doi:10.1093/bib/bbad127 · Cited by 27

Extru-seq: A method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches

Kwon, J., Kim, M., Hwang, W., Jo, A., Hwang, G.-H., Jung, M., Kim, U. G., Cui, G., Kim, H., Eom, J.-H., et al. (2023) · Genome biology · doi:10.1186/s13059-022-02842-4 · Cited by 24

Human Induced Pluripotent Stem Cell NEUROG2 Dual Knockin Reporter Lines Generated by the CRISPR/Cas9 System

Li, S., Xue, H., Wu, J., Rao, M. S., Kim, D., Deng, W., & Liu, Y. (2015) · Stem Cells and Development · doi:10.1089/scd.2015.0131 · Cited by 24

A pre-screening FISH-based method to detect CRISPR/Cas9 off-targets in mouse embryonic stem cells

Paulis, M., Castelli, A., Lizier, M., Susani, L., Lucchini, F., VILLA, A. M., & Vezzoni, P. (2015) · Scientific Reports · doi:10.1038/srep12327 · Cited by 24

Comprehensive off-target analysis of dCas9-SAM-mediated HIV reactivation via long noncoding RNA and mRNA profiling

Zhang, Y., Arango, G., Li, F., Xiao, X., Putatunda, R., Yu, J., Yang, X. F., Wang, H., Watson, L. T., Zhang, L., et al. (2018) · BMC Medical Genomics · doi:10.1186/s12920-018-0394-2 · Cited by 25

CRISPR-Cas9: A multifaceted therapeutic strategy for cancer treatment

Kaushik, I., Ramachandran, S., & Srivastava, S. (2019) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2019.04.018 · Cited by 25

THE JOURNEY OF CRISPR-CAS9 FROM BACTERIAL DEFENSE MECHANISM TO A GENE EDITING TOOL IN BOTH ANIMALS AND PLANTS

Tahir, T., Ali, Q., Rashid, M., & Malik, A. (2020) · Biological and Clinical Sciences Research Journal · doi:10.54112/bcsrj.v2020i1.17 · Cited by 23

Genome Editing in Neurosciences

Jaenisch, R., Gage, F. H., & Zhang, F. (2017) · Research and perspectives in neurosciences · doi:10.1007/978-3-319-60192-2 · Cited by 23

Efficient generation of GGTA1-null Diannan miniature pigs using TALENs combined with somatic cell nuclear transfer

Cheng, W., Zhao, H., Yu, H., Xin, J., Wang, J., Zeng, L., Yuan, Z., Qing, Y., Li, H., Jia, B., et al. (2016) · Reproductive Biology and Endocrinology · doi:10.1186/s12958-016-0212-7 · Cited by 23

An unbiased method for evaluating the genome-wide specificity of base editors in rice

Jin, S., Gao, Q., & Gao, C. (2020) · Nature Protocols · doi:10.1038/s41596-020-00423-y · Cited by 24

Prediction of sgRNA Off-Target Activity in CRISPR/Cas9 Gene Editing Using Graph Convolution Network

Vinodkumar, P. K., Ozcinar, C., & Anbarjafari, G. (2021) · Entropy · doi:10.3390/e23050608 · Cited by 22

Current landscape of gene‐editing technology in biomedicine: Applications, advantages, challenges, and perspectives

Zhou, W., Yang, J., Zhang, Y., Hu, X., & Wang, W. (2022) · MedComm · doi:10.1002/mco2.155 · Cited by 23

Single-Molecule Insight Into Target Recognition by CRISPR–Cas Complexes

Rutkauskas, M., Krivoy, A., Szczelkun, M. D., Rouillon, C., & Seidel, R. (2016) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2016.10.001 · Cited by 20

INSERT-seq enables high-resolution mapping of genomically integrated DNA using Nanopore sequencing

Ivančić, D., Mir-Pedrol, J., Jaraba-Wallace, J., Rafel, N., Sánchez-Mejías, A., & Güell, M. (2022) · Genome biology · doi:10.1186/s13059-022-02778-9 · Cited by 22

CRISPR-Cas and CRISPR-based screening system for precise gene editing and targeted cancer therapy

Qin, M., Deng, C., Wen, L., Luo, G., & Meng, Y. (2024) · Journal of Translational Medicine · doi:10.1186/s12967-024-05235-2 · Cited by 20

Gene editing toward the use of autologous therapies in recessive dystrophic epidermolysis bullosa

Perdoni, C., Osborn, M. J., & Tolar, J. (2015) · Translational research · doi:10.1016/j.trsl.2015.05.008 · Cited by 20

Quantifying CRISPR off-target effects

Gkazi, S. A. (2019) · Emerging Topics in Life Sciences · doi:10.1042/etls20180146 · Cited by 20

TraFo-CRISPR: Enhanced Genome Engineering by Transient Foamy Virus Vector-Mediated Delivery of CRISPR/Cas9 Components

Lindel, F., Dodt, C., Weidner, N., Noll, M., Bergemann, F., Behrendt, R., Fischer, S., Dietrich, J., Cartellieri, M., Hamann, M. V., et al. (2019) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2019.10.006 · Cited by 22

A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a ‘self-cleaving’ GFP-expression plasmid

Tálas, A., Kulcsár, P. I., Weinhardt, N., Borsy, A., Tóth, E., Szebényi, K., Krausz, S. L., Huszár, K., Vida, I., Sturm, Á., et al. (2017) · DNA Research · doi:10.1093/dnares/dsx029 · Cited by 19

CRISPR/Cas9‐based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells

Gupta, N. R., Susa, K., Yoda, Y., Bonventre, J. V., Valerius, M. T., & Morizane, R. (2018) · Current Protocols in Stem Cell Biology · doi:10.1002/cpsc.50 · Cited by 18

Genome-Wide Profiling of DNA Double-Strand Breaks by the BLESS and BLISS Methods

Mirzazadeh, R., Kallas, T., Bienko, M., & Crosetto, N. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7306-4_14 · Cited by 18

CRISPR to the Rescue: Advances in Gene Editing for the FMR1 Gene

Yrigollen, C. M., & Davidson, B. L. (2019) · Brain Sciences · doi:10.3390/brainsci9010017 · Cited by 19

Dissecting Tissue-Specific Super-Enhancers by Integrating Genome-Wide Analyses and CRISPR/Cas9 Genome Editing

Yoo, K. H., Hennighausen, L. G., & Shin, H. Y. (2018) · Journal of Mammary Gland Biology and Neoplasia · doi:10.1007/s10911-018-9417-z · Cited by 18

CRISPR/Cas: An intriguing genomic editing tool with prospects in treating neurodegenerative diseases

Rahman, S., Datta, M., Kim, J., & Jan, A. T. (2019) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2019.05.014 · Cited by 19

Comprehensive analysis of off-target and on-target effects resulting from liver-directed CRISPR-Cas9-mediated gene targeting with AAV vectors

Singh, K., Fronza, R., Evens, H., Chuah, M., & VandenDriessche, T. (2024) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2024.101365 · Cited by 19

Integrase deficient lentiviral vector: prospects for safe clinical applications

Yew, C.-H. T., Gurumoorthy, N., Nordin, F., Tye, G. J., Zaman, W. S. W. K., Tan, J. J., & Ng, M. H. (2022) · PeerJ · doi:10.7717/peerj.13704 · Cited by 20

DNA as the main target in radiotherapy—a historical overview from first isolation to anti-tumour immune response

Frey, B., Borgmann, K., Jost, T., Greve, B., Oertel, M., Micke, O., & Eckert, F. (2023) · Strahlentherapie und Onkologie · doi:10.1007/s00066-023-02122-5 · Cited by 18

CRISPR, animals, and FDA oversight: Building a path to success

Epstein, L. R., Lee, S. S., Miller, M. F., & Lombardi, H. A. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2004831117 · Cited by 17

Multi-reporter selection for the design of active and more specific zinc-finger nucleases for genome editing

Oakes, B. L., Xia, D. F., Rowland, E. F., Xu, D. J., Ankoudinova, I., Borchardt, J. S., Zhang, L., Li, P. W. F., Miller, J. C., Rebar, E. J., et al. (2016) · Nature Communications · doi:10.1038/ncomms10194 · Cited by 16

Precision in Action: The Role of Clustered Regularly Interspaced Short Palindromic Repeats/Cas in Gene Therapies

Banda, A., Impomeni, O., Singh, A., Baloch, A. R., Hu, W., & Jaijyan, D. K. (2024) · Vaccines · doi:10.3390/vaccines12060636 · Cited by 16

Current and future prospects for hemophilia gene therapy

Ward, P., & Walsh, C. (2016) · Expert Review of Hematology · doi:10.1080/17474086.2016.1182859 · Cited by 16

Gene editing in T cell therapy

Zhang, Y., Mu, W., & Wang, H. (2017) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2017.09.002 · Cited by 18

A Prospective Treatment Option for Lysosomal Storage Diseases: CRISPR/Cas9 Gene Editing Technology for Mutation Correction in Induced Pluripotent Stem Cells

Christensen, C. L., & Choy, F. Y. M. (2017) · Diseases · doi:10.3390/diseases5010006 · Cited by 16

Permutation-Based Causal Structure Learning with Unknown Intervention TargetsPreprint

Squires, C., Wang, Y., & Uhler, C. (2019) · arXiv (Cornell University) · doi:10.48550/arxiv.1910.09007 · Cited by 16

Applications of Innovation Technologies for Personalized Cancer Medicine: Stem Cells and Gene-Editing Tools

Hasanzadeh, A., Ebadati, A., Dastanpour, L., Aref, A. R., Zangabad, P. S., Kalbasi, A., Dai, X., Mehta, G., Ghasemi, A., Fatahi, Y., et al. (2023) · ACS Pharmacology & Translational Science · doi:10.1021/acsptsci.3c00102 · Cited by 16

Applications of the CRISPR/Cas9 system in murine cancer modeling

Zuckermann, M., Kawauchi, D., & Gronych, J. (2016) · Briefings in Functional Genomics · doi:10.1093/bfgp/elw021 · Cited by 15

Designing Safer CRISPR/Cas9 Therapeutics for HIV: Defining Factors That Regulate and Technologies Used to Detect Off-Target Editing

Sullivan, N. T., Allen, A. G., Atkins, A., Chung, C.-H., Dampier, W., Nonnemacher, M. R., & Wigdahl, B. L. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01872 · Cited by 16

CRISPR/Cas9; A robust technology for producing genetically engineered plants

Farooq, R., Hussain, K., Nazir, S., Javed, M. R., & Masood, N. (2018) · Cellular and Molecular Biology · doi:10.14715/cmb/2018.64.14.6 · Cited by 15

The first genetically gene‐edited babies: It's “irresponsible and too early”

Ma, Y., Zhang, L., & Qin, C. (2019) · Animal Models and Experimental Medicine · doi:10.1002/ame2.12052 · Cited by 15

Cell-Penetrating Peptides and CRISPR-Cas9: A Combined Strategy for Human Genetic Disease Therapy

de Morais, C. C. P. D. L., Correia, E. M., Bonamino, M., & de Vasconcelos, Z. F. M. (2024) · Human Gene Therapy · doi:10.1089/hum.2024.020 · Cited by 15

TINC— A Method to Dissect Regulatory Complexes at Single-Locus Resolution— Reveals an Extensive Protein Complex at the Nanog Promoter

Knaupp, A. S., Mohenska, M., Larcombe, M. R., Ford, E., Lim, S. M., Wong, K. C. Y., Chen, J., Firas, J., Huang, C., Liu, X., et al. (2020) · Stem Cell Reports · doi:10.1016/j.stemcr.2020.11.005 · Cited by 14

Emerging Technologies for Genome-Wide Profiling of DNA Breakage

Rybin, M. J., Ramic, M., Ricciardi, N. R., Kapranov, P., Wahlestedt, C., & Zeier, Z. R. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2020.610386 · Cited by 15

New hope and promise with CRISPR-Cas9 technology for the treatment of HIV

Hassan, H. M., Zubair, A., Helal, M. H., Almagharbeh, W. T., & Elmagzoub, R. M. (2025) · Functional & Integrative Genomics · doi:10.1007/s10142-025-01613-1 · Cited by 14

Non-clinical safety assessment of novel drug modalities: Genome safety perspectives on viral-, nuclease- and nucleotide-based gene therapies

Lemmens, M., Dorsheimer, L., Zeller, A., & Dietz, Y. (2024) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2024.503767 · Cited by 15

Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells

Zhang, C., He, X., Kwok, Y. K. Y., Wang, F., Xue, J., Zhao, H., Suen, K. W., Wang, C. C., Ren, J., Chen, G. G., et al. (2018) · BMC Biology · doi:10.1186/s12915-018-0616-2 · Cited by 13

Divalent cations promote TALE DNA-binding specificity

Cuculis, L., Zhao, C., Abil, Z., Zhao, H., Shukla, D., & Schroeder, C. M. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz1174 · Cited by 13

Low dose ionizing radiation strongly stimulates insertional mutagenesis in a γH2AX dependent manner

Zelensky, A. N., Schoonakker, M., Brandsma, I., Tijsterman, M., van Gent, D. C., Essers, J., & Kanaar, R. (2020) · PLoS Genetics · doi:10.1371/journal.pgen.1008550 · Cited by 14

A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets

Kulcsár, P. I., Tálas, A., Ligeti, Z., Tóth, E., Rakvács, Z., Bartos, Z., Krausz, S. L., Welker, Á., Végi, V. L., Huszár, K., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-41393-5 · Cited by 14

A novel luciferase knock-in reporter system for studying transcriptional regulation of the human Sox2 gene

Xiao, D., Zhang, W., Li, Y., Liu, K., Zhao, J., Sun, X., Shan, L., Mao, Q., & Xia, H. (2015) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2015.12.026 · Cited by 12

Synthetic biology approaches in cancer immunotherapy, genetic network engineering, and genome editing

Chakravarti, D., Cho, J. H., Weinberg, B. H., Wong, N. M. L., & Wong, W. W. (2016) · Integrative Biology · doi:10.1039/c5ib00325c · Cited by 13

The CRISPR-Cas system – from bacterial immunity to genome engineering

Czarnek, M., & Bereta, J. (2016) · Postępy Higieny i Medycyny Doświadczalnej · doi:10.5604/17322693.1216379 · Cited by 13

A Practical Guide to Genome Editing Using Targeted Nuclease Technologies

Porter, S. N., Levine, R. M., & Pruett‐Miller, S. M. (2019) · Comprehensive physiology · doi:10.1002/cphy.c180022 · Cited by 18

The Evolution of Nucleic Acid–Based Diagnosis Methods from the (pre-)CRISPR to CRISPR era and the Associated Machine/Deep Learning Approaches in Relevant RNA Design

Chakraborty, S. S., Dutta, J. R., Ganesan, R., & Mináry, P. (2024) · Methods in molecular biology · doi:10.1007/978-1-0716-4079-1_17 · Cited by 11

Recent developments, challenges and opportunities in genome editing for crop science from a societal perspective

Venkataraman, S., Zaruk, D., & Hefferon, K. L. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1568072 · Cited by 13

Applications of Genome Editing Tools in Stem Cells Towards Regenerative Medicine: An Update

Kues, W. A., Kumar, D., Selokar, N. L., & Talluri, T. R. (2021) · Current Stem Cell Research & Therapy · doi:10.2174/1574888x16666211124095527 · Cited by 11

TEG-Seq: An Ion Torrent-Adapted NGS Workflow for in Cellulo Mapping of CRISPR Specificity

Tang, P., Bo, D., Peng, L., Mozhayskiy, V., Potter, J., & Chesnut, J. D. (2018) · BioTechniques · doi:10.2144/btn-2018-0105 · Cited by 10

CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates

Yin, F., Liu, W., Chai, J., Lu, B., Murphy, R. W., & Luo, J. (2018) · Frontiers in Genetics · doi:10.3389/fgene.2018.00260 · Cited by 11

Development and challenges of using CRISPR-Cas9 system in mammalians

Khambhati, K., Gohil, N., Bhattacharjee, G., & Singh, V. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818140-9.00007-6 · Cited by 10

Dynamics of Target DNA Binding and Cleavage by Staphylococcus aureus Cas9 as Revealed by High-Speed Atomic Force Microscopy

Puppulin, L., Ishikawa, J., Sumino, A., Marchesi, A. S., Flechsig, H., Umeda, K., Kodera, N., Nishimasu, H., & Shibata, M. (2023) · ACS Nano · doi:10.1021/acsnano.2c10709 · Cited by 10

The Molecular Revolution in Cutaneous Biology: Emerging Landscape in Genomic Dermatology: New Mechanistic Ideas, Gene Editing, and Therapeutic Breakthroughs

Titeux, M., Izmiryan, A., & Hovnanian, A. (2017) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2016.08.038 · Cited by 9

Systems-level interference strategies to decipher host factors involved in bacterial pathogen interaction: from RNAi to CRISPRi

Québatte, M., & Dehio, C. (2017) · Current Opinion in Microbiology · doi:10.1016/j.mib.2017.08.002 · Cited by 11

SeqCor: correct the effect of guide RNA sequences in clustered regularly interspaced short palindromic repeats/Cas9 screening by machine learning algorithm

Liu, X., Yang, Y., Qiu, Y., Reyad‐ul‐Ferdous, M., Ding, Q., & Wang, Y. (2020) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2020.10.007 · Cited by 10

Detection of CRISPR/Cas9-Generated off-target effect by integration-defective lentiviral vector

Wang, X., Wu, Y., & Yee, J.-K. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-0687-2_14 · Cited by 5

CRISPR off-target detection

(2015) · Nature Methods · doi:10.1038/nmeth.3301

Unbiased Detection of Off-target Cleavage by CRISPR/Cas9 and TALENs Using Integration-defective Lentiviral Vectors

Wu, Y., & Yee, J. (2018) · Cambridge University Press eBooks · doi:10.1017/9781316756300.004

Off-Target Analysis in Gene Editing and Applications for Clinical Translation of CRISPR/Cas9 in HIV-1 Therapy

Atkins, A., Chung, C.-H., Allen, A. G., Dampier, W., Gurrola, T. E., Sariyer, I. K., Nonnemacher, M. R., & Wigdahl, B. L. (2021) · Frontiers in Genome Editing · doi:10.3389/fgeed.2021.673022 · Cited by 78

SpacerScope: Binary-vectorized, genome-wide off-target profiling for RNA-guided nucleases without prior candidate-site biasPreprint

Qu, Y., Wang, Y., Wang, Y., Tang, H., & Chen, Q. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.03.28.715005