Cited by 22Neurogenesis and neuroplasticity mechanismsMicroRNA in disease regulationDevelopmental Biology and Gene Regulation

MicroRNA profiling of mouse cortical progenitors and neurons reveals miR-486-5p as a regulator of neurogenesis

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Dori, Martina, Cavalli, Daniel, Lesche, Mathias, Massalini, Simone, Alieh, Leila Haj Abdullah, de Toledo, Beatriz Cardoso, Khudayberdiev, Sharof, Schratt, Gerhard +2 more·2020·Development

Published version of a preprint

Abstract

MicroRNAs (miRNAs) are short (∼22 nt) single-stranded non-coding RNAs that regulate gene expression at the post-transcriptional level. Over recent years, many studies have extensively characterized the involvement of miRNA-mediated regulation in neurogenesis and brain development. However, a comprehensive catalog of cortical miRNAs expressed in a cell-specific manner in progenitor types of the developing mammalian cortex is still missing. Overcoming this limitation, here we exploited a double reporter mouse line previously validated by our group to allow the identification of the transcriptional signature of neurogenic commitment and provide the field with the complete atlas of miRNA expression in proliferating neural stem cells, neurogenic progenitors and newborn neurons during corticogenesis. By extending the currently known list of miRNAs expressed in the mouse brain by over twofold, our study highlights the power of cell type-specific analyses for the detection of transcripts that would otherwise be diluted out when studying bulk tissues. We further exploited our data by predicting putative miRNAs and validated the power of our approach by providing evidence for the involvement of miR-486 in brain development.

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Authors
Dori, M., Cavalli, D., Lesche, M., Massalini, S., Alieh, L. H. A., de Toledo, B. C., Khudayberdiev, S., Schratt, G., Dahl, A., & Calegari, F.
Affiliations
  • Center for Regenerative Therapies Dresden (DE)
  • DRESDEN-concept Genome Center (DE)
  • CRTD – Center for Regenerative Therapies Dresden, School of Medicine, TU Dresden, Fetcherstrasse 105, 01307 Dresden, Germany
  • Philipps University of Marburg (DE)
Corresponding author
Calegari, Federico
Venue
Development
Published
2020-05-01
Volume
147
Issue
9
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
OpenAlex
W3016056682
Cited by
22
References
65
Citations by year
2026: 1 · 2025: 7 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 3
FWCI
0.98
Type
journal article
Language
en
Open access
Locations
  • Development (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Funders
  • Zentrum für Regenerative Therapien Dresden
  • Technische Universität Dresden
  • Deutsche Forschungsgemeinschaft (CA893/9-1)
  • Ministero dell'Istruzione, dell'Università e della Ricerca
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
miRNA expression profiling, miR-486-5p, neurogenesis, cortical progenitors, neural stem cells, newborn neurons, cortical development, cell type-specific gene expression, brain development
MeSH terms
Animals, Female, Immunohistochemistry, Mice, Inbred C57BL, Neurons, Blotting, Northern, In Situ Hybridization, Electroporation, Computational Biology, Gene Expression Profiling, MicroRNAs, Mice, Neurogenesis, Neural Stem Cells, High-Throughput Nucleotide Sequencing

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