Cited by 250Microbial Metabolic Engineering and BioproductionBacterial Genetics and BiotechnologyFungal and yeast genetics research

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Expression of Cloned Homologous Fermentative Genes in Clostridium Acetobutylicum ATCC 824

Mermelstein, Lee D., Welker, Neil E., Bennett, George N., Papoutsakis, Eleftherios T.·1992·Nature Biotechnology

DOI 10.1038/nbt0292-190

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Abstract

We have previously cloned the acetone-formation pathway gene, encoding acetoacetate decarboxylase (adc), and butyrate-formation pathway gene, encoding phosphotransbutyrylase (ptb), of Clostridium acetobutylicum ATCC 824 in Escherichia coli. Here we report their subcloning in Bacillus subtilis and transfer to strain ATCC 824 via electrotransformation, where the corresponding enzyme activities were expressed at elevated levels, using pFNK1, a new B. subtilis/C. acetobutylicum shuttle vector. Plasmid pFNK1 was used because shuttle vectors that function in E. coli were unable to electrotransform ATCC 824 unless they became deleted in the E. coli-plasmid regions. The difficulties with shuttle vectors that function in E. coli are probably due to the presence of a restriction endonuclease in ATCC 824. This endonuclease recognizes the sequence 5'-GCNGC-3', which is prevalent in E. coli plasmids but occurs infrequently in pFNK1 and C. acetobutylicum genes. Cloning of genes in C. acetobutylicum is critical for redirecting the cellular metabolism (metabolic engineering) as well as for genetic studies of this industrial organism.

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Detection of specific sequences among DNA fragments separated by gel electrophoresis
Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,245
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
Chang, A. C. Y., & Cohen, S. N. (1978) · Journal of Bacteriology · doi:10.1128/jb.134.3.1141-1156.1978 · Cited by 4,261
High efficiency transformation of E.coli by high voltage electroporation
Dower, W. J., Miller, J. F., & Ragsdale, C. W. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.13.6127 · Cited by 2,821
Acetone-butanol fermentation revisited
Jones, D. T., & Woods, D. (1986) · Microbiological Reviews · doi:10.1128/mr.50.4.484-524.1986 · Cited by 1,966
Simple and rapid method for isolating large plasmid DNA from lactic streptococci
Anderson, D. G., & McKay, L. L. (1983) · Applied and Environmental Microbiology · doi:10.1128/aem.46.3.549-552.1983 · Cited by 1,026
Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis
Simon, D. A., & Chopin, A. (1988) · Biochimie · doi:10.1016/0300-9084(88)90093-4 · Cited by 403
Restriction enzymes and their isoschizomers
Roberts, R. J. (1990) · Nucleic Acids Research · doi:10.1093/nar/18.suppl.2331 · Cited by 262
A cloning vector able to replicate in Escherichia coli and Streptococcus sanguis
Macrina, F. L., Tobian, J. A., Jones, K. R., Evans, R. P., & Clewell, D. B. (1982) · Gene · doi:10.1016/0378-1119(82)90025-7 · Cited by 248

Show all 31 references

Research works printed but not linked: 3

10.1016/0378-1097(88)90131-0 · DOI · Paper page
Petersen, D.J. 1991. Ph.D. Thesis. Rice University, HOuston.
Mahler I., 1980, J. Gen. Microbiol., 120, 259

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Cho, C., & Lee, S. Y. (2016) · Biotechnology and Bioengineering · doi:10.1002/bit.26077 · Cited by 58
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Authors: Mermelstein, L. D., Welker, N. E., Bennett, G. N., & Papoutsakis, E. T.
Affiliations: Northwestern University (US)
Rice University (US)
ORCID: Bennett, George N.
Papoutsakis, Eleftherios T.
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 1992-02
Volume: 10
Issue: 2
Pages: 190-195
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt0292-190
OpenAlex: W2009188183
Cited by: 249
References: 31
Citations by year: 2025: 4 · 2024: 5 · 2023: 4 · 2022: 6 · 2021: 7 · 2020: 6 · 2019: 12 · 2018: 10 · 2017: 4 · 2016: 17 · 2015: 8 · 2014: 18 · 2013: 14 · 2012: 12
FWCI: 2.91
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (unspecified)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords: Clostridium acetobutylicum, acetoacetate decarboxylase, acetone production, butyrate production, heterologous expression, Bacillus subtilis, shuttle vector, electrotransformation, restriction enzymes, metabolic engineering
MeSH terms: Bacillus subtilis, Carboxy-Lyases, Cloning, Molecular, Clostridium, DNA, Bacterial, Escherichia coli, Fermentation, Genes, Bacterial, Genetic Vectors, Phosphate Acetyltransferase, Plasmids, Restriction Mapping

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Expression of Cloned Homologous Fermentative Genes in Clostridium Acetobutylicum ATCC 824

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