Engineering Clostridium strain to accept unmethylated DNA
Open accessAbstract
It is difficult to genetically manipulate the medically and biotechnologically important genus Clostridium due to the existence of the restriction and modification (RM) systems. We identified and engineered the RM system of a model clostridial species, C. acetobutylicum, with the aim to allow the host to accept the unmethylated DNA efficiently. A gene CAC1502 putatively encoding the type II restriction endonuclease Cac824I was identified from the genome of C. acetobutylicum DSM1731, and disrupted using the ClosTron system based on group II intron insertion. The resulting strain SMB009 lost the type II restriction endonuclease activity, and can be transformed with unmethylated DNA as efficiently as with methylated DNA. The strategy reported here makes it easy to genetically modify the clostridial species using unmethylated DNA, which will help to advance the understanding of the clostridial physiology from the molecular level.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Genetic disruption of restriction endonuclease in non-acetogen C. acetobutylicum and C. cellulolyticum resulted in significantly improved transformation efficiency using unmethylated plasmids.
“For instance, genetic disruption of restriction endonuclease in non-acetogen C. acetobutylicum (Dong et al., 2010) and C. cellulolyticum (Cui et al., 2012) resulted in significantly improved transformation efficiency using unmethylated plasmids.”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Dong et al., 2010
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589 · see citing works
Bergey's Manual of Systematic Bacteriology
(1990) · Annals of Internal Medicine · doi:10.7326/0003-4819-112-5-391_3 · Cited by 15,633 · see citing works
Bergey's Manual of Systematic Bacteriology
Kersters, K., & Vancanneyt, M. (2005) · Ghent University Academic Bibliography (Ghent University) · Cited by 2,257 · see citing works
Mechanisms of, and Barriers to, Horizontal Gene Transfer between Bacteria
Thomas, C. M., & Nielsen, K. M. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1234 · Cited by 2,194 · see citing works
Acetone-butanol fermentation revisited
Jones, D. T., & Woods, D. (1986) · Microbiological Reviews · doi:10.1128/mr.50.4.484-524.1986 · Cited by 1,966 · see citing works
Bacterial gene transfer by natural genetic transformation in the environment
Lorenz, M. G., & Wackernagel, W. (1994) · Microbiological Reviews · doi:10.1128/mr.58.3.563-602.1994 · Cited by 1,413 · see citing works
Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences
Rehner, S. A., & Samuels, G. J. (1994) · Mycological Research · doi:10.1016/s0953-7562(09)80409-7 · Cited by 1,139 · see citing works
Nölling, J., Breton, G., Omelchenko, M. V., Makarova, K. S., Zeng, Q., Gibson, R., Lee, H. M., Dubois, J., Qiu, D., Hitti, J., et al. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.16.4823-4838.2001 · Cited by 767 · see citing works
A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
Roberts, R. J. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg274 · Cited by 755 · see citing works
The ClosTron: a universal gene knock-out system for the genus Clostridium
Heap, J. T., Pennington, O. J., Cartman, S. T., Carter, G. P., & Minton, N. P. (2007) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2007.05.021 · Cited by 663 · see citing works
Narrative Review: The New Epidemic of Clostridium difficile–Associated Enteric Disease
Bartlett, J. G. (2006) · Annals of Internal Medicine · doi:10.7326/0003-4819-145-10-200611210-00008 · Cited by 592 · see citing works
van der Rest, M. E., Lange, C. M., & Douwe Molenaar, B. (1999) · Applied Microbiology and Biotechnology · doi:10.1007/s002530051557 · Cited by 467 · see citing works
Sakaguchi, G. (1982) · Pharmacology & Therapeutics · doi:10.1016/0163-7258(82)90061-4 · Cited by 369 · see citing works
Elhai, J., Vepritskiy, A., Muro-Pastor, A. M., Flores, E., & Wolk, C. P. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.6.1998-2005.1997 · Cited by 334 · see citing works
Mermelstein, L. D., & Papoutsakis, E. T. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.4.1077-1081.1993 · Cited by 291 · see citing works
REBASE—enzymes and genes for DNA restriction and modification
Roberts, R., Vincze, T., Pósfai, J., & Macelis, D. (2007) · Nucleic Acids Res. · doi:10.1093/nar/gkl891 · Cited by 269 · see citing works
Purdy, D., O'Keeffe, T. A. T., Elmore, M., Herbert, M., McLeod, A., Bokori‐Brown, M., Ostrowski, A., & Minton, N. P. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.03134.x · Cited by 255 · see citing works
Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824
Mermelstein, L. D., Welker, N. E., Bennett, G. N., & Papoutsakis, E. T. (1992) · Nature Biotechnology · doi:10.1038/nbt0292-190 · Cited by 249 · see citing works
Harris, L. M., Welker, N. E., & Papoutsakis, E. T. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.13.3586-3597.2002 · Cited by 230 · see citing works
Hirsch, A. K. H., & Grinsted, E. (1954) · Journal of Dairy Research · doi:10.1017/s0022029900007196 · Cited by 222 · see citing works
Green, E. M., Boynton, Z. L., Harris, L. M., Rudolph, F. B., Papoutsakis, E. T., & Bennett, G. N. (1996) · Microbiology · doi:10.1099/13500872-142-8-2079 · Cited by 216 · see citing works
Perůtka, J., Wang, W., Goerlitz, D. S., & Lambowitz, A. M. (2003) · Journal of Molecular Biology · doi:10.1016/j.jmb.2003.12.009 · Cited by 196 · see citing works
Dürre, P. (2005) · CRC Press eBooks · doi:10.1201/9780203489819 · Cited by 178 · see citing works
Antisense RNA Strategies for Metabolic Engineering of Clostridium acetobutylicum
Desai, R. P., & Papoutsakis, E. T. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.3.936-945.1999 · Cited by 172 · see citing works
Shao, L., Hu, S., Yang, Y., Gu, Y., Chen, J., Yang, Y., Jiang, W., & Yang, S. (2007) · Cell Research · doi:10.1038/cr.2007.91 · Cited by 165 · see citing works
Immigration control of DNA in bacteria: self versus non-self
Murray, N. E. (2002) · Microbiology · doi:10.1099/00221287-148-1-3 · Cited by 162 · see citing works
Kawabata, H., Norris, S. J., & Watanabe, H. (2004) · Infection and Immunity · doi:10.1128/iai.72.12.7147-7154.2004 · Cited by 160 · see citing works
Zhong, J. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg248 · Cited by 147 · see citing works
Tummala, S. B., Junne, S. G., & Papoutsakis, E. T. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.12.3644-3653.2003 · Cited by 145 · see citing works
The α‐toxin of Clostridium septicum is essential for virulence
Kennedy, C. L., Krejany, E. O., Young, L. F., O’Connor, J. R., Awad, M. M., Boyd, R. L., Emmins, J. J., Lyras, D., & Rood, J. I. (2005) · Molecular Microbiology · doi:10.1111/j.1365-2958.2005.04774.x · Cited by 136 · see citing works
Improvement of bacterial transformation efficiency using plasmid artificial modification
Yasui, K., Kano, Y., Tanaka, K. Y., Watanabe, K., Shimizu-Kadota, M., Yoshikawa, H., & Suzuki, T. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn884 · Cited by 129 · see citing works
Electroporation-mediated transformation of lysostaphin-treated Clostridium perfringens
Scott, P. T., & Rood, J. I. (1989) · Gene · doi:10.1016/0378-1119(89)90059-0 · Cited by 119 · see citing works
Introduction to the Physiology and Biochemistry of the Genus Clostridium
Andreesen, J. R., Bahl, H., & Gottschalk, G. (1989) · Springer eBooks · doi:10.1007/978-1-4757-9718-3_2 · Cited by 118 · see citing works
Multiple mechanisms controlling carbon metabolism in bacteria
Saier, M. H. (1998) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19980420)58:2/3 · Cited by 97 · see citing works
Transferable tetracycline resistance in Clostridium perfringens strains of porcine origin
Rood, J. I. (1983) · Canadian Journal of Microbiology · doi:10.1139/m83-193 · Cited by 80 · see citing works
Iwai, M., Katoh, H., Katayama, M., & Ikeuchi, M. (2004) · Plant and Cell Physiology · doi:10.1093/pcp/pch015 · Cited by 80 · see citing works
Products of Anaerobic 2,4,6-Trinitrotoluene (TNT) Transformation by Clostridium bifermentans
Lewis, T. A., Goszczyński, S., Crawford, R. L., Korus, R. A., & Admassu, W. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.12.4669-4674.1996 · Cited by 74 · see citing works
DNA methyltransferase genes of Bacillus subtilis phages: comparison of their nucleotide sequences
Tran-Betcke, A., Behrens, B., Noyer-Weidner, M., & Trautner, T. A. (1986) · Gene · doi:10.1016/0378-1119(86)90153-8 · Cited by 66 · see citing works
The Role of PerR in O2-Affected Gene Expression ofClostridium acetobutylicum
Hillmann, F., Döring, C., Riebe, O., Ehrenreich, A., Fischer, R., & Bahl, H. (2009) · Journal of Bacteriology · doi:10.1128/jb.00351-09 · Cited by 65 · see citing works
DNA restriction is a barrier to natural transformation in Pseudomonas stutzeri JM300
Berndt, C., Meier, P., & Wackernagel, W. (2003) · Microbiology · doi:10.1099/mic.0.26033-0 · Cited by 63 · see citing works
Inactivation of an Aldehyde/Alcohol Dehydrogenase Gene from Clostridium acetobutylicum ATCC 824
Green, E. M., & Bennett, G. N. (1996) · Humana Press eBooks · doi:10.1007/978-1-4612-0223-3_19 · Cited by 59 · see citing works
Yao, J., & Lambowitz, A. M. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.02829-06 · Cited by 56 · see citing works
Increasing DNA Transfer Efficiency by Temporary Inactivation of Host Restriction
Edwards, R. A., Helm, R. A., & Maloy, S. (1999) · BioTechniques · doi:10.2144/99265st02 · Cited by 49 · see citing works
Bayliss, C. D., Callaghan, M. J., & Moxon, E. R. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl568 · Cited by 43 · see citing works
Ohshima, H., Matsuoka, S., Asai, K., & Sadaie, Y. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.2.381-389.2002 · Cited by 40 · see citing works
Vector Construction, Transformation, and Gene Amplification in Clostridium acetobutylicum ATCC 824a
Lee, S. Y., Mermelstein, L. D., Bennett, G. N., & Papoutsakis, E. T. (1992) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1992.tb42572.x · Cited by 35 · see citing works
Moser, D. P., Zarka, D. G., & Kallas, T. (1993) · Archives of Microbiology · doi:10.1007/bf00249129 · Cited by 29 · see citing works
Common patterns in type II restriction enzyme binding sites
Nikolajewa, S. (2005) · Nucleic Acids Research · doi:10.1093/nar/gki575 · Cited by 12 · see citing works
Characterization of a methyl-specific restriction system inClostridium acetobutylicumstrain N1-4081
Azeddoug, H., Hubert, J., & Reysset, G. (1989) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1989.tb03682.x · Cited by 10 · see citing works
Azeddoug, H. (1989) · FEMS Microbiology Letters · doi:10.1016/0378-1097(89)90239-5 · Cited by 1 · see citing works
Flagged first, then by citations
1 of the 51 references couldn’t be matched to a known paper.
Research works printed but not linked: 1
- [30]Heap J Pennington O Cartman S Carter G Minton N 2007 The ClosTron: a universal gene knock-out system for the genus Clostridium. J Microbiol Meth 70 452 464 17658189
Cited by
Sorted by citations
Liew, F., Martin, M. E., Tappel, R. C., Heijstra, B. D., Mihalcea, C., & Köpke, M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00694 · Cited by 485 · see citing works
Metabolic engineering of Clostridium acetobutylicum: recent advances to improve butanol production
Lütke‐Eversloh, T., & Bahl, H. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.01.011 · Cited by 369 · see citing works
The methylomes of six bacteria
Murray, I. A., Clark, T. A., Morgan, R., Boitano, M., Anton, B. P., Luong, K., Fomenkov, A. I., Turner, S. W., Korlach, J., & Roberts, R. J. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks891 · Cited by 253 · see citing works
Li, Q., Chen, J., Minton, N. P., Zhang, Y., Wen, Z., Liu, J., Yang, H., Zeng, Z., Ren, X., Yang, J., et al. (2016) · Biotechnology Journal · doi:10.1002/biot.201600053 · Cited by 168 · see citing works
A Mimicking-of-DNA-Methylation-Patterns Pipeline for Overcoming the Restriction Barrier of Bacteria
Zhang, G., Wang, W., Deng, A., Sun, Z., Zhang, Y., Liang, Y., Che, Y., & Wen, T. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1002987 · Cited by 138 · see citing works
A roadmap for gene system development in Clostridium
Minton, N. P., Ehsaan, M., Humphreys, C. M., Little, G. T., Baker, J., Henstra, A. M., Liew, F. E., Kelly, M. L., Sheng, L., Schwarz, K. M., et al. (2016) · Anaerobe · doi:10.1016/j.anaerobe.2016.05.011 · Cited by 103 · see citing works
Pyne, M. E., Moo‐Young, M., Chung, D. A., & Chou, C.-H. P. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-50 · Cited by 89 · see citing works
Modifying the product pattern of Clostridium acetobutylicum
Lehmann, D., Hönicke, D., Ehrenreich, A., Schmidt, M., Weuster‐Botz, D., Bahl, H., & Lütke‐Eversloh, T. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3852-8 · Cited by 85 · see citing works
Lehmann, D., & Lütke‐Eversloh, T. (2011) · Metabolic Engineering · doi:10.1016/j.ymben.2011.04.006 · Cited by 84 · see citing works
Engineering Clostridium organisms as microbial cell-factories: challenges & opportunities
Charubin, K., Bennett, R. K., Fast, A. G., & Papoutsakis, E. T. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.07.012 · Cited by 78 · see citing works
Enyeart, P. J., Mohr, G., Ellington, A. D., & Lambowitz, A. M. (2014) · Mobile DNA · doi:10.1186/1759-8753-5-2 · Cited by 79 · see citing works
Zhu, L., Dong, H., Zhang, Y., & Li, Y. (2011) · Metabolic Engineering · doi:10.1016/j.ymben.2011.01.009 · Cited by 79 · see citing works
Targeted gene engineering in Clostridium cellulolyticum H10 without methylation
Cui, G.-Z., Hong, W., Zhang, J., Li, W.-L., Feng, Y., Liu, Y.-J., & Cui, Q. (2012) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2012.02.015 · Cited by 77 · see citing works
Application of new metabolic engineering tools for Clostridium acetobutylicum
Lütke‐Eversloh, T. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-5785-5 · Cited by 73 · see citing works
Dai, Z., Dong, H., Zhu, Y., Zhang, Y., Li, Y., & Ma, Y. (2012) · Biotechnology for Biofuels · doi:10.1186/1754-6834-5-44 · Cited by 72 · see citing works
Bruder, M. R., Pyne, M. E., Moo‐Young, M., Chung, D. A., & Chou, C.-H. P. (2016) · Applied and Environmental Microbiology · doi:10.1128/aem.02128-16 · Cited by 73 · see citing works
Chung, D., Farkas, J., & Westpheling, J. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-82 · Cited by 70 · see citing works
Duan, B., Li, M., Sun, Y., Zou, S., & Xu, X. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201801389 · Cited by 69 · see citing works
Ehsaan, M., Kuit, W., Zhang, Y., Cartman, S. T., Heap, J. T., Winzer, K., & Minton, N. P. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0410-0 · Cited by 67 · see citing works
Dong, H., Tao, W., Zhang, Y., & Li, Y. (2012) · Metabolic Engineering · doi:10.1016/j.ymben.2011.10.004 · Cited by 65 · see citing works
Self-citationClosTron-Mediated Engineering of Clostridium
Kuehne, S. A., Heap, J. T., Cooksley, C. M., Cartman, S. T., & Minton, N. P. (2011) · Methods in molecular biology · doi:10.1007/978-1-61779-197-0_23 · Cited by 65 · see citing works
A systematically chromosomally engineered Escherichia coli efficiently produces butanol
Dong, H., Zhao, C., Zhang, T., Zhu, H., Zhao, L., Tao, W., Zhang, Y., & Li, Y. (2017) · Metabolic Engineering · doi:10.1016/j.ymben.2017.10.014 · Cited by 65 · see citing works
Thomas, L., Joseph, A., & Gottumukkala, L. D. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.01.140 · Cited by 63 · see citing works
Ultrasound-Mediated DNA Transformation in Thermophilic Gram-Positive Anaerobes
Lin, L., Song, H., Ji, Y., He, Z., Pu, Y., Zhou, J. Z., & Xu, J. (2010) · PLoS ONE · doi:10.1371/journal.pone.0012582 · Cited by 62 · see citing works
Mao, S., Luo, Y., Bao, G., Zhang, Y., Li, Y., & Ma, Y. (2011) · Molecular BioSystems · doi:10.1039/c0mb00330a · Cited by 52 · see citing works
Systematic engineering of microorganisms to improve alcohol tolerance
Jia, K., Zhang, Y., & Li, Y. (2010) · Engineering in Life Sciences · doi:10.1002/elsc.201000076 · Cited by 48 · see citing works
A functional recT gene for recombineering of Clostridium
Dong, H., Tao, W., Gong, F., Li, Y., & Zhang, Y. (2014) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2013.12.011 · Cited by 45 · see citing works
Biological Production of Butanol and Higher Alcohols
Zhao, J., Lu, C., Chen, C., & Yang, S. (2013) · doi:10.1002/9781118642047.ch13 · Cited by 45 · see citing works
Zuo, F., & Marcotte, H. (2021) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2020.12.015 · Cited by 43 · see citing works
Dai, Z., Dong, H., Zhang, Y., & Li, Y. (2016) · Scientific Reports · doi:10.1038/srep28189 · Cited by 39 · see citing works
Yang, X., Xu, M., & Yang, S. (2015) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-015-7141-9 · Cited by 38 · see citing works
Bioengineering of microorganisms for C3 to C5 alcohols production
Mainguet, S. E., & Liao, J. C. (2010) · Biotechnology Journal · doi:10.1002/biot.201000276 · Cited by 36 · see citing works
Jeamton, W., Dulsawat, S., Tanticharoen, M., Vonshak, A., & Cheevadhanarak, S. (2017) · Plant and Cell Physiology · doi:10.1093/pcp/pcx016 · Cited by 31 · see citing works
Towards New Uses of Botulinum Toxin as a Novel Therapeutic Tool
Pickett, A., & Perrow, K. (2011) · Toxins · doi:10.3390/toxins3010063 · Cited by 33 · see citing works
Metagenomic Analysis Reveals New Microbiota Related to Fiber Digestion in Pigs
Liu, G., Li, P., Hou, L., Niu, Q., Pu, G., Wang, B., Du, T., Kim, S. W., Niu, P., Li, Q., et al. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.746717 · Cited by 32 · see citing works
Luan, G., Dong, H., Zhang, T., Lin, Z., Zhang, Y., Li, Y., & Cai, Z. (2014) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2014.03.010 · Cited by 32 · see citing works
Holt, J. P., Grant, A. J., Coward, C., Maskell, D. J., & Quinlan, J. J. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.01799-12 · Cited by 32 · see citing works
Wang, B., Yu, J., Zhang, W., & Meldrum, D. R. (2015) · Applied and Environmental Microbiology · doi:10.1128/aem.02575-15 · Cited by 32 · see citing works
Pyne, M. E., Moo‐Young, M., Chung, D. A., & Chou, C.-H. P. (2014) · Biotechnology for Biofuels · doi:10.1186/s13068-014-0163-1 · Cited by 29 · see citing works
Zhao, C., Zhao, L., Dong, H., Zhang, Y., & Li, Y. (2017) · Applied and Environmental Microbiology · doi:10.1128/aem.00316-17 · Cited by 29 · see citing works
Group II Intron-Anchored Gene Deletion in Clostridium
Jia, K., Zhu, Y., Zhang, Y., & Li, Y. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016693 · Cited by 29 · see citing works
Jia, K., Zhang, Y., & Li, Y. (2012) · PLoS ONE · doi:10.1371/journal.pone.0038815 · Cited by 28 · see citing works
Cui, G., Zhang, J., Hong, W., Xu, C., Feng, Y., Cui, Q., & Liu, Y. (2013) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-5330-y · Cited by 22 · see citing works
Dai, Z., Zhu, Y., Dong, H., Zhao, C., Zhang, Y., & Li, Y. (2021) · Microbial Cell Factories · doi:10.1186/s12934-021-01639-7 · Cited by 21 · see citing works
Current challenges and advances in butanol production
Patil, R. C., Suryawanshi, P. G., Kataki, R., & Goud, V. V. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-817654-2.00008-3 · Cited by 21 · see citing works
TargeTron Technology Applicable in Solventogenic Clostridia: Revisiting 12 Years’ Advances
Wen, Z., Lu, M., Ledesma‐Amaro, R., Li, Q., Jin, M., & Yang, S. (2019) · Biotechnology Journal · doi:10.1002/biot.201900284 · Cited by 20 · see citing works
Influence of multidrug resistance bacteria in river Ganges in the stretch of Rishikesh to Haridwar
Ali, S., Babali, Singh, S., Singh, R. R., Tyagi, M., & Pandey, R. (2021) · Environmental Challenges · doi:10.1016/j.envc.2021.100068 · Cited by 19 · see citing works
Immunotoxin-mediated targeting of claudin-4 inhibits the proliferation of cancer cells
Hashimi, S. M., Yu, S., Alqurashi, N., Ipe, D. S., & Wei, M.-Q. (2013) · International Journal of Oncology · doi:10.3892/ijo.2013.1881 · Cited by 19 · see citing works
Advances in Biochemical Engineering Biotechnology
Zeng, P.-T., Scheper, T. H., Hu, S., & Kasper, C. (2012) · British Library eBooks · Cited by 19 · see citing works
Huang, C.-N., Liebl, W., & Ehrenreich, A. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1260-3 · Cited by 19 · see citing works
Useh, N. M., & Num, S. M. (2014) · Jordan Journal of Biological Sciences · doi:10.12816/0008220 · Cited by 18 · see citing works
Foulquier, C., Huang, C.-N., Nguyen, N.-P.-T., Thiel, A., Wilding-Steele, T., Soula, J., Yoo, M., Ehrenreich, A., Meynial‐Salles, I., Liebl, W., et al. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1364-4 · Cited by 17 · see citing works
Wu, Q., Zhu, L., Xu, Q. C., Huang, H., Jiang, L., & Yang, S. (2017) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.7b03172 · Cited by 16 · see citing works
Luan, G., Cai, Z., Gong, F., Dong, H., Zhao, L., Zhang, Y., & Li, Y. (2013) · Protein & Cell · doi:10.1007/s13238-013-3079-9 · Cited by 16 · see citing works
Romanowski, S. B., Lee, S. H., Kunakom, S., Paulo, B. S., Recchia, M. J. J., Liu, D. Y., Cavanagh, H., Linington, R. G., & Eustáquio, A. S. (2023) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2304668120 · Cited by 14 · see citing works
Liu, Y., Zhang, J., Cui, G., & Cui, Q. (2015) · Biotechnology Journal · doi:10.1002/biot.201400716 · Cited by 13 · see citing works
Dong, H., Tao, W., Dai, Z., Yang, L., Gong, F., Zhang, Y., & Li, Y. (2011) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2011_128 · Cited by 12 · see citing works
Engineering Cellulase Activity into Clostridium acetobutylicum
Fierobe, H., Mingardon, F., & Chanal, A. (2012) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-415931-0.00016-1 · Cited by 12 · see citing works
Little, G. T., Willson, B. J., Heap, J. T., Winzer, K., & Minton, N. P. (2018) · Biotechnology Journal · doi:10.1002/biot.201700711 · Cited by 11 · see citing works
CAC2634-disrupted mutant of Clostridium acetobutylicum can be electrotransformed in air
Dong, H., Tao, W., Zhu, L., Zhang, Y., & Li, Y. (2011) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2011.03111.x · Cited by 10 · see citing works
A critical review on the development stages of biorefinery systems for sugarcane processing wastes
Wang, D., Sarsaiya, S., Zheng, J., Wong, J. W. C., Zhou, J., & Zhang, J. (2025) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2025.116316 · Cited by 9 · see citing works
Ganguly, J., Martín‐Pascual, M., González, D. M., Bulut, A., Vermeulen, B., Tjalma, I., Vidaki, A., & van Kranenburg, R. (2022) · Microorganisms · doi:10.3390/microorganisms10040698 · Cited by 8 · see citing works
Bao, T., Zhao, J., Zhang, Q., & Yang, S. (2019) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-019-09882-0 · Cited by 8 · see citing works
Tuning of Gene Expression in Clostridium phytofermentans Using Synthetic Promoters and CRISPRi
Rostain, W., Zaplana, T., Boutard, M., Baum, C., Tabuteau, S., Sanitha, M., Ramya, M., Guss, A. M., Ettwiller, L. M., & Tolonen, A. C. (2022) · ACS Synthetic Biology · doi:10.1021/acssynbio.2c00385 · Cited by 7 · see citing works
Butanol as a Drop-In Fuel: A Perspective on Production Methods and Current Status
Ndaba, B., Adeleke, R. A., Makofane, R., Daramola, M. O., & Moshokoa, M. F. (2020) · Green energy and technology · doi:10.1007/978-3-030-38032-8_18 · Cited by 7 · see citing works
New Tools for the Genetic Modification of Industrial Clostridia
Schwarz, K. M., Zhang, Y., Kuit, W., Ehsaan, M., Kovács, K., Winzer, K., & Minton, N. P. (2015) · Elsevier eBooks · doi:10.1016/b978-0-444-59592-8.00013-0 · Cited by 6 · see citing works
Fu, H., Li, X., Guo, X., Yang, D., Nan, C., Cheng, J., Du, H.-C., Shen, M., & Wang, J. (2024) · Bioresource Technology · doi:10.1016/j.biortech.2024.131870 · Cited by 5 · see citing works
Lian, X., Liu, S., Jiang, L., Bai, X., & Wang, Y. (2022) · Biology · doi:10.3390/biology11121724 · Cited by 5 · see citing works
Discovery of a novel gene involved in autolysis ofClostridiumcells
Yang, L., Bao, G., Zhu, Y., Dong, H., Zhang, Y., & Li, Y. (2013) · Protein & Cell · doi:10.1007/s13238-013-3025-x · Cited by 5 · see citing works
Shon, Y. J., Baek, D., Jin, S., Kim, W. J., Jung, G. Y., & Lim, H. G. (2025) · Journal of Biological Engineering · doi:10.1186/s13036-025-00500-4 · Cited by 4 · see citing works
Tao, W., Dong, H., Zhang, Y., Cai, Z., & Li, Y. (2014) · Process Biochemistry · doi:10.1016/j.procbio.2014.10.016 · Cited by 4 · see citing works
Hegazi, A. G., El‐Moez, S. I. A., Abdou, A. M., & Allah, F. M. A. (2014) · Cited by 3 · see citing works
Development of genetic tools for metabolic engineering of Clostridium pasteurianum
Pyne, M. E. (2014) · UWSpace (University of Waterloo) · Cited by 3 · see citing works
Ibrahim, F., Ismail, M., Mohamed, M., Ahmed, M., Zaghloul, M. M., Adawy, Y. A., & El-Rawy, E. M. (2024) · Egyptian Journal of Veterinary Science · doi:10.21608/ejvs.2024.267090.1820 · Cited by 2 · see citing works
[Genetic modification systems for Clostridium acetobutylicum].
Dong, H., Zhang, Y., & Li, Y. (2010) · PubMed · Cited by 2 · see citing works
Development of an efficient host‐vector system of Ruminiclostridium josui
Wang, Y., Okugawa, K., Kunitake, E., Sakka, M., Kimura, T., & Sakka, K. (2018) · Journal of Basic Microbiology · doi:10.1002/jobm.201700620 · Cited by 2 · see citing works
Morente, D. L. (2021) · Repositori digital de la UPF (Universitat Pompeu Fabra) · Cited by 2 · see citing works
Liu, S., Zhang, K., Yu, Y., Lian, X., Jiang, L., Meng, F., Wang, Y., Zhu, X., & Duan, Y. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1283112 · Cited by 1 · see citing works
Salimi, F. (2014) · TSpace (University of Toronto) · Cited by 1 · see citing works
Creation of Novel Methylating Conjugal Donor Strains for Genetically Recalcitrant BacteriaPreprint
Dempster, A. W., Mansfield, R. T., Ingle, P., Bean, Z., Akaluka, C., Millard, J., & Minton, N. P. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.01.22.701203 · Cited by 1 · see citing works
Nale, J. Y. (2013) · Figshare · Cited by 1 · see citing works
Characterisation and functional analysis of the putative agr system in Clostridium acetobutylicum
Scott, J. (2012) · UPT. Syiah Kuala University Library (Syiah Kuala University)
Jensen, T. Ø., Kvist, T., Mikkelsen, M. J., & Westermann, P. (2014) · Letters in Applied Microbiology · doi:10.1111/lam.12238
Discovery of a novel gene involved in autolysis of Clostridium cells ESEARCH ARTICLE R
Yang, L., Bao, G., Zhu, Y., Dong, H., Zhang, Y., & Li, Y. (2013)
Dai, Z., Zhu, Y., Dong, H., Zhao, C., Zhang, Y., & Li, Y. (2021) · Figshare · doi:10.6084/m9.figshare.15079523
Use of Montanide gel as an adjuvant for poly valent clostridial vaccine
Adawy, Y. A., Adel, E. H. H. A., El-Sergany, E., Khalaf, N. M., Ahmed, M., & Mohamed, A. A. E. B. (2025) · Egyptian Journal of Veterinary Science · doi:10.21608/ejvs.2025.382723.2845
Xiao, C., Dou, J., Zhang, N., Liu, L., Du, S., Rao, X., & Yu, L. (2025) · Fermentation · doi:10.3390/fermentation11090526
Dai, Z., Zhu, Y., Dong, H., Zhao, C., Zhang, Y., & Li, Y. (2021) · Figshare · doi:10.6084/m9.figshare.15079520
- Authors
- Dong, H., Zhang, Y., Dai, Z., & Li, Y.
- Affiliations
- Chinese Academy of Sciences (CN)
- University of Science and Technology of China (CN)
- Corresponding author
- Li, Yin
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2010-02-09
- Volume
- 5
- Issue
- 2
- Pages
- e9038
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC2817722
- OpenAlex
- W2031351113
- Cited by
- 91
- References
- 51
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 6 · 2020: 1 · 2019: 6 · 2018: 4 · 2017: 4 · 2016: 6 · 2015: 4 · 2014: 13 · 2013: 9 · 2012: 10
- FWCI
- 6.29
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Chinese Academy of Sciences
- National Key Research and Development Program of China (2007CB707803, 973 Project)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Clostridium acetobutylicum, restriction-modification systems, type II restriction endonuclease, DNA methylation, genome editing
- MeSH terms
- Bacterial Proteins, Clostridium, DNA, Genetic Engineering, Mutation, Plasmids, Transformation, Genetic, Deoxyribonucleases, Type II Site-Specific, DNA Restriction-Modification Enzymes, DNA Methylation, Clostridium acetobutylicum
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review