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Cited by 237

The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.

In vivo imaging and tracking of host–microbiota interactions via metabolic labeling of gut anaerobic bacteria

Open access
Geva-Zatorsky, Naama, Alvarez, David, Hudak, Jason E, Reading, Nicola C, Erturk-Hasdemir, Deniz, Dasgupta, Suryasarathi, von Andrian, Ulrich H, Kasper, Dennis L·2015·Nature Medicine
DOI 10.1038/nm.3929
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Abstract

The intestine is densely populated by anaerobic commensal bacteria. These microorganisms shape immune system development, but understanding of host–commensal interactions is hampered by a lack of tools for studying the anaerobic intestinal environment. We applied metabolic oligosaccharide engineering and bioorthogonal click chemistry to label various commensal anaerobes, including Bacteroides fragilis, a common and immunologically important commensal. We studied the dissemination of B. fragilis after acute peritonitis and characterized the interactions of the intact microbe and its polysaccharide components in myeloid and B cell lineages. We were able to assess the distribution and colonization of labeled B. fragilis along the intestine, as well as niche competition after coadministration of multiple species of the microbiota. We also fluorescently labeled nine additional commensals (eight anaerobic and one microaerophilic) from three phyla common in the gut—Bacteroidetes, Firmicutes and Proteobacteria—as well as one aerobic pathogen (Staphylococcus aureus). This strategy permits visualization of the anaerobic microbial niche by various methods, including intravital two-photon microscopy and non-invasive whole-body imaging, and can be used to study microbial colonization and host–microbe interactions in real time.

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Research works printed but not linked: 27

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Cited by

Metadata

Authors
Geva-Zatorsky, N., Alvarez, D., Hudak, J. E., Reading, N. C., Erturk-Hasdemir, D., Dasgupta, S., von Andrian, U. H., & Kasper, D. L.
Venue
Nature Medicine
Publisher
Springer Science and Business Media LLC
Published
2015-08-17
Volume
21
Issue
9
Pages
1091-1100
ISSN
  • 1078-8956 (ISSN-L)
  • 1546-170X
DOI
10.1038/nm.3929
OpenAlex
W1852244784
Cited by
237
References
10
FWCI
7.33
Type
journal article
Language
en
Open access
green
Licence

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