Harnessing nanotopography and integrin–matrix interactions to influence stem cell fate
Abstract
Stem cells respond to nanoscale surface features, with changes in cell growth and differentiation mediated by alterations in cell adhesion. The interaction of nanotopographical features with integrin receptors in the cells' focal adhesions alters how the cells adhere to materials surfaces, and defines cell fate through changes in both cell biochemistry and cell morphology. In this Review, we discuss how cell adhesions interact with nanotopography, and we provide insight as to how materials scientists can exploit these interactions to direct stem cell fate and to understand how the behaviour of stem cells in their niche can be controlled. We expect knowledge gained from the study of cell-nanotopography interactions to accelerate the development of next-generation stem cell culture materials and implant interfaces, and to fuel discovery of stem cell therapeutics to support regenerative therapies.
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Polymers can be conjugated with heparin to bind growth factors, or growth factors can be conjugated to the polymer itself, to mimic ECM binding.
“researchers conjugated polymers with heparin which can bind to growth factors, or conjugated these growth factors to the polymer itself89.”
From Organoids (2022) · cites this paper as [89]
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Investigation of the limits of nanoscale filopodial interactions
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A genomics approach in determining nanotopographical effects on MSC phenotype
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Zhao, Z., Chen, X., Dowbaj, A. M., Sljukic, A., Bratlie, K., Lin, L., Fong, E. L. S., Balachander, G. M., Chen, Z., Soragni, A., et al. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00174-y · Cited by 1,001 · see citing works
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Hurdles to uptake of mesenchymal stem cells and their progenitors in therapeutic products
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Self-citation
- Authors
- Dalby, M. J., Gadegaard, N., & Oreffo, R. O. C.
- Affiliations
- University of Glasgow (GB)
- University of Southampton (GB)
- Corresponding author
- Dalby, Matthew J.
- Venue
- Nature Materials
- Published
- 2014-05-21
- Volume
- 13
- Issue
- 6
- Pages
- 558-569
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W2046576188
- Cited by
- 1,082
- References
- 117
- Citations by year
- 2026: 48 · 2025: 80 · 2024: 55 · 2023: 74 · 2022: 87 · 2021: 105 · 2020: 110 · 2019: 83 · 2018: 108 · 2017: 125 · 2016: 97 · 2015: 86 · 2014: 24
- FWCI
- 11.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Materials (publishedVersion)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- ePrints Soton (University of Southampton) (submittedVersion)
- Funders
- Engineering and Physical Sciences Research Council (EP/K034898/1, EP/G048703/1)
- Biotechnology and Biological Sciences Research Council (BB/G008868/1, BB/K011235/1, JF20604, BB/E012256/1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- stem cell fate, nanotopography, cell adhesion, focal adhesions, cell differentiation, extracellular matrix, cell-substrate interaction, stem cell niche, regenerative medicine
- MeSH terms
- Cell Adhesion, Extracellular Matrix, Humans, Stem Cells, Integrins, Focal Adhesions
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