Small functional groups for controlled differentiation of hydrogel-encapsulated human mesenchymal stem cells
Open accessAbstract
Cell-matrix interactions have critical roles in regeneration, development and disease. The work presented here demonstrates that encapsulated human mesenchymal stem cells (hMSCs) can be induced to differentiate down osteogenic and adipogenic pathways by controlling their three-dimensional environment using tethered small-molecule chemical functional groups. Hydrogels were formed using sufficiently low concentrations of tether molecules to maintain constant physical characteristics, encapsulation of hMSCs in three dimensions prevented changes in cell morphology, and hMSCs were shown to differentiate in normal growth media, indicating that the small-molecule functional groups induced differentiation. To our knowledge, this is the first example where synthetic matrices are shown to control induction of multiple hMSC lineages purely through interactions with small-molecule chemical functional groups tethered to the hydrogel material. Strategies using simple chemistry to control complex biological processes would be particularly powerful as they could make production of therapeutic materials simpler, cheaper and more easily controlled.
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3D scaffolds and associated cell-encapsulation techniques provide valuable tools for deciphering how ECM affects fate of cells.
“These 3D scaffolds and associated cell-encapsulation techniques provide the valuable tools for helping us decipher how the ECM affects the fate of cells (5, 13, 14, 25-27, 68, 77, 80, 87, 88, 92, 108).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [5]
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- Authors
- Benoit, D. S. W., Schwartz, M. P., Durney, A. R., & Anseth, K. S.
- Affiliations
- University of Colorado Boulder (US)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Anseth, Kristi S.
- Venue
- Nature Materials
- Published
- 2008-08-24
- Volume
- 7
- Issue
- 10
- Pages
- 816-823
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- PMC
- PMC2929915
- OpenAlex
- W2130606072
- Cited by
- 825
- References
- 41
- Citations by year
- 2026: 10 · 2025: 23 · 2024: 24 · 2023: 19 · 2022: 29 · 2021: 34 · 2020: 43 · 2019: 46 · 2018: 74 · 2017: 65 · 2016: 80 · 2015: 61 · 2014: 55 · 2013: 55 · 2012: 81
- FWCI
- 20.51
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Materials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute of Dental and Craniofacial Research (DE016523, R01DE016523)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- human mesenchymal stem cells, hydrogel encapsulation, cell-matrix interactions, osteogenic differentiation, adipogenesis, 3D cell culture, cell differentiation control
- MeSH terms
- Cell Differentiation, Cells, Cultured, Extracellular Matrix, Humans, Materials Testing, Osteogenesis, Polyethylene Glycols, Gene Expression, Coated Materials, Biocompatible, Hydrogels, Tissue Engineering, Adipogenesis, Mesenchymal Stem Cells
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