The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
A photoreversible protein-patterning approach for guiding stem cell fate in three-dimensional gels
Abstract
Although biochemically patterned hydrogels are capable of recapitulating many critical aspects of the heterogeneous cellular niche, exercising spatial and temporal control of the presentation and removal of biomolecular signalling cues in such systems has proved difficult. Here, we demonstrate a synthetic strategy that exploits two bioorthogonal photochemistries to achieve reversible immobilization of bioactive full-length proteins with good spatial and temporal control within synthetic, cell-laden biomimetic scaffolds. A photodeprotection-oxime-ligation sequence permits user-defined quantities of proteins to be anchored within distinct subvolumes of a three-dimensional matrix, and an ortho-nitrobenzyl ester photoscission reaction facilitates subsequent protein removal. By using this approach to pattern the presentation of the extracellular matrix protein vitronectin, we accomplished reversible differentiation of human mesenchymal stem cells to osteoblasts in a spatially defined manner. Our protein-patterning approach should provide further avenues to probe and direct changes in cell physiology in response to dynamic biochemical signalling.
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“Synthetic hydrogels can be functionalized with biologically active moieties that permit the growth and spread of cells146–151; concentration and spacing of these cues can be changed independently152–155.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [155]
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Cites
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Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties
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Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate
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Advances in engineering hydrogels
Zhang, Y. S., & Khademhosseini, A. (2017) · Science · doi:10.1126/science.aaf3627 · Cited by 2,908
“[Adapted with permission from (75), copyright 2015 Nature Publishing Group] (C) Cell-responsive cleavage and anticleavage negative feedback system.”
BackgroundMethodA practical guide to hydrogels for cell culture
Caliari, S. R., & Burdick, J. A. (2016) · Nature Methods · doi:10.1038/nmeth.3839 · Cited by 2,075
“This also includes spatial patterning, where numerous biochemical and biophysical signals can be introduced in a heterogeneous manner to control populations of single cell types or co-cultures 92,93 .”
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Cao, H., Duan, L., Zhang, Y., Cao, J., & Zhang, K. (2021) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-021-00830-x · Cited by 1,380
“Especially, the photodeprotection-oximeligation sequence and ortho-nitrobenzyl ester photo-cleavage reaction within the hydrogel could bring about protein anchorage and removal, respectively.(322) Besides chemical cues, the biomechanical properties could also be modulated dynamically for manipulating cell behaviors.”
BackgroundIn situ forming injectable hydrogels for drug delivery and wound repair
Dimatteo, R., Darling, N. J., & Segura, T. (2018) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2018.03.007 · Cited by 924
“(Source: Adapted with permissions from Refs [103,104,107,110].”
Metadata
- Authors
- DeForest, C. A., & Tirrell, D. A.
- Affiliations
- California Institute of Technology (US)
- Corresponding author
- DeForest, Cole A.
- Tirrell, David A.
- Venue
- Nature Materials
- Published
- 2015-02-23
- Volume
- 14
- Issue
- 5
- Pages
- 523-531
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W2088868630
- Cited by
- 432
- References
- 55
- Citations by year
- 2026: 14 · 2025: 22 · 2024: 23 · 2023: 33 · 2022: 21 · 2021: 52 · 2020: 55 · 2019: 49 · 2018: 68 · 2017: 54 · 2016: 33 · 2015: 8
- FWCI
- 22.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Materials (publishedVersion)
- PubMed (publishedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- protein immobilization, three-dimensional hydrogels, cell-laden scaffolds, vitronectin, mesenchymal stem cells, osteogenic differentiation, biomimetic scaffolds
- MeSH terms
- Cell Differentiation, Humans, Osteoblasts, Vitronectin, Hydrogels, Biomimetic Materials, Tissue Scaffolds, Photochemical Processes, Immobilized Proteins, Mesenchymal Stem Cells
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