The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Matrix elasticity of void-forming hydrogels controls transplanted-stem-cell-mediated bone formation
Open accessAbstract
The effectiveness of stem-cell therapies has been hampered by cell death and limited control over fate1. These problems can be partially circumvented by using macroporous biomaterials that improve the survival of transplanted stem cells and provide molecular cues to direct cell phenotype2–4. Stem cell behavior can also be controlled in vitro by manipulating the elasticity of both porous and non-porous materials5–7, yet translation to therapeutic processes in vivo remains elusive. Here, by developing injectable, void-forming hydrogels that decouple pore formation from elasticity, we show that mesenchymal stem cell (MSC) osteogenesis in vitro, and cell deployment in vitro and in vivo, can be controlled by modifying, respectively, the hydrogel's elastic modulus or its chemistry. When the hydrogels were used to transplant MSCs, the hydrogel's elasticity regulated bone regeneration, with optimal bone formation at 60 kPa. Our findings show that biophysical cues can be harnessed to direct therapeutic stem-cell behaviors in situ.
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Cites
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Self-citationThe stem-cell niche as an entity of action
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Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture
Gilbert, P. M., Havenstrite, K. L., Magnusson, K. E. G., Sacco, A., Leonardi, N. A., Kraft, P., Nguyen, N. K., Thrun, S., Lutolf, M. P., & Blau, H. M. (2010) · Science · doi:10.1126/science.1191035 · Cited by 1,587
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Self-citationExtracellular-matrix tethering regulates stem-cell fate
Trappmann, B., Gautrot, J. E., Connelly, J. T., Strange, D. G. T., Li, Y., Oyen, M. L., Cohen Stuart, M. A., Boehm, H., Li, B., Vogel, V., et al. (2012) · Nature Materials · doi:10.1038/nmat3339 · Cited by 1,568
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Cited by
Designing hydrogels for controlled drug delivery
Li, J., & Mooney, D. J. (2016) · Nature Reviews Materials · doi:10.1038/natrevmats.2016.71 · Cited by 4,832
Self-citationEffects of extracellular matrix viscoelasticity on cellular behaviour
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Self-citationMechanical forces direct stem cell behaviour in development and regeneration
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Self-citationBioactive hydrogels for bone regeneration
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Metadata
- Authors
- Huebsch, N., Lippens, E., Lee, K., Mehta, M., Koshy, S. T., Darnell, M. C., Desai, R. M., Madl, C. M., Xu, M., Zhao, X., Chaudhuri, O., Verbeke, C., Kim, W. S., Alim, K., Mammoto, A., Ingber, D. E., Duda, G. N., & Mooney, D. J.
- Venue
- Nature Materials
- Published
- 2015-09-14
- Volume
- 14
- Issue
- 12
- Pages
- 1269-1277
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W2206958686
- Cited by
- 521
- References
- 47
- FWCI
- 21.07
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Huebsch, N., Lippens, E., Lee, K., Mehta, M., Koshy, S. T., Darnell, M. C., Desai, R. M., Madl, C. M., Xu, M., Zhao, X., Chaudhuri, O., Verbeke, C., Kim, W. S., Alim, K., Mammoto, A., Ingber, D. E., Duda, G. N., & Mooney, D. J. (2015). Matrix elasticity of void-forming hydrogels controls transplanted-stem-cell-mediated bone formation. Nature Materials. https://doi.org/10.1038/nmat4407
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