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A large genome center's improvements to the Illumina sequencing system
Open accessAbstract
The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.
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Metadata
- Authors
- Quail, M. A., Kozarewa, I., Smith, F., Scally, A., Stephens, P. J., Durbin, R., Swerdlow, H., & Turner, D. J.
- Venue
- Nature Methods
- Published
- 2008-11-25
- Volume
- 5
- Issue
- 12
- Pages
- 1005-1010
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2039716761
- Cited by
- 727
- References
- 1
- FWCI
- 20.35
- Type
- journal article
- Language
- en
- Open access
- green
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