The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Cas9 gRNA engineering for genome editing, activation and repression
Open accessAbstract
We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
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Metadata
- Authors
- Kiani, S., Chavez, A., Tuttle, M., Hall, R. N., Chari, R., Ter-Ovanesyan, D., Qian, J., Pruitt, B. W., Beal, J., Vora, S., Buchthal, J., Kowal, E. J. K., Ebrahimkhani, M. R., Collins, J. J., Weiss, R., & Church, G.
- Venue
- Nature Methods
- Published
- 2015-09-07
- Volume
- 12
- Issue
- 11
- Pages
- 1051-1054
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W1751510803
- Cited by
- 334
- References
- 8
- FWCI
- 14.41
- Type
- journal article
- Language
- en
- Open access
- green
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