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Cited by 213

The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.

Potential pitfalls of CRISPR/Cas9‐mediated genome editing

Peng, Rongxue, Lin, Guigao, Li, Jinming·2016·The FEBS Journal
DOI 10.1111/febs.13586
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Abstract

Recently, a novel technique named the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated protein (Cas)9 system has been rapidly developed. This genome editing tool has improved our ability tremendously with respect to exploring the pathogenesis of diseases and correcting disease mutations, as well as phenotypes. With a short guide RNA, Cas9 can be precisely directed to target sites, and functions as an endonuclease to efficiently produce breaks in DNA double strands. Over the past 30 years, CRISPR has evolved from the ‘curious sequences of unknown biological function’ into a promising genome editing tool. As a result of the incessant development in the CRISPR/Cas9 system, Cas9 co‐expressed with custom guide RNA s has been successfully used in a variety of cells and organisms. This genome editing technology can also be applied to synthetic biology, functional genomic screening, transcriptional modulation and gene therapy. However, although CRISPR/Cas9 has a broad range of action in science, there are several aspects that affect its efficiency and specificity, including Cas9 activity, target site selection and short guide RNA design, delivery methods, off‐target effects and the incidence of homology‐directed repair. In the present review, we highlight the factors that affect the utilization of CRISPR/Cas9, as well as possible strategies for handling any problems. Addressing these issues will allow us to take better advantage of this technique. In addition, we also review the history and rapid development of the CRISPR/Cas system from the time of its initial discovery in 2012.

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Metadata

Authors
Peng, R., Lin, G., & Li, J.
Affiliations
National Center for Clinical Laboratories Beijing Hospital Beijing China
Venue
The FEBS Journal
Publisher
Wiley
Published
2015-11-27
Volume
283
Issue
7
Pages
1218-1231
ISSN
  • 1742-464X
  • 1742-4658
DOI
10.1111/febs.13586
Cited by
213
References
21
Language
en
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Funders
  • Beijing Municipal Public Health Bureau (2011-4011-02)
  • National Population and Family Planning Commission of the People's Republic of China (201402018)

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