The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
In vivo quantification of spatially varying mechanical properties in developing tissues
Open accessAbstract
The mechanical properties of the cellular microenvironment and their spatiotemporal variations are thought to play a central role in sculpting embryonic tissues, maintaining organ architecture and controlling cell behavior, including cell differentiation. However, no direct in vivo and in situ measurement of mechanical properties within developing 3D tissues and organs has yet been performed. Here we introduce a technique that employs biocompatible, magnetically responsive ferrofluid microdroplets as local mechanical actuators and allows quantitative spatiotemporal measurements of mechanical properties in vivo. Using this technique, we show that vertebrate body elongation entails spatially varying tissue mechanics along the anteroposterior axis. Specifically, we find that the zebrafish tailbud is viscoelastic (elastic below a few seconds and fluid after just 1 min) and displays decreasing stiffness and increasing fluidity toward its posterior elongating region. This method opens new avenues to study mechanobiology in vivo, both in embryogenesis and in disease processes, including cancer.
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Cites
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Tensional homeostasis and the malignant phenotype
Paszek, M. J., Zahir, N., Johnson, K. R., Lakins, J. N., Rozenberg, G. I., Gefen, A., Reinhart-King, C. A., Margulies, S. S., Dembo, M., Boettiger, D., et al. (2005) · Cancer Cell · doi:10.1016/j.ccr.2005.08.010 · Cited by 3,941
Hydrogels with tunable stress relaxation regulate stem cell fate and activity
Chaudhuri, O., Gu, L., Klumpers, D., Darnell, M., Bencherif, S. A., Weaver, J. C., Huebsch, N., Lee, H.-P., Lippens, E., Duda, G. N., et al. (2016) · Nature Materials · doi:10.1038/nmat4489 · Cited by 2,443
Balaban, N. Q., Schwarz, U. S., Riveline, D., Goichberg, P., Tzur, G., Sabanay, I., Mahalu, D., Safran, S., Bershadsky, A., Addadi, L., et al. (2001) · Nature Cell Biology · doi:10.1038/35074532 · Cited by 2,132
Control of Stem Cell Fate by Physical Interactions with the Extracellular Matrix
Guilak, F., Cohen, D. M., Estes, B. T., Gimble, J. M., Liedtke, W., & Chen, C. S. (2009) · Cell Stem Cell · doi:10.1016/j.stem.2009.06.016 · Cited by 1,850
Forces in Tissue Morphogenesis and Patterning
Heisenberg, C.-P., & Bellaïche, Y. (2013) · Cell · doi:10.1016/j.cell.2013.05.008 · Cited by 1,216
Zaman, M. H., Trapani, L. M., Sieminski, A. L., MacKellar, D., Gong, H., Kamm, R. D., Wells, A., Lauffenburger, D. A., & Matsudaira, P. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604460103 · Cited by 1,160
Nelson, C. M., & Bissell, M. J. (2006) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.22.010305.104315 · Cited by 1,130
N., E., & Thompson, D. W. (1945) · The Journal of Philosophy · doi:10.2307/2019330 · Cited by 1,119
Wei, S. C., Fattet, L., Tsai, J. H., Guo, Y., Pai, V. H., Majeski, H. E., Chen, A. C., Sah, R. L., Taylor, S. S., Engler, A. J., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3157 · Cited by 948
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The stiffness of living tissues and its implications for tissue engineering
Guimarães, C. F., Gasperini, L., Marques, A. P., & Reis, R. L. (2020) · Nature Reviews Materials · doi:10.1038/s41578-019-0169-1 · Cited by 1,515
Role of Extracellular Matrix in Development and Cancer Progression
Walker, C., Mojares, E., & Del Río Hernández, A. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19103028 · Cited by 1,084
Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
Huang, G., Li, F., Zhao, X., Ma, Y., Li, Y., Lin, M., Jin, G., Lu, T. J., Genin, G. M., & Xu, F. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00094 · Cited by 825
Viscoelastic hydrogels for 3D cell culture
Chaudhuri, O. (2017) · Biomaterials Science · doi:10.1039/c7bm00261k · Cited by 353
Mechanotransduction in tumor progression: The dark side of the force
Broders-Bondon, F., Nguyen Ho-Bouldoires, T. H., Fernandez-Sanchez, M.-E., & Farge, E. (2018) · Journal of Cell Biology · doi:10.1083/jcb.201701039 · Cited by 319
Adherens junctions as molecular regulators of emergent tissue mechanics
Campàs, O., Noordstra, I., & Yap, A. S. (2024) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-023-00688-7 · Cited by 149
Self-citationSculpting tissues by phase transitions
Lenne, P.-F., & Trivedi, V. (2022) · Nature Communications · doi:10.1038/s41467-022-28151-9 · Cited by 110
Viscoelastic Networks: Forming Cells and Tissues
Corominas-Murtra, B., & Petridou, N. I. (2021) · Frontiers in Physics · doi:10.3389/fphy.2021.666916 · Cited by 69
Metadata
- Authors
- Serwane, F., Mongera, A., Rowghanian, P., Kealhofer, D. A., Lucio, A. A., Hockenbery, Z. M., & Campàs, O.
- Venue
- Nature Methods
- Published
- 2016-12-05
- Volume
- 14
- Issue
- 2
- Pages
- 181-186
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2560819934
- Cited by
- 367
- References
- 53
- FWCI
- 15.06
- Type
- journal article
- Language
- en
- Open access
- green
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