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Cited by 367

The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.

In vivo quantification of spatially varying mechanical properties in developing tissues

Open access
Serwane, Friedhelm, Mongera, Alessandro, Rowghanian, Payam, Kealhofer, David A, Lucio, Adam A, Hockenbery, Zachary M, Campàs, Otger·2017·Nature Methods
DOI 10.1038/nmeth.4101
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Abstract

The mechanical properties of the cellular microenvironment and their spatiotemporal variations are thought to play a central role in sculpting embryonic tissues, maintaining organ architecture and controlling cell behavior, including cell differentiation. However, no direct in vivo and in situ measurement of mechanical properties within developing 3D tissues and organs has yet been performed. Here we introduce a technique that employs biocompatible, magnetically responsive ferrofluid microdroplets as local mechanical actuators and allows quantitative spatiotemporal measurements of mechanical properties in vivo. Using this technique, we show that vertebrate body elongation entails spatially varying tissue mechanics along the anteroposterior axis. Specifically, we find that the zebrafish tailbud is viscoelastic (elastic below a few seconds and fluid after just 1 min) and displays decreasing stiffness and increasing fluidity toward its posterior elongating region. This method opens new avenues to study mechanobiology in vivo, both in embryogenesis and in disease processes, including cancer.

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Metadata

Authors
Serwane, F., Mongera, A., Rowghanian, P., Kealhofer, D. A., Lucio, A. A., Hockenbery, Z. M., & Campàs, O.
Venue
Nature Methods
Publisher
Springer Science and Business Media LLC
Published
2016-12-05
Volume
14
Issue
2
Pages
181-186
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
DOI
10.1038/nmeth.4101
OpenAlex
W2560819934
Cited by
367
References
53
FWCI
15.06
Type
journal article
Language
en
Open access
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