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Cited by 260

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TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding

Open access
Schofield, Jeremy A, Duffy, Erin E, Kiefer, Lea, Sullivan, Meaghan C, Simon, Matthew D·2018·Nature Methods
DOI 10.1038/nmeth.4582
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Abstract

RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single-molecule approach that is adaptable to many applications and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq.

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Citations

Cites

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Cited by

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Metadata

Authors
Schofield, J. A., Duffy, E. E., Kiefer, L., Sullivan, M. C., & Simon, M. D.
Venue
Nature Methods
Publisher
Springer Science and Business Media LLC
Published
2018-01-22
Volume
15
Issue
3
Pages
221-225
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
DOI
10.1038/nmeth.4582
OpenAlex
W2784500348
Cited by
260
References
9
FWCI
7.93
Type
journal article
Language
en
Open access
green
Licence
www.springer.com/tdm (tdm)

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