Reorganization of cortical population activity imaged throughout long-term sensory deprivation
Open accessAbstract
Sensory maps are reshaped by experience. It is unknown how map plasticity occurs in vivo in functionally diverse neuronal populations because activity of the same cells has not been tracked over long time periods. Here we used repeated two-photon imaging of a genetic calcium indicator to measure whisker-evoked responsiveness of the same layer 2/3 neurons in adult mouse barrel cortex over weeks, first with whiskers intact, then during continued trimming of all but one whisker. Across the baseline period, neurons displayed heterogeneous yet stable responsiveness. During sensory deprivation, responses to trimmed whisker stimulation globally decreased, whereas responses to spared whisker stimulation increased for the least active neurons and decreased for the most active neurons. These findings suggest that recruitment of inactive, 'silent' neurons is part of a convergent redistribution of population activity underlying sensory map plasticity. Sensory-driven responsiveness is a key property controlling experience-dependent activity changes in individual neurons.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The difference in D2W map size was most noticeable at +21 d.
“This difference was most noticeable at +21 d, which coincides with the time when previous studies have detected the largest effect of whisker deprivation on OIS map size (Margolis et al., 2012).”
From The E3 ubiquitin ligase IDOL regulates synaptic ApoER2 levels and is important for plasticity and learning (2017) · cites this paper as Margolis et al., 2012
Cites
CORTICAL PLASTICITY: From Synapses to Maps
Buonomano, D. V., & Merzenich, M. M. (1998) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.21.1.149 · Cited by 2,083 · see citing works
The excitatory neuronal network of the C2 barrel column in mouse primary somatosensory cortex
Lefort, S., Tomm, C., Floyd Sarria, J.-C., & Petersen, C. C. H. (2009) · Neuron · doi:10.1016/j.neuron.2008.12.020 · Cited by 875 · see citing works
An embedded subnetwork of highly active neurons in the neocortex
Yassin, L., Benedetti, B. L., Jouhanneau, J.-S., Wen, J. A., Poulet, J. F. A., & Barth, A. L. (2010) · Neuron · doi:10.1016/j.neuron.2010.11.029 · Cited by 227 · see citing works
Bidirectional plasticity in fast-spiking GABA circuits by visual experience
Yazaki-Sugiyama, Y., Kang, S., Câteau, H., Fukai, T., & Hensch, T. K. (2009) · Nature · doi:10.1038/nature08485 · Cited by 200 · see citing works
Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor
Wallace, D. J., zum Alten Borgloh, S. M., Astori, S., Yang, Y., Bausen, M., Kügler, S., Palmer, A. E., Tsien, R. Y., Sprengel, R., Kerr, J. N. D., et al. (2008) · Nature Methods · doi:10.1038/nmeth.1242 · Cited by 191 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo
Dana, H., Chen, T.-W., Hu, A., Shields, B. C., Guo, C., Looger, L. L., Kim, D. S., & Svoboda, K. (2014) · PLoS ONE · doi:10.1371/journal.pone.0108697 · Cited by 574 · see citing works
Dynamic Reorganization of Neuronal Activity Patterns in Parietal Cortex
Driscoll, L. N., Pettit, N. L., Minderer, M., Chettih, S. N., & Harvey, C. D. (2017) · Cell · doi:10.1016/j.cell.2017.07.021 · Cited by 445 · see citing works
Astrocyte Ca2+ signalling: an unexpected complexity
Volterra, A., Liaudet, N., & Savtchouk, I. (2014) · Nature Reviews Neuroscience · doi:10.1038/nrn3725 · Cited by 446 · see citing works
Feldmeyer, D., Brecht, M., Helmchen, F., Petersen, C. C. H., Poulet, J. F. A., Staiger, J. F., Luhmann, H. J., & Schwarz, C. (2013) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2012.11.002 · Cited by 354 · see citing works
“Using viral approaches these protein indicators can be stably expressed in confined neuronal populations over weeks or even months (Margolis et al., 2012; O’Connor et al., 2010b).”
BackgroundSelf-citationCortical Circuit Activity Evokes Rapid Astrocyte Calcium Signals on a Similar Timescale to Neurons
Stobart, J. L., Ferrari, K. D., Barrett, M. J. P., Glück, C., Stobart, M. J., Zuend, M., & Weber, B. (2018) · Neuron · doi:10.1016/j.neuron.2018.03.050 · Cited by 254 · see citing works
Self-citationHow microglia sense and regulate neuronal activity
Umpierre, A. D., & Wu, L. (2021) · Glia · doi:10.1002/glia.23961 · Cited by 237 · see citing works
Dense functional and molecular readout of a circuit hub in sensory cortex
Condylis, C., Ghanbari, A., Manjrekar, N., Bistrong, K., Yao, S., Yao, Z., Nguyen, T. N., Zeng, H., Tasic, B., & Chen, J. L. (2022) · Science · doi:10.1126/science.abl5981 · Cited by 81 · see citing works
Neocortical dynamics during whisker-based sensory discrimination in head-restrained mice
Helmchen, F., Gilad, A., & Chen, J. L. (2018) · Neuroscience · doi:10.1016/j.neuroscience.2017.09.003 · Cited by 38 · see citing works
“Here, the ability to consistently find the same cells under the two-photon microscope again and again over days to weeks – using anatomical landmarks such as blood vessel pattern and neuronal cell body constellations (Margolis et al., 2012) – is a crucial advantage.”
Self-citationGao, J., Marosi, M., Choi, J., Achiro, J. M., Kim, S., Li, S., Otis, K., Martin, K. C., Portera-Cailliau, C., & Tontonoz, P. (2017) · eLife · doi:10.7554/elife.29178 · Cited by 30 · see citing works
- Authors
- Margolis, D. J., Lütcke, H., Schulz, K., Haiss, F., Weber, B., Kügler, S., Hasan, M. T., & Helmchen, F.
- Affiliations
- University of Zurich (CH)
- Universitätsmedizin Göttingen (DE)
- Max Planck Institute for Medical Research (DE)
- Corresponding author
- Helmchen, Fritjof
- ORCID
- Venue
- Nature Neuroscience
- Published
- 2012-10-21
- Volume
- 15
- Issue
- 11
- Pages
- 1539-1546
- ISSN
- 1097-6256 (ISSN-L)
- 1546-1726
- OpenAlex
- W2029261862
- Cited by
- 203
- References
- 5
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 17 · 2023: 10 · 2022: 17 · 2021: 14 · 2020: 16 · 2019: 12 · 2018: 16 · 2017: 12 · 2016: 13 · 2015: 18 · 2014: 17 · 2013: 24 · 2012: 1
- FWCI
- 9.19
- Type
- journal article
- Language
- en
- Open access
- green
- zenodo.org
- Licence
- other-oa
- www.springer.com/tdm (tdm)
- Locations
- Nature Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Zurich Open Repository and Archive (University of Zurich) (acceptedVersion)
- GRO (submittedVersion)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion)
- Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, other-oa)
- Zurich Open Repository and Archive (University of Zurich)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Tactile and Sensory Interactions
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- sensory deprivation, barrel cortex, whisker trimming, population activity, layer 2/3 neurons, experience-dependent plasticity, silent neurons, neural recruitment, sensory maps, two-photon calcium imaging
- MeSH terms
- Action Potentials, Animals, Brain Mapping, Calcium, Cerebral Cortex, Computer Simulation, Female, Gene Expression Regulation, Luminescent Proteins, Male, Mice, Inbred C57BL, Models, Biological, Neurons, Physical Stimulation, Sensory Deprivation, Time Factors, Transduction, Genetic, Vibrissae, Synapsins, Neuropil, Mice, Optics and Photonics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
