Cited by 781Neuroinflammation and Neurodegeneration MechanismsNeurogenesis and neuroplasticity mechanismsNeuroscience and Neuropharmacology Research

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Layer V cortical neurons require microglial support for survival during postnatal development

Ueno, Masaki, Fujita, Yuki, Tanaka, Tatsuhide, Nakamura, Yuka, Kikuta, Junichi, Ishii, Masaru, Yamashita, Toshihide·2013·Nature Neuroscience

DOI 10.1038/nn.3358

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Abstract

Neurons require trophic support during neural circuit formation; however, how the cellular milieu contributes to neuronal survival remains unclear. We found that layer V cortical neurons require support from microglia for survival during postnatal development. Specifically, we found that microglia accumulated close to the subcerebral and callosal projection axons in the postnatal brain. Inactivation of microglia by minocycline treatment or transient ablation of microglia in CD11b-DTR transgenic mice led to increased apoptosis, specifically in layer V subcerebral and callosal projection neurons. CX3CR1 in microglia was required for the survival of layer V neurons. Microglia consistently promoted the survival of cortical neurons in vitro. In addition, we identified microglia-derived IGF1 as a trophic factor that maintained neuronal survival. Our results highlight a neuron-glia interaction that is indispensable for network formation during a specific period in the developing brain.

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Authors: Ueno, M., Fujita, Y., Tanaka, T., Nakamura, Y., Kikuta, J., Ishii, M., & Yamashita, T.
Affiliations: Japan Science and Technology Agency (JP)
Asahikawa Medical University (JP)
Immunology Frontier Research Center, The University of Osaka (JP)
Corresponding author: Ueno, Masaki
Yamashita, Toshihide
ORCID: Ueno, Masaki
Fujita, Yuki
Tanaka, Tatsuhide
Nakamura, Yuka
Kikuta, Junichi
Ishii, Masaru
Yamashita, Toshihide
Venue: Nature Neuroscience
Publisher: Springer Science and Business Media LLC
Published: 2013-03-24
Volume: 16
Issue: 5
Pages: 543-551
ISSN: 1097-6256 (ISSN-L)
1546-1726
DOI: 10.1038/nn.3358
OpenAlex: W2015412022
Cited by: 781
References: 50
Citations by year: 2026: 41 · 2025: 54 · 2024: 67 · 2023: 61 · 2022: 65 · 2021: 81 · 2020: 82 · 2019: 69 · 2018: 64 · 2017: 52 · 2016: 50 · 2015: 43 · 2014: 41 · 2013: 11
FWCI: 26.72
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature Neuroscience (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords: microglia, neuronal survival, postnatal brain development, insulin-like growth factor I, trophic support, neuron-glia interactions, CX3CR1, apoptosis, minocycline, callosal projection neurons, microglial activation
MeSH terms: CX3C Chemokine Receptor 1, Animals, Animals, Newborn, Calcium-Binding Proteins, Cell Survival, Cells, Cultured, Diphtheria Toxin, Embryo, Mammalian, Enzyme Inhibitors, Humans, Insulin-Like Growth Factor I, Mice, Inbred C57BL, Mice, Transgenic, Microfilament Proteins, Nerve Tissue Proteins, Neurons, Visual Cortex, Apoptosis, Microglia, Gene Expression Regulation, Developmental, Coculture Techniques, Receptors, Chemokine, CD11b Antigen, Mice, Calcium-Calmodulin-Dependent Protein Kinase Type 2

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Layer V cortical neurons require microglial support for survival during postnatal development

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