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Cited by 385Axon Guidance and Neuronal SignalingProtein Kinase Regulation and GTPase SignalingNeurogenesis and neuroplasticity mechanisms

The DOI matches its Crossref record; 38 of the 39 references it cites were found in a registry or an index.

The sequential activity of the GTPases Rap1B and Cdc42 determines neuronal polarity.

Schwamborn, Jens C, Püschel, Andreas W·2004·Nature Neuroscience
DOI 10.1038/nn1295
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Abstract

The establishment of a polarized morphology is an essential step in the differentiation of neurons with a single axon and multiple dendrites. In cultured rat hippocampal neurons, one of several initially indistinguishable neurites is selected to become the axon. Both phosphatidylinositol 3,4,5-trisphosphate and the evolutionarily conserved Par complex (comprising Par3, Par6 and an atypical PKC (aPKC) such as PKClambda or PKCzeta) are involved in axon specification. However, the initial signals that establish cellular asymmetry and the pathways that subsequently translate it into structural changes remain to be elucidated. Here we show that localization of the GTPase Rap1B to the tip of a single neurite is a decisive step in determining which neurite becomes the axon. Using GTPase mutants and RNA interference, we found that Rap1B is necessary and sufficient to initiate the development of axons upstream of Cdc42 and the Par complex.

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Crossref · 10.1038/nn1295

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Metadata

Authors
Schwamborn, J. C., & Püschel, A. W.
Affiliations
University of Münster (DE)
Corresponding author
Püschel, Andreas W
ORCID
Venue
Nature Neuroscience
Publisher
Springer Science and Business Media LLC
Published
2004-08-01
Volume
7
Issue
9
Pages
923-929
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
DOI
10.1038/nn1295
OpenAlex
W2059346427
Cited by
385
References
39
Citations by year
2026: 1 · 2025: 3 · 2024: 5 · 2023: 5 · 2022: 15 · 2021: 8 · 2020: 8 · 2019: 10 · 2018: 15 · 2017: 18 · 2016: 18 · 2015: 16 · 2014: 13 · 2013: 20 · 2012: 28
FWCI
11.00
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • Open Repository and Bibliography (University of Luxembourg) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
Rap1b, Cdc42, neuronal polarity, axon specification, GTPase, Par complex, phosphatidylinositol 3,4,5-trisphosphate, atypical PKC, hippocampal neurons, neuronal differentiation
MeSH terms
Animals, Animals, Newborn, Antibodies, Monoclonal, Axons, Carrier Proteins, Cells, Cultured, Dendrites, Embryo, Mammalian, Hippocampus, Immunohistochemistry, Luminescent Proteins, Microtubule-Associated Proteins, Nerve Tissue Proteins, Neurons, Phalloidine, Protein Kinase C, Proto-Oncogene Proteins, RNA, Messenger, Time Factors, Transfection, Cytochalasin D, Protein Serine-Threonine Kinases, Gene Transfer Techniques, Body Patterning, Reverse Transcriptase Polymerase Chain Reaction, GTP Phosphohydrolases, rap GTP-Binding Proteins, rhoA GTP-Binding Protein, cdc42 GTP-Binding Protein, RNA Interference, Green Fluorescent Proteins, Proto-Oncogene Proteins c-akt, Mice, Rats

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