Quantification of F2-isoprostanes as a biomarker of oxidative stress
Abstract
Oxidant stress has been implicated in a wide variety of disease processes. One method to quantify oxidative injury is to measure lipid peroxidation. Quantification of a group of prostaglandin F(2alpha)-like compounds derived from the nonezymatic oxidation of arachidonic acid, termed the F2-isoprostanes (F2-IsoPs), provides an accurate assessment of oxidative stress both in vitro and in vivo. In fact, in a recent independent study sponsored by the National Institutes of Health (NIH), F2-IsoPs were shown to be the most reliable index of in vivo oxidant stress when compared against other well known biomarkers. This protocol details our laboratory's method to quantify F2-IsoPs in biological fluids and tissues using gas chromatography-mass spectrometry (GC-MS). This procedure can be completed for 12-15 samples in 6-8 h.
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Amitriptyline can exhibit prooxidant activity as a result to coenzyme Q10 life shortage and lipid peroxidation potentiation.
“Also, amitriptyline can exhibit prooxidant activity as a result to coenzyme Q10 life shortage and lipid peroxidation potentiation [267, 268].”
From Oxidative Stress Implications in the Affective Disorders: Main Biomarkers, Animal Models Relevance, Genetic Perspectives, and Antioxidant Approaches (2016) · cites this paper as [268]
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- Authors
- Milne, G. L., Sanchez, S. C., Musiek, E. S., & Morrow, J. D.
- Affiliations
- Vanderbilt University (US)
- Corresponding author
- Morrow, Jason D
- Venue
- Nature Protocols
- Published
- 2007-01
- Volume
- 2
- Issue
- 1
- Pages
- 221-226
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2008566131
- Cited by
- 333
- References
- 13
- Citations by year
- 2026: 10 · 2025: 15 · 2024: 10 · 2023: 10 · 2022: 17 · 2021: 18 · 2020: 14 · 2019: 20 · 2018: 13 · 2017: 18 · 2016: 21 · 2015: 23 · 2014: 19 · 2013: 31 · 2012: 23
- FWCI
- 12.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Protocols (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (CA77839, P01CA077839)
- National Institute of General Medical Sciences (GM154312)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK 48831, R01DK048831)
- National Institute of Environmental Health Sciences (ES13125, P01ES013125)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Keywords
- F2-isoprostanes, oxidative stress, lipid peroxidation, arachidonic acid oxidation, biomarkers, gas chromatography-mass spectrometry, biological fluids, tissue analysis
- MeSH terms
- Chemistry Techniques, Analytical, Humans, Gas Chromatography-Mass Spectrometry, Biomarkers, Oxidative Stress, F2-Isoprostanes
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