High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding

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Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies

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A highly specific SpCas9 variant is identified by in vivo screening in yeast

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Homology-Integrated CRISPR–Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

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Elevated expression of TcHMA3 plays a key role in the extreme Cd tolerance in a Cd‐hyperaccumulating ecotype of Thlaspi caerulescens

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The Yeast Ski Complex: Crystal Structure and RNA Channeling to the Exosome Complex

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Gene regulatory network reconstruction using single-cell RNA sequencing of barcoded genotypes in diverse environments

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Deep learning of the regulatory grammar of yeast 5′ untranslated regions from 500,000 random sequences

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Epigenetic silencing mediates mitochondria stress-induced longevity

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In vivo targeting of de novo DNA methylation by histone modifications in yeast and mouse

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Protein generation with evolutionary diffusion: sequence is all you need

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Measuring the sequence-affinity landscape of antibodies with massively parallel titration curves

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Spatiotemporal coordination of transcription preinitiation complex assembly in live cells

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Ro KSN, a floral repressor, forms protein complexes with Ro FD and Ro FT to regulate vegetative and reproductive development in rose

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A Genome-Wide Map of Mitochondrial DNA Recombination in Yeast

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PICALM Rescues Endocytic Defects Caused by the Alzheimer’s Disease Risk Factor APOE4

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The yeast mitochondrial pyruvate carrier is a hetero-dimer in its functional state

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Deep learning–guided design of dynamic proteins

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The two-component response regulator Ssk1 and the mitogen-activated protein kinase Hog1 control antifungal drug resistance and cell wall architecture of Candida auris

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Starships are active eukaryotic transposable elements mobilized by a new family of tyrosine recombinases

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A comprehensive map of human glucokinase variant activity

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The Iron Deficiency-Regulated Small Protein Effector FEP3/IRON MAN1 Modulates Interaction of BRUTUS-LIKE1 With bHLH Subgroup IVc and POPEYE Transcription Factors

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Slow release of a synthetic auxin induces formation of adventitious roots in recalcitrant woody plants

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Key features of inhibitor binding to the human mitochondrial pyruvate carrier hetero-dimer

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Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle

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Production of 14α-hydroxysteroids by a recombinant Saccharomyces cerevisiae biocatalyst expressing of a fungal steroid 14α-hydroxylation system

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Asymmetrical dose responses shape the evolutionary trade-off between antifungal resistance and nutrient use

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The dynamics of adaptation to stress from standing genetic variation and de novo mutations

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Design of diverse, functional mitochondrial targeting sequences across eukaryotic organisms using variational autoencoder

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Evolution of reduced co-activator dependence led to target expansion of a starvation response pathway

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Production of (−)-α-Bisabolol in Metabolically Engineered Saccharomyces Cerevisiae

Kim, T. Y., Park, H., Kim, S.-K., Kim, S.-J., & Park, Y.-C. (2021) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2021.08.008 · Cited by 23

Systematic analysis of NDUFAF6 in complex I assembly and mitochondrial disease

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Characterizing glucokinase variant mechanisms using a multiplexed abundance assay

Gersing, S., Schulze, T. K., Cagiada, M., Stein, A., Roth, F. P., Lindorff-Larsen, K., & Hartmann-Petersen, R. (2024) · Genome Biology · doi:10.1186/s13059-024-03238-2 · Cited by 23

Disordered clock protein interactions and charge blocks turn an hourglass into a persistent circadian oscillator

Jankowski, M. S., Griffith, D., Shastry, D. G., Pelham, J. F., Ginell, G. M., Thomas, J., Karande, P., Holehouse, A. S., & Hurley, J. M. (2024) · Nature Communications · doi:10.1038/s41467-024-47761-z · Cited by 23

Membrane potential stimulates ADP import and ATP export by the mitochondrial ADP/ATP carrier due to its positively charged binding site

Mavridou, V., King, M. S., Bazzone, A., Springett, R., & Kunji, E. R. S. (2024) · Science Advances · doi:10.1126/sciadv.adp7725 · Cited by 20

DIP2 is a unique regulator of diacylglycerol lipid homeostasis in eukaryotes

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Engineering rice Nramp5 modifies cadmium and manganese uptake selectivity using yeast assay system

Inoue, J., Teramoto, T., Kazama, T., & Nakamura, T. (2024) · Frontiers in Plant Science · doi:10.3389/fpls.2024.1482099 · Cited by 18

ProtWave-VAE: Integrating Autoregressive Sampling with Latent-Based Inference for Data-Driven Protein Design

Praljak, N., Lian, X., Ranganathan, R., & Ferguson, A. L. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.3c00261 · Cited by 18

Deep mutational scanning quantifies DNA binding and predicts clinical outcomes of PAX6 variants

McDonnell, A. F., Plech, M., Livesey, B. J., Gerasimavicius, L., Owen, L. J., Hall, H. N., FitzPatrick, D. R., Marsh, J. A., & Kudla, G. (2024) · Molecular Systems Biology · doi:10.1038/s44320-024-00043-8 · Cited by 16

Accurate single-domain scaffolding of three nonoverlapping protein epitopes using deep learning

Castro, K. M., Watson, J. L., Wang, J., Southern, J., Ayardulabi, R., Georgeon, S., Rosset, S., Baker, D., & Correia, B. E. (2026) · Nature Chemical Biology · doi:10.1038/s41589-025-02083-z · Cited by 13

The PQ-loop protein Any1 segregates Drs2 and Neo1 functions required for viability and plasma membrane phospholipid asymmetry

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Fitness landscape of substrate-adaptive mutations in evolved amino acid-polyamine-organocation transporters

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Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei

Nogueira, K. M. V., Mendes, V., Kamath, K. S., Cheruku, A., Oshiquiri, L. H., de Paula, R. G., Carraro, C., Pedersoli, W. R., Pereira, L. M. S., Vieira, L. C., et al. (2024) · Microbial Cell Factories · doi:10.1186/s12934-023-02279-9 · Cited by 12

Substrate-specific effects of natural genetic variation on proteasome activity

Collins, M. A., Avery, R., & Albert, F. W. (2023) · PLOS Genetics · doi:10.1371/journal.pgen.1010734 · Cited by 11

Microbes vary strategically in their metalation of mononuclear enzymes

Rohaun, S. K., Sethu, R., & Imlay, J. A. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2401738121 · Cited by 11

Variation in ubiquitin system genes creates substrate-specific effects on proteasomal protein degradation

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Deep learning guided design of dynamic proteins

Guo, A. B., Akpinaroglu, D., Kelly, M. J. S., & Kortemme, T. (2024) · doi:10.1101/2024.07.17.603962 · Cited by 9

The riboflavin biosynthetic pathway as a novel target for antifungal drugs against Candida species

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The conserved Arabidopsis PM19L1 protein functions as an osmosensor and regulator of dormancy and germination

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Specialization Restricts the Evolutionary Paths Available to Yeast Sugar Transporters

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Mutational scan inferred binding energetics and structure in intrinsically disordered protein CcdA

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A random mutagenesis screen enriched for missense mutations in bacterial effector proteins

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Systematic characterization of indel variants using a yeast-based protein folding sensor

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Fitness landscape of substrate-adaptive mutations in evolved amino acid-polyamine-organocation transporters

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Fitness landscape of substrate-adaptive mutations in evolved APC transporters

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Genetic requirements for cell division in a genomically minimal cell

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