Cited by 215RNA Interference and Gene DeliveryRNA Research and SplicingRNA regulation and disease

Small Interfering RNA-Mediated Gene Silencing in T Lymphocytes

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McManus, Michael T, Haines, Brian B, Dillon, Christopher P, Whitehurst, Charles E, van Parijs, Luk, Chen, Jianzhu, Sharp, Phillip A·2002·The Journal of Immunology

Abstract

Introduction of small interfering RNAs (siRNAs) into a cell can cause a specific interference of gene expression known as RNA interference (RNAi). However, RNAi activity in lymphocytes and in normal primary mammalian cells has not been thoroughly demonstrated. In this report, we show that siRNAs complementary to CD4 and CD8α specifically reduce surface expression of these coreceptors and their respective mRNA in a thymoma cell line model. We show that RNAi activity is only caused by a subset of siRNAs complementary to the mRNA target and that ineffective siRNAs can compete with effective siRNAs. Using primary differentiated T lymphocytes, we provide the first evidence of siRNA-mediated RNAi gene silencing in normal nontransformed somatic mammalian lymphocytes.

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Crossref · 10.4049/jimmunol.169.10.5754

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  • Naïve T cells and Th17 cells cannot be efficiently transfected in vitro by traditional methods without altering their phenotype or function.

    “naïve T cells and Th17 cells cannot be transfected effectively in vitro by traditional methods without changing their phenotype or function”

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Authors
McManus, M. T., Haines, B. B., Dillon, C. P., Whitehurst, C. E., van Parijs, L., Chen, J., & Sharp, P. A.
Affiliations
  • Massachusetts Institute of Technology (US)
  • Center for Cancer Research, Massachusetts Institute of Technology , Cambridge, MA 02139
Venue
The Journal of Immunology
Published
2002-11
Volume
169
Issue
10
Pages
5754-5760
ISSN
  • 0022-1767 (ISSN-L)
  • 1550-6606
OpenAlex
W2157078151
Cited by
215
References
40
Citations by year
2025: 1 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 5 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 6 · 2013: 2 · 2012: 14
FWCI
9.97
Type
journal article
Language
en
Open access
Locations
  • The Journal of Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Cancer Research Institute
  • Arthritis Foundation
  • National Institutes of Health (CA42063)
  • National Cancer Institute (P01CA042063, P01 CA 42063)
  • National Institute of General Medical Sciences (R37-GM34277, R37GM034277)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords
siRNA, RNA interference, gene silencing, T cells, CD4, CD8A, primary lymphocytes
MeSH terms
Animals, Luminescent Proteins, Membrane Glycoproteins, Mice, Inbred C57BL, Mice, Mutant Strains, Mice, Transgenic, RNA, Messenger, Spleen, T-Lymphocytes, Thymus Gland, Tumor Cells, Cultured, CD4 Antigens, CD8 Antigens, Gene Silencing, RNA Stability, RNA, Small Interfering, Green Fluorescent Proteins, Mice

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