Small Interfering RNA-Mediated Gene Silencing in T Lymphocytes
Open accessAbstract
Introduction of small interfering RNAs (siRNAs) into a cell can cause a specific interference of gene expression known as RNA interference (RNAi). However, RNAi activity in lymphocytes and in normal primary mammalian cells has not been thoroughly demonstrated. In this report, we show that siRNAs complementary to CD4 and CD8α specifically reduce surface expression of these coreceptors and their respective mRNA in a thymoma cell line model. We show that RNAi activity is only caused by a subset of siRNAs complementary to the mRNA target and that ineffective siRNAs can compete with effective siRNAs. Using primary differentiated T lymphocytes, we provide the first evidence of siRNA-mediated RNAi gene silencing in normal nontransformed somatic mammalian lymphocytes.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Naïve T cells and Th17 cells cannot be efficiently transfected in vitro by traditional methods without altering their phenotype or function.
“naïve T cells and Th17 cells cannot be transfected effectively in vitro by traditional methods without changing their phenotype or function”
From Dynamic regulatory network controlling TH17 cell differentiation (2013) · cites this paper as [22]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429 · see citing works
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
Lee, R. C., Feinbaum, R. L., & Ambros, V. (1993) · Cell · doi:10.1016/0092-8674(93)90529-y · Cited by 12,948 · see citing works
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456 · see citing works
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
Reinhart, B. J., Slack, F. J., Basson, M., Pasquinelli, A. E., Bettinger, J. C., Rougvie, A. E., Horvitz, H. R., & Ruvkun, G. (2000) · Nature · doi:10.1038/35002607 · Cited by 4,989 · see citing works
Hannon, G. J. (2002) · Nature · doi:10.1038/418244a · Cited by 3,583 · see citing works
Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA
Pasquinelli, A. E., Reinhart, B. J., Slack, F. J., Martindale, M. Q., Kuroda, M. I., Maller, B., Hayward, D. C., Ball, E. E., Degnan, B. M., Müller, P., et al. (2000) · Nature · doi:10.1038/35040556 · Cited by 2,568 · see citing works
Hutvágner, G., McLachlan, J., Pasquinelli, A. E., Bálint, E., Tuschl, T., & Zamore, P. D. (2001) · Science · doi:10.1126/science.1062961 · Cited by 2,287 · see citing works
Ambros, V. (2001) · Cell · doi:10.1016/s0092-8674(01)00616-x · Cited by 1,704 · see citing works
Grishok, A., Pasquinelli, A. E., Conte, D., Li, N., Parrish, S., Ha, I., Baillie, D. L., Fire, A., Ruvkun, G., & Mello, C. C. (2001) · Cell · doi:10.1016/s0092-8674(01)00431-7 · Cited by 1,600 · see citing works
Gene silencing in mammals by small interfering RNAs
McManus, M. T., & Sharp, P. A. (2002) · Nature Reviews Genetics · doi:10.1038/nrg908 · Cited by 1,400 · see citing works
Self-citationOn the Role of RNA Amplification in dsRNA-Triggered Gene Silencing
Sijen, T., Fleenor, J., Simmer, F., Thijssen, K. L., Parrish, S., Timmons, L., Plasterk, R. H. A., & Fire, A. Z. (2001) · Cell · doi:10.1016/s0092-8674(01)00576-1 · Cited by 1,280 · see citing works
Analysis of gene function in somatic mammalian cells using small interfering RNAs
Elbashir, S. M. (2002) · Methods · doi:10.1016/s1046-2023(02)00023-3 · Cited by 1,094 · see citing works
Caplen, N. J., Parrish, S., Imani, F., Fire, A. Z., & Morgan, R. A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.171251798 · Cited by 1,042 · see citing works
ATR and ATRIP: Partners in Checkpoint Signaling
Cortez, D., Guntuku, S., Qin, J., & Elledge, S. J. (2001) · Science · doi:10.1126/science.1065521 · Cited by 985 · see citing works
Olsen, P. H., & Ambros, V. (1999) · Developmental Biology · doi:10.1006/dbio.1999.9523 · Cited by 969 · see citing works
Targeted mRNA degradation by double-stranded RNA in vitroPreprint
Tuschl, T., Zamore, P. D., Lehmann, R., Bartel, D. P., & Sharp, P. A. (1999) · Genes & Development · doi:10.1101/gad.13.24.3191 · Cited by 843 · see citing works
Self-citationsiRNA-directed inhibition of HIV-1 infection
Novina, C. D., Murray, M. F., Dykxhoorn, D. M., Beresford, P. J., Riess, J. W., Lee, S., Collman, R. G., Lieberman, J., Shankar, P., & Sharp, P. A. (2002) · Nature Medicine · doi:10.1038/nm725 · Cited by 775 · see citing works
Self-citationPhosphorylation-Dependent Ubiquitination of Cyclin E by the SCF Fbw7 Ubiquitin Ligase
Koepp, D. M., Schaefer, L. K., Ye, X., Keyomarsi, K., Chu, C., Harper, J. W., & Elledge, S. J. (2001) · Science · doi:10.1126/science.1065203 · Cited by 764 · see citing works
Specific interference with gene function by double-stranded RNA in early mouse development
Wianny, F., & Zernicka‐Goetz, M. (1999) · Nature Cell Biology · doi:10.1038/35000016 · Cited by 722 · see citing works
Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor
Holen, T. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.8.1757 · Cited by 706 · see citing works
RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans
Montgomery, M. K., Xu, S., & Fire, A. Z. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.26.15502 · Cited by 580 · see citing works
Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference
Svoboda, P., Stein, P., Hayashi, H., & Schultz, R. M. (2000) · Development · doi:10.1242/dev.127.19.4147 · Cited by 407 · see citing works
Functional Anatomy of a dsRNA Trigger
Parrish, S., Fleenor, J., Xu, S., Mello, C. C., & Fire, A. Z. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)00106-4 · Cited by 401 · see citing works
RNAi as Random Degradative PCR
Lipardi, C., Qin, W., & Paterson, B. M. (2001) · Cell · doi:10.1016/s0092-8674(01)00537-2 · Cited by 369 · see citing works
RNA interference: listening to the sound of silence.
Zamore, P. D. (2001) · Nature Structural Biology · doi:10.1038/nsb0901-746 · Cited by 368 · see citing works
Extracellular Export of Sphingosine Kinase-1 Enzyme
Ancellin, N., Colmont, C. S., Su, J. H., Li, Q., Mittereder, N., Chae, S.-S., Stefansson, S., Liau, G., & Hla, T. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m102841200 · Cited by 280 · see citing works
Control of Developmental Timing by MicroRNAs and Their Targets
Pasquinelli, A. E., & Ruvkun, G. (2002) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.18.012502.105832 · Cited by 271 · see citing works
Bernstein, E., Denli, A. M., & Hannon, G. J. (2001) · PubMed Central · Cited by 225 · see citing works
Biosynthesis of the Linkage Region of Glycosaminoglycans
Bai, X., Zhou, D., Brown, J. R., Crawford, B. E., Hennet, T., & Esko, J. D. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m107339200 · Cited by 190 · see citing works
Li, L., Mao, J., Sun, L., Liu, W., & Wu, D. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m111131200 · Cited by 168 · see citing works
Banerjee, D., & Slack, F. J. (2002) · BioEssays · doi:10.1002/bies.10046 · Cited by 166 · see citing works
Großhans, H., & Slack, F. J. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200111033 · Cited by 141 · see citing works
Ohta, T., Essner, R., Ryu, J.-H., Palazzo, R. E., Uetake, Y., & Kuriyama, R. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200108088 · Cited by 136 · see citing works
RNA interference: antiviral defense and genetic tool
Cullen, B. R. (2002) · Nature Immunology · doi:10.1038/ni0702-597 · Cited by 126 · see citing works
MicroRNAs: Hidden in the Genome
Moss, E. G. (2002) · Current Biology · doi:10.1016/s0960-9822(02)00708-x · Cited by 123 · see citing works
Norment, A. M., Lönberg, N., Lacy, E., & Littman, D. R. (1989) · The Journal of Immunology · doi:10.4049/jimmunol.142.9.3312 · Cited by 52 · see citing works
A secreted form of the human lymphocyte cell surface molecule CD8 arises from alternative splicing.
Giblin, P. A., Ledbetter, J. A., & Kavathas, P. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.3.998 · Cited by 52 · see citing works
Mombaerts, P., Terhorst, C., Jacks, T., Tonegawa, S., & Sancho, J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7420 · Cited by 50 · see citing works
Wood, H. B. (2002) · Nature reviews. Neuroscience · doi:10.1038/nrn707 · Cited by 17 · see citing works
Flagged first, then by citations
1 of the 40 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Rubinson, D. A., Dillon, C. P., Kwiatkowski, A. V., Sievers, C., Yang, L., Kopinja, J. E., Rooney, D. L., Zhang, M., Ihrig, M. M., McManus, M. T., et al. (2003) · Nature Genetics · doi:10.1038/ng1117 · Cited by 1,499 · see citing works
Self-citationKilling the messenger: short RNAs that silence gene expression
Dykxhoorn, D. M., Novina, C., & Sharp, P. (2003) · Nature reviews. Molecular cell biology · doi:10.1038/nrm1129 · Cited by 1,290 · see citing works
Self-citationIntracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts
Stewart, M. P., Langer, R., & Jensen, K. F. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00678 · Cited by 748 · see citing works
Dynamic regulatory network controlling TH17 cell differentiation
Yosef, N., Shalek, A. K., Gaublomme, J. T., Jin, H., Lee, Y., Awasthi, A., Wu, C., Karwacz, K., Xiao, S., Jorgolli, M., et al. (2013) · Nature · doi:10.1038/nature11981 · Cited by 682 · see citing works
siRNA Delivery into Human T Cells and Primary Cells with Carbon‐Nanotube Transporters
Liu, Z., Winters, M. A., Holodniy, M., & Dai, H. (2007) · Angewandte Chemie International Edition · doi:10.1002/anie.200604295 · Cited by 666 · see citing works
Specificity of short interfering RNA determined through gene expression signatures
Semizarov, D., Frost, L., Sarthy, A. V., Kroeger, P., Halbert, D. N., & Fesik, S. W. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1131959100 · Cited by 500 · see citing works
Bidère, N., Lorenzo, H. K., Carmona, S., Laforge, M., Harper, F., Dumont, C., & Senik, A. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m301911200 · Cited by 435 · see citing works
An algorithm for selection of functional siRNA sequences
Amarzguioui, M., & Prydz, H. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.02.157 · Cited by 434 · see citing works
High-throughput RNAi screening in cultured cells: a user's guide
Echeverri, C. J., & Perrimon, N. (2006) · Nature Reviews Genetics · doi:10.1038/nrg1836 · Cited by 380 · see citing works
On the art of identifying effective and specific siRNAs
Pei, Y., & Tuschl, T. (2006) · Nature Methods · doi:10.1038/nmeth911 · Cited by 302 · see citing works
Photothermal nanofibres enable safe engineering of therapeutic cells
Xiong, R., Hua, D., Hoeck, J. V., Berdecka, D., Léger, L., Munter, S. D., Fraire, J. C., Raes, L., Harizaj, A., Sauvage, F., et al. (2021) · Nature Nanotechnology · doi:10.1038/s41565-021-00976-3 · Cited by 288 · see citing works
Improving the efficiency of RNA interference in mammals
Mittal, V. (2004) · Nature Reviews Genetics · doi:10.1038/nrg1323 · Cited by 284 · see citing works
Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins
McNaughton, B. R., Cronican, J. J., Thompson, D. B., & Liu, D. R. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0807883106 · Cited by 267 · see citing works
McKinlay, C. J., Benner, N. L., Haabeth, O. A. W., Waymouth, R. M., & Wender, P. A. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1805358115 · Cited by 240 · see citing works
Miller, S., Miyata, K., Brown, S. J., & Tomoyasu, Y. (2012) · PLoS ONE · doi:10.1371/journal.pone.0047431 · Cited by 217 · see citing works
Therapeutic Potential of RNA Interference
Stevenson, M. (2004) · New England Journal of Medicine · doi:10.1056/nejmra045004 · Cited by 212 · see citing works
Grimm, D., Wang, L., Lee, J. S., Schürmann, N., Gu, S., Börner, K., Storm, T. A., & Kay, M. A. (2010) · Journal of Clinical Investigation · doi:10.1172/jci43565 · Cited by 196 · see citing works
Davies, J. A. (2003) · Human Molecular Genetics · doi:10.1093/hmg/ddh015 · Cited by 190 · see citing works
Shalek, A. K., Gaublomme, J. T., Wang, L., Yosef, N., Chevrier, N., Andersen, M. S., Robinson, J. T., Pochet, N. L. M. M., Neuberg, D. S., Gertner, R. S., et al. (2012) · Nano Letters · doi:10.1021/nl3042917 · Cited by 179 · see citing works
Banerjea, A. C., Li, M.-J., Bauer, G., Remling‐Mulder, L., Lee, N.-S., Rossi, J. J., & Akkina, R. (2003) · Molecular Therapy · doi:10.1016/s1525-0016(03)00140-0 · Cited by 168 · see citing works
Scholten, K. B. J., Kramer, D., Taanman-Kueter, E. W., Graf, M., Schoedl, T., Meijer, C. J. L. M., Schreurs, M. W. J., & Hooijberg, E. (2006) · Clinical Immunology · doi:10.1016/j.clim.2005.12.009 · Cited by 164 · see citing works
Establishment of conditional vectors for hairpin siRNA knockdowns
Matsukura, S. (2003) · Nucleic Acids Research · doi:10.1093/nar/gng077 · Cited by 164 · see citing works
The therapeutic potential of RNA interference
Uprichard, S. L. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.08.004 · Cited by 161 · see citing works
Mammalian RNAi: a Practical Guide
Sandy, P., Ventura, A., & Jacks, T. (2005) · BioTechniques · doi:10.2144/05392rv01 · Cited by 149 · see citing works
RNA interference in biology and disease
Sledz, C. A., & Williams, B. R. G. (2005) · Blood · doi:10.1182/blood-2004-12-4643 · Cited by 144 · see citing works
A future for transgenic livestock
Clark, A., & Whitelaw, B. (2003) · Nature Reviews Genetics · doi:10.1038/nrg1183 · Cited by 134 · see citing works
Immune Modulation by Silencing IL-12 Production in Dendritic Cells Using Small Interfering RNA
Hill, J. A., Ichim, T. E., Kusznieruk, K. P., Li, M., Huang, X., Yan, X., Zhong, R., Cairns, E., Bell, D., & Min, W.-P. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.171.2.691 · Cited by 132 · see citing works
Ablation of oncogenic ALK is a viable therapeutic approach for anaplastic large-cell lymphomas
Piva, R., Chiarle, R., Manazza, A. D., Taulli, R., Simmons, W. R., Ambrogio, C., d’Escamard, V., Pellegrino, E., Ponzetto, C., Palestro, G., et al. (2005) · Blood · doi:10.1182/blood-2005-05-2125 · Cited by 128 · see citing works
Prechtel, A. T., Turza, N. M., Theodoridis, A. A., & Steinkasserer, A. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.178.9.5454 · Cited by 125 · see citing works
Round, J. L., Tomassian, T., Zhang, M., Patel, V. P., Schoenberger, S. P., & Miceli, M. C. (2005) · The Journal of Experimental Medicine · doi:10.1084/jem.20041428 · Cited by 123 · see citing works
Holen, T. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg338 · Cited by 121 · see citing works
Chiu, Y.-L., Cao, H., Jacqué, J.-M., Stevenson, M., & Rana, T. M. (2004) · Journal of Virology · doi:10.1128/jvi.78.5.2517-2529.2004 · Cited by 120 · see citing works
Competition for RISC binding predicts in vitro potency of siRNA
Koller, E., Propp, S. S., Murray, H., Lima, W. F., Bhat, B., Prakash, T. P., Allerson, C. R., Swayze, E. E., Marcusson, E. G., & Dean, N. M. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl589 · Cited by 114 · see citing works
Laser-assisted photoporation: fundamentals, technological advances and applications
Xiong, R., Samal, S. K., Demeester, J., Skirtach, A. G., Smedt, S. C. D., & Braeckmans, K. (2016) · Advances in Physics X · doi:10.1080/23746149.2016.1228476 · Cited by 108 · see citing works
Pathak, S. K., Basu, S., Bhattacharyya, A., Pathak, S., Kundu, M., & Basu, J. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m506471200 · Cited by 107 · see citing works
RNA Interference: A Potent Tool for Gene-Specific Therapeutics
Ichim, T. E., Li, M., Qian, H., Popov, I. A., Rycerz, K., Zheng, X., White, D. J., Zhong, R., & Min, W. (2004) · American Journal of Transplantation · doi:10.1111/j.1600-6143.2004.00530.x · Cited by 107 · see citing works
Interfering with disease: opportunities and roadblocks to harnessing RNA interference
Lieberman, J., Song, E., Lee, S., & Shankar, P. (2003) · Trends in Molecular Medicine · doi:10.1016/s1471-4914(03)00143-6 · Cited by 107 · see citing works
Ca 2+ /Calmodulin-Dependent Protein Kinase II Is a Modulator of CARMA1-Mediated NF-κB Activation
Ishiguro, K., Green, T. J., Rapley, J., Wachtel, H., Giallourakis, C. C., Landry, A. L., Cao, Z., Lu, N., Ando, T., Goto, H., et al. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.02469-05 · Cited by 104 · see citing works
Xu, W., Jiang, X., & Huang, L. (2019) · Elsevier eBooks · doi:10.1016/b978-0-444-64046-8.00282-2 · Cited by 101 · see citing works
Richard, S., MOREL, M., & Cléroux, P. (2005) · Biochemical Journal · doi:10.1042/bj20040373 · Cited by 101 · see citing works
Innovative nanotechnologies for the delivery of oligonucleotides and siRNA
Toub, N., Malvy, C., Fattal, E., & Couvreur, P. (2006) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2006.07.093 · Cited by 96 · see citing works
Dillon, C. P., Sandy, P., Nencioni, A., Kissler, S., Rubinson, D. A., & Parijs, L. V. (2004) · Annual Review of Physiology · doi:10.1146/annurev.physiol.67.040403.130716 · Cited by 95 · see citing works
Self-citationReduced levels of Ago2 expression result in increased siRNA competition in mammalian cells
Vickers, T. A., Lima, W. F., Nichols, J. G., & Crooke, S. T. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm663 · Cited by 93 · see citing works
TDP43 depletion rescues aberrant CFTR exon 9 skipping
Ayala, Y. M., Pagani, F., & Baralle, F. E. (2006) · FEBS Letters · doi:10.1016/j.febslet.2006.01.052 · Cited by 88 · see citing works
Discovery, biology and therapeutic potential of RNA interference, microRNA and antagomirs
Yang, M., & Mattes, J. E. (2007) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2007.08.004 · Cited by 87 · see citing works
Rodrı́guez, P. C., Hernandez, C. P., Morrow, K. D., Sierra, R. A., Zabaleta, J., Wyczechowska, D. D., & Ochoa, A. C. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.1001224 · Cited by 85 · see citing works
Izquierdo, J. M., & Valcárcel, J. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.c600198200 · Cited by 84 · see citing works
Quantitative evaluation of siRNA delivery in vivo
Pei, Y., Hancock, P. J., Zhang, H., Bartz, R., Cherrin, C., Innocent, N., Pomerantz, C. J., Seitzer, J. L., Koser, M. L., Abrams, M. T., et al. (2010) · RNA · doi:10.1261/rna.2255810 · Cited by 82 · see citing works
siRNA stabilization prolongs gene knockdown in primary T lymphocytes
Mantei, A., Rutz, S., Janke, M., Kirchhoff, D., Jung, U., Patzel, V., Vogel, U., Rudel, T., Andreou, I., Weber, M., et al. (2008) · European Journal of Immunology · doi:10.1002/eji.200738075 · Cited by 80 · see citing works
An Efficient System for Cap- and Poly(A)-Dependent Translation In Vitro
Svitkin, Y. V., & Sonenberg, N. (2004) · Humana Press eBooks · doi:10.1385/1-59259-750-5:155 · Cited by 80 · see citing works
Optimization of electroporation and other non‐viral gene delivery strategies for T cells
Harris, E., & Elmer, J. J. (2020) · Biotechnology Progress · doi:10.1002/btpr.3066 · Cited by 77 · see citing works
RNAi as a gene therapy approach
Caplen, N. J. (2003) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.3.4.575 · Cited by 77 · see citing works
Karlas, A., Kurth, R., & Denner, J. (2004) · Virology · doi:10.1016/j.virol.2004.04.022 · Cited by 76 · see citing works
Oberdoerffer, P., Kanellopoulou, C., Heissmeyer, V., Paeper, C., Borowski, C., Aifantis, I., Rao, A., & Rajewsky, K. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.10.3896-3905.2005 · Cited by 76 · see citing works
Comparing photoporation and nucleofection for delivery of small interfering RNA to cytotoxic T cells
Wayteck, L., Xiong, R., Braeckmans, K., Smedt, S. C. D., & Raemdonck, K. (2017) · Journal of Controlled Release · doi:10.1016/j.jconrel.2017.08.002 · Cited by 75 · see citing works
The Design and Exogenous Delivery of siRNA for Post-transcriptional Gene Silencing
Gilmore, I. R., Fox, S. P., Hollins, A. J., Sohail, M. F., & Akhtar, S. (2004) · Journal of drug targeting · doi:10.1080/10611860400006257 · Cited by 73 · see citing works
A simple and cost-effective method for producing small interfering RNAs with high efficacy
Sohail, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gng038 · Cited by 73 · see citing works
Use of siRNAs to prevent and treat influenza virus infection
Ge, Q., Eisen, H. N., & Chen, J. (2004) · Virus Research · doi:10.1016/j.virusres.2004.01.013 · Cited by 72 · see citing works
Self-citationAntiviral Activity of CYC202 in HIV-1-infected Cells
Agbottah, E. T., de la Fuente, C., Nekhai, S., Barnett, A. L., Gianella-Borradori, A., Pumfery, A. M., & Kashanchi, F. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m406435200 · Cited by 70 · see citing works
Laderach, D. J., Compagno, D., Danos, O., Vainchenker, W., & Galy, A. H. M. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.171.4.1750 · Cited by 70 · see citing works
siRNA Delivery into Human T Cells and Primary Cells with Carbon‐Nanotube Transporters
Liu, Z., Winters, M. A., Holodniy, M., & Dai, H. (2007) · Angewandte Chemie · doi:10.1002/ange.200604295 · Cited by 67 · see citing works
Dissecting HIV-1 through RNA interference
Stevenson, M. (2003) · Nature reviews. Immunology · doi:10.1038/nri1227 · Cited by 66 · see citing works
RNA Interference by Short Hairpin RNAs Expressed in Vertebrate Cells
Hannon, G. J., & Conklin, D. S. (2004) · Humana Press eBooks · doi:10.1385/1-59259-750-5:255 · Cited by 65 · see citing works
Okuhara, A., Nakasa, T., Shibuya, H., Niimoto, T., Adachi, N., Deie, M., & Ochi, M. (2011) · Modern Rheumatology · doi:10.1007/s10165-011-0536-2 · Cited by 64 · see citing works
Liu, G., Ng, H., Akasaki, Y., Yuan, X., Ehtesham, M., Yin, D., Black, K. L., & Yu, J. S. (2004) · European Journal of Immunology · doi:10.1002/eji.200425081 · Cited by 64 · see citing works
Okuhara, A., Nakasa, T., Shibuya, H., Niimoto, T., Adachi, N., Deie, M., & Ochi, M. (2012) · Modern Rheumatology · doi:10.3109/s10165-011-0536-2 · Cited by 61 · see citing works
Efficient nonviral gene delivery into primary lymphocytes from rats and mice
Goffinet, C., & Keppler, O. T. (2006) · The FASEB Journal · doi:10.1096/fj.05-4651fje · Cited by 60 · see citing works
RNAi screening: tips and techniques
Sharma, S., & Rao, A. (2009) · Nature Immunology · doi:10.1038/ni0809-799 · Cited by 67 · see citing works
Integrated siRNA design based on surveying of features associated with high RNAi effectiveness
Gong, W., Ren, Y., Xu, Q., Wang, Y., Lin, D., Zhou, H., & Li, T. (2006) · BMC Bioinformatics · doi:10.1186/1471-2105-7-516 · Cited by 56 · see citing works
Using drug-excipient interactions for siRNA delivery
Bruno, K. (2011) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2011.09.003 · Cited by 56 · see citing works
Inhibition of hepatitis B virus replication by stably expressed shRNA
Chen, Y., Du, D., Wu, J., Chan, C.-P., Tan, Y., Kung, H., & He, M. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2003.10.009 · Cited by 56 · see citing works
Pelosi, M., Marampon, F., ZANI, B. M., Prudente, S., Perlas, E., Caputo, V., Cianetti, L., Berno, V., Narumiya, S., Kang, S. W., et al. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.01735-06 · Cited by 56 · see citing works
Lestrell, E., Patolsky, F., Voelcker, N. H., & Elnathan, R. (2019) · Materials Today · doi:10.1016/j.mattod.2019.08.012 · Cited by 55 · see citing works
Progress with Nonhuman Primate Embryonic Stem Cells1
Wolf, D. P., Kuo, H., Pau, K. ‐. F., & Lester, L. B. (2004) · Biology of Reproduction · doi:10.1095/biolreprod.104.029413 · Cited by 55 · see citing works
siDRM: an effective and generally applicable online siRNA design tool
Gong, W., Ren, Y., Zhou, H., Wang, Y., Kang, S., & Li, T. (2008) · Bioinformatics · doi:10.1093/bioinformatics/btn442 · Cited by 54 · see citing works
Freeley, M., & Long, A. (2013) · Biochemical Journal · doi:10.1042/bj20130950 · Cited by 54 · see citing works
Src, Fyn and Yes play differential roles in VEGF-mediated endothelial cell events
Werdich, X. Q., & Penn, J. S. (2006) · Angiogenesis · doi:10.1007/s10456-005-9021-x · Cited by 54 · see citing works
Lingor, P., Michel, U., Schöll, U., Bähr, M., & Kügler, S. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.01.170 · Cited by 53 · see citing works
Ehlers, M., Laule-Kilian, K., Petter, M., Aldrian, C. J., Grueter, B., Würch, A., Yoshida, N., Watanabe, T., Satake, M., & Steimle, V. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.171.7.3594 · Cited by 50 · see citing works
Laforge, M., Petit, F., Estaquier, J., & Senik, A. (2007) · Journal of Virology · doi:10.1128/jvi.00597-07 · Cited by 49 · see citing works
Zhao, M., Sun, D., Guan, Y., Wang, Z., Sang, D., Liu, M., Pu, Y., Fang, X., Wang, D., Huang, A., et al. (2016) · Journal of Neuroscience · doi:10.1523/jneurosci.4587-15.2016 · Cited by 48 · see citing works
Competition between Small RNAs: A Quantitative View
Loinger, A., Shemla, Y., Simon, I., Margalit, H., & Biham, O. (2012) · Biophysical Journal · doi:10.1016/j.bpj.2012.01.058 · Cited by 48 · see citing works
Chhabra, A., Chakraborty, N. G., & Mukherji, B. (2008) · Clinical Immunology · doi:10.1016/j.clim.2007.11.011 · Cited by 48 · see citing works
Wen, F., Liu, X., Kobayashi, T., Abe, S., Fang, Q., Kohyama, T., Ertl, R. F., Terasaki, Y., Manouilova, L. S., & Rennard, S. I. (2004) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2002-0249oc · Cited by 48 · see citing works
Ping, Y., Chu, C., Cao, H., Jacqué, J.-M., Stevenson, M., & Rana, T. M. (2004) · Retrovirology · doi:10.1186/1742-4690-1-46 · Cited by 48 · see citing works
Optimizing RNA interference for application in mammalian cells
Medema, R. H. (2004) · Biochemical Journal · doi:10.1042/bj20040260 · Cited by 48 · see citing works
Vang, T., Abrahamsen, H., Myklebust, S., Enserink, J. M., Prydz, H., Mustelin, T., Amarzguioui, M., & Taskén, K. (2004) · European Journal of Immunology · doi:10.1002/eji.200425036 · Cited by 47 · see citing works
Design, mechanism, delivery and therapeutics of canonical and Dicer-substrate siRNA
Raja, M. A. G., Katas, H., & Amjad, M. W. (2019) · Asian Journal of Pharmaceutical Sciences · doi:10.1016/j.ajps.2018.12.005 · Cited by 46 · see citing works
Intracellular Photothermal Delivery for Suspension Cells Using Sharp Nanoscale Tips in Microwells
Man, T., Zhu, X., Chow, Y. T., Dawson, E. R., Wen, X., Patananan, A. N., Liu, T. “., Zhao, C., Wu, C., Hong, J. S., et al. (2019) · ACS Nano · doi:10.1021/acsnano.9b06025 · Cited by 46 · see citing works
Cordelier, P., Morse, B. A., & Strayer, D. S. (2003) · Oligonucleotides · doi:10.1089/154545703322616961 · Cited by 46 · see citing works
RNAi knock-down mice: an emerging technology for post-genomic functional genetics
Prawitt, D., Brixel, L. R., Spangenberg, C., Eshkind, L. G., Heck, R., Oesch, F., Zabel, B., & Bockamp, E. (2004) · Cytogenetic and Genome Research · doi:10.1159/000078214 · Cited by 46 · see citing works
Raes, L., Hecke, C. V., Michiels, J., Stremersch, S., Fraire, J. C., Brans, T., Xiong, R., Smedt, S. C. D., Vandekerckhove, L., Raemdonck, K., et al. (2019) · Crystals · doi:10.3390/cryst9080411 · Cited by 45 · see citing works
Cyclic Peptide-Capped Gold Nanoparticles for Enhanced siRNA Delivery
Shirazi, A. N., Paquin, K. L., Howlett, N. G., Mandal, D., & Parang, K. (2014) · Molecules · doi:10.3390/molecules190913319 · Cited by 44 · see citing works
A novel cell-penetrating peptide TAT-A1 delivers siRNA into tumor cells selectively
Fang, B., Lu, J., Zhang, M., & Ren, F. (2012) · Biochimie · doi:10.1016/j.biochi.2012.09.020 · Cited by 44 · see citing works
RNA Therapeutics for Improving CAR T-cell Safety and Efficacy
Schaible, P., Bethge, W. A., Lengerke, C., & Haraszti, R. Á. (2022) · Cancer Research · doi:10.1158/0008-5472.can-22-2155 · Cited by 43 · see citing works
Keck, K., Volper, E. M., Spengler, R. M., Long, D., Chan, C. Y., Ding, Y., & McCaffrey, A. P. (2008) · Molecular Therapy · doi:10.1038/mt.2008.273 · Cited by 40 · see citing works
RNA interference: gene silencing in the fast lane
Kittler, R., & Buchholz, F. (2003) · Seminars in Cancer Biology · doi:10.1016/s1044-579x(03)00039-7 · Cited by 40 · see citing works
Non-Viral Transfection of Human T Lymphocytes
Riedl, S. A. B., Kaiser, P., Raup, A., Synatschke, C. V., Jérôme, V., & Freitag, R. (2018) · Processes · doi:10.3390/pr6100188 · Cited by 40 · see citing works
Saifullah, M., Fox, D. A., Sarkar, S., Abidi, S. M. A., Endres, J., Piktel, J. S., Haqqi, T. M., & Singer, N. G. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.10.6125 · Cited by 39 · see citing works
Nucleoside phosphocholine amphiphile for in vitro DNA transfection
Moreau, L., Barthélémy, P., Li, Y., Luo, D., Prata, C. A. H., & Grinstaff, M. W. (2005) · Molecular BioSystems · doi:10.1039/b503302k · Cited by 39 · see citing works
- Authors
- McManus, M. T., Haines, B. B., Dillon, C. P., Whitehurst, C. E., van Parijs, L., Chen, J., & Sharp, P. A.
- Affiliations
- Massachusetts Institute of Technology (US)
- Center for Cancer Research, Massachusetts Institute of Technology , Cambridge, MA 02139
- ORCID
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-11
- Volume
- 169
- Issue
- 10
- Pages
- 5754-5760
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W2157078151
- Cited by
- 215
- References
- 40
- Citations by year
- 2025: 1 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 5 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 6 · 2013: 2 · 2012: 14
- FWCI
- 9.97
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Cancer Research Institute
- Arthritis Foundation
- National Institutes of Health (CA42063)
- National Cancer Institute (P01CA042063, P01 CA 42063)
- National Institute of General Medical Sciences (R37-GM34277, R37GM034277)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- siRNA, RNA interference, gene silencing, T cells, CD4, CD8A, primary lymphocytes
- MeSH terms
- Animals, Luminescent Proteins, Membrane Glycoproteins, Mice, Inbred C57BL, Mice, Mutant Strains, Mice, Transgenic, RNA, Messenger, Spleen, T-Lymphocytes, Thymus Gland, Tumor Cells, Cultured, CD4 Antigens, CD8 Antigens, Gene Silencing, RNA Stability, RNA, Small Interfering, Green Fluorescent Proteins, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review