Cited by 555RNA Research and SplicingRNA and protein synthesis mechanismsCancer-related molecular mechanisms research

Protein–RNA interactions: new genomic technologies and perspectives

König, Julian, Zarnack, Kathi, Luscombe, Nicholas M., Ule, Jernej·2012·Nature Reviews Genetics

Abstract

RNA-binding proteins are key players in the regulation of gene expression. In this Progress article, we discuss state-of-the-art technologies that can be used to study individual RNA-binding proteins or large complexes such as the ribosome. We also describe how these approaches can be used to study interactions with different types of RNAs, including nascent transcripts, mRNAs, microRNAs and ribosomal RNAs, in order to investigate transcription, RNA processing and translation. Finally, we highlight current challenges in data analysis and the future steps that are needed to obtain a quantitative and high-resolution picture of protein-RNA interactions on a genome-wide scale.

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Crossref · 10.1038/nrg3141

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  • Whole genome analyses brought a new appreciation of the pervasiveness and regulatory reach of alternative splicing in metazoan organisms.

    “The advent of whole genome analyses10,12,188,189 brought a new appreciation of its pervasiveness and regulatory reach in metazoan organisms190,191.”

    From The neurogenetics of alternative splicing (2016) · cites this paper as [10]

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Authors
König, J., Zarnack, K., Luscombe, N. M., & Ule, J.
Affiliations
  • MRC Laboratory of Molecular Biology (GB)
  • European Bioinformatics Institute (GB)
Corresponding author
Ule, Jernej
Venue
Nature Reviews Genetics
Published
2012-01-18
Volume
13
Issue
2
Pages
77-83
ISSN
  • 1471-0056 (ISSN-L)
  • 1471-0064
OpenAlex
W2127982146
Cited by
555
References
46
Citations by year
2026: 8 · 2025: 15 · 2024: 24 · 2023: 17 · 2022: 20 · 2021: 27 · 2020: 27 · 2019: 29 · 2018: 43 · 2017: 59 · 2016: 69 · 2015: 63 · 2014: 64 · 2013: 58 · 2012: 32
FWCI
6.33
Type
journal article
Language
en
Update notices
erratum (2012-01-31)
Open access
closed
Locations
  • Nature Reviews Genetics (publishedVersion)
  • PubMed (publishedVersion)
  • UCL Discovery (University College London) (submittedVersion)
Funders
  • Wellcome Trust (089701)
  • Medical Research Council
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
Keywords
RNA-protein interactions, RNA-binding proteins, gene expression regulation, ribosome, nascent RNA, mRNA, microRNA, ribosomal RNA, transcription, RNA processing, translation (biology)
MeSH terms
Animals, Humans, Ribonucleoproteins, RNA, RNA-Binding Proteins, Computational Biology, Genomics, High-Throughput Nucleotide Sequencing

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