Protein–RNA interactions: new genomic technologies and perspectives
Abstract
RNA-binding proteins are key players in the regulation of gene expression. In this Progress article, we discuss state-of-the-art technologies that can be used to study individual RNA-binding proteins or large complexes such as the ribosome. We also describe how these approaches can be used to study interactions with different types of RNAs, including nascent transcripts, mRNAs, microRNAs and ribosomal RNAs, in order to investigate transcription, RNA processing and translation. Finally, we highlight current challenges in data analysis and the future steps that are needed to obtain a quantitative and high-resolution picture of protein-RNA interactions on a genome-wide scale.
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Whole genome analyses brought a new appreciation of the pervasiveness and regulatory reach of alternative splicing in metazoan organisms.
“The advent of whole genome analyses10,12,188,189 brought a new appreciation of its pervasiveness and regulatory reach in metazoan organisms190,191.”
From The neurogenetics of alternative splicing (2016) · cites this paper as [10]
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- Authors
- König, J., Zarnack, K., Luscombe, N. M., & Ule, J.
- Affiliations
- MRC Laboratory of Molecular Biology (GB)
- European Bioinformatics Institute (GB)
- Corresponding author
- Ule, Jernej
- Venue
- Nature Reviews Genetics
- Published
- 2012-01-18
- Volume
- 13
- Issue
- 2
- Pages
- 77-83
- ISSN
- 1471-0056 (ISSN-L)
- 1471-0064
- OpenAlex
- W2127982146
- Cited by
- 555
- References
- 46
- Citations by year
- 2026: 8 · 2025: 15 · 2024: 24 · 2023: 17 · 2022: 20 · 2021: 27 · 2020: 27 · 2019: 29 · 2018: 43 · 2017: 59 · 2016: 69 · 2015: 63 · 2014: 64 · 2013: 58 · 2012: 32
- FWCI
- 6.33
- Type
- journal article
- Language
- en
- Update notices
- erratum (2012-01-31)
- Open access
- closed
- Locations
- Nature Reviews Genetics (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Wellcome Trust (089701)
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- RNA-protein interactions, RNA-binding proteins, gene expression regulation, ribosome, nascent RNA, mRNA, microRNA, ribosomal RNA, transcription, RNA processing, translation (biology)
- MeSH terms
- Animals, Humans, Ribonucleoproteins, RNA, RNA-Binding Proteins, Computational Biology, Genomics, High-Throughput Nucleotide Sequencing
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