Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells
Open accessAbstract
Whereas the strong and stable suppression of specific microRNA activity would be essential for the functional analysis of these molecules, and also for the development of therapeutic applications, effective inhibitory methods to achieve this have not yet been fully established. In our current study, we tested various RNA decoys which were designed to efficiently expose indigestible complementary RNAs to a specific miRNA molecule. These inhibitory RNAs were at the same time designed to be expressed in lentiviral vectors and to be transported into the cytoplasm after transcription by RNA polymerase III. We report the optimal conditions that we have established for the design of such RNA decoys (we term these molecules TuD RNAs; tough decoy RNAs). We finally demonstrate that TuD RNAs induce specific and strong biological effects and also show that TuD RNAs achieve the efficient and long-term-suppression of specific miRNAs for over 1 month in mammalian cells.
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Sponge constructs are designed to bind complementary miRNA(s) or their seed sequences, and hence block the binding of the miRNA to its biological targets.
“The sponge construct are designed to bind its complementary miRNA(s) or their seed sequences, and hence block the binding of the miRNA to its biological targets (29–31).”
From Potential Therapeutic Effects of Exosomes Packed With a miR-21-Sponge Construct in a Rat Model of Glioblastoma (2019) · cites this paper as [31]
miRNA inhibition using decoy or sponge-like constructs has potential therapeutic benefit.
“It has already been shown that miRNA inhibition using decoy or sponge-like constructs have a potential therapeutic benefit.”
From Potential Therapeutic Effects of Exosomes Packed With a miR-21-Sponge Construct in a Rat Model of Glioblastoma (2019) · cites this paper as [31]
Using decoy or sponge-like constructs for miRNA inhibition has been shown to bind complementary miRNA(s) or their seed sequences and block their effects.
“Using decoy or sponge-like constructs for miRNA inhibition have been already shown before with good effect in binding to its complementary miRNA(s) or their seed sequences and blocking their effects (29–31, 56, 57).”
From Potential Therapeutic Effects of Exosomes Packed With a miR-21-Sponge Construct in a Rat Model of Glioblastoma (2019) · cites this paper as [31]
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- Authors
- Haraguchi, T., Ozaki, Y., & Iba, H.
- Affiliations
- Institute of Medical Science, The University of Tokyo (JP)
- Corresponding author
- Iba, Hideo
- ORCID
- Haraguchi, Takeshi
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-02-17
- Volume
- 37
- Issue
- 6
- Pages
- e43-e43
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC2665227
- OpenAlex
- W2128520557
- Cited by
- 305
- References
- 29
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 15 · 2023: 6 · 2022: 14 · 2021: 10 · 2020: 20 · 2019: 18 · 2018: 21 · 2017: 27 · 2016: 29 · 2015: 24 · 2014: 33 · 2013: 23 · 2012: 23
- FWCI
- 5.59
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nucleic Acids Research (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- microRNA inhibition, RNA decoys, lentiviral vectors, RNA polymerase III transcription, mammalian cells
- MeSH terms
- Binding Sites, Cell Line, Genetic Vectors, Humans, Nucleic Acid Conformation, Transduction, Genetic, Lentivirus, Genes, Reporter, RNA, Untranslated, MicroRNAs
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