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The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.

CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells

Open access
Wang, Ke, Chen, Wei, Zhang, Zheng, Deng, Yongqiang, Lian, Jian-Qi, Du, Peng, Wei, Ding, Zhang, Yang, Sun, Xiu-Xuan, Gong, Li, Yang, Xu, He, Lei, Zhang, Lei, Yang, Zhiwei, Geng, Jie-Jie, Chen, Ruo, Zhang, Hai, Wang, Bin, Zhu, Yu-Meng, Nan, Gang, Jiang, Jian-Li, Li, Ling, Wu, Jiao, Lin, Peng, Huang, Wan, Xie, Liangzhi, Zheng, Zhao-Hui, Zhang, Kui, Miao, Jin-Lin, Cui, Hong-Yong, Huang, Min, Zhang, Jun, Fu, Ling, Yang, Xiang-Min, Zhao, Zhongpeng, Sun, Shihui, Gu, Hongjing, Wang, Zhe, Wang, Chun-Fu, Lu, Yacheng, Liu, Ying-Ying, Wang, Qing-Yi, Bian, Huijie, Zhu, Ping, Chen, Zhi-Nan·2020·Signal Transduction and Targeted Therapy
DOI 10.1038/s41392-020-00426-x

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Abstract

In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2, no specific and effective drugs for treating this disease have been reported until today. Angiotensin-converting enzyme 2 (ACE2), a receptor of SARS-CoV-2, mediates the virus infection by binding to spike protein. Although ACE2 is expressed in the lung, kidney, and intestine, its expressing levels are rather low, especially in the lung. Considering the great infectivity of COVID-19, we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection. Here, we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein. The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody, Meplazumab, inhibits SARS-CoV-2 amplification. Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells, which can be neutralized by CD147 extracellular fragment. Viral loads are detectable in the lungs of human CD147 (hCD147) mice infected with SARS-CoV-2, but not in those of virus-infected wild type mice. Interestingly, virions are observed in lymphocytes of lung tissue from a COVID-19 patient. Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dose-dependent manner, which is specifically inhibited by Meplazumab. Furthermore, CD147 mediates virus entering host cells by endocytosis. Together, our study reveals a novel virus entry route, CD147-spike protein, which provides an important target for developing specific and effective drug against COVID-19.

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Metadata

Authors
Wang, K., Chen, W., Zhang, Z., Deng, Y., Lian, J.-Q., Du, P., Wei, D., Zhang, Y., Sun, X.-X., Gong, L., Yang, X., He, L., Zhang, L., Yang, Z., Geng, J.-J., Chen, R., Zhang, H., Wang, B., Zhu, Y.-M., Nan, G., Jiang, J.-L., Li, L., Wu, J., Lin, P., Huang, W., Xie, L., Zheng, Z.-H., Zhang, K., Miao, J.-L., Cui, H.-Y., Huang, M., Zhang, J., Fu, L., Yang, X.-M., Zhao, Z., Sun, S., Gu, H., Wang, Z., Wang, C.-F., Lu, Y., Liu, Y.-Y., Wang, Q.-Y., Bian, H., Zhu, P., & Chen, Z.-N.
Venue
Signal Transduction and Targeted Therapy
Publisher
Springer Science and Business Media LLC
Published
2020-12-04
Volume
5
Issue
1
Pages
283-283
ISSN
2059-3635 (ISSN-L)
DOI
10.1038/s41392-020-00426-x
OpenAlex
W3107235131
Cited by
1,593
References
12
FWCI
40.29
Type
journal article
Language
en
Open access
gold
Licence

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CoChat. (2026). Citation check: CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells. https://app.cochat.ai/papers/10.1038/s41392-020-00426-x
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Wang, K., Chen, W., Zhang, Z., Deng, Y., Lian, J.-Q., Du, P., Wei, D., Zhang, Y., Sun, X.-X., Gong, L., Yang, X., He, L., Zhang, L., Yang, Z., Geng, J.-J., Chen, R., Zhang, H., Wang, B., Zhu, Y.-M., . . . Chen, Z.-N. (2020). CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells. Signal Transduction and Targeted Therapy. https://doi.org/10.1038/s41392-020-00426-x

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