Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration
Open accessAbstract
The capacity of 3D organoids to mimic physiological tissue organization and functionality has provided an invaluable tool to model development and disease in vitro. However, conventional organoid cultures primarily represent the homeostasis of self-organizing stem cells and their derivatives. Here, we established a novel intestinal organoid culture system composed of 8 components, mainly including VPA, EPZ6438, LDN193189, and R-Spondin 1 conditioned medium, which mimics the gut epithelium regeneration that produces hyperplastic crypts following injury; therefore, these organoids were designated hyperplastic intestinal organoids (Hyper-organoids). Single-cell RNA sequencing identified different regenerative stem cell populations in our Hyper-organoids that shared molecular features with in vivo injury-responsive Lgr5 + stem cells or Clu + revival stem cells. Further analysis revealed that VPA and EPZ6438 were indispensable for epigenome reprogramming and regeneration in Hyper-organoids, which functioned through epigenetically regulating YAP signaling. Furthermore, VPA and EPZ6438 synergistically promoted regenerative response in gut upon damage in vivo. In summary, our results demonstrated a new in vitro organoid model to study epithelial regeneration, highlighting the importance of epigenetic reprogramming that pioneers tissue repair.
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Significant differences in morphology, functionality, or organoid formation efficiency depend on the cells-of-origin, different cells in the organoid, and medium composition.
“Significant differences in morphology and functionality or even organoid formation efficiency depend on the different cells-of-origin of the culture or different cells in the organoid as well as different medium composition120,202–204.”
From Organoids (2022) · cites this paper as [120]
In Results, the paper reports that 8C versus ENR medium changes intestinal organoid morphology and regenerative behavior, and that Lgr5-GFP+ cells have higher organoid-forming efficiency than Lgr5-GFP− cells under 8C. The lineage-composition Results show different cells in the organoids, but do not establish that those differences cause the claimed changes in morphology, functionality, or formation efficiency. (LLM, conf=0.84)
Experimental — accuracy not yet measuredFujii et al. demonstrated that refining culture conditions could increase the diversity of differentiated cell fates in human small intestinal organoids.
“In that regard, a nice example was described by Fujii M et al. who demonstrated how refining of culture conditions could increase the diversity of differentiated cell fates in human small intestinal organoids120.”
From Organoids (2022) · cites this paper as [120]
The Results section reports that organoids grown under the new 8C culture condition contained multiple differentiated intestinal lineages, and Materials and methods describes culture supplements used to induce specific lineages (page numbers unavailable). But this paper is by Qu et al., not Fujii et al.; the cited passages do not demonstrate increased diversity of differentiated fates in human small intestinal organoids. (LLM, conf=0.78)
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Cites
Fast and accurate short read alignment with Burrows-Wheeler transform
Li, H., & Durbin, R. (2009) · Bioinformatics · doi:10.1093/bioinformatics/btp324 · Cited by 58,607 · see citing works
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., et al. (2009) · Nature · doi:10.1038/nature07935 · Cited by 7,204 · see citing works
Identification of stem cells in small intestine and colon by marker gene Lgr5
Barker, N., van Es, J. H., Kuipers, J., Kujala, P., van den Born, M., Cozijnsen, M., Haegebarth, A., Korving, J., Begthel, H., Peters, P. J., et al. (2007) · Nature · doi:10.1038/nature06196 · Cited by 5,687 · see citing works
Modeling Development and Disease with Organoids
Clevers, H. (2016) · Cell · doi:10.1016/j.cell.2016.05.082 · Cited by 3,196 · see citing works
Organogenesis in a dish: Modeling development and disease using organoid technologies
Lancaster, M. A., & Knoblich, J. A. (2014) · Science · doi:10.1126/science.1247125 · Cited by 2,931 · see citing works
Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts
Sato, T., van Es, J. H., Snippert, H. J., Stange, D. E., Vries, R. G., van den Born, M., Barker, N., Shroyer, N. F., van de Wetering, M., & Clevers, H. (2011) · Nature · doi:10.1038/nature09637 · Cited by 2,510 · see citing works
Snippert, H. J., van der Flier, L. G., Sato, T., van Es, J. H., van den Born, M., Kroon-Veenboer, C., Barker, N., Klein, A. M., van Rheenen, J., Simons, B. D., et al. (2010) · Cell · doi:10.1016/j.cell.2010.09.016 · Cited by 1,951 · see citing works
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro
Spence, J. R., Mayhew, C. N., Rankin, S. A., Kuhar, M. F., Vallance, J. E., Tolle, K. A., Hoskins, E. E., Kalinichenko, V. V., Wells, S. I., Zorn, A. M., et al. (2010) · Nature · doi:10.1038/nature09691 · Cited by 1,911 · see citing works
Phiel, C. J., Zhang, F., Huang, E. Y., Guenther, M. G., Lazar, M. A., & Klein, P. S. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m101287200 · Cited by 1,758 · see citing works
A single-cell survey of the small intestinal epithelium
Haber, A. L., Biton, M., Rogel, N., Herbst, R. H., Shekhar, K., Smillie, C., Burgin, G., Delorey, T. M., Howitt, M. R., Katz, Y., et al. (2017) · Nature · doi:10.1038/nature24489 · Cited by 1,750 · see citing works
Regulation of the Hippo-YAP Pathway by G-Protein-Coupled Receptor Signaling
Yu, F.-X., Zhao, B., Panupinthu, N., Jewell, J. L., Lian, I., Wang, L. H., Zhao, J., Yuan, H., Tumaneng, K., Li, H., et al. (2012) · Cell · doi:10.1016/j.cell.2012.06.037 · Cited by 1,628 · see citing works
Chemically induced mouse models of acute and chronic intestinal inflammation
Wirtz, S., Popp, V., Kindermann, M., Gerlach, K., Weigmann, B., Fichtner-Feigl, S., & Neurath, M. F. (2017) · Nature Protocols · doi:10.1038/nprot.2017.044 · Cited by 1,575 · see citing works
Liu-Chittenden, Y., Huang, B., Shim, J. S., Chen, Q., Lee, S.-J., Anders, R. A., Liu, J. O., & Pan, D. (2012) · Genes & Development · doi:10.1101/gad.192856.112 · Cited by 1,452 · see citing works
Adult intestinal stem cells: critical drivers of epithelial homeostasis and regeneration
Barker, N. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3721 · Cited by 1,334 · see citing works
Coexistence of Quiescent and Active Adult Stem Cells in Mammals
Li, L., & Clevers, H. (2010) · Science · doi:10.1126/science.1180794 · Cited by 1,247 · see citing works
Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications
Sato, T., & Clevers, H. (2013) · Science · doi:10.1126/science.1234852 · Cited by 1,170 · see citing works
Tales from the crypt: new insights into intestinal stem cells
Gehart, H., & Clevers, H. C. (2018) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-018-0081-y · Cited by 984 · see citing works
Hippo–YAP/TAZ signalling in organ regeneration and regenerative medicine
Moya, I. M., & Halder, G. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0086-y · Cited by 981 · see citing works
Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche
Ootani, A., Li, X., Sangiorgi, E., Ho, Q. T., Ueno, H., Toda, S., Sugihara, H., Fujimoto, K., Weissman, I. L., Capecchi, M. R., et al. (2009) · Nature Medicine · doi:10.1038/nm.1951 · Cited by 906 · see citing works
Dextran Sodium Sulphate Colitis Mouse Model: Traps and Tricks
Perše, M., & Cerar, A. (2012) · BioMed Research International · doi:10.1155/2012/718617 · Cited by 886 · see citing works
Disease modeling in stem cell-derived 3D organoid systems
Dutta, D., Heo, I., & Clevers, H. (2017) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2017.02.007 · Cited by 838 · see citing works
The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations
Yan, K. S., Chia, L. A., Li, X., Ootani, A., Su, J., Lee, J. Y., Su, N. Y., Luo, Y., Heilshorn, S. C., Amieva, M. R., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1118857109 · Cited by 817 · see citing works
Knutson, S. K., Warholic, N. M., Wigle, T. J., Klaus, C. R., Allain, C. J., Raimondi, A., Scott, M. P., Chesworth, R., Moyer, M. P., Copeland, R. A., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1303800110 · Cited by 794 · see citing works
Dll1+ secretory progenitor cells revert to stem cells upon crypt damage
van Es, J. H., Sato, T., van de Wetering, M., Lyubimova, A., Nee, A. N. Y., Gregorieff, A., Sasaki, N., Zeinstra, L. M., van den Born, M., Korving, J., et al. (2012) · Nature Cell Biology · doi:10.1038/ncb2581 · Cited by 781 · see citing works
YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
Yui, S., Azzolin, L., Maimets, M., Pedersen, M. T., Fordham, R. P., Hansen, S. L., Larsen, H. L., Guiu, J., Alves, M. R. P., Rundsten, C. F., et al. (2017) · Cell stem cell · doi:10.1016/j.stem.2017.11.001 · Cited by 678 · see citing works
Yap-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer
Gregorieff, A., Liu, Y., Inanlou, M. R., Khomchuk, Y., & Wrana, J. L. (2015) · Nature · doi:10.1038/nature15382 · Cited by 647 · see citing works
Tetteh, P. W., Basak, O., Farin, H. F., Wiebrands, K., Kretzschmar, K., Begthel, H., van den Born, M., Korving, J., de Sauvage, F., van Es, J. H., et al. (2016) · Cell stem cell · doi:10.1016/j.stem.2016.01.001 · Cited by 575 · see citing works
Niche-independent high-purity cultures of Lgr5+ intestinal stem cells and their progeny
Yin, X., Farin, H. F., van Es, J. H., Clevers, H. C., Langer, R., & Karp, J. M. (2013) · Nature Methods · doi:10.1038/nmeth.2737 · Cited by 571 · see citing works
Single-cell transcriptomes of the regenerating intestine reveal a revival stem cell
Ayyaz, A., Kumar, S., Sangiorgi, B., Ghoshal, B., Gosio, J., Ouladan, S., Fink, M., Barutcu, S., Trcka, D., Shen, J., et al. (2019) · Nature · doi:10.1038/s41586-019-1154-y · Cited by 569 · see citing works
Self-citationModeling mouse and human development using organoid cultures
Huch, M., & Koo, B. (2015) · Development · doi:10.1242/dev.118570 · Cited by 543 · see citing works
Restriction of intestinal stem cell expansion and the regenerative response by YAP
Barry, E., Morikawa, T., Butler, B. L., Shrestha, K., de la Rosa, R., Yan, K. S., Fuchs, C. S., Magness, S. T., Smits, R., Ogino, S., et al. (2012) · Nature · doi:10.1038/nature11693 · Cited by 537 · see citing works
Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche
Nusse, Y. M., Savage, A. K., Marangoni, P., Huber, A. R., Landman, T. A., de Sauvage, F. J., Locksley, R. M., & Klein, O. D. (2018) · Nature · doi:10.1038/s41586-018-0257-1 · Cited by 333 · see citing works
Regulation and plasticity of intestinal stem cells during homeostasis and regeneration
Beumer, J., & Clevers, H. C. (2016) · Development · doi:10.1242/dev.133132 · Cited by 286 · see citing works
A rapid micro chromatin immunoprecipitation assay (ChIP)
Dahl, J. A., & Collas, P. (2008) · Nature Protocols · doi:10.1038/nprot.2008.68 · Cited by 284 · see citing works
Jadhav, U., Saxena, M., O’Neill, N. K., Saadatpour, A., Yuan, G., Herbert, Z. T., Murata, K., & Shivdasani, R. A. (2017) · Cell stem cell · doi:10.1016/j.stem.2017.05.001 · Cited by 283 · see citing works
Phenotypic landscape of intestinal organoid regeneration
Lukonin, I., Serra, D., Challet Meylan, L., Volkmann, K., Baaten, J., Zhao, R., Meeusen, S., Colman, K., Maurer, F., Stadler, M. B., et al. (2020) · Nature · doi:10.1038/s41586-020-2776-9 · Cited by 283 · see citing works
Paneth Cell Multipotency Induced by Notch Activation following Injury
Yu, S., Tong, K., Zhao, Y., Balasubramanian, I., Yap, G. S., Ferraris, R. P., Bonder, E. M., Verzi, M. P., & Gao, N. (2018) · Cell stem cell · doi:10.1016/j.stem.2018.05.002 · Cited by 281 · see citing works
Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells
Murata, K., Jadhav, U., Madha, S., van Es, J. H., Dean, J., Cavazza, A., Wucherpfennig, K. W., Franziska Michor, A., Clevers, H. C., & Shivdasani, R. A. (2020) · Cell stem cell · doi:10.1016/j.stem.2019.12.011 · Cited by 244 · see citing works
Long-Term Culture Captures Injury-Repair Cycles of Colonic Stem Cells
Wang, Y., Chiang, I.-L., Ohara, T. E., Fujii, S., Cheng, J., Muegge, B. D., Heul, A. V., Han, N. D., Lu, Q., Xiong, S., et al. (2019) · Cell · doi:10.1016/j.cell.2019.10.015 · Cited by 196 · see citing works
Defining Adult Stem Cell Function at Its Simplest: The Ability to Replace Lost Cells through Mitosis
Post, Y., & Clevers, H. C. (2019) · Cell stem cell · doi:10.1016/j.stem.2019.07.002 · Cited by 176 · see citing works
Diverse mechanisms for endogenous regeneration and repair in mammalian organs
Wells, J. M., & Watt, F. M. (2018) · Nature · doi:10.1038/s41586-018-0073-7 · Cited by 169 · see citing works
Advancing Intestinal Organoid Technology Toward Regenerative Medicine
Nakamura, T., & Sato, T. (2017) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2017.10.006 · Cited by 118 · see citing works
Ex vivo culture of the intestinal epithelium: strategies and applications
Leushacke, M., & Barker, N. (2014) · Gut · doi:10.1136/gutjnl-2014-307204 · Cited by 113 · see citing works
Tomić, G., Morrissey, E. R., Kozar, S., Ben‐Moshe, S., Hoyle, A., Azzarelli, R., Kemp, R., Chilamakuri, C. S. R., Itzkovitz, S., Philpott, A., et al. (2018) · Cell stem cell · doi:10.1016/j.stem.2018.07.002 · Cited by 107 · see citing works
Wnt activity and basal niche position sensitize intestinal stem and progenitor cells to DNA damage
Tao, S., Tang, D., Morita, Y., Sperka, T., Omrani, O., Lechel, A., Sakk, V., Kraus, J. M., Kestler, H. A., Kühl, M., et al. (2015) · The EMBO Journal · doi:10.15252/embj.201490700 · Cited by 95 · see citing works
Development and application of human adult stem or progenitor cell organoids
Rookmaaker, M. B., Schutgens, F., Verhaar, M. C., & Clevers, H. C. (2015) · Nature Reviews Nephrology · doi:10.1038/nrneph.2015.118 · Cited by 88 · see citing works
Hierarchy and Plasticity in the Intestinal Stem Cell Compartment
Yousefi, M., Li, L., & Lengner, C. J. (2017) · Trends in Cell Biology · doi:10.1016/j.tcb.2017.06.006 · Cited by 81 · see citing works
Chu, H., Xi, T., Sun, Z., Hao, W., & Wei, X. (2019) · Life Sciences · doi:10.1016/j.lfs.2019.117220 · Cited by 70 · see citing works
Catestatin decreases macrophage function in two mouse models of experimental colitis.
Rabbi, M. F., Labis, B., Metz-Boutigue, M., Bernstein, C., & Ghia, J. (2014) · Biochemical Pharmacology · doi:10.1016/j.bcp.2014.03.003 · Cited by 62 · see citing works
Martín, M. L., Adileh, M., Hsu, K., Hua, G., Lee, S. G., Li, C., Fuller, J. D., Rotolo, J. A., Bodo, S., Klingler, S., et al. (2019) · Cancer Research · doi:10.1158/0008-5472.can-19-0312 · Cited by 51 · see citing works
Self-citationMontenegro-Miranda, P. S., van der Meer, J. H. M., Jones, C. M., Meisner, S., Vermeulen, J. L. M., Köster, J., Wildenberg, M. E., Heijmans, J., Boudreau, F., Ribeiro, A., et al. (2020) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2020.02.007 · Cited by 39 · see citing works
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Zhao, Z., Chen, X., Dowbaj, A. M., Sljukic, A., Bratlie, K., Lin, L., Fong, E. L. S., Balachander, G. M., Chen, Z., Soragni, A., et al. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00174-y · Cited by 1,001 · see citing works
Kim, S., Min, S., Choi, Y. S., Jo, S.-H., Jung, J. H., Han, K., Kim, J., An, S., Ji, Y. W., Kim, Y.-G., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-29279-4 · Cited by 375 · see citing works
Mex3a marks drug-tolerant persister colorectal cancer cells that mediate relapse after chemotherapy
Álvarez-Varela, A., Novellasdemunt, L., Barriga, F. M., Hernando‐Momblona, X., Cañellas-Socias, A., Cano-Crespo, S., Sevillano, M., Cortina, C., Stork, D., Morral, C., et al. (2022) · Nature Cancer · doi:10.1038/s43018-022-00402-0 · Cited by 146 · see citing works
Organoids in gastrointestinal diseases: From experimental models to clinical translation
Günther, C., Winner, B., Neurath, M. F., & Stappenbeck, T. S. (2022) · Gut · doi:10.1136/gutjnl-2021-326560 · Cited by 145 · see citing works
Developments and Opportunities for 3D Bioprinted Organoids
Ya, R., Yang, X., Ma, Z., Sun, X., Zhang, Y., Li, W., Yang, H. S., Qiang, L., Yang, Z., Liu, Y., et al. (2021) · International Journal of Bioprinting · doi:10.18063/ijb.v7i3.364 · Cited by 129 · see citing works
TGFB1 induces fetal reprogramming and enhances intestinal regeneration
Chen, L., Qiu, X., Dupre, A., Pellón-Cárdenas, O., Fan, X., Xu, X., Rout, P., Walton, K. D., Burclaff, J., Zhang, R., et al. (2023) · Cell stem cell · doi:10.1016/j.stem.2023.09.015 · Cited by 116 · see citing works
Yao, Q., Cheng, S., Pan, Q., Yu, J., Cao, G., Li, L., & Cao, H. (2024) · MedComm · doi:10.1002/mco2.735 · Cited by 97 · see citing works
3d organ-on-a-chip: the convergence of microphysiological systems and organoids
Baptista, L. S., Porrini, C., Kronemberger, G. S., Kelly, D. J., & Perrault, C. M. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.1043117 · Cited by 90 · see citing works
Chemical reprogramming for cell fate manipulation: Methods, applications, and perspectives
Wang, J., Sun, S., & Deng, H. (2023) · Cell Stem Cell · doi:10.1016/j.stem.2023.08.001 · Cited by 91 · see citing works
Self-citationActivation of fetal-like molecular programs during regeneration in the intestine and beyond
Viragova, S., Li, D., & Klein, O. D. (2024) · Cell stem cell · doi:10.1016/j.stem.2024.05.009 · Cited by 88 · see citing works
Applications of human organoids in the personalized treatment for digestive diseases
Wang, Q., Guo, F., Jin, Y., & Ma, Y. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01194-6 · Cited by 82 · see citing works
Qian, M., Liu, J., Zhao, D., Cai, P., Pan, C., Jia, W., Gao, Y., Zhang, Y., Zhang, N., Zhang, Y., et al. (2021) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2021.08.014 · Cited by 69 · see citing works
The Hippo–YAP/TAZ Signaling Pathway in Intestinal Self-Renewal and Regeneration After Injury
Deng, F., Wu, Z., Zou, F., Wang, S., & Wang, X. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.894737 · Cited by 66 · see citing works
Mechanisms of radiation‐induced tissue damage and response
Zhou, L., Zhu, J., Liu, Y., Zhou, P., & Gu, Y. (2024) · MedComm · doi:10.1002/mco2.725 · Cited by 71 · see citing works
Current applications of intestinal organoids: a review
Xiang, T., Wang, J., & Li, H. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03768-3 · Cited by 62 · see citing works
Predicting Radiation-Induced Lung Injury in Patients With Lung Cancer: Challenges and Opportunities
Kuipers, M. E., Wink, K. C. J., Hiemstra, P. S., & Slats, A. M. (2023) · International Journal of Radiation Oncology*Biology*Physics · doi:10.1016/j.ijrobp.2023.10.044 · Cited by 55 · see citing works
Li, Y., Wang, X., Zhou, X., Chen, H., Song, S., Deng, L., Yao, Y., & Yin, X. (2025) · Nature Communications · doi:10.1038/s41467-024-55567-2 · Cited by 52 · see citing works
Pinto, S. F. T., Hosseini, M., Buckley, S. T., Yin, W., Garousi, J., Gräslund, T., van IJzendoorn, S., Santos, H. A., & Sarmento, B. F. C. C. (2024) · Journal of Controlled Release · doi:10.1016/j.jconrel.2024.01.015 · Cited by 48 · see citing works
Two-photon polymerization-based 3D micro-scaffolds toward biomedical devices
Wang, W., Chen, Z., Lin, B., Liu, M., Zhang, Y., Liu, S., Li, Y., & Zhao, Q. (2024) · Chemical Engineering Journal · doi:10.1016/j.cej.2024.152469 · Cited by 48 · see citing works
Advanced In Vitro Models for Preclinical Drug Safety: Recent Progress and Prospects
Nair, D. G., & Weiskirchen, R. (2024) · Current Issues in Molecular Biology · doi:10.3390/cimb47010007 · Cited by 45 · see citing works
Harnessing the power of artificial intelligence for human living organoid research
Wang, H., Li, X., You, X., & Zhao, G. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.08.027 · Cited by 43 · see citing works
From morphology to single-cell molecules: high-resolution 3D histology in biomedicine
Xu, X., Su, J., Zhu, R., Li, K., Zhao, X., Fan, J., & Mao, F. (2025) · Molecular Cancer · doi:10.1186/s12943-025-02240-x · Cited by 41 · see citing works
Epigenetic memory of colitis promotes tumour growth
Nagaraja, S., Ojeda-Miron, L., Zhang, R., Oreskovic, E., Hock, C., Yan, H., Zeve, D., Sharma, K., Hyman, R. R., Zhang, Q., et al. (2026) · Nature · doi:10.1038/s41586-026-10258-4 · Cited by 33 · see citing works
Intracellular pH dynamics regulates intestinal stem cell lineage specification
Liu, Y., Reyes, E. A., Castillo‐Azofeifa, D., Klein, O. D., Nystul, T. G., & Barber, D. L. (2023) · Nature Communications · doi:10.1038/s41467-023-39312-9 · Cited by 30 · see citing works
Organoids as preclinical models of human disease: progress and applications
Chen, B., Du, C., Wang, M., Guo, J., & Liu, X. (2024) · Medical Review · doi:10.1515/mr-2023-0047 · Cited by 30 · see citing works
Parente, I. A., Chiara, L., & Bertoni, S. (2024) · Life Sciences · doi:10.1016/j.lfs.2024.122875 · Cited by 29 · see citing works
Nanomaterials in Organoids: From Interactions to Personalized Medicine
Shi, Y., Han, X., Zou, S., & Liu, G. (2024) · ACS Nano · doi:10.1021/acsnano.4c13330 · Cited by 30 · see citing works
Genetic and epigenetic alterations in aging and rejuvenation of human
Park, K., Jeon, M. C., Lee, D., Kim, J., & Im, S.-W. (2024) · Molecules and Cells · doi:10.1016/j.mocell.2024.100137 · Cited by 28 · see citing works
Engineered Human Organoids for Biomedical Applications
Zhu, Y., Sun, L., Wu, X., Gu, Z., & Zhao, Y. (2023) · Advanced Functional Materials · doi:10.1002/adfm.202310961 · Cited by 27 · see citing works
Theranostic Lipid Nanoparticles for Renal Cell Carcinoma
Mao, X., Wang, G., Wang, Z., Duan, C., Wu, X., & Xu, H. (2023) · Advanced Materials · doi:10.1002/adma.202306246 · Cited by 27 · see citing works
Listen to Your Gut: Key Concepts for Bioengineering Advanced Models of the Intestine
Cameron, O., Neves, J. F., & Gentleman, E. (2023) · Advanced Science · doi:10.1002/advs.202302165 · Cited by 25 · see citing works
Eskiocak, O., Gewolb, J., Shah, V., Rouse, J. A., Chowdhury, S., Akyildiz, E. O., Fernández-Maestre, I., Boyer, J. A., Filliol, A. A., Harris, A. S., et al. (2025) · Nature Aging · doi:10.1038/s43587-025-01022-w · Cited by 27 · see citing works
Qian, S., Mao, J., Liu, Z., Zhao, B., Zhao, Q., Lu, B., Zhang, L., Mao, X., Cheng, L., Cui, W., et al. (2022) · Smart Medicine · doi:10.1002/smmd.20220007 · Cited by 25 · see citing works
Kang, T. H., & Lee, S. I. (2024) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-023-00976-4 · Cited by 25 · see citing works
Lu, J. N., Křepelová, A., Rasa, S. M. M., Annunziata, F., Husak, O., Adam, L., Nunna, S., & Neri, F. (2021) · Scientific Reports · doi:10.1038/s41598-021-96321-8 · Cited by 25 · see citing works
Domene, A., Orozco, H., Rodríguez-Viso, P., Monedero, V., Zúñiga, M., Vélez, D., & Devesa, V. (2023) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2023.110404 · Cited by 22 · see citing works
A patient-derived organoid model captures fetal-like plasticity in colorectal cancer
Xiong, L., Xu, Y., Gao, Z.-Y., Shi, J., Wang, Y.-F., Wang, X.-D., Huang, W.-S., Li, M., Wang, L.-T., Xu, J., et al. (2025) · Cell Research · doi:10.1038/s41422-025-01139-y · Cited by 18 · see citing works
Self-citationHuman gut homeostasis and regeneration: the role of the gut microbiota and its metabolites
Gómez, C. M. A., García-Gutiérrez, E., Escobar, J. S., & Cotter, P. D. (2022) · Critical Reviews in Microbiology · doi:10.1080/1040841x.2022.2142088 · Cited by 23 · see citing works
Kang, W., Jin, T., Zhāng, T., Ma, S., Yan, H., Liu, Z., Ji, Z., Cai, Y., Wang, S., Song, M., et al. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab870 · Cited by 19 · see citing works
Biomaterials for inflammatory bowel disease: treatment, diagnosis and organoids
Wang, J., Shi, Y., Mao, B., Zhang, B., Yang, J., Hu, Z., & Liao, W. (2024) · Applied Materials Today · doi:10.1016/j.apmt.2024.102078 · Cited by 19 · see citing works
Vitamin B12 ameliorates gut epithelial injury via modulating the HIF-1 pathway and gut microbiota
Feng, C., Yan, J., Luo, T., Zhang, H., Zhang, H., Yu, Y., Chen, Y., & Chen, H. (2024) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-024-05435-5 · Cited by 17 · see citing works
Research progress and application of liver organoids for disease modeling and regenerative therapy
Hu, Y., Geng, Q., Wang, L., Wang, Y., Huang, C., Fan, Z., & Kong, D. (2024) · Journal of Molecular Medicine · doi:10.1007/s00109-024-02455-3 · Cited by 18 · see citing works
Guo, W., Zhang, X., Li, L., Shao, P., Liang, C., Zhang, H., Liu, K., Wang, S., Peng, Y., Luo, J., et al. (2024) · Nature Genetics · doi:10.1038/s41588-024-01979-1 · Cited by 17 · see citing works
Self-citationIntestinal transit-amplifying cells require METTL3 for growth factor signaling and cell survival
Danan, C. H., Naughton, K. E., Hayer, K. E., Vellappan, S., McMillan, E. A., Zhou, Y., Matsuda, R., Nettleford, S. K., Katada, K., Parham, L. R., et al. (2023) · JCI Insight · doi:10.1172/jci.insight.171657 · Cited by 16 · see citing works
Progress and perspective of organoid technology in cancer-related translational medicine
Lin, Y., Jiang, L., He, Q., Yuan, M., & Cao, J. (2022) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2022.112869 · Cited by 15 · see citing works
Mechanisms and modeling of wound repair in the intestinal epithelium
Boger, K. D., Sheridan, A. E., Ziegler, A. L., & Blikslager, A. T. (2022) · Tissue Barriers · doi:10.1080/21688370.2022.2087454 · Cited by 15 · see citing works
Dietary nutrition regulates intestinal stem cell homeostasis
Ma, N., Chen, X., Liu, C., Sun, Y., Johnston, L. J., & Ma, X. (2022) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2022.2087052 · Cited by 15 · see citing works
Hertwig's epithelial root sheath cells show potential for periodontal complex regeneration
Bi, F., Tang, H., Zhang, Z., Lyu, Y., Huo, F., Chen, G., & Guo, W. (2022) · Journal of Periodontology · doi:10.1002/jper.22-0072 · Cited by 15 · see citing works
Self-citationBrown, R. E., Jacobse, J., Anant, S. A., Blunt, K. M., Chen, B., Vega, P. N., Jones, C. T., Pilat, J. M., Revetta, F. L., Gorby, A. H., et al. (2022) · JCI Insight · doi:10.1172/jci.insight.153045 · Cited by 15 · see citing works
Intestinal organoid modeling: bridging the gap from experimental model to clinical translation
Liu, T., Li, X., Li, H., Qin, J., Xu, H., Wen, J., Ya-qin, H., & Cao, Z. (2024) · Frontiers in Oncology · doi:10.3389/fonc.2024.1334631 · Cited by 16 · see citing works
Inhibition of EZH2 Reduces Aging-Related Decline in Interstitial Cells of Cajal of the Mouse Stomach
Taheri, N., Choi, E. L., Nguyen, V. T. T., Zhang, Y., Huynh, N. M., Kellogg, T. A., van Wijnen, A. J., Ördög, T., & Hayashi, Y. (2024) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2024.101376 · Cited by 14 · see citing works
Kumar, D., Gupta, S., Gupta, V., Tanwar, R., & Chandel, A. (2025) · Stem Cell Reviews and Reports · doi:10.1007/s12015-025-10893-w · Cited by 17 · see citing works
Dynamics and plasticity of stem cells in the regenerating human colonic epitheliumPreprint
Oost, K. C., Kahnwald, M., Barbiero, S., de Medeiros, G., Suppinger, S., Kalck, V., Meylan, L. C., Smallwood, S. A., Stadler, M. B., & Liberali, P. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.12.18.572103 · Cited by 13 · see citing works
Huang, W., Zhou, X., Fu, G., Ding, M., Wu, H., Zeng, M., Zhang, H., Xu, L., Gao, Y., Wang, H., et al. (2021) · Theranostics · doi:10.7150/thno.54457 · Cited by 13 · see citing works
Zhang, S., Sui, Y., Yan, S., Zhang, Y., Ding, C., Su, X., Xiong, J., & Wei, S. (2022) · Stem Cell Research & Therapy · doi:10.1186/s13287-022-03033-5 · Cited by 13 · see citing works
Establishment of Epithelial Inflammatory Injury Model Using Intestinal Organoid Cultures
Xing, C., Liang, G., Yu, X. H., Zhang, A., Luo, X., Liu, Y., Tang, Z., Wu, B., Song, Z., & Lan, D. (2023) · Stem Cells International · doi:10.1155/2023/3328655 · Cited by 12 · see citing works
Organoids in gastrointestinal diseases: from bench to clinic
Wang, Q., Guo, F.-Y., Zhang, Q., Hu, T., Jin, Y.-T., Yang, Y.-Z., & Ma, Y.-L. (2024) · MedComm · doi:10.1002/mco2.574 · Cited by 15 · see citing works
Modulation of β-Catenin promotes WNT expression in macrophages and mitigates intestinal injury
Chugh, R. M., Bhanja, P., Zitter, R., Gunewardena, S., Badkul, R., & Saha, S. (2025) · Cell Communication and Signaling · doi:10.1186/s12964-025-02065-7 · Cited by 12 · see citing works
Advances and applications of gut organoids: modeling intestinal diseases and therapeutic development
Xu, X., Zhang, Y., Huang, G.-X., Perekatt, A., Wang, Y., & Chen, L. (2025) · Life Medicine · doi:10.1093/lifemedi/lnaf012 · Cited by 14 · see citing works
Roca, M. S., Lombardi, R., Testa, C., Iannelli, F., Grumetti, L., Moccia, T., Barile, V., Addi, L., Memoli, D., Leone, A., et al. (2025) · Cell Death and Disease · doi:10.1038/s41419-025-07902-8 · Cited by 11 · see citing works
Wang, X., Zhong, Y., Zheng, C., Huang, C., Yao, H., Guo, Z., Wu, Y., Wang, Z., Wu, Z., Ge, R., et al. (2024) · Food Research International · doi:10.1016/j.foodres.2024.114999 · Cited by 11 · see citing works
Urological cancer organoids, patients' avatars for precision medicine: past, present and future
Chen, H., Zhang, W., Maskey, N., Yang, F., Zheng, Z., Li, C., Wang, R., Wu, P., Mao, S., Zhang, J., et al. (2022) · Cell & Bioscience · doi:10.1186/s13578-022-00866-8 · Cited by 12 · see citing works
Self-citationColorectal Organoids: Models, Imaging, Omics, Therapy, Immunology, and Ethics
Taglieri, M., Gregorio, L. D., Matis, S., Uras, C. R. M., Ardy, M., Casati, S., Marchese, M., Poggi, A. D., Raffaghello, L., & Benelli, R. (2025) · Cells · doi:10.3390/cells14060457 · Cited by 11 · see citing works
van der Net, M. C., Vliem, M. J., Kemp, L. J. S., Gonzalez, C. P., Haddad, T. S., Strating, E., García, A. K., Houtekamer, R. M., Pannekoek, W., van den Anker, K. B., et al. (2025) · Cell Reports · doi:10.1016/j.celrep.2025.116308 · Cited by 14 · see citing works
Advanced Progression for the Heterogeneity and Homeostasis of Intestinal Stem Cells
Sun, M., Tan, Z., Lin, K., Li, X., Zhu, J., Zhan, L., & Zheng, H. (2023) · Stem Cell Reviews and Reports · doi:10.1007/s12015-023-10578-2 · Cited by 10 · see citing works
Taking a Step Back: Insights into the Mechanisms Regulating Gut Epithelial Dedifferentiation
Ouladan, S., & Gregorieff, A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22137043 · Cited by 9 · see citing works
Yu, W., Lin, Y., Lü, Y., Wang, Y., Zhang, D., Quan, H., Luo, Y., Zhang, Y., Jiang, Z., Chen, J., et al. (2024) · Food Frontiers · doi:10.1002/fft2.408 · Cited by 9 · see citing works
Martinet, M. G., Thomas, M., Bojunga, J., Pletz, M. W., Vehreschild, M. J. G. T., & Würstle, S. M. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1553979 · Cited by 9 · see citing works
In vivo detection of endogenous toxic phenolic compounds of intestine.
Jin, W.-Y., Guo, J.-X., Tang, R., Wang, J.-L., Zhao, H., Zhang, M., Teng, L.-Z., Sansonetti, P., & Gao, Y.-Z. (2024) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2024.135526 · Cited by 7 · see citing works
Regulation of revival stem cell differentiation by CREPT/RPRD1B during intestinal regeneration
Lan, T., Li, M., Duan, X., Jia, H., Cao, Y., Wang, Y., Ren, F., Sheng, J., Xu, J., & Chang, Z. (2025) · Cell & Bioscience · doi:10.1186/s13578-025-01434-6 · Cited by 8 · see citing works
Self-citationOrgan‐Specific Dedifferentiation and Epigenetic Remodeling in In Vivo Reprogramming
Jo, B., Lee, S., Eom, H., Kim, J., & Cha, H. (2025) · Aging Cell · doi:10.1111/acel.70268 · Cited by 8 · see citing works
You, M., & Xu, M. (2024) · Nutrients · doi:10.3390/nu16060845 · Cited by 8 · see citing works
CL429 enhances the renewal of intestinal stem cells by upregulating TLR2-YAP1
Fang, L., Cheng, Y., Fang, D., Feng, Z., Wang, Y., Yu, Y., Zhao, J., Huang, D., Zhai, X., Liu, C., et al. (2024) · International Immunopharmacology · doi:10.1016/j.intimp.2024.112614 · Cited by 8 · see citing works
Intestinal organoid technology and applications in probiotics
Tan, H., Chen, X., Wang, C., Song, J., Xu, J., Zhang, Y., & Suo, H. (2023) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2023.2288887 · Cited by 8 · see citing works
Self-citationAdvanced analysis and applications of single-cell transcriptome sequencing
Zhao, R.-H., Yicheng, B., Jingying, Z., Hu, M., Xinyan, Z., Yang, M., Tengfei, D., & Junjing, J. (2023) · All Life · doi:10.1080/26895293.2023.2199140 · Cited by 7 · see citing works
Ren, J., Liu, M., Rong, M., Zhang, X., Wang, G., Liu, Y., Li, H., & Duan, S. (2024) · Cell organoid (Print) · doi:10.26599/co.2024.9410009 · Cited by 8 · see citing works
Self-citationChen, L., Hao, Y., Zhai, T., Yang, F., Chen, S., Lin, X., & Li, J. (2025) · Cancer Informatics · doi:10.1177/11769351251323569 · Cited by 6 · see citing works
Xia, M., Wu, G., Wu, D., Hu, W., Deng, H., & Wang, S. (2026) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2026.03.013 · Cited by 7 · see citing works
Self-citationOrganoids to Remodel SARS-CoV-2 Research: Updates, Limitations and Perspectives
An, Y., He, Y., Ge, N., Guo, J., Yang, F., & Sun, S. (2023) · Aging and Disease · doi:10.14336/ad.2023.0209 · Cited by 6 · see citing works
Zheng, S., Su, Y.-Y., Cai, F.-L., Xu, D.-F., & Xu, Y. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1669482 · Cited by 5 · see citing works
Xu, Z.-Y., Wang, M., Shi, J.-Y., Liu, Y., Yu, C., Zhang, X.-Y., Zhang, C.-W., He, Q.-F., Pan, C., Zhou, J., et al. (2025) · Materials Today Bio · doi:10.1016/j.mtbio.2025.101700 · Cited by 5 · see citing works
Self-citationNagao, I., Nakazawa, M., & Ambrosini, Y. M. (2024) · Journal of Visualized Experiments · doi:10.3791/65720 · Cited by 5 · see citing works
Zhang, Y., Yang, N., Li, Q., Tang, Y., Bai, B., & Liu, G. (2025) · Virology · doi:10.1016/j.virol.2025.110595 · Cited by 4 · see citing works
Single-cell genomic profiling to study regeneration
Maynard, A., Soretić, M., & Treutlein, B. (2024) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2024.102231 · Cited by 4 · see citing works
Wang, X., Jing, Y., Zheng, C., Huang, C., Yao, H., Guo, Z., Wu, Y., Wang, Z., Wu, Z., Ge, R., et al. (2024) · Food & Function · doi:10.1039/d4fo01723d · Cited by 4 · see citing works
Wang, J., Wang, T., Zhu, L., Wang, J., Gao, Q., Guo, L., Lv, G., Zhang, W., Zhang, Z., Yang, C., et al. (2025) · International Immunopharmacology · doi:10.1016/j.intimp.2025.114573 · Cited by 4 · see citing works
Construction of Lymphoma Organoids and Applications in Tumor Microenvironment Research
Zhu, M., Li, T., Han, S., Zhao, D., Chen, X., Wong, V., Wang, H., & Liu, Y. (2025) · European Journal Of Haematology · doi:10.1111/ejh.14448 · Cited by 4 · see citing works
Kim, H. M., Kim, Y., Kim, Y., Kim, Y. J., & Ko, K. S. (2023) · Preventive Nutrition and Food Science · doi:10.3746/pnf.2023.28.3.360 · Cited by 4 · see citing works
Applications and perspectives of tumor organoids in radiobiology (Review)
Jin, Y., Wang, K., Tang, Y., & Zheng, D. (2024) · Oncology Reports · doi:10.3892/or.2024.8759 · Cited by 4 · see citing works
Intestinal LKB1 Loss Drives a Premalignant Program Along the Serrated Cancer Pathway
Plugge, S., Ma, H., van der Vaart, J., Sprangers, J., Morsink, F. H. M., Xanthakis, D., Jamieson, C., Stroot, K. B. W., Keijzer, A., Margaritis, T., et al. (2025) · Gastroenterology · doi:10.1053/j.gastro.2025.07.041 · Cited by 6 · see citing works
A Three-Step Protocol to Differentiate iPSC into Colon Organoids
Aksoy, Z. B., & Akçalı, K. C. (2024) · Methods in molecular biology · doi:10.1007/978-1-0716-3995-5_6 · Cited by 3 · see citing works
Advances in the Study of Pluripotent Stem Cells in Livestock
Zhou, X., Gao, C., Zhao, W., Wei, X., Yu, D., Zou, H., & Du, W. (2025) · Cell Proliferation · doi:10.1111/cpr.70008 · Cited by 3 · see citing works
Wang, X., Zhang, W., Yang, S., Zheng, C., Wang, Z., Yang, G., Huang, C., Yao, H., Huang, K., Yan, Y., et al. (2026) · npj Science of Food · doi:10.1038/s41538-025-00672-9 · Cited by 3 · see citing works
Bai, J., Zhang, C., Li, G., Wang, Y., Dong, Y., Klar, R. M., & He, T. (2025) · Journal of Orthopaedic Translation · doi:10.1016/j.jot.2025.04.002 · Cited by 3 · see citing works
A Strainer-Based Platform for the Collection and Immunolabeling of Mouse Intestinal Organoids
Tan, J., Liu, Y., Li, W., Chen, G., Fang, Y., He, X., Fu, B., & Jing, Z. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241713568 · Cited by 3 · see citing works
Unraveling Interactions Between Gut Epithelium and Microbiota Using Models of Intestinal Organoids
Huang, Y., He, K., & Chen, Y. (2024) · Infectious Microbes & Diseases · doi:10.1097/im9.0000000000000153 · Cited by 3 · see citing works
Zeng, X., Yin, L., Zhang, Y., Wang, Q., Li, J., Yin, Y., Wang, Q., Li, J., & Yang, H. (2025) · Biological Trace Element Research · doi:10.1007/s12011-025-04546-9 · Cited by 2 · see citing works
Hu, X., Xie, Y., Wang, J., Zhang, X., & Wu, R. (2026) · Animals · doi:10.3390/ani16030469 · Cited by 2 · see citing works
Self-citationHepatitis E virus replication is maintained in proliferative cells within the intestinal crypt
Prallet, S., Maier, N., Li, A., Afting, C., Huang, H.-E., Hu, J., Toprak, E., Keya, D. P., Colasanti, O., León-Janampa, N., et al. (2026) · Science Advances · doi:10.1126/sciadv.aeb2333 · Cited by 2 · see citing works
Cellular mechano-memory informs biomaterials and mechano-medicine
Qiao, P., Zhao, H., Ma, Z., Hasani, M., & Song, Y. (2026) · Cell Reports Physical Science · doi:10.1016/j.xcrp.2026.103308 · Cited by 2 · see citing works
- Authors
- Qu, M., Xiong, L., Lyu, Y., Zhang, X., Shen, J., Guan, J., Chai, P., Lin, Z., Nie, B., Li, C., Xu, J., & Deng, H.
- Affiliations
- Peking University (CN)
- Capital Medical University (CN)
- Corresponding author
- Li, Cheng
- Xu, Jun
- Deng, Hongkui
- ORCID
- Venue
- Cell Research
- Published
- 2021-01-08
- Volume
- 31
- Issue
- 3
- Pages
- 259-271
- ISSN
- 1001-0602 (ISSN-L)
- 1748-7838
- PMC
- PMC8027647
- OpenAlex
- W3119003316
- Cited by
- 138
- References
- 51
- Citations by year
- 2026: 21 · 2025: 31 · 2024: 41 · 2023: 18 · 2022: 21 · 2021: 6
- FWCI
- 6.83
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Cell Research (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Greater South Information System
- Funders
- National Natural Science Foundation of China (31521004, 31871266, 2017YFA0103000, 31730059)
- Peking University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Digestive system and related health
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Keywords
- intestinal organoids, epithelial regeneration, intestinal injury, valproic acid, LDN-193189, RSPO1, Lgr5+ stem cells, epigenetic reprogramming, YAP signaling, tissue repair, single-cell RNA sequencing
- MeSH terms
- Animals, Benzamides, Biphenyl Compounds, Cells, Cultured, Colitis, Disease Models, Animal, Female, Intestinal Mucosa, Intestines, Male, Mice, Inbred C57BL, Mice, Transgenic, Morpholines, Organoids, Pyridones, Radiation Injuries, Regeneration, Stem Cells, Valproic Acid, Signal Transduction, Dextran Sulfate, Treatment Outcome, Culture Media, Conditioned, Tissue Culture Techniques, Mice
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