Cited by 346CRISPR and Genetic EngineeringVirus-based gene therapy researchRNA Interference and Gene Delivery

The DOI matches its Crossref record, with a small difference in title or year; 56 of the 56 references it cites were found in a registry or an index.

Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins

Open access

Mangeot, Philippe E., Risson, Valérie, Fusil, Floriane, Marnef, Aline, Laurent, Emilie, Blin, Juliana, Mournetas, Virginie, Massouridès, Emmanuelle, Sohier, Thibault J. M., Corbin, Antoine, Aubé, Fabien, Teixeira, Marie, Pinset, Christian, Schaeffer, Laurent, Legube, Gaëlle, Cosset, François-Loïc, Verhoeyen, Els, Ohlmann, Théophile, Ricci, Emiliano P.·2019·Nature Communications

DOI 10.1038/s41467-018-07845-zPMC PMC6318322

Published version of a preprint

Open this paper in CoChat — free account Open PDF

Abstract

Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells and living organisms. However, their delivery into target cells can be technically challenging when working with primary cells or in vivo. Here, we use engineered murine leukemia virus-like particles loaded with Cas9-sgRNA ribonucleoproteins (Nanoblades) to induce efficient genome-editing in cell lines and primary cells including human induced pluripotent stem cells, human hematopoietic stem cells and mouse bone-marrow cells. Transgene-free Nanoblades are also capable of in vivo genome-editing in mouse embryos and in the liver of injected mice. Nanoblades can be complexed with donor DNA for “all-in-one” homology-directed repair or programmed with modified Cas9 variants to mediate transcriptional up-regulation of target genes. Nanoblades preparation process is simple, relatively inexpensive and can be easily implemented in any laboratory equipped for cellular biology.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/s41467-018-07845-z

Cites

56 of 56 references verified0 citation errors · 0 not checkable

Statistics

Fig. 1 Nanoblade-mediated genome editing. a Scheme describing the MLV Gag::Cas9 fusion and the Nanoblade production protocol based on the transfection of HEK-293T cells by plasmids coding for Gag-Pol, Gag::Cas9, VSV-G, BaEVRLess, and the sgRNA. b Top panel, immunofluorescence analysis of γ-H2AX (green), RNA polI (red) in U2OS cells 8 h after being transduced with control Nanoblades or with Nanoblades targeting ribosomal DNA genes. Bottom panel, quantification of γ-H2AX and RNA polI colocalization foci in U2OS cells at different times after Nanoblades transduction or after classical DNA transfection methods (n = 3, error bars correspond to standard deviation). c Dose response of Nanoblades. HEK-293T cells were transduced with increasing amounts of Nanoblades targeting human EMX1 (n = 1 displayed). The exact amount of Cas9 used for transduction was measured by dot blot (in gray). Genome editing was assessed by Sanger sequencing and Tide analysis (in red)CC BY · PubMed Central

Fig. 2 Genome editing in primary cells transduced with Nanoblades. a Left panel, editing efficiency at the EMX1 locus (measured by high-throughput sequencing on the Illumina Miseq platform) of human-induced pluripotent stem cells (hiPSCs) transduced with Nanoblades targeting human EMX1 (n = 3). Right panel, expression of pluripotency markers measured by qPCR in control cells and cells transduced with Nanoblades targeting EMX1 (n = 3). b Left and middle panels, fluorescence microscopy and FACS analysis of GFP expressing BMDMs transduced at the bone marrow stage (day 0 after bone marrow collection) with control Nanoblades or Nanoblades targeting the GFP-coding sequence (n = 3). Right top panel, T7 endonuclease assay against the GFP sequence from Nanoblades-treated BMDMs. Right bottom panel, cytokine expression levels (measured by qPCR) in untreated or Nanoblade-treated cells upon LPS stimulation (n = 4). c T7 endonuclease assay against mouse Fto or human EMX1 genomic sequences amplified by PCR from primary mouse bone marrow cells transduced with Nanoblades or electroporated with recombinant Cas9-sgRNA RNPs. For bone marrow cells, two electroporation settings were tested. Lanes numbered #1–#3 correspond to biological replicates. Editing efficiencies were calculated by TIDE13 analysis of the Sanger sequencing electropherograms for each PCR amplicon d Left panel, excision of a 160 bp DNA fragment of MYD88 using Nanoblades. Middle panel PCR results obtained in human primary hepatocytes transduced with Nanoblades. Right-panel (top), FACS analysis of CD34+ cells purified from human cord-blood. Bottom, genome editing at the MYD88 locus assessed by PCR in untreated and Nanoblades-treated CD34+ cells. Error bars in all figures correspond to standard deviationCC BY · PubMed Central

Fig. 3 “All-in-one” Nanoblades for knock-in experiments and assessment of Nanoblades off-target activity. a Left panel, Nanoblades targeting human DDX3 close to its start codon were complexed with a donor ssDNA bearing homology arms to the targeted locus and a Flag-tag sequence in the presence of polybrene. HEK293T cells were then transduced with these “All-in-one” Nanoblades. After cell amplification, a fraction of cells were collected to extract genomic DNA and total proteins while the remaining cells were cultured to obtain single-cell clonal populations. Right panel, insertion of the Flag-tag in HEK-293T cells transduced with “all-in-one” Nanoblades complexed with increasing amounts of donor ssDNA was assessed by Flag-immunoprecipitation followed by western-blot using anti-flag or anti-DDX3 antibodies in the input and Flag-immunoprecipitation elution fractions. Flag insertion was also assessed by PCR using a forward primer in the flag-sequence and a reverse primer in the DDX3 locus (Orientation PCR assay) or using primers flanking the Flag sequence (Insertion PCR assay). Bottom panel, Flag-insertion in 20 different single-cell-derived clones was assessed by PCR using primers flanking the Flag-sequence. b Left panel, off-target monitoring in immortalized mouse macrophages stably expressing GFP transgenes bearing silent mutations in the region targeted by the sgRNA. Right panel, cells were transfected with plasmids coding for Cas9 and the sgRNA or transduced with Nanoblades. GFP expression was measured by FACS 72 h after transfection/transduction (n = 3). c Left and right panels, gene-editing at the EMX1 on-target site and the MFAP1 intronic off-target site measured by high-throughput sequencing in untreated cells (control cells) and cells transduced with EMX1 Nanoblades (Nanoblades) or transfected with plasmids coding for Cas9 and the EMX1 sgRNA (DNA transfection) (n = 3). Statistical significance of the Nanoblades and DNA transfection comparison at the on-target site was computed using a two-tail Student test. d Left panel, position of sgRNAs targeting the promoter of TTN and VLPs with different combination of sgRNAs produced for the experiment. Right-panel, TTN mRNA expression levels (normalized to Control) as measured by qPCR in MCF7 transduced with VLPs (n = 3). Error bars in all figures correspond to standard deviationCC BY · PubMed Central

Fig. 4 Generation of transgenic mice using Nanoblades. a Left panel, scheme describing injection of mCherry VLPs or Nanoblades in the perivitelline space of mouse 1-cell embryos. Right panel, fluorescence microscopy of mouse blastocysts injected with mCherry VLPs at the single-cell stage. b Scheme of the design strategy to target the mouse Tyr locus (adapted from ref. 22). Upon editing and NHEJ repair, the HinfI restriction site becomes inactive. c Survival rates of injected embryos at two-cell, blastocyst, and newborn stage (the latter obtained from experiments presented in Supplementary figure 5). d T7 endonuclease (top panel) and HinfI restrictions (bottom panel) assays on PCR fragments amplified from the Tyr locus of Control or Nanoblades-injected embryos. e Top left panel, photographs of F0 mice generated from embryos injected with Nanoblades programmed with two sgRNAs targeting the Tyr locus. Top-right panel, phenotype, editing efficiency (as measured by TIDE analysis of the Sanger-sequencing electropherograms) and the main INDEL type as detected by Sanger sequencing of individual PCR clones. Bottom-panel, alignment of individual PCR clones obtained from the Tyr locus of F0 mice against the mouse mm10 genome indicating the main observed INDELs in chimeric mice (mouse #4, #7, and #8) and total excision of the Tyr sequence between the sgRNA1 and sgRNA2 targeting loci for the complete albino mouse (mouse #3). The Sanger sequencing electropherogram from the bulk PCR amplicon obtained from mouse #3 indicates complete editing at both targeted sitesCC BY · PubMed Central

Fig. 5 Inactivation of Hpd in the liver of tyrosinaemic FRG mice. a Scheme of the experimental approach to target the liver of FRG mice. b T7 endonuclease assay to monitor genome editing at the Hpd gene in immortalized mouse macrophages and in the liver or spleen of injected mice. Samples were quantified using a Tapestation chipCC BY · PubMed Central

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome.
Langmead, B., Trapnell, C., Pop, M., & Salzberg, S. L. (2009) · Genome Biology · doi:10.1186/gb-2009-10-3-r25 · Cited by 21,849
HTSeq—a Python framework to work with high-throughput sequencing data
Anders, S., Pyl, P. T., & Huber, W. (2015) · Bioinformatics · doi:10.1093/bioinformatics/btu638 · Cited by 21,464
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions.
Kim, D., Pertea, G., Trapnell, C., Pimentel, H., Kelley, R., & Salzberg, S. L. (2013) · Genome Biology · doi:10.1186/gb-2013-14-4-r36 · Cited by 12,668
Development of Virus-Like Particle Technology from Small Highly Symmetric to Large Complex Virus-Like Particle Structures
Pushko, P., Pumpens, P., & Grens, E. (2013) · Intervirology · doi:10.1159/000346773 · Cited by 5,975
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761
Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017) · Nature · doi:10.1038/nature24644 · Cited by 4,184
Organoid Models and Applications in Biomedical Research
Xinaris, C., Brizi, V., & Remuzzi, G. (2015) · The Nephron journals/Nephron journals · doi:10.1159/000433566 · Cited by 3,519
High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells
Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon, D., Joung, J. K., & Sander, J. D. (2013) · Nature Biotechnology · doi:10.1038/nbt.2623 · Cited by 3,292
Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
Srinivas, S., Watanabe, T., Lin, C.-S., William, C. M., Tanabe, Y., Jessell, T. M., & Costantini, F. (2001) · BMC Developmental Biology · doi:10.1186/1471-213x-1-4 · Cited by 3,121
Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
Feng, G., Mellor, R. H., Bernstein, M., Keller-Peck, C., Nguyen, Q. T., Wallace, M., Nerbonne, J. M., Lichtman, J. W., & Sanes, J. R. (2000) · Neuron · doi:10.1016/s0896-6273(00)00084-2 · Cited by 3,042
‘Green mice’ as a source of ubiquitous green cells
Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T., & Nishimune, Y. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)00313-x · Cited by 2,581
Easy quantitative assessment of genome editing by sequence trace decomposition
Brinkman, E. K., Chen, T., Amendola, M., & van Steensel, B. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku936 · Cited by 2,390
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
Tsai, S. Q., Zheng, Z., Nguyen, N. T., Liebers, M., Topkar, V. V., Thapar, V., Wyvekens, N., Khayter, C., Iafrate, A. J., Le, L. P., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3117 · Cited by 2,268
Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System
Chen, B., Gilbert, L. A., Cimini, B. A., Schnitzbauer, J., Zhang, W., Li, G., Park, J. S., Blackburn, E. H., Weissman, J. S., Qi, L. S., et al. (2013) · Cell · doi:10.1016/j.cell.2013.12.001 · Cited by 1,988
Highly efficient Cas9-mediated transcriptional programming
Chavez, A., Scheiman, J., Vora, S., Pruitt, B. W., Tuttle, M., P R Iyer, E., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3312 · Cited by 1,622
Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo
Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. L., Hu, J. H., Maeder, M. L., Joung, J. K., Chen, Z.-Y., & Liu, D. R. (2015) · Nature Biotechnology · doi:10.1038/nbt.3081 · Cited by 1,460
-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin
Kinner, A., Wu, W., Staudt, C., & Iliakis, G. E. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn550 · Cited by 1,303
A Long Noncoding RNA Mediates Both Activation and Repression of Immune Response Genes
Carpenter, S. B., Aiello, D. A., Atianand, M., Ricci, E. P., Gandhi, P., Hall, L. L., Byron, M., Monks, B. G., Henry-Bezy, M., Lawrence, J. B., et al. (2013) · Science · doi:10.1126/science.1240925 · Cited by 957
Self-citation
Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo
Yin, H., Song, C.-Q., Dorkin, J. R., Zhu, L. J., Li, Y., Wu, Q., Park, A., Yang, J., Suresh, S., Bizhanova, A., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3471 · Cited by 876
Robust expansion of human hepatocytes in Fah−/−/Rag2−/−/Il2rg−/− mice
Azuma, H., Paulk, N., Ranade, A., Dorrell, C., Al-Dhalimy, M., Ellis, E., Strom, S., Kay, M. A., Finegold, M., & Grompe, M. (2007) · Nature Biotechnology · doi:10.1038/nbt1326 · Cited by 835
Assembly and release of HIV-1 precursor Pr55gag virus-like particles from recombinant baculovirus-infected insect cells
Gheysen, D., Jacobs, E. J., de Foresta, F., Thiriart, C., Francotte, M., Thines, D., & Wilde, M. D. (1989) · Cell · doi:10.1016/0092-8674(89)90873-8 · Cited by 743
Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing
Yin, H., Song, C.-Q., Suresh, S., Wu, Q., Walsh, S., Rhym, L. H., Mintzer, E., Bolukbasi, M. F., Zhu, L. J., Kauffman, K., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.4005 · Cited by 499
Safe harbours for the integration of new DNA in the human genome
Sadelain, M., Papapetrou, E. P., & Bushman, F. D. (2012) · Nature Reviews Cancer · doi:10.1038/nrc3179 · Cited by 441
CD81 Is Required for Hepatitis C Virus Glycoprotein-Mediated Viral Infection
Zhang, J., Randall, G. C., Higginbottom, A., Monk, P. N., Rice, C. M., & McKeating, J. A. (2004) · Journal of Virology · doi:10.1128/jvi.78.3.1448-1455.2004 · Cited by 361
Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes
Chen, S., Lee, B. J., Lee, A. Y.-F., Modzelewski, A. J., & He, L. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m116.733154 · Cited by 338
A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stagePreprint
van Sluis, M., & McStay, B. (2015) · Genes & Development · doi:10.1101/gad.260703.115 · Cited by 254
Therapeutic opportunities for manipulating TReg cells in autoimmunity and cancer
von Boehmer, H., & Daniel, C. (2012) · Nature Reviews Drug Discovery · doi:10.1038/nrd3683 · Cited by 230
Lentiviral vector retargeting to P-glycoprotein on metastatic melanoma through intravenous injection
Morizono, K., Xie, Y., Ringpis, G.-E. E., Johnson, M., Nassanian, H., Lee, B., Wu, L., & Chen, I. S. Y. (2005) · Nature Medicine · doi:10.1038/nm1192 · Cited by 220
Protein delivery using engineered virus-like particles
Kaczmarczyk, S. J., Sitaraman, K., Young, H. A., Hughes, S. H., & Chatterjee, D. K. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1101874108 · Cited by 220
Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor
Amirache, F., Lévy, C., Costa, C., Mangeot, P.-E., Torbett, B. E., Wang, C. X., Nègre, D., Cosset, F.-L., & Verhoeyen, E. (2014) · Blood · doi:10.1182/blood-2013-11-540641 · Cited by 197
Self-citation
Baboon envelope pseudotyped LVs outperform VSV-G-LVs for gene transfer into early-cytokine-stimulated and resting HSCs
Girard-Gagnepain, A., Amirache, F., Costa, C., Lévy, C., Frecha, C. A., Fusil, F., Négre, D., Lavillette, D., Cosset, F., & Verhoeyen, E. (2014) · Blood · doi:10.1182/blood-2014-02-558163 · Cited by 195
Self-citation
CRISPRseek: A Bioconductor Package to Identify Target-Specific Guide RNAs for CRISPR-Cas9 Genome-Editing Systems
Zhu, L. J., Holmes, B. R., D, N. A. M., & Brodsky, M. H. (2014) · PLoS ONE · doi:10.1371/journal.pone.0108424 · Cited by 192
Lentivirus pre-packed with Cas9 protein for safer gene editing
Choi, J., Dang, Y., Abraham, S., Ma, H., Zhang, J., Guo, H., Cai, Y., Mikkelsen, J. G., Wu, H., Shankar, P., et al. (2016) · Gene Therapy · doi:10.1038/gt.2016.27 · Cited by 192
Selective and nonselective packaging of cellular RNAs in retrovirus particles
Rulli, S. J., Hibbert, C. S., Mirro, J., Pederson, T., Biswal, S., & Rein, A. (2007) · Journal of Virology · doi:10.1128/jvi.02833-06 · Cited by 191
Marker-free coselection for CRISPR-driven genome editing in human cells
Agudelo, D., Duringer, A., Bozoyan, L., Huard, C., Carter, S., Loehr, J., Synodinou, D., Drouin, M., Salsman, J., Dellaire, G., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4265 · Cited by 183
Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering
Horii, T., Arai, Y., Yamazaki, M., Morita, S., Kimura, M., Itoh, M., Abe, Y., & Hatada, I. (2014) · Scientific Reports · doi:10.1038/srep04513 · Cited by 175
Ribosomal DNA copy number is coupled with gene expression variation and mitochondrial abundance in humans
Gibbons, J. G., Branco, A. T., Yu, S., & Lemos, B. (2014) · Nature Communications · doi:10.1038/ncomms5850 · Cited by 165
Stable transduction of quiescent T cells without induction of cycle progression by a novel lentiviral vector pseudotyped with measles virus glycoproteins
Frecha, C. A., Costa, C., Négre, D., Gauthier, E., Russell, S. J., Cosset, F., & Verhoeyen, E. (2008) · Blood · doi:10.1182/blood-2008-05-155945 · Cited by 165
Self-citation
In vivo genome editing in animals using AAV-CRISPR system: applications to translational research of human disease
Lau, C.-H., & Suh, Y. (2017) · F1000Research · doi:10.12688/f1000research.11243.1 · Cited by 161
Protein transduction from retroviral Gag precursors
Voelkel, C., Galla, M., Maetzig, T., Warlich, E., Kuehle, J., Zychlinski, D., Bode, J., Cantz, T., Schambach, A., & Baum, C. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914517107 · Cited by 138
Reprogramming metabolic pathways in vivo with CRISPR/Cas9 genome editing to treat hereditary tyrosinaemia
Pankowicz, F. P., Barzi, M., Legras, X. D., Hubert, L., Tian, M., Tomolonis, J., Ravishankar, M., Sun, Q., Yang, D., Borowiak, M., et al. (2016) · Nature Communications · doi:10.1038/ncomms12642 · Cited by 136
Selective Expression of the Cre Recombinase in Late-Stage Thymocytes Using the Distal Promoter of the Lck Gene
Zhang, D. J., Wang, Q., Wei, J., Baimukanova, G., Buchholz, F., Stewart, A. F., Mao, X., & Killeen, N. P. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.174.11.6725 · Cited by 132
Staufen1 senses overall transcript secondary structure to regulate translation
Ricci, E. P., Küçükural, A., Cenik, C., Mercier, B. C., Singh, G., Heyer, E. E., Ashar-Patel, A., Peng, L., & Moore, M. J. (2013) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2739 · Cited by 130
Self-citation
Protein Transfer Into Human Cells by VSV-G-induced Nanovesicles
Mangeot, P.-E., Dollet, S., Girard, M., Ciancia, C., Joly, S., Peschanski, M., & Lotteau, V. (2011) · Molecular Therapy · doi:10.1038/mt.2011.138 · Cited by 121
Self-citation
Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies
Wang, G., Yang, L., Grishin, D., Rios, X., Ye, L. Y., Hu, Y., Li, K., Zhang, D., Church, G. M., & Pu, W. T. (2016) · Nature Protocols · doi:10.1038/nprot.2016.152 · Cited by 117
Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
Yoon, Y., Wang, D., Tai, P. W. L., Riley, J., Gao, G., & Rivera‐Pérez, J. A. (2018) · Nature Communications · doi:10.1038/s41467-017-02706-7 · Cited by 94
Programmed Self-Assembly of an Active P22-Cas9 Nanocarrier System
Qazi, S., Miettinen, H. M., Wilkinson, R. A., McCoy, K., Douglas, T., & Wiedenheft, B. (2016) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.5b00822 · Cited by 87
Density Gradient Centrifugation of a Murine Leukemia Virus
O'Connor, T. E., Rauscher, F. J., & Zeigel, R. F. (1964) · Science · doi:10.1126/science.144.3622.1144 · Cited by 64
An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments
Heyer, E. E., Özadam, H., Ricci, E. P., Cenik, C., & Moore, M. J. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku1235 · Cited by 61
Self-citation
Redirecting Lentiviral Vectors Pseudotyped with Sindbis Virus-Derived Envelope Proteins to DC-SIGN by Modification of N-Linked Glycans of Envelope Proteins
Morizono, K., Ku, A. T., Xie, Y., Harui, A., Kung, S. K. P., Roth, M. D., Lee, B., & Chen, I. S. Y. (2010) · Journal of Virology · doi:10.1128/jvi.00435-10 · Cited by 57
Effects of two atypical neuroleptics, olanzapine and risperidone, on the function of the urinary bladder and the external urethral sphincter in anesthetized rats
Vera, P. L., Miranda-Sousa, A. J., & Nadelhaft, I. (2001) · BMC Pharmacology · doi:10.1186/1471-2210-1-4 · Cited by 38
Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells
Massouridès, E., Polentes, J., Mangeot, P.-E., Mournetas, V., Nectoux, J., Deburgrave, N., Nusbaum, P., Leturcq, F., Popplewell, L. J., Dickson, G., et al. (2015) · Skeletal Muscle · doi:10.1186/s13395-015-0062-6 · Cited by 37
Self-citation
Targeted retroviral vectors displaying a cleavage site-engineered hemagglutinin (HA) through HA–protease interactions
Szécsi, J., Drury, R., Josserand, V., Grange, M.-P., Boson, B., Hartl, I., Schneider, R. M., Buchholz, C. J., Coll, J., Russell, S. J., et al. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2006.04.007 · Cited by 33
Self-citation
VSV-G Envelope Glycoprotein Forms Complexes with Plasmid DNA and MLV Retrovirus-like Particles in Cell-free Conditions and Enhances DNA Transfection
Okimoto, T., Friedmann, T., & Miyanohara, A. (2001) · Molecular Therapy · doi:10.1006/mthe.2001.0443 · Cited by 30
Efficient Delivery and Nuclear Uptake Is Not Sufficient to Detect Gene Editing in CD34+ Cells Directed by a Ribonucleoprotein Complex
Modarai, S., Man, D., Bialk, P. A., Rivera-Torres, N., Bloh, K. M., & Kmiec, E. B. (2018) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2018.01.013 · Cited by 12

Sorted by citations

Exploring treatment options in cancer: tumor treatment strategies
Liu, B., Zhou, H., Tan, L., Siu, K. T. H., & Guan, X.-Y. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01856-7 · Cited by 1,201
CRISPR technology: A decade of genome editing is only the beginning
Wang, J. Y., & Doudna, J. A. (2023) · Science · doi:10.1126/science.add8643 · Cited by 1,105
Engineered Virus-like Particles for Efficient in Vivo Delivery of Therapeutic Proteins
Banskota, S., Raguram, A., Suh, S., Du, S. W., Davis, J. R., Choi, E. H., Wang, X., Nielsen, S. C., Newby, G. A., Randolph, P. B., et al. (2022) · Cell · doi:10.1016/j.cell.2021.12.021 · Cited by 668
Therapeutic in vivo delivery of gene editing agents
Raguram, A., Banskota, S., & Liu, D. R. (2022) · Cell · doi:10.1016/j.cell.2022.03.045 · Cited by 552
Autism genes converge on asynchronous development of shared neuron classes
Paulsen, B., Velasco, S., Kedaigle, A. J., Pigoni, M., Quadrato, G., Deo, A. J., Adiconis, X., Uzquiano, A., Sartore, R., Yang, S. M., et al. (2022) · Nature · doi:10.1038/s41586-021-04358-6 · Cited by 439

See all 346

Authors: Mangeot, P. E., Risson, V., Fusil, F., Marnef, A., Laurent, E., Blin, J., Mournetas, V., Massouridès, E., Sohier, T. J. M., Corbin, A., Aubé, F., Teixeira, M., Pinset, C., Schaeffer, L., Legube, G., Cosset, F.-L., Verhoeyen, E., Ohlmann, T., & Ricci, E. P.
Affiliations: Lyon 1 Université (FR)
Centre National de la Recherche Scientifique (FR)
Inserm (FR)
Corresponding author: Mangeot, Philippe E.
Ricci, Emiliano P.
ORCID: Risson, Valérie
Fusil, Floriane
Marnef, Aline
Laurent, Emilie
Blin, Juliana
Mournetas, Virginie
Massouridès, Emmanuelle
Sohier, Thibault J. M.
Corbin, Antoine
Aubé, Fabien
Pinset, Christian
Schaeffer, Laurent
Legube, Gaëlle
Cosset, François-Loïc
Verhoeyen, Els
Ohlmann, Théophile
Ricci, Emiliano P.
Venue: Nature Communications
Publisher: Springer Science and Business Media LLC
Published: 2019-01-03
Volume: 10
Issue: 1
Pages: 45-45
ISSN: 2041-1723 (ISSN-L)
DOI: 10.1038/s41467-018-07845-z
PMC: PMC6318322
OpenAlex: W2949383259
Cited by: 346
References: 56
Citations by year: 2026: 37 · 2025: 55 · 2024: 60 · 2023: 48 · 2022: 61 · 2021: 42 · 2020: 26 · 2019: 16
FWCI: 7.47
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0 (tdm)
creativecommons.org/licenses/by/4.0 (vor)
Locations: Nature Communications (publishedVersion, cc-by)
bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
Europe PMC (PubMed Central) (publishedVersion, other-oa)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
HAL (submittedVersion, other-oa)
HAL (submittedVersion)
RePEc: Research Papers in Economics (submittedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders: Styrelsen för Internationellt Utvecklingssamarbete
Fondation Innovations en Infectiologie
European Commission (HORIZON2020)
Agence Nationale de la Recherche (ANR-10-INBS-0009, ANR-11-IDEX-0007, ANR-11-LABX-0048, ANR-11-IDEX, ANR-10, IDEX-0007, 11 IDEX - 0007)
Institut National de la Santé et de la Recherche Médicale (ANR-11-IDEX-0007)
Centre National de la Recherche Scientifique
Université Claude Bernard Lyon 1 (ANR-11-IDEX-0007, ANR-11-LABX-0048)
Université de Lyon (ANR-11-IDEX-0 0 07, ANR-11-LABX-0048)
École Normale Supérieure de Lyon
Laboratoire d'Excellence Ecofect (ANR-11-IDEX-0007, ANR-11-LABX-0048)
H2020 European Research Council (805500)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords: genome editing, primary cells, in vivo gene editing, induced pluripotent stem cells, hematopoietic stem cells, bone marrow, homology-directed repair, transcriptional upregulation, mouse embryo
MeSH terms: Gene Editing, CRISPR-Associated Protein 9, Animals, DNA Repair, Embryo, Mammalian, Fibroblasts, Genetic Vectors, Hematopoietic Stem Cells, Humans, Macrophages, Mice, Inbred C57BL, Leukemia Virus, Murine, Ribonucleoproteins, Transcriptional Activation, Genome, Cell Line, Tumor, Mice, Induced Pluripotent Stem Cells, HEK293 Cells, Primary Cell Culture

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins

Abstract

Overview

Integrity signals

Figures

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks