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Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation
Open accessAbstract
Exosomes are attractive as nucleic-acid carriers because of their favourable pharmacokinetic and immunological properties and their ability to penetrate physiological barriers that are impermeable to synthetic drug-delivery vehicles. However, inserting exogenous nucleic acids, especially large messenger RNAs, into cell-secreted exosomes leads to low yields. Here we report a cellular-nanoporation method for the production of large quantities of exosomes containing therapeutic mRNAs and targeting peptides. We transfected various source cells with plasmid DNAs and stimulated the cells with a focal and transient electrical stimulus that promotes the release of exosomes carrying transcribed mRNAs and targeting peptides. Compared with bulk electroporation and other exosome-production strategies, cellular nanoporation produced up to 50-fold more exosomes and a more than 10 3-fold increase in exosomal mRNA transcripts, even from cells with low basal levels of exosome secretion. In orthotopic phosphatase and tensin homologue (PTEN)-deficient glioma mouse models, mRNA-containing exosomes restored tumour-suppressor function, enhanced inhibition of tumour growth and increased survival. Cellular nanoporation may enable the use of exosomes as a universal nucleic-acid carrier for applications requiring transcriptional manipulation.
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“Compared to exosomes produced endogenously without involving external stimuli, CNP-treated exosomes from cells of the same origin have an increased mRNA encapsulation efficiency of 3–4 folds.”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
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In the citing paper
“A tumour suppressor gene, like phosphatase and tensin homologue (PTEN), is commonly mutated in wide ranges of GBM, which may be a great candidate for therapeutic intervention27,54.”
From Blood–brain-barrier-crossing lipid nanoparticles for mRNA delivery to the central nervous system (2025) · cites this paper as [27]
Experimental — accuracy not yet measuredIn the citing paper
“The CNP technology increases the exosome yield and the mRNA amount within the exosomes [110].”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“In addition, this strategy increased mRNA loading in exosomes by 100-fold compared to conventional electroporation methods.”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“By modifying the exosome CD47 with these peptides, the targeting ability was improved.”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“Chemical modification of exosomes was carried out to target GL261 gliomas and U87 glioblastomas.”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“Moreover, when PTEN mRNA was additionally modified with the CDX peptide, the resulting exosomes exhibited a twofold increase in accumulation in mouse U87 gliomas, leading to prolonged survival.”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“Similarly, modification of PTEN mRNA with CREKA peptide resulted in a 1.5-fold increase in accumulation in mouse U87 gliomas and improved survival [110].”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“Yang et al. modified CD47-derived exosomes by incorporating a glioma targeting peptide at the N terminus. This modification allowed for selective uptake of tumor suppression gene, phosphatase and tensin homolog (PTEN) cargo by glioma cells. As a result, the proliferation of tumor cells was effectively inhibited [110].”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“In Yang et al. research, mouse embryonic fibroblast-derived exosomes were able to deliver PTEN (phosphatase and homologous tensin) mRNA to inhibit mouse glioma growth [49].”
From The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System (2022) · cites this paper as [49]
In the citing paper
“Naturally derived membrane lipids (for example, exosomes and cell membranes) can also be applied for mRNA delivery116,249,250.”
From Lipid nanoparticles for mRNA delivery (2021) · cites this paper as [249]
In the citing paper
“However, large-scale production and purification of natural exosomes present significant challenges. Standardization of isolation techniques remains an area of ongoing research, with variations in yield and purity across different methods [36].”
From Emerging role of exosomes in cancer therapy: progress and challenges (2025) · cites this paper as [36]
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Citations
Cites
Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes
Alvarez-Erviti, L., Seow, Y., Yin, H., Betts, C., Lakhal, S., & Wood, M. J. A. (2011) · Nature Biotechnology · doi:10.1038/nbt.1807 · Cited by 4,930
Extracellular vesicles: biology and emerging therapeutic opportunities
EL Andaloussi, S., Mäger, I., Breakefield, X. O., & Wood, M. J. A. (2013) · Nature Reviews Drug Discovery · doi:10.1038/nrd3978 · Cited by 3,571
Kowal, J., Arras, G., Colombo, M., Jouve, M., Morath, J. P., Primdal-Bengtson, B., Dingli, F., Loew, D., Tkach, M., & Théry, C. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1521230113 · Cited by 3,426
Lutz, M. B., Kukutsch, N. A., Ogilvie, A. L. J., Rößner, S., Koch, F., Romani, N., & Schuler, G. (1999) · Journal of Immunological Methods · doi:10.1016/s0022-1759(98)00204-x · Cited by 2,972
Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer
Kamerkar, S., LeBleu, V. S., Sugimoto, H., Yang, S., Ruivo, C. F., Melo, S. A., Lee, J. J., & Kalluri, R. (2017) · Nature · doi:10.1038/nature22341 · Cited by 2,639
Optimized exosome isolation protocol for cell culture supernatant and human plasma
Lobb, R. J., Becker, M., Wen, S., Wong, C. S. F., Wiegmans, A. P., Leimgruber, A., & Möller, A. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27031 · Cited by 1,763
Atheroprotective communication between endothelial cells and smooth muscle cells through miRNAs
Hergenreider, E., Heydt, S., Tréguer, K., Boettger, T., Horrevoets, A. J. G., Zeiher, A. M., Scheffer, M. P., Frangakis, A. S., Yin, X., Mayr, M., et al. (2012) · Nature Cell Biology · doi:10.1038/ncb2441 · Cited by 1,329
Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth
Zhang, L., Zhang, S., Yao, J., Lowery, F. J., Zhang, Q., Huang, W.-C., Li, P., Li, M., Wang, X., Zhang, C., et al. (2015) · Nature · doi:10.1038/nature15376 · Cited by 1,175
Self-citationSingle-molecule imaging of EGFR signalling on the surface of living cells
Sako, Y., Minoghchi, S., & Yanagida, T. (2000) · Nature Cell Biology · doi:10.1038/35004044 · Cited by 889
In vitro and ex vivo strategies for intracellular delivery
Stewart, M. P., Sharei, A., Ding, X., Sahay, G., Langer, R., & Jensen, K. F. (2016) · Nature · doi:10.1038/nature19764 · Cited by 885
Disruption of TET2 promotes the therapeutic efficacy of CD19-targeted T cells
Fraietta, J. A., Nobles, C. L., Sammons, M. A., Lundh, S., Carty, S. A., Reich, T. J., Cogdill, A. P., Morrissette, J. J. D., DeNizio, J. E., Reddy, S., et al. (2018) · Nature · doi:10.1038/s41586-018-0178-z · Cited by 862
Self-citationExosome Release Is Regulated by a Calcium-dependent Mechanism in K562 Cells
Savina, A., Furlán, M., Vidal, M., & Colombo, M. I. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m301642200 · Cited by 835
Generation and testing of clinical-grade exosomes for pancreatic cancer
Mendt, M. C., Kamerkar, S., Sugimoto, H., McAndrews, K. M., Wu, C.-C., Gagea, M., Yang, S., Blanko, E. V. R., Peng, Q., Ma, X., et al. (2018) · JCI Insight · doi:10.1172/jci.insight.99263 · Cited by 812
Kojima, R., Bojar, D., Rizzi, G., Hamri, G. C.-E., El-Baba, M. D., Saxena, P., Ausländer, S., Tan, K. R., & Fussenegger, M. (2018) · Nature Communications · doi:10.1038/s41467-018-03733-8 · Cited by 748
Mesenchymal stem cell: An efficient mass producer of exosomes for drug delivery
Yeo, R. W. Y., Lai, R. C., Zhang, B., Tan, S. S., Yin, Y., Teh, B. J., & Lim, S. K. (2013) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.07.001 · Cited by 746
The Regulation of Exosome Secretion: a Novel Function of the p53 Protein
Yu, X., Harris, S. L., & Levine, A. J. (2006) · Cancer Research · doi:10.1158/0008-5472.can-05-4579 · Cited by 688
Efficient RNA drug delivery using red blood cell extracellular vesicles
Usman, W. M., Pham, T. C., Kwok, Y. Y., Vu, L. T., Ma, V., Peng, B., Chan, Y. S., Wei, L., Chin, S. M., Azad, A., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-04791-8 · Cited by 663
Self-citationTemperature Measurement in Microfluidic Systems Using a Temperature-Dependent Fluorescent Dye
Ross, D., Gaitan, M., & Locascio, L. E. (2001) · Analytical Chemistry · doi:10.1021/ac010370l · Cited by 628
Biology and biogenesis of shed microvesicles
Tricarico, C. J., Clancy, J. W., & D’Souza‐Schorey, C. (2016) · Small GTPases · doi:10.1080/21541248.2016.1215283 · Cited by 618
Immune evasion mediated by PD-L1 on glioblastoma-derived extracellular vesicles
Ricklefs, F. L., Alayo, Q., Krenzlin, H., Mahmoud, A. B., Speranza, M. C., Nakashima, H., Hayes, J. L., Lee, K., Balaj, L., Passaro, C., et al. (2018) · Science Advances · doi:10.1126/sciadv.aar2766 · Cited by 610
Hypothalamic stem cells control ageing speed partly through exosomal miRNAs
Zhang, Y., Kim, M. S., Jia, B., Yan, J., Zuniga-Hertz, J. P., Han, C., & Cai, D. (2017) · Nature · doi:10.1038/nature23282 · Cited by 564
Coding and noncoding landscape of extracellular RNA released by human glioma stem cells
Wei, Z., Batagov, A. O., Schinelli, S., Wang, J., Wang, Y., Fatimy, R. E., Rabinovsky, R., Balaj, L., Chen, C. C., Hochberg, F. H., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-01196-x · Cited by 546
Self-citationLespagnol, A., Duflaut, D., Beekman, C., Blanc, L., Fiucci, G., Marine, J., Vidal, M., Amson, R. B., & Telerman, A. (2008) · Cell Death and Differentiation · doi:10.1038/cdd.2008.104 · Cited by 348
Nanochannel electroporation delivers precise amounts of biomolecules into living cells
Boukany, P. E., Morss, A., Liao, W., Henslee, B. E., Jung, H.-C., Zhang, X., Yu, B., Wang, X., Wu, Y., Li, L., et al. (2011) · Nature Nanotechnology · doi:10.1038/nnano.2011.164 · Cited by 340
Self-citationLam, F. C., Morton, S. W., Wyckoff, J. B., Vu‐Han, T., Hwang, M. K., Maffa, A., Balkanska-Sinclair, E., Yaffe, M. B., Floyd, S., & Hammond, P. T. (2018) · Nature Communications · doi:10.1038/s41467-018-04315-4 · Cited by 334
Ad26/MVA therapeutic vaccination with TLR7 stimulation in SIV-infected rhesus monkeys
Borducchi, E. N., Cabral, C., Stephenson, K. E., Liu, J., Abbink, P., Ng’ang’a, D., Nkolola, J. P., Brinkman, A. L., Peter, L. E., Lee, B. C., et al. (2016) · Nature · doi:10.1038/nature20583 · Cited by 279
ARMMs as a versatile platform for intracellular delivery of macromolecules
Wang, Q., Yu, J., Kadungure, T., Beyene, J., Zhang, H., & Lu, Q. (2018) · Nature Communications · doi:10.1038/s41467-018-03390-x · Cited by 275
Sun, T., Zhang, Y., Power, C., Alexander, P. M., Sutton, J. T., Aryal, M., Vykhodtseva, N. I., Miller, E. L., & McDannold, N. J. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1713328114 · Cited by 255
A Ca2+-stimulated exosome release pathway in cancer cells is regulated by Munc13-4
Messenger, S. W., Woo, S. S., Sun, Z., & Martin, T. F. J. (2018) · The Journal of Cell Biology · doi:10.1083/jcb.201710132 · Cited by 236
Functional exosome-mimic for delivery of siRNA to cancer: in vitro and in vivo evaluation
Yang, Z., Xie, J., Zhu, J., Chen, K., Chiang, C., Wang, X., Wang, X., Kuang, T., Chen, F., Chen, Z., et al. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.10.008 · Cited by 219
Self-citationRadiation-Induced Targeted Nanoparticle-Based Gene Delivery for Brain Tumor Therapy
Erel‐Akbaba, G., de Carvalho, L. A., Tian, T., Zinter, M., Akbaba, H., Obeid, P. J., Chiocca, E. A., Weissleder, R., Kantarcı, A. G., & Tannous, B. A. (2019) · ACS Nano · doi:10.1021/acsnano.8b08177 · Cited by 208
A D ‐Peptide Ligand of Nicotine Acetylcholine Receptors for Brain‐Targeted Drug Delivery
Wei, X., Zhan, C., Shen, Q., Fu, W., Xie, C., Gao, J., Peng, C., Zheng, P., & Lu, W. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201411226 · Cited by 181
Self-citationKao, C., & Papoutsakis, E. T. (2019) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2019.01.005 · Cited by 175
Microscopic structure of the polymer-induced liquid precursor for calcium carbonate
Xu, Y., Tijssen, K. C. H., Bomans, P. H. H., Akiva, A., Friedrich, H., Kentgens, A. P. M., & Sommerdijk, N. A. J. M. (2018) · Nature Communications · doi:10.1038/s41467-018-05006-w · Cited by 171
Topical tissue nano-transfection mediates non-viral stroma reprogramming and rescue
Gallego‐Perez, D., Pal, D., Ghatak, S., Malkoc, V., Higuita‐Castro, N., Gnyawali, S. C., Chang, L., Liao, W., Shi, J., Sinha, M., et al. (2017) · Nature Nanotechnology · doi:10.1038/nnano.2017.134 · Cited by 165
Self-citationFibrin-binding, peptide amphiphile micelles for targeting glioblastoma
Chung, E. J., Cheng, Y., Morshed, R. A., Nord, K., Han, Y., Wegscheid, M. L., Auffinger, B., Wainwright, D. A., Lesniak, M. S., & Tirrell, M. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.10.064 · Cited by 162
Hsp90 Regulates the Activity of Wild Type p53 under Physiological and Elevated Temperatures
Müller, L., Schaupp, A., Walerych, D., Wegele, H., & Büchner, J. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m407687200 · Cited by 156
Khushman, M. M., Bhardwaj, A., Patel, G. K., Laurini, J. A., Roveda, K. P., Tan, M. C. B., Patton, M. C., Singh, S., Taylor, W. R., & Singh, A. P. (2017) · Pancreas · doi:10.1097/mpa.0000000000000847 · Cited by 110
Walerych, D., Olszewski, M. B., Gutkowska, M., Helwak, A., Żylicz, M., & Żylicz, M. (2009) · Oncogene · doi:10.1038/onc.2009.281 · Cited by 82
EVIR: chimeric receptors that enhance dendritic cell cross-dressing with tumor antigens
Squadrito, M. L., Cianciaruso, C., Hansen, S. K., & Palma, M. D. (2018) · Nature Methods · doi:10.1038/nmeth.4579 · Cited by 79
Wang, W., Li, J., Wu, K., Azhati, B., & Rexiati, M. (2016) · Medical Science Monitor · doi:10.12659/msm.896951 · Cited by 74
Singh, A. K., Kim, W., Kim, Y., Jeong, K., Kang, C. S., Kim, Y. S., Koh, J., Mahajan, S. D., Prasad, P. N., & Kim, S. (2016) · Advanced Functional Materials · doi:10.1002/adfm.201602808 · Cited by 70
Self-citationMane, D. R., Kale, A. D., & Belaldavar, C. (2017) · Journal of Oral and Maxillofacial Pathology · doi:10.4103/jomfp.jomfp_234_16 · Cited by 61
Lee, L. J., Yang, Z., Rahman, M. A., Ma, J., Kwak, K. J., McElroy, J., Shilo, K., Goparaju, C. M. V., Yu, L., Rom, W. N., et al. (2016) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201511-2129le · Cited by 50
Self-citationGallego‐Perez, D., Otero, J. J., Czeisler, C. M., Ma, J., Ortiz, C., Gygli, P. E., Catacutan, F. P., Gokozan, H. N., Cowgill, A., Sherwood, T. W., et al. (2015) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2015.11.015 · Cited by 48
Self-citationProtective effect of α-mangostin against iodixanol-induced apoptotic damage in LLC-PK1 cells
Lee, D., Choi, Y. O., Kim, K. H., Chin, Y., Namgung, H., Yamabe, N., & Jung, K. (2016) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2016.05.031 · Cited by 14
UK scientists test liposome gene therapy technique
Dickson, D. (1993) · Nature · doi:10.1038/365004a0 · Cited by 11
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Metadata
- Authors
- Yang, Z., Shi, J., Xie, J., Wang, Y., Sun, J., Liu, T., Zhao, Y., Zhao, X., Wang, X., Ma, Y., Malkoc, V., Chiang, C., Deng, W., Chen, Y., Fu, Y., Kwak, K. J., Fan, Y., Kang, C., Yin, C., Rhee, J., Bertani, P., Otero, J., Lu, W., Yun, K., Lee, A. S., Jiang, W., Teng, L., Kim, B. Y. S., & Lee, L. J.
- Affiliations
- The Ohio State University (US)
- Jilin University (CN)
- The University of Texas Southwestern Medical Center (US)
- Jinan University (CN)
- Mayo Clinic (US)
- Peking University (CN)
- Institute for Stem Cell Biology and Regenerative Medicine (US)
- Houston Methodist Research Institute (US)
- Shenzhen Bay Laboratory (CN)
- Corresponding author
- Teng, Lesheng
- Kim, Betty Y. S.
- Lee, L. James
- ORCID
- Venue
- Nature Biomedical Engineering
- Published
- 2019-12-16
- Volume
- 4
- Issue
- 1
- Pages
- 69-83
- ISSN
- 2157-846X (ISSN-L)
- PMC
- PMC7080209
- OpenAlex
- W2995128645
- Cited by
- 744
- References
- 49
- Citations by year
- 2026: 81 · 2025: 125 · 2024: 135 · 2023: 128 · 2022: 117 · 2021: 101 · 2020: 56
- FWCI
- 22.67
- Type
- journal article
- Language
- en
- Update notices
- correction (2021-06-15)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Nature Biomedical Engineering (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Department of Health & Human Services | NIH | National Cancer Institute (K08CA241070)
- U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (1R01NS104315)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- exosomes, mRNA delivery, electroporation, exosome production, targeting peptides, tumor suppression, nucleic acid carriers, exosome secretion, brain tumors
- MeSH terms
- Animals, Brain Neoplasms, Cells, Cultured, Disease Models, Animal, Glioma, Humans, Mice, Inbred C57BL, RNA, Messenger, Signal Transduction, Drug Delivery Systems, Electroporation, MicroRNAs, Nanotechnology, Exosomes, HEK293 Cells
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