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Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation
Open accessAbstract
Exosomes are attractive as nucleic-acid carriers because of their favourable pharmacokinetic and immunological properties and their ability to penetrate physiological barriers that are impermeable to synthetic drug-delivery vehicles. However, inserting exogenous nucleic acids, especially large messenger RNAs, into cell-secreted exosomes leads to low yields. Here we report a cellular-nanoporation method for the production of large quantities of exosomes containing therapeutic mRNAs and targeting peptides. We transfected various source cells with plasmid DNAs and stimulated the cells with a focal and transient electrical stimulus that promotes the release of exosomes carrying transcribed mRNAs and targeting peptides. Compared with bulk electroporation and other exosome-production strategies, cellular nanoporation produced up to 50-fold more exosomes and a more than 10 3-fold increase in exosomal mRNA transcripts, even from cells with low basal levels of exosome secretion. In orthotopic phosphatase and tensin homologue (PTEN)-deficient glioma mouse models, mRNA-containing exosomes restored tumour-suppressor function, enhanced inhibition of tumour growth and increased survival. Cellular nanoporation may enable the use of exosomes as a universal nucleic-acid carrier for applications requiring transcriptional manipulation.
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“Compared to exosomes produced endogenously without involving external stimuli, CNP-treated exosomes from cells of the same origin have an increased mRNA encapsulation efficiency of 3–4 folds.”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
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In the citing paper
“A tumour suppressor gene, like phosphatase and tensin homologue (PTEN), is commonly mutated in wide ranges of GBM, which may be a great candidate for therapeutic intervention27,54.”
From Blood–brain-barrier-crossing lipid nanoparticles for mRNA delivery to the central nervous system (2025) · cites this paper as [27]
Experimental — accuracy not yet measuredIn the citing paper
“The CNP technology increases the exosome yield and the mRNA amount within the exosomes [110].”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“In addition, this strategy increased mRNA loading in exosomes by 100-fold compared to conventional electroporation methods.”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“By modifying the exosome CD47 with these peptides, the targeting ability was improved.”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“Chemical modification of exosomes was carried out to target GL261 gliomas and U87 glioblastomas.”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“Moreover, when PTEN mRNA was additionally modified with the CDX peptide, the resulting exosomes exhibited a twofold increase in accumulation in mouse U87 gliomas, leading to prolonged survival.”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“Similarly, modification of PTEN mRNA with CREKA peptide resulted in a 1.5-fold increase in accumulation in mouse U87 gliomas and improved survival [110].”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“Yang et al. modified CD47-derived exosomes by incorporating a glioma targeting peptide at the N terminus. This modification allowed for selective uptake of tumor suppression gene, phosphatase and tensin homolog (PTEN) cargo by glioma cells. As a result, the proliferation of tumor cells was effectively inhibited [110].”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [110]
In the citing paper
“In Yang et al. research, mouse embryonic fibroblast-derived exosomes were able to deliver PTEN (phosphatase and homologous tensin) mRNA to inhibit mouse glioma growth [49].”
From The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System (2022) · cites this paper as [49]
In the citing paper
“Naturally derived membrane lipids (for example, exosomes and cell membranes) can also be applied for mRNA delivery116,249,250.”
From Lipid nanoparticles for mRNA delivery (2021) · cites this paper as [249]
In the citing paper
“However, large-scale production and purification of natural exosomes present significant challenges. Standardization of isolation techniques remains an area of ongoing research, with variations in yield and purity across different methods [36].”
From Emerging role of exosomes in cancer therapy: progress and challenges (2025) · cites this paper as [36]
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Citations
Cites
Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes
Alvarez-Erviti, L., Seow, Y., Yin, H., Betts, C., Lakhal, S., & Wood, M. J. A. (2011) · Nature Biotechnology · doi:10.1038/nbt.1807 · Cited by 4,930
Extracellular vesicles: biology and emerging therapeutic opportunities
EL Andaloussi, S., Mäger, I., Breakefield, X. O., & Wood, M. J. A. (2013) · Nature Reviews Drug Discovery · doi:10.1038/nrd3978 · Cited by 3,571
Kowal, J., Arras, G., Colombo, M., Jouve, M., Morath, J. P., Primdal-Bengtson, B., Dingli, F., Loew, D., Tkach, M., & Théry, C. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1521230113 · Cited by 3,426
Lutz, M. B., Kukutsch, N. A., Ogilvie, A. L. J., Rößner, S., Koch, F., Romani, N., & Schuler, G. (1999) · Journal of Immunological Methods · doi:10.1016/s0022-1759(98)00204-x · Cited by 2,972
Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer
Kamerkar, S., LeBleu, V. S., Sugimoto, H., Yang, S., Ruivo, C. F., Melo, S. A., Lee, J. J., & Kalluri, R. (2017) · Nature · doi:10.1038/nature22341 · Cited by 2,639
Optimized exosome isolation protocol for cell culture supernatant and human plasma
Lobb, R. J., Becker, M., Wen, S., Wong, C. S. F., Wiegmans, A. P., Leimgruber, A., & Möller, A. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27031 · Cited by 1,763
Atheroprotective communication between endothelial cells and smooth muscle cells through miRNAs
Hergenreider, E., Heydt, S., Tréguer, K., Boettger, T., Horrevoets, A. J. G., Zeiher, A. M., Scheffer, M. P., Frangakis, A. S., Yin, X., Mayr, M., et al. (2012) · Nature Cell Biology · doi:10.1038/ncb2441 · Cited by 1,329
Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth
Zhang, L., Zhang, S., Yao, J., Lowery, F. J., Zhang, Q., Huang, W.-C., Li, P., Li, M., Wang, X., Zhang, C., et al. (2015) · Nature · doi:10.1038/nature15376 · Cited by 1,175
Self-citation
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Hou, X., Zaks, T., Langer, R., & Dong, Y. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00358-0 · Cited by 4,076
Extracellular vesicles as a next-generation drug delivery platform
Herrmann, I. K., Wood, M. J. A., & Fuhrmann, G. (2021) · Nature Nanotechnology · doi:10.1038/s41565-021-00931-2 · Cited by 1,948
Lipids and Lipid Derivatives for RNA Delivery
Zhang, Y., Sun, C., Wang, C., Jankovic, K. E., & Dong, Y. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00244 · Cited by 649
Exosomes─Nature’s Lipid Nanoparticles, a Rising Star in Drug Delivery and Diagnostics
Tenchov, R., Sasso, J. M., Wang, X., Liaw, W., Chen, C., & Zhou, Q. A. (2022) · ACS Nano · doi:10.1021/acsnano.2c08774 · Cited by 642
Self-citationDesign of therapeutic biomaterials to control inflammation
Tu, Z., Zhong, Y., Hu, H., Shao, D., Haag, R., Schirner, M., Lee, J., Sullenger, B. A., & Leong, K. W. (2022) · Nature Reviews Materials · doi:10.1038/s41578-022-00426-z · Cited by 533
The landscape of mRNA nanomedicine
Huang, X., Kong, N., Zhang, X., Cao, Y., Langer, R., & Tao, W. (2022) · Nature Medicine · doi:10.1038/s41591-022-02061-1 · Cited by 511
mRNA-based cancer therapeutics
Liu, C., Shi, Q., Huang, X., Koo, S., Kong, N., & Tao, W. (2023) · Nature Reviews Cancer · doi:10.1038/s41568-023-00586-2 · Cited by 500
Lipid Nanoparticle (LNP) Enables mRNA Delivery for Cancer Therapy
Zong, Y., Lin, Y., Wei, T., & Cheng, Q. (2023) · Advanced Materials · doi:10.1002/adma.202303261 · Cited by 506
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Kim, H. I., Park, J., Zhu, Y., Wang, X., Han, Y., & Zhang, D. (2024) · Experimental & Molecular Medicine · doi:10.1038/s12276-024-01201-6 · Cited by 505
Self-citationXie, J., Bi, Y., Zhang, H., Dong, S., Teng, L., Lee, R. J., & Yang, Z. (2020) · Frontiers in Pharmacology · doi:10.3389/fphar.2020.00697 · Cited by 497
Self-citationExosome engineering: Current progress in cargo loading and targeted delivery
Fu, S., Wang, Y., Xia, X., & Zheng, J. C. (2020) · NanoImpact · doi:10.1016/j.impact.2020.100261 · Cited by 444
Self-citationCurrent Strategies for Exosome Cargo Loading and Targeting Delivery
Zeng, H., Guo, S., Ren, X., Wu, Z., Liu, S., & Yao, X. (2023) · Cells · doi:10.3390/cells12101416 · Cited by 464
Gupta, D., Zickler, A. M., & Andaloussi, S. E. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.113961 · Cited by 438
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Zhang, X., Zhang, H., Gu, J., Zhang, J., Shi, H., Qian, H., Wang, D., Xu, W., Pan, J., & Santos, H. A. (2021) · Advanced Materials · doi:10.1002/adma.202005709 · Cited by 428
The challenge and prospect of mRNA therapeutics landscape
Weng, Y., Li, C., Yang, T., Hu, B., Zhang, M., Guo, S., Xiao, H., Liang, X.-J., & Huang, Y. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107534 · Cited by 417
Engineered exosomes as an in situ DC-primed vaccine to boost antitumor immunity in breast cancer
Huang, L., Rong, Y., Tang, X., Yi, K., Qi, P., Hou, J., Liu, W., He, Y. Z., Gao, X., Yuan, C., et al. (2022) · Molecular Cancer · doi:10.1186/s12943-022-01515-x · Cited by 416
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Duan, L., Ouyang, K., Xu, X., Xu, L., Wen, C., Zhou, X., Qin, Z., Xu, Z., Sun, W., & Liang, Y. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.673286 · Cited by 377
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Teng, F., & Fussenegger, M. (2020) · Advanced Science · doi:10.1002/advs.202003505 · Cited by 401
Recent progress in targeted delivery vectors based on biomimetic nanoparticles
Chen, L., Hong, W., Ren, W., Xu, T., Qian, Z., & He, Z. (2021) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-021-00631-2 · Cited by 401
Extracellular vesicles: A bright star of nanomedicine
Wu, P., Zhang, B., Ocansey, D. K. W., Xu, W., & Qian, H. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2020.120467 · Cited by 368
Wu, T., Liu, Y., Cao, Y., & Liu, Z. (2022) · Advanced Materials · doi:10.1002/adma.202110364 · Cited by 357
Non-viral vectors for RNA delivery
Yan, Y., Liu, X.-Y., Lu, A., Wang, X.-Y., Jiang, L.-X., & Wang, J.-C. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.01.008 · Cited by 332
Self-citationRecent advances in exosome-mediated nucleic acid delivery for cancer therapy
Zhang, Y., Liu, Q., Zhang, X., Huang, H., Tang, S., Chai, Y., Xu, Z., Li, M., Chen, X., Liu, J., et al. (2022) · Journal of Nanobiotechnology · doi:10.1186/s12951-022-01472-z · Cited by 306
Shao, J., Zaro, J., & Shen, Y. (2020) · International Journal of Nanomedicine · doi:10.2147/ijn.s281890 · Cited by 295
Emerging role of exosomes in cancer therapy: progress and challenges
Li, J., Wang, J., & Chen, Z. (2025) · Molecular Cancer · doi:10.1186/s12943-024-02215-4 · Cited by 297
Exploiting the biogenesis of extracellular vesicles for bioengineering and therapeutic cargo loading
Rädler, J., Gupta, D., Zickler, A. M., & Andaloussi, S. E. (2023) · Molecular Therapy · doi:10.1016/j.ymthe.2023.02.013 · Cited by 298
Biodistribution of Exosomes and Engineering Strategies for Targeted Delivery of Therapeutic Exosomes
Choi, H., Choi, Y., Yim, H. Y., Mirzaaghasi, A., Yoo, J., & Choi, C. (2021) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-021-00361-0 · Cited by 295
Emerging Prospects of Exosomes for Cancer Treatment: From Conventional Therapy to Immunotherapy
Nam, G., Choi, Y., Kim, G. B., Kim, S., Kim, S. A., & Kim, I. (2020) · Advanced Materials · doi:10.1002/adma.202002440 · Cited by 270
Exosome-Based Drug Delivery: Translation from Bench to Clinic
Koh, H. B., Kim, H. J., Kang, S.-W., & Yoo, T.-H. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15082042 · Cited by 259
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You, Y., Tian, Y., Yang, Z., Shi, J., Kwak, K. J., Tong, Y., Estania, A. P., Cao, J., Hsu, W.-H., Liu, Y., et al. (2023) · Nature Biomedical Engineering · doi:10.1038/s41551-022-00989-w · Cited by 258
Self-citationHuda, M. N., Nafiujjaman, M., Deaguero, I. G., Okonkwo, J., Hill, M. L., Kim, T. H., & Nurunnabi, M. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c00217 · Cited by 268
Exosome-mediated delivery of gene vectors for gene therapy
Duan, L., Xu, L., Xu, X., Qin, Z., Zhou, X., Xiao, Y., Liang, Y., & Xia, J. (2021) · Nanoscale · doi:10.1039/d0nr07622h · Cited by 238
Smart drug delivery systems for precise cancer therapy
Wang, X., Li, C., Wang, Y., Chen, H., Zhang, X., Luo, C., Zhou, W., Li, L., Teng, L., Yu, H., et al. (2022) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2022.08.013 · Cited by 256
Self-citationCamouflage strategies for therapeutic exosomes evasion from phagocytosis
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Cheng, K., & Kalluri, R. (2023) · Extracellular Vesicle · doi:10.1016/j.vesic.2023.100029 · Cited by 231
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Zhao, X., Wu, D., Ma, X., Wang, J., Hou, W., & Zhang, W. (2020) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2020.110237 · Cited by 197
Self-citationExtracellular vesicles as a drug delivery system: A systematic review of preclinical studies
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Self-citationDevelopment of Novel Therapeutics Targeting the Blood–Brain Barrier: From Barrier to Carrier
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Yuan, F., Li, Y., & Wang, Z. (2021) · Drug Delivery · doi:10.1080/10717544.2021.1951896 · Cited by 199
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Zhong, J., Xia, B., Shan, S., Zheng, A., Zhang, S., Chen, J., & Liang, X. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.121126 · Cited by 180
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Yan, L., Shen, J., Wang, J., Yang, X., Dong, S., & Lu, S. (2020) · Dose-Response · doi:10.1177/1559325820936161 · Cited by 182
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Self-citationAdvances in Multiple Stimuli-Responsive Drug-Delivery Systems for Cancer Therapy
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Self-citationRole of exosomes in the pathogenesis, diagnosis, and treatment of central nervous system diseases
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Self-citationExtracellular vesicle-based Nanotherapeutics: Emerging frontiers in anti-inflammatory therapy
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Zhang, W., Jiang, Y., He, Y., Boucetta, H., Wu, J., Chen, Z., & He, W. (2022) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2022.11.026 · Cited by 134
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Tang, T., Wang, B., Li, Z., Wen, Y., Feng, S., Wu, M., Liu, D., Cao, J.-Y., Yin, Q., Yin, D., et al. (2021) · Journal of the American Society of Nephrology · doi:10.1681/asn.2020111561 · Cited by 129
Self-citationAmbattu, L. A., Ramesan, S., Dekiwadia, C. D., Hanssen, E. G., Li, H., & Yeo, L. Y. (2020) · Communications Biology · doi:10.1038/s42003-020-01277-6 · Cited by 123
Bu, T., Li, Z., Hou, Y., Sun, W., Zhang, R., Zhao, L., Wei, M., Yang, G., & Yuan, L. (2021) · Theranostics · doi:10.7150/thno.64229 · Cited by 134
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Self-citationGhoroghi, S., Mary, B., Larnicol, A., Asokan, N., Klein, A., Osmani, N., Busnelli, I., Delalande, F., Paul, N., Halary, S., et al. (2021) · eLife · doi:10.7554/elife.61539 · Cited by 127
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Exosomes as smart drug delivery vehicles for cancer immunotherapy
Zhang, H., Wang, S., Sun, M., Cui, Y., Xing, J., Teng, L., Xi, Z., & Yang, Z. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2022.1093607 · Cited by 119
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Zou, Z., Han, L., Xu, G., Hu, Y., Zhang, W., & Tian, K. (2023) · International Journal of Nanomedicine · doi:10.2147/ijn.s417422 · Cited by 115
The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System
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Li, T., Li, J., Chen, Z., Zhang, S., Li, S., Wageh, S., Al‐Hartomy, O. A., Al-Sehemi, A. G., Xie, Z., Kankala, R. K., et al. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.09.065 · Cited by 113
Strategies to overcome the main challenges of the use of exosomes as drug carrier for cancer therapy
Hussen, B. M., Faraj, G. S. H., Rasul, M. F., Hidayat, H. J., Salihi, A. B., Baniahmad, A., Taheri, M. N., & Ghafouri‐Fard, S. (2022) · Cancer Cell International · doi:10.1186/s12935-022-02743-3 · Cited by 113
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Aslan, C., Kiaie, S. H., Zolbanin, N. M., Lotfinejad, P., Ramezani, R., Kashanchi, F., & Jafari, R. (2021) · BMC Biotechnology · doi:10.1186/s12896-021-00683-w · Cited by 113
Exosome-based Ldlr gene therapy for familial hypercholesterolemia in a mouse model
Li, Z., Zhao, P., Zhang, Y., Wang, J., Wang, C., Liu, Y., Yang, G., & Yuan, L. (2021) · Theranostics · doi:10.7150/thno.49874 · Cited by 108
Targeted delivery of extracellular vesicles in heart injury
Chen, P., Wang, L., Fan, X., Ning, X., Yu, B., Ou, C., & Chen, M. (2020) · Theranostics · doi:10.7150/thno.51571 · Cited by 107
Advances in mRNA non-viral delivery approaches
Ibba, M. L., Ciccone, G., Esposito, C. L., Catuogno, S., & Giangrande, P. H. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.113930 · Cited by 107
Recent Advances in Microscale Electroporation
Choi, S., Khoo, H., & Hur, S. C. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00677 · Cited by 109
Li, F., Wu, J., Li, D., Hao, L., Li, Y., Yi, D., Yeung, K. W. K., Chen, D., Lu, W. W., Pan, H., et al. (2022) · Journal of Nanobiotechnology · doi:10.1186/s12951-022-01347-3 · Cited by 99
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Lü, Z., Shen, J., Yang, H. J., Wang, J., Zhao, R., Zhang, T.-L., Guo, J. R., & Zhang, X. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01298-z · Cited by 116
Xia, B., Gao, J., Li, S., Huang, L., Zhu, L., Ma, T., Zhao, L., Yang, Y., Luo, K., Xiaowei, et al. (2020) · Theranostics · doi:10.7150/thno.44912 · Cited by 100
Jakubik, D., Fitas, A., Eyileten, C., Jarosz-Popek, J., Nowak, A., Czajka, P., Wicik, Z., Sourij, H., Siller-Matula, J. M., Rosa, S. D., et al. (2021) · Cardiovascular Diabetology · doi:10.1186/s12933-021-01245-2 · Cited by 100
Engineered extracellular vesicles as intelligent nanosystems for next-generation nanomedicine
Fan, Z., Jiang, C., Wang, Y., Wang, K., Marsh, J., Zhang, D., Chen, X., & Nie, L. (2022) · Nanoscale Horizons · doi:10.1039/d2nh00070a · Cited by 103
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Khatami, S. H., Karami, N., Taheri‐Anganeh, M., Taghvimi, S., Tondro, G., Khorsand, M., Fard, E. S., Sedighimehr, N., Kazemi, M., Jaberi, K. R., et al. (2023) · Molecular Neurobiology · doi:10.1007/s12035-023-03365-0 · Cited by 109
Yang, G., Waheed, S., Wang, C., Shekh, M. I., Li, Z., & Wu, J. (2023) · International Journal of Biological Sciences · doi:10.7150/ijbs.80430 · Cited by 106
Exosomal mRNAs for Angiogenic–Osteogenic Coupled Bone Repair
Ma, Y., Sun, L., Zhang, J., Chiang, C., Pan, J., Wang, X., Kwak, K. J., Li, H., Zhao, R., Rima, X. Y., et al. (2023) · Advanced Science · doi:10.1002/advs.202302622 · Cited by 103
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Bader, J., Brigger, F., & Leroux, J. (2024) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2024.115461 · Cited by 110
Exosomal PD‐L1: New Insights Into Tumor Immune Escape Mechanisms and Therapeutic Strategies
Zhou, K., Guo, S., Li, F., Sun, Q., & Liang, G. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.569219 · Cited by 99
Metadata
- Authors
- Yang, Z., Shi, J., Xie, J., Wang, Y., Sun, J., Liu, T., Zhao, Y., Zhao, X., Wang, X., Ma, Y., Malkoc, V., Chiang, C., Deng, W., Chen, Y., Fu, Y., Kwak, K. J., Fan, Y., Kang, C., Yin, C., Rhee, J., Bertani, P., Otero, J., Lu, W., Yun, K., Lee, A. S., Jiang, W., Teng, L., Kim, B. Y. S., & Lee, L. J.
- Affiliations
- The Ohio State University (US)
- Jilin University (CN)
- The University of Texas Southwestern Medical Center (US)
- Jinan University (CN)
- Mayo Clinic (US)
- Peking University (CN)
- Institute for Stem Cell Biology and Regenerative Medicine (US)
- Houston Methodist Research Institute (US)
- Shenzhen Bay Laboratory (CN)
- Corresponding author
- Teng, Lesheng
- Kim, Betty Y. S.
- Lee, L. James
- ORCID
- Venue
- Nature Biomedical Engineering
- Published
- 2019-12-16
- Volume
- 4
- Issue
- 1
- Pages
- 69-83
- ISSN
- 2157-846X (ISSN-L)
- PMC
- PMC7080209
- OpenAlex
- W2995128645
- Cited by
- 744
- References
- 49
- Citations by year
- 2026: 81 · 2025: 125 · 2024: 135 · 2023: 128 · 2022: 117 · 2021: 101 · 2020: 56
- FWCI
- 22.67
- Type
- journal article
- Language
- en
- Update notices
- correction (2021-06-15)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Nature Biomedical Engineering (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Department of Health & Human Services | NIH | National Cancer Institute (K08CA241070)
- U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (1R01NS104315)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- exosomes, mRNA delivery, electroporation, exosome production, targeting peptides, tumor suppression, nucleic acid carriers, exosome secretion, brain tumors
- MeSH terms
- Animals, Brain Neoplasms, Cells, Cultured, Disease Models, Animal, Glioma, Humans, Mice, Inbred C57BL, RNA, Messenger, Signal Transduction, Drug Delivery Systems, Electroporation, MicroRNAs, Nanotechnology, Exosomes, HEK293 Cells
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