The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
DISARM is a widespread bacterial defence system with broad anti-phage activities
Open accessAbstract
The evolutionary pressure imposed by phage predation on bacteria and archaea has resulted in the development of effective anti-phage defence mechanisms, including restriction–modification and CRISPR–Cas systems. Here, we report on a new defence system, DISARM (defence island system associated with restriction–modification), which is widespread in bacteria and archaea. DISARM is composed of five genes, including a DNA methylase and four other genes annotated as a helicase domain, a phospholipase D (PLD) domain, a DUF1998 domain and a gene of unknown function. Engineering the Bacillus paralicheniformis 9945a DISARM system into Bacillus subtilis has rendered the engineered bacteria protected against phages from all three major families of tailed double-stranded DNA phages. Using a series of gene deletions, we show that four of the five genes are essential for DISARM-mediated defence, with the fifth (PLD) being redundant for defence against some of the phages. We further show that DISARM restricts incoming phage DNA and that the B. paralicheniformis DISARM methylase modifies host CCWGG motifs as a marker of self DNA akin to restriction–modification systems. Our results suggest that DISARM is a new type of multi-gene restriction–modification module, expanding the arsenal of defence systems known to be at the disposal of prokaryotes against their viruses. A widespread anti-phage defence system, DISARM (defence island system associated with restriction–modification), is identified.
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Cites
Cutadapt removes adapter sequences from high-throughput sequencing reads
Martin, M. (2011) · EMBnet.journal · doi:10.14806/ej.17.1.200 · Cited by 33,889
Robinson, J. T., Thorvaldsdóttir, H., Winckler, W., Guttman, M., Lander, E. S., Getz, G., & Mesirov, J. P. (2011) · Nature Biotechnology · doi:10.1038/nbt.1754 · Cited by 15,390
The Phyre2 web portal for protein modeling, prediction and analysis
Kelley, L. A., Mezulis, S., Yates, C. M., Wass, M. N., & Sternberg, M. J. E. (2015) · Nature Protocols · doi:10.1038/nprot.2015.053 · Cited by 9,652
Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications
Krueger, F., & Andrews, S. R. (2011) · Bioinformatics · doi:10.1093/bioinformatics/btr167 · Cited by 5,297
Bacteriophage resistance mechanisms
Labrie, S. J., Samson, J. E., & Moineau, S. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2315 · Cited by 2,397
Deatherage, D. E., & Barrick, J. E. (2014) · Methods in Molecular Biology · doi:10.1007/978-1-4939-0554-6_12 · Cited by 1,609
IMG: the integrated microbial genomes database and comparative analysis system
Markowitz, V. M., Chen, I.-M. A., Palaniappan, K., Chu, K., Szeto, E., Grechkin, Y., Ratner, A., Jacob, B., Huang, J., Williams, P., et al. (2012) · Nucleic Acids Research · doi:10.1093/nar/gkr1044 · Cited by 1,311
REBASE—a database for DNA restriction and modification: enzymes, genes and genomes
Roberts, R. J., Vincze, T., Posfai, J., & Macelis, D. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkp874 · Cited by 991
CRISPR-Cas immunity in prokaryotes
Marraffini, L. A. (2015) · Nature · doi:10.1038/nature15386 · Cited by 829
A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
Roberts, R. J. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg274 · Cited by 755
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Self-citationMechanisms and clinical importance of bacteriophage resistance
Egido, J. E., Costa, A. R., Aparicio-Maldonado, C., Haas, P.-J., & Brouns, S. J. J. (2022) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuab048 · Cited by 325
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Metadata
- Authors
- Ofir, G., Melamed, S., Sberro, H., Mukamel, Z., Silverman, S., Yaakov, G., Doron, S., & Sorek, R.
- Venue
- Nature Microbiology
- Published
- 2017-10-30
- Volume
- 3
- Issue
- 1
- Pages
- 90-98
- ISSN
- 2058-5276 (ISSN-L)
- OpenAlex
- W2766750388
- Cited by
- 331
- References
- 39
- FWCI
- 19.64
- Type
- journal article
- Language
- en
- Open access
- green
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- Ofir, G., Melamed, S., Sberro, H., Mukamel, Z., Silverman, S., Yaakov, G., Doron, S., & Sorek, R. (2017). DISARM is a widespread bacterial defence system with broad anti-phage activities. Nature Microbiology. https://doi.org/10.1038/s41564-017-0051-0
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