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Differential bacterial capture and transport preferences facilitate co-growth on dietary xylan in the human gut

Leth, Maria Louise, Ejby, Morten, Workman, Christopher, Ewald, David Adrian, Pedersen, Signe Schultz, Sternberg, Claus, Bahl, Martin Iain, Licht, Tine Rask, Aachmann, Finn Lillelund, Westereng, Bjørge, Abou Hachem, Maher·2018·Nature Microbiology
DOI 10.1038/s41564-018-0132-8
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Abstract

Metabolism of dietary glycans is pivotal in shaping the human gut microbiota. However, the mechanisms that promote competition for glycans among gut commensals remain unclear. Roseburia intestinalis, an abundant butyrate-producing Firmicute, is a key degrader of the major dietary fibre xylan. Despite the association of this taxon to a healthy microbiota, insight is lacking into its glycan utilization machinery. Here, we investigate the apparatus that confers R. intestinalis growth on different xylans. R. intestinalis displays a large cell-attached modular xylanase that promotes multivalent and dynamic association to xylan via four xylan-binding modules. This xylanase operates in concert with an ATP-binding cassette transporter to mediate breakdown and selective internalization of xylan fragments. The transport protein of R. intestinalis prefers oligomers of 4-5 xylosyl units, whereas the counterpart from a model xylan-degrading Bacteroides commensal targets larger ligands. Although R. intestinalis and the Bacteroides competitor co-grew in a mixed culture on xylan, R. intestinalis dominated on the preferred transport substrate xylotetraose. These findings highlight the differentiation of capture and transport preferences as a possible strategy to facilitate co-growth on abundant dietary fibres and may offer a unique route to manipulate the microbiota based on glycan transport preferences in therapeutic interventions to boost distinct taxa.

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Metadata

Authors
Leth, M. L., Ejby, M., Workman, C., Ewald, D. A., Pedersen, S. S., Sternberg, C., Bahl, M. I., Licht, T. R., Aachmann, F. L., Westereng, B., & Abou Hachem, M.
Venue
Nature Microbiology
Publisher
Springer Science and Business Media LLC
Published
2018-04-02
Volume
3
Issue
5
Pages
570-580
ISSN
2058-5276
DOI
10.1038/s41564-018-0132-8
Cited by
167
References
11
Language
en
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