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Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Open accessAbstract
Applications of adenine base editors (ABEs) have been constrained by the limited compatibility of the deoxyadenosine deaminase component with Cas homologs other than SpCas9. We evolved the deaminase component of ABE7.10 using phage-assisted non-continuous and continuous evolution (PANCE and PACE), which resulted in ABE8e. ABE8e contains eight additional mutations that increase activity (k app) 590-fold compared with that of ABE7.10. ABE8e offers substantially improved editing efficiencies when paired with a variety of Cas9 or Cas12 homologs. ABE8e is more processive than ABE7.10, which could benefit screening, disruption of regulatory regions and multiplex base editing applications. A modest increase in Cas9-dependent and -independent DNA off-target editing, and in transcriptome-wide RNA off-target editing can be ameliorated by the introduction of an additional mutation in the TadA-8e domain. Finally, we show that ABE8e can efficiently install natural mutations that upregulate fetal hemoglobin expression in the BCL11A enhancer or in the the HBG promoter in human cells, targets that were poorly edited with ABE7.10. ABE8e augments the effectiveness and applicability of adenine base editing.
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“ABE8e has an “editing window,” the nucleotides accessible for deamination, of positions 4–9 in the protospacer (11–16 bases upstream from the PAM).16”
From Adenine base editor for knockout of proteins: A practical guide from design to analysis with updated MultiEditRbatch (2026) · cites this paper as [16]
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“Nevertheless, accumulative studies have proved that sgRNA-independent off-target effects also exist, urging unbiased experimental detection and validation (Richter et al., 2020; O'Geen et al., 2015).”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Richter et al., 2020
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“The sgRNA-independent DNA editing is likely deposited on loci where DNA unwinds into an R-loop so single-strand genomic DNA is exposed to deaminases”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Richter et al., 2020
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“ABE8e, a variant published in 2020, exhibits a 590-fold increase in activity due to numerous mutations in the TadA deaminase domain.16”
From Adenine base editor for knockout of proteins: A practical guide from design to analysis with updated MultiEditRbatch (2026) · cites this paper as [16]
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“Truncated gRNA: truncated by 2-3bp at 5’ end | Decreased off-target effects”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [72]
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“Extended gRNA: GGX20 | Decreased off-target effects”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [72]
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Self-citationShrestha, S., & Offer, S. M. (2016) · Medical Epigenetics · doi:10.1159/000444713 · Cited by 8,951
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437
Self-citationProgrammable base editing of A•T to G•C in genomic DNA without DNA cleavage
Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017) · Nature · doi:10.1038/nature24644 · Cited by 4,184
Self-citationClinVar: public archive of relationships among sequence variation and human phenotype
Landrum, M. J., Lee, J. M., Riley, G. R., Jang, W., Rubinstein, W. S., Church, D. M., & Maglott, D. R. (2014) · Nucleic Acids Research · doi:10.1093/nar/gkt1113 · Cited by 3,516
ClinVar: public archive of interpretations of clinically relevant variants
Landrum, M. J., Lee, J. M., Benson, M., Brown, G., Chao, C., Chitipiralla, S., Gu, B., Hart, J., Hoffman, D., Hoover, J., et al. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkv1222 · Cited by 2,758
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
Tsai, S. Q., Zheng, Z., Nguyen, N. T., Liebers, M., Topkar, V. V., Thapar, V., Wyvekens, N., Khayter, C., Iafrate, A. J., Le, L. P., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3117 · Cited by 2,268
Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing
Liu, Z., Lu, Z., Yang, G., Huang, S., Li, G., Feng, S., Liu, Y., Li, J., Yu, W., Zhang, Y., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-04768-7 · Cited by 2,103
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Rees, H. A., & Liu, D. R. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0059-1 · Cited by 1,675
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Items 501–600 of 1,000
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Progression and application of CRISPR-Cas genomic editors
Yang, L., Tang, J., Ma, X., Lin, Y., Ma, G., Shan, M., Wang, L., & Yang, Y. (2021) · Methods · doi:10.1016/j.ymeth.2021.03.013 · Cited by 10
Highly efficient A-to-G base editing by ABE8.17 in rabbits
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Zhang, Z., Tao, W., Huang, S., Sun, W., Wang, Y., Jiang, W. G., Huang, X., & Lin, C. (2022) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2022.07.026 · Cited by 11
Verhagen, H. J. M. P., Kuijk, C., Rutgers, L., Kokke, A. M., van der Meulen, S. A., van Mierlo, G. J., Voermans, C., & van den Akker, E. (2022) · The CRISPR Journal · doi:10.1089/crispr.2021.0112 · Cited by 10
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Li, J., Liang, Q., Zhou, H., Zhou, M., & Huang, H. (2023) · Cellular & Molecular Biology Letters · doi:10.1186/s11658-023-00454-9 · Cited by 10
Yang, L., Liu, Z., Sun, J., Chen, Z., Gao, F., & Guo, Y. (2024) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.18145 · Cited by 10
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Park, J.-C., Kim, K.-T., Jang, H., & Cha, H. (2023) · International Journal of Stem Cells · doi:10.15283/ijsc22171 · Cited by 9
Böck, D., Revers, I. M., Bomhof, A. S. J., Hillen, A. E. J., Boeijink, C., Kissling, L., Egli, S., Moreno-Mateos, M. A., van der Knaap, M. S., van Til, N. P., et al. (2024) · Molecular Therapy · doi:10.1016/j.ymthe.2024.03.009 · Cited by 9
Li, Y., Li, S., Li, C., Zhang, C., Yan, L., Li, J., He, Y., Guo, Y. J., & Xia, L. (2024) · aBIOTECH · doi:10.1007/s42994-024-00138-8 · Cited by 9
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Zhong, Z., Hu, X., Zhang, R., Liu, X., Chen, W., Zhang, S., Sun, J., & Zhong, T. P. (2024) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2024.10.006 · Cited by 9
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Rose, I., Greenwood, M., Biggart, M., Baumlin, N., Tarran, R., Hart, S. L., & Baines, D. L. (2025) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-025-05587-y · Cited by 10
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Kaplan, E. L., Steger, R. J., Shah, S. T., Drepanos, L. M., Griffith, A. L., Reint, G., & Doench, J. G. (2025) · Nature Genetics · doi:10.1038/s41588-025-02366-0 · Cited by 9
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Basit, A., Zhu, J., & Zheng, W. (2026) · Archives of Microbiology · doi:10.1007/s00203-025-04687-4 · Cited by 11
Kakhki, M. P., Rangani, F., Ewing, E., Cucuzza, C. S., Zheleznyakova, G. Y., Kalomoiri, M., Kenny, L., Raghavan, A., Prakash, C. R., van den Hoeven, G., et al. (2026) · Genome biology · doi:10.1186/s13059-026-03967-6 · Cited by 12
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Zhao, D., Gao, X., Zhou, J., Li, J., Qian, Y., Wang, D., Niu, W., Zhang, T., Hu, M., Xiong, H., et al. (2023) · BMC Biology · doi:10.1186/s12915-023-01754-4 · Cited by 9
Nandy, K., Babu, D., Rani, S., Joshi, G., Ijee, S., George, A., Palani, D., Premkumar, C., Rajesh, P., Vijayanand, S., et al. (2023) · Scientific Reports · doi:10.1038/s41598-023-42174-2 · Cited by 10
Li, P., Dong, D., Gao, F., Xie, Y., Huang, H., Sun, S., Ma, Z., He, C., Lai, J., Du, X., et al. (2024) · Science China Life Sciences · doi:10.1007/s11427-023-2682-5 · Cited by 9
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Bellingrath, J.-S., McClements, M. E., Kaukonen, M. K., Fischer, M. D., & MacLaren, R. E. (2021) · Genes · doi:10.3390/genes12121908 · Cited by 9
Zhou, X., Gao, J., Luo, L., Huang, C., Wu, J., & Wang, X. (2024) · Communications Biology · doi:10.1038/s42003-024-07078-5 · Cited by 11
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Ji, J. H., Armstrong, W. R., Li, J. H., Lalani, A., Lee, C. D., Bharadwaj, V. S., Ho, K., Liang, J., Muir, A., & O’Sullivan, T. E. (2026) · Immunity · doi:10.1016/j.immuni.2026.01.017 · Cited by 8
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Huang, K., Tian, J., Zhao, W., Zhang, S., Xiang, L., Yang, M., Ls, L., Huang, Y., Zhang, N., Sun, L., et al. (2026) · iMetaOmics. · doi:10.1002/imo2.70115 · Cited by 8
Chakravorty, M., Nanda, M., Arora, N., Singh, S., Kumar, V., & Deshwal, S. (2022) · Plant Gene · doi:10.1016/j.plgene.2022.100369 · Cited by 9
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Cha, J. H., Lee, S., Yun, Y., Choi, W. H., Koo, H., Jung, S. H., Chae, H. B., Lee, S. J., Lee, S. J., Jo, D. H., et al. (2024) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2024.102257 · Cited by 7
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Sun, Y., Qu, K., Corsi, G. I., Anthon, C., Pan, X., Xiang, X., Jensen, L. J., Lin, L., Luo, Y., & Gorodkin, J. (2025) · Nature Communications · doi:10.1038/s41467-025-65200-5 · Cited by 7
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Umbach, A., Maule, G., Kheir, E., Cutarelli, A., Foglia, M., Guarrera, L., Fava, L. L., Conti, L., Garattini, E., Terao, M., et al. (2022) · Stem Cell Research & Therapy · doi:10.1186/s13287-022-03135-0 · Cited by 7
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Feng, Q., Li, Q., Zhou, H., Wang, Z., Lin, C., Jiang, Z., Liu, T., & Wang, D. (2024) · MedComm · doi:10.1002/mco2.672 · Cited by 8
CRISPR-Cas System-Mediated Genetic Modification in Bacillus spp.: Current Status and Future
Wu, J., Zheng, S., Wang, L., Xia, X., Li, F., Lv, M.-L., Wu, Q., Huang, Z., & Miao, H. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5c03140 · Cited by 7
Sidorova, O. A., Sayed, S., Paszkowski‐Rogacz, M., Seifert, M., Camgöz, A., Roeder, I., Bornhäuser, M., Thiede, C., & Buchholz, F. (2022) · Cells · doi:10.3390/cells11050854 · Cited by 7
New CRISPR Tools to Correct Pathogenic Mutations in Usher Syndrome
Major, L., McClements, M. E., & MacLaren, R. E. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231911669 · Cited by 8
Gao, Z., Jiang, W., Zhang, Y., Zhang, L., Yi, M., Wang, H., Ma, Z., Qu, B., Ji, X., Long, H., et al. (2023) · Communications Biology · doi:10.1038/s42003-023-05134-0 · Cited by 6
Doering, L., Cornean, A., Thumberger, T., Benjaminsen, J., Wittbrodt, B., Kellner, T., Hammouda, O. T., Gorenflo, M., Wittbrodt, J., & Gierten, J. (2023) · Disease Models & Mechanisms · doi:10.1242/dmm.049811 · Cited by 6
Zhang, G., Song, Z., Huang, S., Wang, Y., Sun, J., Qiao, L., Li, G., Feng, Y., Han, W., Tang, J., et al. (2024) · Advanced Science · doi:10.1002/advs.202405426 · Cited by 7
Harnessing the power of directed evolution to improve genome editing systems
Su, Q., Zhou, M., Cheng, C., & Niu, J. (2021) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2021.02.004 · Cited by 8
Mathis, N., Allam, A., Tálas, A., Benvenuto, E., Schep, R., Damodharan, T., Balázs, Z., Janjuha, S., Schmidheini, L., Böck, D., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.10.09.561414 · Cited by 7
Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing
Carrozzo, I., Maule, G., Gentile, C., Umbach, A., Ciciani, M., Guidone, D., Santis, M. D., Petris, G., Galietta, L. J. V., Arosio, D., et al. (2025) · Molecular Therapy · doi:10.1016/j.ymthe.2025.01.011 · Cited by 6
Genome editing with programmable base editors in human cells
Osgood, N. R. B., Zawalick, N. M., Sawyer, C. B., Cowan, Q. T., Gu, S., Mawson, S. J., Ranzau, B. L., Li, L., Gymrek, M., Goren, A., et al. (2025) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2025.01.001 · Cited by 6
Delivery of genome editors with engineered virus-like particles
Lu, C., Li, Y., Cummings, J. R., & Banskota, S. (2025) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2025.01.007 · Cited by 8
Advanced research and exploration of CRISPR technology in the field of directed evolution
Long, R., Tang, D., Yang, T., Li, M., Lu, Y., Liu, W., & Jiang, L. (2025) · Biotechnology Advances · doi:10.1016/j.biotechadv.2025.108633 · Cited by 6
Engraftment and persistence of HBB base-edited hematopoietic stem cells in nonhuman primates
Radtke, S., Fields, E., Swing, K., Kanestrom, G., Yen, J. S., Pande, D., Enstrom, M., Humbert, O., Weiss, M. J., Liu, D. R., et al. (2025) · Science Translational Medicine · doi:10.1126/scitranslmed.adn2601 · Cited by 6
Self-citationBioFuse: A programmable timer switch of gene expression
Huang, C., Zhao, J., Tan, Z., Dai, S., Wang, B., Xie, Z., Zhang, F., Zhou, Y., Yu, N., Cai, C., et al. (2025) · Science Advances · doi:10.1126/sciadv.adv7892 · Cited by 6
CRISPR-Cas technologies: Emerging tools from research to clinical application
Hyeon, H., Hwang, S., Luo, Y., Shin, E., Yeom, J., Kim, H., Ryu, M., & Lee, K. (2025) · The Journal of Microbiology · doi:10.71150/jm.2504012 · Cited by 7
AI-guided Cas9 engineering provides an effective strategy to enhance base editing
Wei, D., Cheng, P., Song, Z., Liu, Y., Xu, X., Huang, X., Wang, X., Zhang, Y., Shu, W., & Wei, Y. (2025) · Molecular Systems Biology · doi:10.1038/s44320-025-00142-0 · Cited by 6
Kansal, R. (2025) · Genes · doi:10.3390/genes16111367 · Cited by 6
Guarding the genome: strategies for off-target detection and minimization in CRISPR/Cas9 system
Lotfi, M., Yazdi, Z. F., Hashemi, M. R., Abbaszadegan, M. R., & Sharif, S. (2025) · Expert Review of Molecular Diagnostics · doi:10.1080/14737159.2025.2581677 · Cited by 6
PRICE: direct and robust detection of microRNAs at single-nucleotide resolution
Wang, B., Zhou, S., Zhang, X., Wang, R., Huang, S., Li, A., He, H., Zhang, Y., Wei, Y., Yang, Z., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-69181-x · Cited by 6
A proteome-wide dependency map of protein interaction motifs
Ambjørn, S. M., Meeusen, B., Kliche, J., Wang, J., Garvanska, D. H., Kruse, T., Mendez, B. L., Mann, M., Mailand, N., Hertz, E. P. T., et al. (2026) · Nature Structural & Molecular Biology · doi:10.1038/s41594-026-01762-2 · Cited by 8
Precise DNA base editing using AlphaFold3-based contact modelling
Meng, H., Lei, Z., Yan, Y., Wang, L., Zhang, S., Rao, X., Shao, C., Zhang, X., Chen, K., Yang, L., et al. (2026) · Nature · doi:10.1038/s41586-026-10794-z · Cited by 6
Ting, W., & Ng, I. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.3c00344 · Cited by 6
Wang, Z., Yuan, H., Yang, L., Ma, L., Zhang, Y., Deng, J., Li, X., Xiao, W., Li, Z., Qiu, J., et al. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.127418 · Cited by 5
Zhang, L., Li, K., Liu, Z., An, L., Wei, H., Pang, S., Cao, Z., Huang, X., Jin, X., & Ma, X. (2024) · Molecular Therapy · doi:10.1016/j.ymthe.2024.03.028 · Cited by 6
Hsiao, S., Chen, S., Jiang, Y., Wang, Q., Yang, Y., Lai, Y., Zhong, T., Liao, J., & Wu, Y. (2024) · Advanced Science · doi:10.1002/advs.202309004 · Cited by 7
In vivo CRISPR base editing for treatment of Huntington’s disease
Shirguppe, S., Gapinske, M., Swami, D., Shenouda, K., Miskalis, A., Gosstola, N. C., Siller, D. D. B., Guerra, I., Acharya, P., Joulani, D., et al. (2026) · Nature Biomedical Engineering · doi:10.1038/s41551-026-01747-y · Cited by 8
The era of genome editing: current updates and the status of food and nutrition security
Khaled, F., Mehta, S., Agrawal, P. K., Abdel-Monem, U. M., Elakkad, H. A., Gendy, A. S. H., Al-Maracy, S. H. A., Heakel, R. M. Y., Wafa, H. A., Fathy, R., et al. (2024) · Frontiers in Sustainability · doi:10.3389/frsus.2024.1378712 · Cited by 6
Metadata
- Authors
- Richter, M. F., Zhao, K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer, D. E., Doudna, J. A., & Liu, D. R.
- Affiliations
- Broad Institute (US)
- Boston Children's Hospital (US)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Liu, David R.
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2020-03-16
- Volume
- 38
- Issue
- 7
- Pages
- 883-891
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- PMC
- PMC7357821
- OpenAlex
- W3012327875
- Cited by
- 1,020
- References
- 60
- Citations by year
- 2026: 158 · 2025: 196 · 2024: 192 · 2023: 192 · 2022: 148 · 2021: 103 · 2020: 29
- FWCI
- 38.37
- Type
- journal article
- Language
- en
- Update notices
- correction (2020-05-20)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- Funders
- U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (P01HL053749)
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases (U01 AI142756)
- U.S. Department of Health & Human Services | NIH | National Human Genome Research Institute (RM1 HG009490)
- U.S. Department of Health & Human Services | NIH | National Institute of Biomedical Imaging and Bioengineering (R01 EB022376)
- U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (R35 GM118062)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Keywords
- adenine base editing, ABE8e, phage-assisted continuous evolution, Cas12, processivity, off-target editing, fetal hemoglobin, PAM site
- MeSH terms
- Gene Editing, Adenine, Adenosine Deaminase, Bacteriophages, DNA, Humans, Mutation, RNA, Mutagenesis, HEK293 Cells, CRISPR-Cas Systems
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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