Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

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Epigenetic Regulations of GABAergic Neurotransmission: Relevance for Neurological Disorders and Epigenetic Therapy

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Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

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ClinVar: public archive of interpretations of clinically relevant variants

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GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Tsai, S. Q., Zheng, Z., Nguyen, N. T., Liebers, M., Topkar, V. V., Thapar, V., Wyvekens, N., Khayter, C., Iafrate, A. J., Le, L. P., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3117 · Cited by 2,268

Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing

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Base editing: precision chemistry on the genome and transcriptome of living cells

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CRISPResso2 provides accurate and rapid genome editing sequence analysis

Clement, K., Rees, H. A., Canver, M. C., Gehrke, J. M., Farouni, R., Hsu, J. Y., Cole, M. A., Liu, D. R., Joung, J. K., Bauer, D. E., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0032-3 · Cited by 1,656

PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics

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Helicase-assisted continuous editing for programmable mutagenesis of endogenous genomes

Chen, X. D., Chen, Z., Wythes, G., Zhang, Y., Orr, B. C., Sun, G., Chao, Y.-K., Torres, A. N., Thao, K., Vallurupalli, M., et al. (2024) · Science · doi:10.1126/science.adn5876 · Cited by 47

Emerging applications of gene editing technologies for the development of climate-resilient crops

Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 55

CRISPR-Cas9 base editing of pathogenic CaMKIIδ improves cardiac function in a humanized mouse model

Lebek, S., Caravia, X. M., Straub, L. G., Alzhanov, D., Tan, W. H., Li, H. Z., McAnally, J., Chen, K., Xu, L., Scherer, P. E., et al. (2023) · Journal of Clinical Investigation · doi:10.1172/jci175164 · Cited by 46

Efficient and high-fidelity base editor with expanded PAM compatibility for cytidine dinucleotide

Liu, Z., Chen, S., Jia, Y., Shan, H., Chen, M., Song, Y., Lai, L., & Li, Z. (2021) · Science China Life Sciences · doi:10.1007/s11427-020-1775-2 · Cited by 35

Universal toxin-based selection for precise genome engineering in human cells

Li, S., Akrap, N., Cerboni, S., Porritt, M. J., Wimberger, S., Lundin, A., Möller, C., Firth, M. A., Gordon, E., Lazovic, B., et al. (2021) · Nature Communications · doi:10.1038/s41467-020-20810-z · Cited by 46

Systems for in vivo hypermutation: a quest for scale and depth in directed evolution

Rix, G., & Liu, C. C. (2021) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2021.02.008 · Cited by 43

SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e

Kulcsár, P. I., Tálas, A., Ligeti, Z., Krausz, S. L., & Welker, E. (2022) · Nature Communications · doi:10.1038/s41467-022-34527-8 · Cited by 46

Gene Therapy and Gene Editing for β-Thalassemia

Christakopoulos, G. E., Telange, R., Yen, J. S., & Weiss, M. J. (2023) · Hematology/Oncology Clinics of North America · doi:10.1016/j.hoc.2022.12.012 · Cited by 43

Artificial evolution of OsEPSPS through an improved dual cytosine and adenine base editor generated a novel allele conferring rice glyphosate tolerance

Zhang, C., Zhong, X., Li, S., Yan, L., Li, J., He, Y., Lin, Y., Zhang, Y., & Xia, L. (2023) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13543 · Cited by 42

A cytosine base editor toolkit with varying activity windows and target scopes for versatile gene manipulation in plants

Xiong, X., Li, Z., Liang, J., Liu, K., Li, C., & Li, J.-F. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac166 · Cited by 41

Machine learning-coupled combinatorial mutagenesis enables resource-efficient engineering of CRISPR-Cas9 genome editor activities

Thean, D., Chu, H. Y., Fong, J. H. C., Chan, B. K. C., Zhou, P., Kwok, C. C. S., Chan, Y. M., Mak, S. Y. L., Choi, G. C. G., Ho, J. W. K., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-29874-5 · Cited by 44

Reconstructed glycosylase base editors GBE2.0 with enhanced C-to-G base editing efficiency and purity

Sun, N., Zhao, D., Li, S., Zhang, Z., Bi, C., & Zhang, X. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2022.03.023 · Cited by 40

The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives

Li, J., Yu, X., Zhang, C., Li, N., & Zhao, J. (2022) · aBIOTECH · doi:10.1007/s42994-022-00076-3 · Cited by 42

Rescue of hearing by adenine base editing in a humanized mouse model of Usher syndrome type 1F

Peters, C. W., Hanlon, K. S., Ivanchenko, M. V., Zinn, E. M., Linarte, E., Li, Y., Levy, J. M., Liu, D. R., Kleinstiver, B. P., Indzhykulian, A. A., et al. (2023) · Molecular Therapy · doi:10.1016/j.ymthe.2023.06.007 · Cited by 36

Elimination of CaMKIIδ Autophosphorylation by CRISPR-Cas9 Base Editing Improves Survival and Cardiac Function in Heart Failure in Mice

Lebek, S., Caravia, X. M., Chemello, F., Tan, W. H., McAnally, J., Chen, K., Xu, L., Liu, N., Bassel‐Duby, R., & Olson, E. N. (2023) · Circulation · doi:10.1161/circulationaha.123.065117 · Cited by 44

Engineering IscB to develop highly efficient miniature editing tools in mammalian cells and embryos

Xue, N., Hong, D., Zhang, D., Wang, Q., Zhang, S., Yang, L., Chen, X., Li, Y., Han, H., Hu, C., et al. (2024) · Molecular Cell · doi:10.1016/j.molcel.2024.07.007 · Cited by 45

Multiplexed assays of variant effect for clinical variant interpretation

McEwen, A. E., Tejura, M., Fayer, S., Starita, L. M., & Fowler, D. M. (2025) · Nature Reviews Genetics · doi:10.1038/s41576-025-00870-x · Cited by 52

Marker-free co-selection for successive rounds of prime editing in human cells

Levesque, S., Mayorga, D., Fiset, J.-P., Goupil, C., Duringer, A., Loiselle, A., Bouchard, E., Agudelo, D., & Doyon, Y. (2022) · Nature Communications · doi:10.1038/s41467-022-33669-z · Cited by 41

A most formidable arsenal: genetic technologies for building a better mousePreprint

Clark, J. F., Dinsmore, C. J., & Soriano, P. M. (2020) · Genes & Development · doi:10.1101/gad.342089.120 · Cited by 40

Improving adenine and dual base editors through introduction of TadA-8e and Rad51DBD

Xue, N., Liu, X., Zhang, D., Wu, Y., Zhong, Y., Wang, J., Fan, W., Jiang, H., Zhu, B., Ge, X., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-36887-1 · Cited by 39

The Design and Application of DNA-Editing Enzymes as Base Editors

Rallapalli, K. L., & Komor, A. C. (2023) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-052521-013938 · Cited by 44

Base editor screens for in situ mutational scanning at scale

Lue, N. Z., & Liau, B. B. (2023) · Molecular Cell · doi:10.1016/j.molcel.2023.06.009 · Cited by 43

Mapping variant effects on anti-tumor hallmarks of primary human T cells with base-editing screens

Walsh, Z. H., Shah, P., Kothapalli, N., Shah, S. B., Nikolényi, G., Brodtman, D. Z., Leuzzi, G., Rogava, M., Mu, M., Ho, P., et al. (2024) · Nature Biotechnology · doi:10.1038/s41587-024-02235-x · Cited by 42

Engineered IscB–ωRNA system with expanded target range for base editing

Xiao, Q., Li, G., Han, D., Wang, H., Yao, M., Ma, T., Zhou, J., Zhang, Y., Zhang, X., He, B., et al. (2024) · Nature Chemical Biology · doi:10.1038/s41589-024-01706-1 · Cited by 41

A Tale of Two Moieties: Rapidly Evolving CRISPR/Cas-Based Genome Editing

Yang, L. C., & Chen, J. (2020) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2020.06.003 · Cited by 38

Human cell based directed evolution of adenine base editors with improved efficiency

Fu, J., Li, Q., Liu, X., Tu, T., Lv, X., Yin, X., Lv, J., Song, Z., Qu, J., Zhang, J., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-26211-0 · Cited by 35

mRNA-mediated delivery of gene editing tools to human primary muscle stem cells

Stadelmann, C., Francescantonio, S. D., Marg, A., Müthel, S., Spuler, S., & Escobar, H. (2022) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2022.02.016 · Cited by 39

A split and inducible adenine base editor for precise in vivo base editing

Zeng, H., Yuan, Q., Peng, F., Ma, D., Lingineni, A., Chee, K., Gilberd, P., Osikpa, E. C., Sun, Z., & Gao, X. (2023) · Nature Communications · doi:10.1038/s41467-023-41331-5 · Cited by 37

Revolutionising healing: Gene Editing's breakthrough against sickle cell disease

Dimitrievska, M., Bansal, D., Vitale, M., Strouboulis, J., Miccio, A., Nicolaides, K. H., Hoss, S. E., Shangaris, P., & Jackow, J. (2024) · Blood Reviews · doi:10.1016/j.blre.2024.101185 · Cited by 41

Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination

Li, S., Yuan, B., Cao, J., Chen, J., Chen, J., Qiu, J., Zhao, X., Wang, X., Qiu, Z., & Cheng, T. (2020) · Nature Communications · doi:10.1038/s41467-020-19730-9 · Cited by 35

CRISPR–Cas12a base editors confer efficient multiplexed genome editing in rice

Cheng, Y., Zhang, Y., Li, G., Fang, H., Sretenovic, S., Fan, A., Li, J., Xu, J., Que, Q., & Qi, Y. (2023) · Plant Communications · doi:10.1016/j.xplc.2023.100601 · Cited by 36

Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors

Chen, F., Lian, M., Ma, B., Gou, S., Luo, X., Yang, K., Shi, H., Xie, J., Ge, W., Ouyang, Z., et al. (2022) · Communications Biology · doi:10.1038/s42003-022-04152-8 · Cited by 36

Quadruple adenine base–edited allogeneic CAR T cells outperform CRISPR/Cas9 nuclease–engineered T cells

Engel, N. W., Steinfeld, I., Ryan, D., Anupindi, K., Kim, S., Wellhausen, N., Chen, L., Wilkins, K., Baker, D. J., Rommel, P. C., et al. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2427216122 · Cited by 39

Precise in vivo functional analysis of DNA variants with base editing using ACEofBASEs target prediction

Cornean, A., Gierten, J., Welz, B., Mateo, J. L., Thumberger, T., & Wittbrodt, J. (2022) · eLife · doi:10.7554/elife.72124 · Cited by 35

A large-scale genome and transcriptome sequencing analysis reveals the mutation landscapes induced by high-activity adenine base editors in plants

Li, S., Liu, L., Sun, W., Zhou, X., & Zhou, H. (2022) · Genome biology · doi:10.1186/s13059-022-02618-w · Cited by 35

Current advances of CRISPR-Cas technology in cell therapy

Qiu, H.-Y., Ji, R.-J., & Zhang, Y. (2022) · Cell Insight · doi:10.1016/j.cellin.2022.100067 · Cited by 38

TadA reprogramming to generate potent miniature base editors with high precision

Zhang, S., Song, L., Yuan, B., Zhang, C., Cao, J., Chen, J., Qiu, J., Tai, Y., Chen, J., Qiu, Z., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-36004-2 · Cited by 40

Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic mice

Yan, N., Feng, H., Sun, Y., Xin, Y., Zhang, H., Lu, H., He, C., He, C., Zuo, Z., Yuan, T., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-37508-7 · Cited by 37

Assessing and engineering the IscB–ωRNA system for programmed genome editing

Yan, H., Tan, X., Zou, S., Sun, Y., Ke, A., & Tang, W. (2024) · Nature Chemical Biology · doi:10.1038/s41589-024-01669-3 · Cited by 36

Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted ωRNAs

Marquart, K. F., Mathis, N., Mollaysa, A., Müller, S., Kissling, L., Rothgangl, T., Schmidheini, L., Kulcsár, P. I., Allam, A., Kaufmann, M. M., et al. (2024) · Nature Methods · doi:10.1038/s41592-024-02418-z · Cited by 41

The road toward AAV-mediated gene therapy of Duchenne muscular dystrophy

Bengtsson, N. E., Tasfaout, H., & Chamberlain, J. S. (2025) · Molecular Therapy · doi:10.1016/j.ymthe.2025.03.065 · Cited by 36

CRISPR/Cas Technologies and Their Applications in Escherichia coli

Dong, H., Cui, Y., & Zhang, D. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.762676 · Cited by 35

Strategies for High-Efficiency Mutation Using the CRISPR/Cas System

Feng, S., Wang, Z., Li, A., Xie, X., Liu, J., Li, S., Li, Y., Wang, B., Hu, L., Yang, L., et al. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.803252 · Cited by 40

Selective Xi reactivation and alternative methods to restore MECP2 function in Rett syndrome

Grimm, N.-B., & Lee, J. T. (2022) · Trends in Genetics · doi:10.1016/j.tig.2022.01.007 · Cited by 34

DNA topology regulates PAM-Cas9 interaction and DNA unwinding to enable near-PAMless cleavage by thermophilic Cas9

Shi, Y.-J., Duan, M., Ding, J., Wang, F.-Q., Bi, L.-L., Zhang, C.-X., Zhang, Y., Duan, J.-Y., Huang, A.-H., Lei, X., et al. (2022) · Molecular Cell · doi:10.1016/j.molcel.2022.09.032 · Cited by 35

An engineered xCas12i with high activity, high specificity and broad PAM range

Zhang, H., Kong, X., Xue, M., Hu, J., Wang, Z., Wei, Y., Wang, H., Zhou, J., Zhang, W., Xu, M., et al. (2022) · Protein & Cell · doi:10.1093/procel/pwac052 · Cited by 35

Functional restoration of a CFTR splicing mutation through RNA delivery of CRISPR adenine base editor

Amistadi, S., Maule, G., Ciciani, M., Ensinck, M. M., Keersmaecker, L. D., Ramalho, A. S., Guidone, D., Buccirossi, M., Galietta, L. J. V., Carlon, M. S., et al. (2023) · Molecular Therapy · doi:10.1016/j.ymthe.2023.03.004 · Cited by 35

Enhancing prime editor activity by directed protein evolution in yeast

Weber, Y., Böck, D., Ivascu, A., Mathis, N., Rothgangl, T., Ioannidi, E. I., Blaudt, A. C., Tidecks, L., Vadovics, M., Muramatsu, H., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-46107-z · Cited by 37

Optimizing CRISPR/Cas9 precision: Mitigating off-target effects for safe integration with photodynamic and stem cell therapies in cancer treatment

Merlin, J. P. J., & Abrahamse, H. (2024) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2024.117516 · Cited by 35

Evolution-guided protein design of IscB for persistent epigenome editing in vivo

Kannan, S., Altae-Tran, H., Zhu, S., Xu, P., Strebinger, D., Oshiro, R., Faure, G., Moeller, L., Pham, J., Mears, K. S., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-025-02655-3 · Cited by 40

Synthetic evolution of herbicide resistance using a T7 RNAP–based random DNA base editor

Butt, H., Moreno, J. L., & Mahfouz, M. M. (2022) · Life Science Alliance · doi:10.26508/lsa.202201538 · Cited by 34

Nonviral base editing of KCNJ13 mutation preserves vision in a model of inherited retinal channelopathy

Kabra, M., Shahi, P. K., Wang, Y., Sinha, D., Spillane, A., Newby, G. A., Saxena, S., Tong, Y., Chang, Y., Abdeen, A. A., et al. (2023) · Journal of Clinical Investigation · doi:10.1172/jci171356 · Cited by 36

A base editor for the long-term restoration of auditory function in mice with recessive profound deafness

Cui, C., Wang, S., Wang, D., Zhao, J., Huang, B., Zhu, B., Chen, Y., Tang, H., Han, Y., Cheng, Y., et al. (2024) · Nature Biomedical Engineering · doi:10.1038/s41551-024-01235-1 · Cited by 35

Engineering Cas9 for human genome editing

Slaymaker, I. M., & Gaudelli, N. M. (2021) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2021.03.004 · Cited by 34

Diverse Approaches to Gene Therapy of Sickle Cell Disease

White, S. L., Hart, K. L., & Kohn, D. B. (2022) · Annual Review of Medicine · doi:10.1146/annurev-med-042921-021707 · Cited by 33

Improved Dual Base Editor Systems (iACBEs) for Simultaneous Conversion of Adenine and Cytosine in the Bacterium Escherichia coli

Shelake, R. M., Pramanik, D., & Kim, J. (2023) · mBio · doi:10.1128/mbio.02296-22 · Cited by 34

Reversing the Central Dogma: RNA-guided control of DNA in epigenetics and genome editing

Chang, H. Y., & Qi, L. S. (2023) · Molecular Cell · doi:10.1016/j.molcel.2023.01.010 · Cited by 33

Base editors: development and applications in biomedicine

Liang, Y., Chen, F., Wang, K., & Lai, L. (2023) · Frontiers of Medicine · doi:10.1007/s11684-023-1013-y · Cited by 33

Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification

Ryu, J., Barkal, S., Yu, T., Jankowiak, M., Zhou, Y., Francoeur, M., Phan, Q. V., Li, Z., Tognon, M., Brown, L., et al. (2024) · Nature Genetics · doi:10.1038/s41588-024-01726-6 · Cited by 33

Structure-guided discovery of highly efficient cytidine deaminases with sequence-context independence

Xu, K., Feng, H., Zhang, H., He, C., Kang, H., Yuan, T., Shi, L., Zhou, C., Hua, G., Cao, Y., et al. (2024) · Nature Biomedical Engineering · doi:10.1038/s41551-024-01220-8 · Cited by 36

ROS burst prolongs transcriptional condensation to slow shoot apical meristem maturation and achieve heat-stress resilience in tomato

Huang, X., Xiao, N., Xie, Y., & Cao, X. (2025) · Developmental Cell · doi:10.1016/j.devcel.2025.03.007 · Cited by 32

PPP2R1A mutations portend improved survival after cancer immunotherapy

Dai, Y., Knisely, A. T., Yano, M., Dang, M., Hinchcliff, E., Lee, S., Welp, A. M., Chelvanambi, M., Lastrapes, M. J., Liu, H., et al. (2025) · Nature · doi:10.1038/s41586-025-09203-8 · Cited by 32

A modular strategy for extracellular vesicle-mediated CRISPR-Cas9 delivery through aptamer-based loading and UV-activated cargo release

Elsharkasy, O. M., Hegeman, C. V., Driedonks, T. A. P., Liang, X., Lansweers, I., Cotugno, O. L., de Groot, I. Y., de Wit, Z. E. M. N. J., Garcia-Guerra, A., Moorman, N. J. A., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-65995-3 · Cited by 37

Applications and Major Achievements of Genome Editing in Vegetable Crops: A Review

Kim, Y., Kang, Y., Yang, E.-Y., Cho, M.-C., Schafleitner, R., Lee, J. H., & Jang, S. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.688980 · Cited by 33

A dual gene-specific mutator system installs all transition mutations at similar frequencies in vivo

Seo, D., Koh, B., Eom, G.-E., Kim, H. W., & Kim, S. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad266 · Cited by 32

Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors

Zhang, A., Shan, T., Sun, Y., Chen, Z., Hu, J., Hu, Z., Ming, Z., Zhu, Z., Li, X., He, J., et al. (2023) · Plant Biotechnology Journal · doi:10.1111/pbi.14156 · Cited by 34

Split complementation of base editors to minimize off-target edits

Xiong, X., Liu, K., Li, Z., Xia, F., Ruan, X.-M., He, X., & Li, J. (2023) · Nature Plants · doi:10.1038/s41477-023-01540-8 · Cited by 32

Single-swap editing for the correction of common Duchenne muscular dystrophy mutations

Chai, A. C., Chemello, F., Li, H. Z., Nishiyama, T., Chen, K., Zhang, Y., Sanchez‐Ortiz, E., Alomar, A., Xu, L., Liu, N., et al. (2023) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2023.04.009 · Cited by 32

Multimodal scanning of genetic variants with base and prime editing

Belli, O., Karava, K., Farouni, R., & Platt, R. J. (2024) · Nature Biotechnology · doi:10.1038/s41587-024-02439-1 · Cited by 34

Genome Editing for β-Hemoglobinopathies: Advances and Challenges

Frati, G., & Miccio, A. (2021) · Journal of Clinical Medicine · doi:10.3390/jcm10030482 · Cited by 31

High-throughput methods for genome editing: the more the better

Huang, Y., Shang, M., Liu, T., & Wang, K. (2022) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiac017 · Cited by 32

Direct correction of haemoglobin E β-thalassaemia using base editors

Badat, M., Ejaz, A., Peng, H., Rice, S., Zhang, W., Hentges, L. D., Fisher, C. A., Denny, N., Schweßinger, R., Yasara, N., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-37604-8 · Cited by 30

Advances in ultrahigh-throughput screening technologies for protein evolution

Liu, Z., Chen, S., & Wu, J. (2023) · Trends in biotechnology · doi:10.1016/j.tibtech.2023.03.010 · Cited by 30

Breaking genetic shackles: The advance of base editing in genetic disorder treatment

Xu, F., Zheng, C., Xu, W., Zhang, S., Liu, S., Chen, X., & Yao, K. (2024) · Frontiers in Pharmacology · doi:10.3389/fphar.2024.1364135 · Cited by 32

Generation of a high-efficiency adenine base editor with TadA8e for developing wheat dinitroaniline-resistant germplasm

Han, H., Wu, Z., Zheng, L., Han, J., Zhang, Y., Li, J., Zhang, S., Li, G., Ma, C., & Wang, P. (2021) · The Crop Journal · doi:10.1016/j.cj.2021.08.006 · Cited by 30

Construction and application of an efficient dual-base editing platform for Bacillus subtilis evolution employing programmable base conversion

Hao, W., Cui, W., Suo, F., Han, L., Cheng, Z., & Zhou, Z. (2022) · Chemical Science · doi:10.1039/d2sc05824c · Cited by 30

Direct measurement of engineered cancer mutations and their transcriptional phenotypes in single cells

Kim, H. S., Grimes, S. M., Chen, T., Sathe, A., Lau, B. T., Hwang, G., Bae, S., & Ji, H. P. (2023) · Nature Biotechnology · doi:10.1038/s41587-023-01949-8 · Cited by 31

ABE-ultramax for high-efficiency biallelic adenine base editing in zebrafish

Qin, W., Liang, F., Lin, S., Petree, C., Huang, K., Zhang, Y., Li, L., Varshney, P., Mourrain, P., Liu, Y., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-49943-1 · Cited by 30

Harnessing the evolving CRISPR/Cas9 for precision oncology

Li, T., Li, S., Kang, Y., Zhou, J., & Yi, M. (2024) · Journal of Translational Medicine · doi:10.1186/s12967-024-05570-4 · Cited by 35

Base editing repairs an SGCA mutation in human primary muscle stem cells

Escobar, H., Krause, A., Keiper, S., Kieshauer, J., Müthel, S., de Paredes, M. G., Metzler, E., Kühn, R., Heyd, F., & Spuler, S. (2021) · JCI Insight · doi:10.1172/jci.insight.145994 · Cited by 29

Phage-Assisted Continuous Evolution and Selection of Enzymes for Chemical Synthesis

Jones, K. A., Snodgrass, H. M., Belsare, K. D., Dickinson, B. C., & Lewis, J. C. (2021) · ACS Central Science · doi:10.1021/acscentsci.1c00811 · Cited by 29

Phosphonoacetate Modifications Enhance the Stability and Editing Yields of Guide RNAs for Cas9 Editors

Ryan, D. E., Diamant-Levi, T., Steinfeld, I., Taussig, D., Visal-Shah, S., Thakker, S., Lunstad, B. D., Kaiser, R. J., McCaffrey, R. M., Ortiz, M., et al. (2022) · Biochemistry · doi:10.1021/acs.biochem.1c00768 · Cited by 30

Scalable biological signal recording in mammalian cells using Cas12a base editors

Kempton, H. R., Love, K. S., Guo, L. Y., & Qi, L. S. (2022) · Nature Chemical Biology · doi:10.1038/s41589-022-01034-2 · Cited by 30

Bacteriophages in nature: recent advances in research tools and diverse environmental and biotechnological applications

Bisen, M., Kharga, K., Mehta, S., Jabi, N., & Kumar, L. (2024) · Environmental Science and Pollution Research · doi:10.1007/s11356-024-32535-3 · Cited by 31

Expanding plant genome editing scope and profiles with CRISPR‐FrCas9 systems targeting palindromic TA sites

He, Y., Han, Y., Ma, Y., Liu, S., Fan, T., Liang, Y., Tang, X., Zheng, X., Wu, Y., Zhang, T., et al. (2024) · Plant Biotechnology Journal · doi:10.1111/pbi.14363 · Cited by 29

Multiplex CRISPR-Cas Genome Editing: Next-Generation Microbial Strain Engineering

Lim, S. R., & Lee, S. J. (2024) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.4c01650 · Cited by 28

High-fidelity PAMless base editing of hematopoietic stem cells to treat chronic granulomatous disease

Bzhilyanskaya, V., Ma, L., Liu, S., Fox, L. R., Whittaker, M. N., Meis, R. J., Choi, U., Lawson, A., Ma, M., Theobald, N., et al. (2024) · Science Translational Medicine · doi:10.1126/scitranslmed.adj6779 · Cited by 27

Repairing a deleterious domestication variant in a floral regulator gene of tomato by base editing

Glaus, A. N., Brechet, M., Swinnen, G., Lebeigle, L., Iwaszkiewicz, J., Ambrosini, G., Julca, I., Zhang, J., Roberts, R., Iseli, C., et al. (2025) · Nature Genetics · doi:10.1038/s41588-024-02026-9 · Cited by 28

Dual SORT LNPs for multi-organ base editing

Kim, M., Song, E. S., Chen, J. C., Chatterjee, S., Sun, Y., Lee, S. M., Wu, S., Patel, P., Tian, Z., Kantor, A., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-025-02675-z · Cited by 39

Efficient mitochondrial A-to-G base editors for the generation of mitochondrial disease models

Chen, L., Hong, M., Luan, C., Yuan, M., Wang, Y., Guo, X., Fang, Y., Huang, H., Dong, X., Gao, H., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-025-02685-x · Cited by 28

In vivo base editing gene therapy for heterozygous familial hypercholesterolemia: a phase 1 trial

Wan, P., Tang, S., Yang, T., Lu, Y., Long, M., Xiao, L., Jiang, Y., Liao, J., Ma, X., Liu, Y., et al. (2025) · Nature Medicine · doi:10.1016/j.atherosclerosis.2025.119507 · Cited by 41

Versatile and efficient in vivo genome editing with compact Streptococcus pasteurianus Cas9

Liu, Z., Chen, S., Xie, W., Song, Y., Li, J., Lai, L., & Li, Z. (2021) · Molecular Therapy · doi:10.1016/j.ymthe.2021.06.013 · Cited by 29

Genome editing with type II‐C CRISPR‐Cas9 systems from Neisseria meningitidis in rice

Xu, R., Qin, R., Xie, H., Li, J., Liu, X., Zhu, M., Sun, Y., Yu, Y., Lu, P., & Wei, P. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13716 · Cited by 29

Epitope prime editing shields hematopoietic cells from CD123 immunotherapy for acute myeloid leukemia

Ji, R.-J., Cao, G.-H., Zhao, W., Wang, M.-Y., Gao, P., Zhang, Y.-Z., Wang, X.-B., Qiu, H.-Y., Chen, D.-D., Tong, X.-H., et al. (2024) · Cell stem cell · doi:10.1016/j.stem.2024.09.003 · Cited by 30

Dimerization of the deaminase domain and locking interactions with Cas9 boost base editing efficiency in ABE8e

Arantes, P. R., Chen, X., Sinha, S., Saha, A., Patel, A. C., Sample, M., Nierzwicki, Ł., Lapinaitė, A., & Palermo, G. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae1066 · Cited by 27

CRISPR-based therapeutic genome editing for inherited blood disorders

Levesque, S., & Bauer, D. E. (2025) · Nature Reviews Drug Discovery · doi:10.1038/s41573-025-01236-y · Cited by 31

Genome editing approaches to β-hemoglobinopathies

Brusson, M., & Miccio, A. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2021.01.025 · Cited by 29

A base editing strategy using mRNA-LNPs for in vivo correction of the most frequent phenylketonuria variant

Brooks, D. L., Whittaker, M. N., Said, H., Dwivedi, G., Qü, P., Musunuru, K., Ahrens‐Nicklas, R. C., Alameh, M., & Wang, X. (2023) · Human Genetics and Genomics Advances · doi:10.1016/j.xhgg.2023.100253 · Cited by 33

Expanded palette of RNA base editors for comprehensive RBP-RNA interactome studies

Medina-Muñoz, H. C., Kofman, E. R., Jagannatha, P., Boyle, E. A., Yu, T., Jones, K. L., Mueller, J. R., Lykins, G. D., Doudna, A. T., Park, S. S., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-45009-4 · Cited by 27

Continuous evolution of base editors with expanded target compatibility and improved activity

Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, W.-H., Zheng, C., Newby, G. A., Wilson, C., Bhaumik, M., Shubina-Oleinik, O., Holt, J. R., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0193-0 · Cited by 347

Directed evolution of adenine base editors with increased activity and therapeutic application

Gaudelli, N. M., Lam, D. K., Rees, H. A., Solá-Esteves, N. M., Barrera, L. A., Born, D. A., Edwards, A., Gehrke, J. M., Lee, S., Liquori, A., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0491-6 · Cited by 533

Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity

Neugebauer, M. E., Hsu, A., Arbab, M., Krasnow, N. A., McElroy, A. N., Pandey, S., Doman, J. L., Huang, T. P., Raguram, A., Banskota, S., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01533-6 · Cited by 282

PhieABEs: a PAM‐less/free high‐efficiency adenine base editor toolbox with wide target scope in plants

Tan, J., Zeng, D., Zhao, Y., Wang, Y., Liu, T., Li, S., Xue, Y., Luo, Y., Xie, X., Chen, L., et al. (2022) · Plant Biotechnology Journal · doi:10.1111/pbi.13774 · Cited by 80

Phage-assisted evolution of highly active cytosine base editors with enhanced selectivity and minimal sequence context preference

Zhang, E., Neugebauer, M. E., Krasnow, N. A., & Liu, D. R. (2024) · Nature Communications · doi:10.1038/s41467-024-45969-7 · Cited by 68