Cited by 386RNA modifications and cancerRNA regulation and diseaseEpigenetics and DNA Methylation

Absolute quantification of single-base m6A methylation in the mammalian transcriptome using GLORI

Liu, Cong, Sun, Hanxiao, Yi, Yunpeng, Shen, Weiguo, Li, Kai, Xiao, Ye, Li, Fei, Li, Yuchen +7 more·2023·Nature Biotechnology

Abstract

N 6 -methyladenosine (m 6 A) is the most abundant RNA modification in mammalian cells and the best-studied epitranscriptomic mark. Despite the development of various tools to map m 6 A, a transcriptome-wide method that enables absolute quantification of m 6 A at single-base resolution is lacking. Here we use glyoxal and nitrite-mediated deamination of unmethylated adenosines (GLORI) to develop an absolute m 6 A quantification method that is conceptually similar to bisulfite-sequencing-based quantification of DNA 5-methylcytosine. We apply GLORI to quantify the m 6 A methylomes of mouse and human cells and reveal clustered m 6 A modifications with differential distribution and stoichiometry. In addition, we characterize m 6 A dynamics under stress and examine the quantitative landscape of m 6 A modification in gene expression regulation. GLORI is an unbiased, convenient method for the absolute quantification of the m 6 A methylome.

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Crossref · 10.1038/s41587-022-01487-9

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Authors
Liu, C., Sun, H., Yi, Y., Shen, W., Li, K., Xiao, Y., Li, F., Li, Y., Hou, Y., Lu, B., Liu, W., Meng, H., Peng, J., Yi, C., & Wang, J.
Affiliations
  • Peking University (CN)
  • Center for Life Sciences (CN)
Corresponding author
  • Yi, Chengqi
  • Wang, Jing
Venue
Nature Biotechnology
Published
2022-10-27
Volume
41
Issue
3
Pages
355-366
ISSN
  • 0733-222X (ISSN-L)
  • 1087-0156
  • 1546-1696
OpenAlex
W4307500724
Cited by
386
References
67
Citations by year
2026: 100 · 2025: 125 · 2024: 103 · 2023: 53 · 2022: 4 · 2020: 1
FWCI
23.26
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Natural Science Foundation of China (22077006, 91940304)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords
N6-methyladenosine, m6A RNA methylation, epitranscriptomics, absolute quantification, single-base resolution, m6A methylome, stress response, gene expression regulation, mouse cells, human cells
MeSH terms
Animals, DNA, Gene Expression Regulation, Humans, Mammals, Methylation, DNA Methylation, Transcriptome

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