The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
Hydrogel-based biocontainment of bacteria for continuous sensing and computation
Open accessAbstract
Genetically modified microorganisms (GMMs) can enable a wide range of important applications including environmental sensing and responsive engineered living materials. However, containment of GMMs to prevent environmental escape and satisfy regulatory requirements is a bottleneck for real-world use. While current biochemical strategies restrict unwanted growth of GMMs in the environment, there is a need for deployable physical containment technologies to achieve redundant, multi-layered and robust containment. We developed a hydrogel-based encapsulation system that incorporates a biocompatible multilayer tough shell and an alginate-based core. This deployable physical containment strategy (DEPCOS) allows no detectable GMM escape, bacteria to be protected against environmental insults including antibiotics and low pH, controllable lifespan and easy retrieval of genomically recoded bacteria. To highlight the versatility of DEPCOS, we demonstrated that robustly encapsulated cells can execute useful functions, including performing cell–cell communication with other encapsulated bacteria and sensing heavy metals in water samples from the Charles River. A dual-layer encapsulation approach provides physical containment of genetically modified bacteria (especially when combined with chemical containment) while also protecting them from environmental stressors and maintaining their sensing functions.
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Cites
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Self-citationHydrogels for biomedical applications
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Cited by
Materials design by synthetic biology
Tang, T.-C., An, B., Huang, Y., Vasikaran, S., Wang, Y., Jiang, X., Lu, T. K., & Zhong, C. (2020) · Nature Reviews Materials · doi:10.1038/s41578-020-00265-w · Cited by 386
Self-citationEngineered microbial consortia: strategies and applications
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Design and regulation of engineered bacteria for environmental release
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Engineering Microbial Consortia as Living Materials: Advances and Prospectives
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Metadata
- Authors
- Tang, T.-C., Tham, E., Liu, X., Yehl, K., Rovner, A. J., Yuk, H., de la Fuente-Nunez, C., Isaacs, F. J., Zhao, X., & Lu, T. K.
- Venue
- Nature Chemical Biology
- Published
- 2021-04-05
- Volume
- 17
- Issue
- 6
- Pages
- 724-731
- ISSN
- 1552-4450 (ISSN-L)
- 1552-4469
- OpenAlex
- W3149090762
- Cited by
- 258
- References
- 64
- FWCI
- 10.25
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Tang, T.-C., Tham, E., Liu, X., Yehl, K., Rovner, A. J., Yuk, H., de la Fuente-Nunez, C., Isaacs, F. J., Zhao, X., & Lu, T. K. (2021). Hydrogel-based biocontainment of bacteria for continuous sensing and computation. Nature Chemical Biology. https://doi.org/10.1038/s41589-021-00779-6
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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