Cited by 403Systemic Lupus Erythematosus ResearchRenal Diseases and GlomerulopathiesSystemic Sclerosis and Related Diseases

The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.

Tubular cell and keratinocyte single-cell transcriptomics applied to lupus nephritis reveal type I IFN and fibrosis relevant pathways

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Abstract

The molecular and cellular processes that lead to renal damage and to the heterogeneity of lupus nephritis (LN) are not well understood. We applied single-cell RNA sequencing (scRNA-seq) to renal biopsies from patients with LN and evaluated skin biopsies as a potential source of diagnostic and prognostic markers of renal disease. Type I interferon (IFN)-response signatures in tubular cells and keratinocytes distinguished patients with LN from healthy control subjects. Moreover, a high IFN-response signature and fibrotic signature in tubular cells were each associated with failure to respond to treatment. Analysis of tubular cells from patients with proliferative, membranous and mixed LN indicated pathways relevant to inflammation and fibrosis, which offer insight into their histologic differences. In summary, we applied scRNA-seq to LN to deconstruct its heterogeneity and identify novel targets for personalized approaches to therapy.

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Metadata

Authors
the Accelerating Medicines Partnership Rheumatoid Arthritis and Systemic Lupus Erythematosus (AMP RA/SLE) Consortium, Der, E., Suryawanshi, H., Morozov, P., Kustagi, M., Goilav, B., Ranabothu, S., Izmirly, P., Clancy, R., Belmont, H. M., Koenigsberg, M., Mokrzycki, M., Rominieki, H., Graham, J. A., Rocca, J. P., Bornkamp, N., Jordan, N., Schulte, E., Wu, M., Pullman, J., Slowikowski, K., Raychaudhuri, S., Guthridge, J., James, J., Buyon, J., Tuschl, T., & Putterman, C.
Affiliations
  • Albert Einstein College of Medicine (US)
  • Rockefeller University (US)
  • Arkansas Children's Hospital (US)
  • New York University (US)
  • Montefiore Medical Center (US)
  • Broad Institute (US)
  • Oklahoma Medical Research Foundation (US)
Corresponding author
  • Buyon, Jill
  • Tuschl, Thomas
  • Putterman, Chaim
Venue
Nature Immunology
Published
2019-05-20
Volume
20
Issue
7
Pages
915-927
ISSN
  • 1529-2908 (ISSN-L)
  • 1529-2916
OpenAlex
W2944872709
Cited by
403
References
52
Citations by year
2026: 30 · 2025: 35 · 2024: 61 · 2023: 44 · 2022: 86 · 2021: 56 · 2020: 64 · 2019: 25
FWCI
44.01
Type
journal article
Language
en
Open access
Locations
  • Nature Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • National Institutes of Health (UL1TR003167, UL1TR000371)
  • National Human Genome Research Institute (U01HG009379, T32HG002295)
  • National Institute of Allergy and Infectious Diseases
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (UH2AR067679, UH2AR067677, UH2AR067685, UH2AR067681, UH2AR067689, F31AR070582, UH2AR067676, UH2AR067694, UH2AR067691, UH2AR067690, UH2AR067688, UM2AR067678)
  • National Center for Advancing Translational Sciences
Topics
  • Health Sciences › Medicine › Rheumatology › Systemic Lupus Erythematosus Research
  • Health Sciences › Medicine › Nephrology › Renal Diseases and Glomerulopathies
  • Health Sciences › Medicine › Rheumatology › Systemic Sclerosis and Related Diseases
Keywords
lupus nephritis, type I interferon response, renal tubular epithelial cells, keratinocytes, renal fibrosis, treatment non-response, skin biopsy, disease heterogeneity, membranous lupus nephritis, proliferative lupus nephritis, single-cell transcriptomics, personalized treatment
MeSH terms
Biopsy, Fibrosis, Humans, Interferon Type I, Kidney Tubules, Lupus Nephritis, Protein Binding, Skin, Signal Transduction, Keratinocytes, Extracellular Matrix Proteins, Cell Lineage, Computational Biology, Gene Expression Profiling, Single-Cell Analysis, Transcriptome

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