Cited by 263Insect Utilization and EffectsAnimal Nutrition and PhysiologyGut microbiota and health

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Insect-based diet, a promising nutritional source, modulates gut microbiota composition and SCFAs production in laying hens.

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Borrelli, Luca, Coretti, Lorena, Dipineto, Ludovico, Bovera, Fulvia, Menna, Francesca, Chiariotti, Lorenzo, Nizza, Antonio, Lembo, Francesca, Fioretti, Alessandro·2017·Scientific Reports

Abstract

Insects could be potential nutritional sources both for humans and animals. Among these, Hermetia illucens, with good amount of chitin and proteins, represents a suitable diet replacement for laying hens. Little is known about insect diet effects on the microbial ecology of the gastrointestinal tract and bacterial metabolites production. In this study we investigated the effect of H. illucens larvae meal administration on cecal microbiota and short chain fatty acids (SCFAs) production in laying hens. 16S rDNA sequencing showed strong differences between cecal microbiota of soybean (SD) and insect diet (ID) groups both in type and relative abundance (unweighted and weighted beta diversity) of microbial species. In particular, Bacteroides plebeius, Elusimicrobium minutum, Alkaliphilus transvaalensis, Christensenella minuta, Vallitalea guaymasensis and Flavonifractor plautii represented the principal contributors of changes in gut microbiota composition of ID group (FDR p-values < 0.05). Of these, F. plautii, C. minuta and A. transvaalensis have the potential to degrade the chitin’s insect meal and correlated with the observed high levels of gut SCFAs produced in ID group. These microorganisms may thus connect the chitin degradation with high SCFAs production. Our results suggest H. illucens as a potential prebiotic by well feeding gut microbiota.

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Crossref · 10.1038/s41598-017-16560-6

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Figures

Figure 1 Cecal microbiota structure after insect-based diet administration. (A) Diversity within SD and ID microbial communities measured by number of observed species and Shannon index. Data are mean ± SEM. Significant differences are indicated by *p < 0.05 and **p < 0.01 (two-sided Student’s two-sample t-test). (B, C) PCoA plots based, respectively, on unweighted and weighted UniFrac distances of SD and ID gut microbial communities (5,234 sequences/sample).
Figure 1 Cecal microbiota structure after insect-based diet administration. (A) Diversity within SD and ID microbial communities measured by number of observed species and Shannon index. Data are mean ± SEM. Significant differences are indicated by *p < 0.05 and **p < 0.01 (two-sided Student’s two-sample t-test). (B, C) PCoA plots based, respectively, on unweighted and weighted UniFrac distances of SD and ID gut microbial communities (5,234 sequences/sample).CC BY · PubMed Central
Figure 2 (A) Phylogenetic composition at phylum level of SD and ID microbial communities. (B,C) Relative abundances (%) of bacterial phyla and genera, respectively, found to be significantly different between SD and ID groups (p < 0.05; mean ± SEM). *p < 0.05, **p < 0.01 after FDR correction.
Figure 2 (A) Phylogenetic composition at phylum level of SD and ID microbial communities. (B,C) Relative abundances (%) of bacterial phyla and genera, respectively, found to be significantly different between SD and ID groups (p < 0.05; mean ± SEM). *p < 0.05, **p < 0.01 after FDR correction.CC BY · PubMed Central
Figure 3 Stacked bar chart showing the count of metagenomic contributors for beta-N-acetylhexosaminidase (K01207) and N-acetylglucosamine-6-phosphate deacetylase (K01443) in SD and ID samples.
Figure 3 Stacked bar chart showing the count of metagenomic contributors for beta-N-acetylhexosaminidase (K01207) and N-acetylglucosamine-6-phosphate deacetylase (K01443) in SD and ID samples.CC BY · PubMed Central
Figure 4 SCFAs in cecal samples of ID and SD groups. (A) Stacked bar chart shows mean values of cecal SCFAs in SD and ID groups. (B) Heatmap shows the Pearson’s correlation between SCFAs concentration (y-axis) and key genera at species level identified by SPINGO (bottom, x-axis). Blue and red colors designate, respectively, the positive and negative correlations. *p < 0.05, **p < 0.01, ***p < 0.001.
Figure 4 SCFAs in cecal samples of ID and SD groups. (A) Stacked bar chart shows mean values of cecal SCFAs in SD and ID groups. (B) Heatmap shows the Pearson’s correlation between SCFAs concentration (y-axis) and key genera at species level identified by SPINGO (bottom, x-axis). Blue and red colors designate, respectively, the positive and negative correlations. *p < 0.05, **p < 0.01, ***p < 0.001.CC BY · PubMed Central

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Metadata

Authors
Borrelli, L., Coretti, L., Dipineto, L., Bovera, F., Menna, F., Chiariotti, L., Nizza, A., Lembo, F., & Fioretti, A.
Affiliations
  • University of Naples Federico II (IT)
  • Institute for Experimental Endocrinology and Oncology (IT)
Corresponding author
Borrelli, Luca
Venue
Scientific Reports
Published
2017-11-24
Volume
7
Issue
1
Pages
16269-16269
ISSN
2045-2322 (ISSN-L)
OpenAlex
W2768218657
Cited by
263
References
60
Citations by year
2026: 18 · 2025: 37 · 2024: 33 · 2023: 39 · 2022: 36 · 2021: 31 · 2020: 37 · 2019: 25 · 2018: 7
FWCI
17.53
Type
journal article
Language
en
Open access
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Università degli Studi di Firenze
Topics
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect Utilization and Effects
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
Hermetia illucens, insect meal, laying hens, gut microbiota, short-chain fatty acids, cecal microbiota, chitin degradation, prebiotics, soybean diet, Flavonifractor plautii, Christensenella minuta, Bacteroides plebeius, poultry nutrition
MeSH terms
Gastrointestinal Microbiome, Animals, Animal Feed, Chickens, Diet, Fatty Acids, Volatile, Female, Insecta

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