Eradication of Candida albicans persister cell biofilm by the membranotropic peptide gH625
Open accessAbstract
Biofilm formation poses an important clinical trouble due to resistance to antimicrobial agents; therefore, there is an urgent demand for new antibiofilm strategies that focus on the use of alternative compounds also in combination with conventional drugs. Drug-tolerant persisters are present in Candida albicans biofilms and are detected following treatment with high doses of amphotericin B. In this study, persisters were found in biofilms treated with amphotericin B of two clinical isolate strains, and were capable to form a new biofilm in situ. We investigated the possibility of eradicating persister-derived biofilms from these two Candida albicans strains, using the peptide gH625 analogue (gH625-M). Confocal microscopy studies allowed us to characterize the persister-derived biofilm and understand the mechanism of interaction of gH625-M with the biofilm. These findings confirm that persisters may be responsible for Candida biofilm survival, and prove that gH625-M was very effective in eradicating persister-derived biofilms both alone and in combination with conventional antifungals, mainly strengthening the antibiofilm activity of fluconazole and 5-flucytosine. Our strategy advances our insights into the development of effective antibiofilm therapeutic approaches.
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gH625-M successfully eradicated persister-derived biofilms of C. albicans in vitro, both alone and in combination with fluconazole and 5-fucytosine.
“gH625-M, a peptide analogue of gH625 monomer, has been found to successfully eradicate persister-derived biofilms of C. albicans in vitro, both alone and in combination with fluconazole and 5-fucytosine [53].”
From Antifungal persistence: Clinical relevance and mechanisms (2025) · cites this paper as [53]
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- Authors
- Galdiero, E., de Alteriis, E., De Natale, A., D’Alterio, A., Siciliano, A., Guida, M., Lombardi, L., Falanga, A., & Galdiero, S.
- Affiliations
- University of Naples Federico II (IT)
- Corresponding author
- Galdiero, Stefania
- ORCID
- Venue
- Scientific Reports
- Published
- 2020-04-01
- Volume
- 10
- Issue
- 1
- Pages
- 5780-5780
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC7113253
- OpenAlex
- W3014374127
- Cited by
- 65
- References
- 59
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 7 · 2023: 10 · 2022: 12 · 2021: 15 · 2020: 3 · 2019: 1
- FWCI
- 1.31
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research Portal (Queen's University Belfast) (submittedVersion, cc-by)
- Funders
- Regione Campania (B61G18000470007, Project N. B61G18000470007, FESR 2014 2020)
- European Regional Development Fund (FESR 2014-2020, 2014-2020’)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Keywords
- Candida albicans, biofilm, drug-tolerant persisters, amphotericin B, antifungal resistance, fluconazole, 5-fluorocytosine, antibiofilm therapy
- MeSH terms
- Amino Acid Sequence, Amphotericin B, Antifungal Agents, Candida albicans, Candidiasis, Humans, Drug Design, Biofilms, Antimicrobial Cationic Peptides, Drug Resistance, Fungal
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