Trichosporon asahii and Trichosporon inkin biofilms produce antifungal-tolerant persister cells

Cordeiro, Rossana de Aguiar; Aguiar, Ana Luiza Ribeiro; da Silva, Bruno Nascimento; Pereira, Lívia Maria Galdino; Portela, Fernando Victor Monteiro; de Camargo, Zoilo Pires; de Lima-Neto, Reginaldo Gonçalves; Castelo-Branco, Débora de Souza Collares Maia; Rocha, Marcos Fábio Gadelha; Sidrim, José Júlio Costa

doi:10.3389/fcimb.2021.645812

Cited by 34Antifungal resistance and susceptibilityFungal and yeast genetics researchBacterial biofilms and quorum sensing

Trichosporon asahii and Trichosporon inkin biofilms produce antifungal-tolerant persister cells

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Cordeiro, Rossana de Aguiar, Aguiar, Ana Luiza Ribeiro, da Silva, Bruno Nascimento, Pereira, Lívia Maria Galdino, Portela, Fernando Victor Monteiro, de Camargo, Zoilo Pires, de Lima-Neto, Reginaldo Gonçalves, Castelo-Branco, Débora de Souza Collares Maia +2 more·2021·Frontiers in Cellular and Infection Microbiology

DOI 10.3389/fcimb.2021.645812PMC PMC8100310

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Abstract

Persister cells are metabolically inactive dormant cells that lie within microbial biofilms. They are phenotypic variants highly tolerant to antimicrobials and, therefore, associated with recalcitrant infections. In the present study, we investigated if Trichosporon asahii and T. inkin are able to produce persister cells.Trichosporon spp. are ubiquitous fungi, commonly found as commensals of the human skin and gut microbiota, and have been increasingly reported as agents of fungemia in immunocompromised patients. Biofilms derived from clinical strains of T asahii(n=5) and T. inkin(n=7) were formed in flat-bottomed microtiter plates and incubated at 35°C for 48 h, treated with 100 μg/ml amphotericin B (AMB) and incubated at 35°C for additional 24 h. Biofilms were scraped from the wells and persister cells were assayed for susceptibility to AMB. Additionally, we investigated if these persister cells were able to generate new biofilms and studied their ultrastructure and AMB susceptibility. Persister cells were detected in both T asahii and T. inkin biofilms and showed tolerance to high doses of AMB (up to 256 times higher than the minimum inhibitory concentration). Persister cells were able to generate biofilms, however they presented reduced biomass and metabolic activity, and reduced tolerance to AMB, in comparison to biofilm growth control. The present study describes the occurrence of persister cells in Trichosporon spp. and suggests their role in the reduced AMB susceptibility of T.asahii and T. inkin biofilms.

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Amphotericin B has been widely studied and shown to induce antifungal persistence in Candida spp. biofilms and Trichosporon spp.
“Amphotericin B which belongs to polyenes class, due to its potent fungicidal activity and the rarity of resistance [35–37], has been widely studied and shown to induce antifungal persistence in Candida spp. biofilms and Trichosporon spp.”
From Antifungal persistence: Clinical relevance and mechanisms (2025) · cites this paper as [37]
The Discussion reports amphotericin B-tolerant persister cells in Candida biofilms and surviving cells in amphotericin B-treated Trichosporon biofilms. It does not show that the drug induces those cells, rather than revealing cells that survive exposure. The Introduction also says few studies had addressed antifungal-resistance mechanisms in Trichosporon, so 'widely studied' is not supported for that genus. Page numbers are unavailable in our copy. (LLM, conf=0.84)
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Authors: Cordeiro, R. D. A., Aguiar, A. L. R., da Silva, B. N., Pereira, L. M. G., Portela, F. V. M., de Camargo, Z. P., de Lima-Neto, R. G., Castelo-Branco, D. D. S. C. M., Rocha, M. F. G., & Sidrim, J. J. C.
Affiliations: Universidade Federal do Ceará (BR)
Universidade Estadual do Ceará (BR)
Corresponding author: Cordeiro, Rossana de Aguiar
ORCID: Cordeiro, Rossana de Aguiar
Aguiar, Ana Luiza Ribeiro
Portela, Fernando Victor Monteiro
Rocha, Marcos Fábio Gadelha
Sidrim, José Júlio Costa
Venue: Frontiers in Cellular and Infection Microbiology
Publisher: Frontiers Media SA
Published: 2021-04-22
Volume: 11
Pages: 645812-645812
ISSN: 2235-2988 (ISSN-L)
DOI: 10.3389/fcimb.2021.645812
PMC: PMC8100310
OpenAlex: W3160946572
Cited by: 34
References: 44
Citations by year: 2026: 5 · 2025: 10 · 2024: 4 · 2023: 3 · 2022: 11 · 2021: 1
FWCI: 1.50
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (vor)
Locations: Frontiers in Cellular and Infection Microbiology (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders: Conselho Nacional de Desenvolvimento Científico e Tecnológico (430193/2018-1)
Topics: Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Keywords: Trichosporon asahii, Trichosporon inkin, persister cells, biofilm, antifungal tolerance, amphotericin B, fungemia, recalcitrant infection, biofilm ultrastructure, clinical isolates
MeSH terms: Antifungal Agents, Basidiomycota, Humans, Microbial Sensitivity Tests, Trichosporon, Biofilms

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Trichosporon asahii and Trichosporon inkin biofilms produce antifungal-tolerant persister cells

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