Expression of green fluorescent protein defines a specific population of lamina II excitatory interneurons in the GRP::eGFP mouse
Open accessAbstract
Dorsal horn excitatory interneurons that express gastrin-releasing peptide (GRP) are part of the circuit for pruritogen-evoked itch. They have been extensively studied in a transgenic line in which enhanced green fluorescent protein (eGFP) is expressed under control of the Grp gene. The GRP-eGFP cells are separate from several other neurochemically-defined excitatory interneuron populations, and correspond to a class previously defined as transient central cells. However, mRNA for GRP is widely distributed among excitatory interneurons in superficial dorsal horn. Here we show that although Grp mRNA is present in several transcriptomically-defined populations, eGFP is restricted to a discrete subset of cells in the GRP::eGFP mouse, some of which express the neuromedin receptor 2 and likely belong to a cluster defined as Glut8. We show that these cells receive much of their excitatory synaptic input from MrgA3/MrgD-expressing nociceptive/pruritoceptive afferents and C-low threshold mechanoreceptors. Although the cells were not innervated by pruritoceptors expressing brain natriuretic peptide (BNP) most of them contained mRNA for NPR1, the receptor for BNP. In contrast, these cells received only ~ 10% of their excitatory input from other interneurons. These findings demonstrate that the GRP-eGFP cells constitute a discrete population of excitatory interneurons with a characteristic pattern of synaptic input.
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The Reln dorsal excitatory family comprised Excit-8, Excit-9, Excit-10, and Excit-11 and expressed markers indicative of a role in chemical itch sensation.
“The Reln dorsal, excitatory family was comprised of Excit-8, Excit-9, Excit-10, and Excit-11 and expressed markers indicative of a role in chemical itch sensation58–62.”
From A harmonized atlas of mouse spinal cord cell types and their spatial organization (2021) · cites this paper as [58]
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A harmonized atlas of mouse spinal cord cell types and their spatial organization
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- Authors
- Bell, A. M., Gutierrez-Mecinas, M., Stevenson, A., Casas-Benito, A., Wildner, H., West, S. J., Watanabe, M., & Todd, A. J.
- Affiliations
- University of Glasgow (GB)
- University of Zurich (CH)
- John Radcliffe Hospital (GB)
- Hokkaido University (JP)
- Corresponding author
- Bell, Andrew M.
- Todd, Andrew J.
- Venue
- Scientific Reports
- Published
- 2020-08-06
- Volume
- 10
- Issue
- 1
- Pages
- 13176-13176
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC7411045
- OpenAlex
- W3047547106
- Cited by
- 25
- References
- 60
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 1 · 2023: 4 · 2022: 5 · 2021: 7 · 2020: 2
- FWCI
- 2.71
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Zurich Open Repository and Archive (University of Zurich) (acceptedVersion, cc-by)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (acceptedVersion, cc-by-sa)
- Repository for Publications and Research Data (ETH Zurich) (submittedVersion, cc-by)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion, cc-by)
- ETH Zürich Research Collection (cc-by)
- Zurich Open Repository and Archive (University of Zurich)
- Funders
- Wellcome Trust (102645)
- Medical Research Council (MR/S002987/1)
- Topics
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- gastrin-releasing peptide, lamina II, GLUT8, brain natriuretic peptide, NPR1, pruritus, synaptic input
- MeSH terms
- Animals, Interneurons, Substantia Gelatinosa, Synapses, Gene Expression, Green Fluorescent Proteins, Mice
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