Cited by 167Sesquiterpenes and Asteraceae StudiesNatural Compounds in Disease TreatmentPhytochemistry and Biological Activities

Effect of two active compounds obtained from the essential oil of Cordia verbenacea on the acute inflammatory responses elicited by LPS in the rat paw

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Medeiros, R, Passos, G F, Vitor, C E, Koepp, J, Mazzuco, T L, Pianowski, L F, Campos, M M, Calixto, J B·2007·British Journal of Pharmacology

Abstract

BACKGROUND AND PURPOSE: alpha-Humulene and trans-caryophyllene are sesquiterpene compounds identified in the essential oil of Cordia verbenacea which display topical and systemic anti-inflammatory effects in different experimental models. However, the molecular mechanisms through which they exert their anti-inflammatory activity still remain unclear. Here, we evaluate the effects of alpha-humulene and trans-caryophyllene on the acute inflammatory responses elicited by LPS. EXPERIMENTAL APPROACH: The biological activities of alpha-humulene and trans-caryophyllene were investigated in a model of acute inflammation in rat paw, induced by LPS and characterized by paw oedema, neutrophil recruitment, cytokine production, activation of MAP kinases and NF-kappaB and up-regulated expression of kinin B(1) receptors. KEY RESULTS: Treatment with either alpha-humulene or trans-caryophyllene effectively reduced neutrophil migration and activation of NF-kappaB induced by LPS in the rat paw. However, only alpha-humulene significantly reduced the increase in TNF-alpha and IL-1beta levels, paw oedema and the up-regulation of B(1) receptors following treatment with LPS. Both compounds failed to interfere with the activation of the MAP kinases, ERK, p38 and JNK. CONCLUSIONS AND IMPLICATIONS: Both alpha-humulene and trans-caryophyllene inhibit the LPS-induced NF-kappaB activation and neutrophil migration, although only alpha-humulene had the ability to prevent the production of pro-inflammatory cytokines TNF-alpha and IL-1beta and the in vivo up-regulation of kinin B(1) receptors. These data provide additional molecular and functional insights into the beneficial effects of the sesquiterpenes alpha-humulene and trans-caryophyllene isolated from the essential oil of Cordia verbenacea as agents for the management of inflammatory diseases.

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Crossref · 10.1038/sj.bjp.0707270

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  • α-Humulene from Cordia verbenacea reduces the increase of TNF-α and IL-1β levels in rat paw after LPS treatment or in carrageenan-injected rats.

    “Some authors showed that from the essential oil of Cordia verbenacea, only α-humelene was able to reduce significantly the increase of TNF-α and IL-1β levels in the subcutaneous tissue of the rat paw after LPS treatment [113] or in carrageenan-injected rats”
  • α-Humulene and trans-caryophyllene inhibit LPS-induced NF-kB activation and neutrophil migration in the rat paw but are unable to inhibit LPS-induced MAP kinase activation.

    “α-Humulene and trans-caryophyllene, the main constituents of Cordia verbenacea inhibited the LPS-induced NF-kB activation and neutrophil migration in the rat paw, however unable to inhibit LPS-induced MAP kinase activation [113].”

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Authors
Medeiros, R., Passos, G. F., Vitor, C. E., Koepp, J., Mazzuco, T. L., Pianowski, L. F., Campos, M. M., & Calixto, J. B.
Affiliations
  • Universidade Federal de Santa Catarina (BR)
  • Pontifícia Universidade Católica do Rio Grande do Sul (BR)
Venue
British Journal of Pharmacology
Publisher
Wiley
Published
2007-07
Volume
151
Issue
5
Pages
618-627
ISSN
  • 0007-1188 (ISSN-L)
  • 1476-5381
OpenAlex
W1984871487
Cited by
167
References
46
Citations by year
2026: 3 · 2025: 8 · 2024: 7 · 2023: 11 · 2022: 10 · 2021: 12 · 2020: 12 · 2019: 11 · 2018: 11 · 2017: 6 · 2016: 9 · 2015: 16 · 2014: 8 · 2013: 6 · 2012: 6
FWCI
2.23
Type
journal article
Language
en
Open access
Locations
  • British Journal of Pharmacology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
  • Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
Keywords
Cordia verbenacea, α-humulene, β-caryophyllene, sesquiterpenes, essential oils, anti-inflammatory activity, acute inflammation, lipopolysaccharide, paw edema, neutrophil migration, NF-κB, mitogen-activated protein kinase, TNF-α, IL-1β, kinin B1 receptor, inflammatory cytokines
MeSH terms
Polycyclic Sesquiterpenes, Animals, Anti-Inflammatory Agents, Non-Steroidal, Cell Nucleus, Cytosol, Edema, Foot, Lipopolysaccharides, Male, Peroxidase, Neutrophils, Oils, Volatile, Sesquiterpenes, Tumor Necrosis Factor-alpha, Blotting, Western, Up-Regulation, NF-kappa B, Rats, Wistar, Reverse Transcriptase Polymerase Chain Reaction, Neutrophil Infiltration, Mitogen-Activated Protein Kinases, Electrophoretic Mobility Shift Assay, Cordia, Receptor, Bradykinin B1, Rats, Interleukin-1beta

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