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Activation of the protein kinase B pathway by the HPV-16 E7 oncoprotein occurs through a mechanism involving interaction with PP2A

Pim, David, Massimi, Paola, Dilworth, Stephen M, Banks, Lawrence·2005·Oncogene
DOI 10.1038/sj.onc.1208935
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Abstract

Protein kinase B (PKB) or Akt is one of several second messenger kinases that are activated by cell attachment and growth factor signaling, and that transmit signals to the cell nucleus to inhibit apoptosis and thereby increase cell survival during proliferation. Other viral proteins target this pathway by increasing PKB/Akt phosphorylation, and this pathway has been implicated in the transformation of human keratinocytes by HPV E6 and E7, together with activated notch 1. Here, we examine how HPV E7 expression affects the phosphorylation of PKB. We show that HPV-16 E7 increases the level of phosphorylation of PKB in response to serum stimulation, by a mechanism independent of downregulation of PTEN phosphatase, a known inhibitor of the PI3K (PI3 kinase) pathway. The use of specific antibodies shows that some proportion of PKB/Akt that is phosphorylated both on threonine 308 and serine 473 is maintained in the presence of E7 in a PI3 kinase-independent manner, and is activated for phosphorylation of BAD, a known downstream target of PKB/Akt. Use of E7 mutants has ruled out both an inhibition of IGFBP-3, a known E7 target and PKB/Akt modulator, and the interaction of E7 with cellular pocket proteins, as being the mechanism for the PKB/Akt stimulation. PKB binds PP2A and is a known substrate of PP2A. Here, we show that HPV E7 also binds to both the 35 kDa catalytic and 65 kDa structural subunits of PP2A, an interaction that sequesters these subunits and inhibits their interactio…

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Research works printed but not linked: 43

  1. Andjelkovic M, Kakubowicz T, Cron P, Ming XF, Han JW and Hemmings BA . (1996). Proc. Natl. Acad. Sci. USA, 93, 5699–5704. · DOI
  2. Borgatti P, Zauli G, Colamussi M, Gibellini G, Previati M, Cantley L and Capitani S . (1997). Eur. J. Immunol., 27, 2805–2811. · DOI
  3. Brazil D and Hemmings BA . (2001). Trends Biochem. Sci., 26, 657–664. · DOI · Paper page
  4. Brunet A, Bonni A, Zigmond M, Lin M, Juo P, Hu L, Anderson M, Arden K, Blenis J and Greenberg M . (1999). Cell, 96, 857–868. · DOI · Paper page
  5. Crusius K, Auvinen E and Alonso A . (1997). Oncogene, 15, 1437–1444. · DOI · Paper page
  6. Dajee M, Tarutani M, Deng H, Cai T and Khavari PA . (2002). Oncogene, 21, 1527–1538. · DOI
  7. Datta SR, Dudek H, Tao X, Masters S, Fu H, Gotoh Y and Greenberg ME . (1997). Cell, 91, 231–241. · DOI · Paper page
  8. Delcommene M, Tan C, Gray V, Rue L, Woodget J and Dedhar S . (1998). Proc. Natl. Acad. Sci. USA, 95, 11211–11216. · DOI
  9. Dilworth SM and Horner VP . (1993). J. Virol., 67, 2235–2244. · DOI
  10. Frese KK, Lee SS, Thomas DL, Latorre IJ, Weiss RS, Glaunsinger BA and Javier RT . (2003). Oncogene, 22, 710–721. · DOI
  11. Fujisaki H and Hattori S . (2002). Exp. Cell. Res., 280, 255–269. · DOI
  12. Graham FL and van der Eb AJ . (1973). Virology, 52, 456–467. · DOI
  13. Gu Z and Matlashewski G . (1995). J. Virol., 69, 8051–8056. · DOI · Paper page
  14. Hanada M, Feng J and Hemmings BA . (2004). Biochim. Biophys. Acta, 1697, 3–16. · DOI
  15. Harlow E and Lane D . (1988). Antibodies, A Laboratory Manual. Cold Spring Harbor Publications, Cold Spring Harbor, NY.
  16. Hawley-Nelson P, Vousden KH, Hubbert NL, Lowy DR and Schiller JT . (1989). EMBO J., 8, 3905–3910. · DOI · Paper page
  17. He Y, Nakao H, Tan S-L, Polyak SJ, Neddermann P, Vijaysri S, Jacobs BL and Katze MG . (2002). J. Virol., 76, 9207–9217. · DOI
  18. Ishiwatari H, Hayasaka N, Inoue H, Yutsudo M and Hakura A . (1994). J. Med. Virol., 44, 243–249. · DOI
  19. Mannhardt B, Weinzimer SA, Wagner M, Fiedler M, Cohen P, Jansen-Durr P and Zwerschke W . (2000). Mol. Cell. Biol., 20, 6483–6495. · DOI
  20. Massimi P, Pim D and Banks L . (1997). J. Gen. Virol., 78, 2607–2613. · DOI
  21. Matlashewski G, Schneider J, Banks L, Jones N, Murray A and Crawford L . (1987). EMBO J., 6, 1741–1746. · DOI · Paper page
  22. Meili R, Cron P, Hemmings BA and Ballmer-Hofer K . (1998). Oncogene, 16, 903–907. · DOI
  23. Milburn CC, Deak M, Kelly SM, Price NC, Alessi DR and Van Aaltern DM . (2003). Biochem. J., 375, 531–538. · DOI
  24. Münger K, Phelps WC, Bubb V, Howley PM and Schlegel R . (1989). J. Virol., 63, 4417–4423. · DOI · Paper page
  25. Pallas DC, Shahrik LK, Martin BL, Jaspers S, Miller TB, Brautigan DL and Roberts TM . (1990). Cell, 60, 167–176. · DOI
  26. Pallas DC, Weller W, Jaspers S, Miller TB, Lane WS and Roberts TM . (1992). J. Virol., 66, 886–893. · DOI
  27. Pankov R, Cukierman E, Clark K, Matsumoto K, Hahn C, Poulin B and Yamada KM . (2003). J. Biol. Chem., 278, 18671–18681. · DOI
  28. Pim D, Massimi P and Banks L . (1997). Oncogene, 15, 257–264. · DOI
  29. Pouliot N, Saunders NA and Kaur P . (2002). Exp. Dermatol., 11, 387–397. · DOI
  30. Rangarajan A, Syal R, Selvarajah S, Chakrabarti O, Sarin A and Krishna S . (2001). Virology, 286, 23–30. · DOI
  31. Resjo S, Goransson O, Harndahl L, Zolnierowicz S, Manganiello V and Degerman E . (2002). Cell Signal., 14, 231–238. · DOI
  32. Sayama K, Yamasaki K, Hanakawa Y, Shirakata Y, Tokumaru S, Ijuin T and Hashimoto K . (2002). J. Biol. Chem., 277, 40390–40396. · DOI
  33. Smith DB and Johnson KS . (1988). Gene, 67, 31–40. · DOI
  34. Stambolic V, Mak TW and Woodgett JR . (1999). Oncogene, 18, 6094–6103. · DOI
  35. Stambolic V, Suzuki A, de la Pompa JL, Brothers GM, Mirtsos C, Sasaki T, Ruland J, Penninger JM, Siderovski DP and Mak TW . (1998). Cell, 95, 29–39. · DOI · Paper page
  36. Storey A, Pim D, Murray A, Osborn K, Banks L and Crawford LV . (1988). EMBO J., 7, 1815–1820. · DOI
  37. Summers S, Lipfert L and Birnbaum M . (1998). Biochem. Biophys. Res. Commun., 246, 76–81. · DOI
  38. Sun P, Wang XQ, Lopatka K, Bangash S and Paller AS . (2002). J. Invest. Dermatol., 19, 107–117. · DOI
  39. Troussard AA, Mawji NM, Ong C, Mui A, St-Arnaud R and Dedhar S . (2003). J. Biol. Chem., 278, 22374–22378. · DOI
  40. Velling T, Nilsson S, Stefansson A and Johansson S . (2004). EMBO Rep., 5, 901–905. · DOI
  41. Werness B, Levine A and Howley PM . (1990). Science, 248, 76–79. · DOI · Paper page
  42. Westbrook TF, Nguyen DX, Thrash BR and McCance DJ . (2002). Mol. Cell. Biol., 22, 7041–7052. · DOI
  43. Zur Hausen H . (1999). Semin. Cancer Biol., 9, 405–411. · DOI

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Metadata

Authors
Pim, D., Massimi, P., Dilworth, S. M., & Banks, L.
Venue
Oncogene
Publisher
Springer Science and Business Media LLC
Published
2005-07-25
Volume
24
Issue
53
Pages
7830-7838
ISSN
  • 0950-9232
  • 1476-5594
DOI
10.1038/sj.onc.1208935
Cited by
156
References
1
Language
en
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CoChat. (2026). Citation check: Activation of the protein kinase B pathway by the HPV-16 E7 oncoprotein occurs through a mechanism involving interaction with PP2A. https://app.cochat.ai/papers/10.1038/sj.onc.1208935
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Pim, D., Massimi, P., Dilworth, S. M., & Banks, L. (2005). Activation of the protein kinase B pathway by the HPV-16 E7 oncoprotein occurs through a mechanism involving interaction with PP2A. Oncogene. https://doi.org/10.1038/sj.onc.1208935