Hypoxia-inducible miR-210 contributes to preeclampsia via targeting thrombospondin type I domain containing 7A
Open accessAbstract
Preeclampsia, a relatively common pregnancy disorder, is a major contributor to maternal mortality and morbidity worldwide. An elevation in microRNA-210 (miR-210) expression in the placenta has been reported to be associated with preeclampsia. Our bioinformatic analysis showed that thrombospondin type I domain containing 7A (THSD7A) is a predicted target for miR-210. The aim of this study was to determine whether miR-210 is involved in preeclampsia through its targeting of THSD7A in human placental trophoblasts. In preeclamptic placental tissues, THSD7A levels were significantly downregulated and were inversely correlated with the levels of miR-210. THSD7A was validated as a direct target of miR-210 using quantitative real time PCR (qRT-PCR), Western blotting and dual luciferase assays in HTR8/SVneo cells. Transwell insert invasion assays showed that THSD7A mediated the invasion-inhibitory effect of miR-210 in HTR8/SVneo cells. Interestingly, hypoxia markedly increased miR-210 expression while suppressing THSD7A expression in a time-dependent manner in HTR8/SVneo cells. This study provides novel data on the function of THSD7A in human placental cells and extends our knowledge of how miR-210 is involved in the development of the preeclampsia.
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Other targets identified for miR-210 include the steroidogenic enzyme hydroxysteroid (17-ß) dehydrogenase 1, ephrin-A3 and homeobox-A9 which are involved in trophoblast cell migration and vascular remodeling, and thrombospondin type 1 domain containing 7A in the placental vasculature.
“Besides mitochondria, other targets identified for miR-210 include the steroidogenic enzyme hydroxysteroid (17-ß) dehydrogenase 1 (272), ephrin-A3 and homeobox-A9 which are involved in trophoblast cell migration and vascular remodeling (611), and thrombospondin type 1 domain containing 7A in the placental vasculature (351).”
From Placental Origins of Chronic Disease (2016) · cites this paper as [351]
MiR-210 is increased in normal placentas from pregnancies at high altitude, and in placentas from pregnancies complicated by pre-eclampsia.
“It is increased in normal placentas from pregnancies at high altitude (121), and in placentas from pregnancies complicated by pre-eclampsia (272, 351, 401, 611).”
From Placental Origins of Chronic Disease (2016) · cites this paper as [351]
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Research works printed but not linked: 2
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Placental Origins of Chronic Disease
Burton, G. J., Fowden, A. L., & Thornburg, K. L. (2016) · Physiological Reviews · doi:10.1152/physrev.00029.2015 · Cited by 717 · see citing works
- Authors
- Luo, R., Wang, Y., Xu, P., Cao, G., Zhao, Y., Shao, X., Li, Y.-X., Chang, C., Peng, C., & Wang, Y.-L.
- Affiliations
- Chinese Academy of Sciences (CN)
- Shanxi University (CN)
- Peking University Third Hospital (CN)
- Lanzhou University (CN)
- York University (CA)
- ORCID
- Venue
- Scientific Reports
- Published
- 2016-01-22
- Volume
- 6
- Issue
- 1
- Pages
- 19588-19588
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4726282
- OpenAlex
- W2407164529
- Cited by
- 100
- References
- 58
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 3 · 2023: 9 · 2022: 9 · 2021: 14 · 2020: 12 · 2019: 15 · 2018: 12 · 2017: 13 · 2016: 6
- FWCI
- 19.77
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Lanzhou University Institutional Repository (submittedVersion)
- Funders
- Queen's University
- Topics
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Keywords
- preeclampsia, miR-210, THSD7A, hypoxia, placental trophoblast, microRNA target regulation, cell invasion, HTR-8/SVneo cells, placenta
- MeSH terms
- Adult, Hypoxia, Cell Movement, Female, Humans, Placenta, Pre-Eclampsia, Pregnancy, Trophoblasts, Reproducibility of Results, Down-Regulation, Cell Hypoxia, Thrombospondins, MicroRNAs
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