Castration influences intestinal microflora and induces abdominal obesity in high-fat diet-fed mice
Open accessAbstract
Late-onset hypogonadism (i.e. androgen deficiency) raises the risk for abdominal obesity in men. The mechanism for this obesity is unclear. Here, we demonstrated that hypogonadism after castration caused abdominal obesity in high-fat diet (HFD)-fed, but not in standard diet (SD)-fed, C57BL/6J mice. Furthermore, the phenotype was not induced in mice treated with antibiotics that disrupt the intestinal microflora. In HFD-fed mice, castration increased feed efficiency and decreased fecal weight per food intake. Castration also induced in an increase of visceral fat mass only in the absence of antibiotics in HFD-fed mice, whereas subcutaneous fat mass was increased by castration irrespective of antibiotics. Castration reduced the expression in the mesenteric fat of both adipose triglyceride lipase and hormone-sensitive lipase in HFD-fed mice, which was not observed in the presence of antibiotics. Castration decreased thigh muscle (i.e. quadriceps and hamstrings) mass, elevated fasting blood glucose levels and increased liver triglyceride levels in a HFD-dependent manner, whereas these changes were not observed in castrated mice treated with antibiotics. The Firmicutes/Bacteroidetes ratio and Lactobacillus species increased in the feces of HFD-fed castrated mice. These results show that androgen (e.g. testosterone) deficiency can alter the intestinal microbiome and induce abdominal obesity in a diet-dependent manner.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In mice, the microbiota can regulate testicular development and function, and androgen deficiency substantially alters the microbiome.
“In mice, the microbiota can regulate testicular development and function [251], while androgen deficiency has substantially altered the microbiome [252].”
From International Society of Sports Nutrition Position Stand: Probiotics (2019) · cites this paper as [252]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
1 retracted
Million, M., Maraninchi, M., Henry, M., Armougom, F., Richet, H., Carrieri, P., Valéro, R., Raccah, D., Vialettes, B., & Raoult, D. (2011) · International Journal of Obesity · doi:10.1038/ijo.2011.153 · Cited by 660 · see citing works
RetractedA SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189 · see citing works
Alberti, K. G. M. M., Eckel, R. H., Grundy, S. M., Zimmet, P. Z., Cleeman, J. I., Donato, K. A., Fruchart, J.-C., James, W. P. T., Loria, C. M., & Smith, S. C. (2009) · Circulation · doi:10.1161/circulationaha.109.192644 · Cited by 14,860 · see citing works
An obesity-associated gut microbiome with increased capacity for energy harvest
Turnbaugh, P. J., Ley, R. E., Mahowald, M. A., Magrini, V., Mardis, E. R., & Gordon, J. I. (2006) · Nature · doi:10.1038/nature05414 · Cited by 12,444 · see citing works
Human gut microbes associated with obesity
Ley, R. E., Turnbaugh, P. J., Klein, S., & Gordon, J. I. (2006) · Nature · doi:10.1038/4441022a · Cited by 8,785 · see citing works
Abdominal obesity and metabolic syndrome
Després, J.-P., & Lemieux, I. (2006) · Nature · doi:10.1038/nature05488 · Cited by 4,354 · see citing works
The Effect of Diet on the Human Gut Microbiome: A Metagenomic Analysis in Humanized Gnotobiotic Mice
Turnbaugh, P. J., Ridaura, V. K., Faith, J. J., Rey, F. E., Knight, R., & Gordon, J. I. (2009) · Science Translational Medicine · doi:10.1126/scitranslmed.3000322 · Cited by 3,058 · see citing works
Subcutaneous and visceral adipose tissue: Structural and functional differences
Ibrahim, M. M. (2010) · Obesity Reviews · doi:10.1111/j.1467-789x.2009.00623.x · Cited by 2,238 · see citing works
Sex differences in the gut microbiome drive hormone-dependent regulation of autoimmunity
Markle, J. G. M., Frank, D. N., Mortin-Toth, S., Robertson, C. E., Feazel, L. M., Rolle-Kampczyk, U., von Bergen, M., McCoy, K. D., Macpherson, A. J., & Danska, J. S. (2013) · Science · doi:10.1126/science.1233521 · Cited by 2,025 · see citing works
Diabetes and cardiovascular disease during androgen deprivation therapy for prostate cancer
Keating, N. L., O'Malley, A. J., & Smith, M. R. (2006) · Journal of Clinical Oncology · doi:10.1200/jco.2006.06.2497 · Cited by 1,455 · see citing works
Rose, C., Parker, A., Jefferson, B., & Cartmell, E. (2015) · Critical Reviews in Environmental Science and Technology · doi:10.1080/10643389.2014.1000761 · Cited by 1,248 · see citing works
The Decline of Androgen Levels in Elderly Men and Its Clinical and Therapeutic Implications
Kaufman, J. M., & Vermeulen, A. (2005) · Endocrine Reviews · doi:10.1210/er.2004-0013 · Cited by 1,079 · see citing works
Winzell, M. S., & Åhrén, B. (2004) · Diabetes · doi:10.2337/diabetes.53.suppl_3.s215 · Cited by 1,028 · see citing works
Low Serum Testosterone and Mortality in Older Men
Laughlin, G. A., Barrett-Connor, E., & Bergstrom, J. (2008) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2007-1792 · Cited by 798 · see citing works
Khaw, K.-T., Dowsett, M., Folkerd, E., Bingham, S., Wareham, N., Luben, R., Welch, A., & Day, N. (2007) · Circulation · doi:10.1161/circulationaha.107.719005 · Cited by 743 · see citing works
Changes in body composition during androgen deprivation therapy for prostate cancer
Smith, M. R., Finkelstein, J. S., McGovern, F. J., Zietman, A. L., Fallon, M. A., Schoenfeld, D. A., & Kantoff, P. W. (2002) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.87.2.8299 · Cited by 697 · see citing works
Surwit, R. S., Feinglos, M. N., Rodin, J., Sutherland, A., Petro, A. E., Opara, E. C., Kuhn, C. M., & Rebuffe-Scrive, M. (1995) · Metabolism · doi:10.1016/0026-0495(95)90123-x · Cited by 618 · see citing works
Animal models of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis
Takahashi, Y. (2012) · World Journal of Gastroenterology · doi:10.3748/wjg.v18.i19.2300 · Cited by 554 · see citing works
Gonadal effects on food intake and adiposity: a metabolic hypothesis.
Wade, G., & Gray, J. (1979) · Physiology and Behavior · doi:10.1016/0031-9384(79)90028-3 · Cited by 458 · see citing works
Hines, M., Brook, C. G. D., & Conway, G. S. (2004) · The Journal of Sex Research · doi:10.1080/00224490409552215 · Cited by 437 · see citing works
Million, M., Angelakis, E., Paul, M., Armougom, F., Leibovici, L., & Raoult, D. (2012) · Microbial Pathogenesis · doi:10.1016/j.micpath.2012.05.007 · Cited by 431 · see citing works
Interaction Between Obesity and the Gut Microbiota: Relevance in Nutrition
Delzenne, N. M., & Cani, P. D. (2011) · Annual Review of Nutrition · doi:10.1146/annurev-nutr-072610-145146 · Cited by 409 · see citing works
Metabolic Complications of Androgen Deprivation Therapy for Prostate Cancer
Saylor, P. J., & Smith, M. R. (2009) · The Journal of Urology · doi:10.1016/j.juro.2009.01.047 · Cited by 349 · see citing works
Androgens and body fat distribution
Blouin, K., Boivin, A., & Tchernof, A. (2007) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2007.09.001 · Cited by 306 · see citing works
The role of androgens in metabolism, obesity, and diabetes in males and females
Navarro, G., Allard, C., Xu, W., & Mauvais‐Jarvis, F. (2015) · Obesity · doi:10.1002/oby.21033 · Cited by 289 · see citing works
Fan, W., Yanase, T., Nomura, M., Okabe, T., Goto, K., Sato, T., Kawano, H., Kato, S., & Nawata, H. (2005) · Diabetes · doi:10.2337/diabetes.54.4.1000 · Cited by 269 · see citing works
Changes in fat and lean body mass during androgen-deprivation therapy for prostate cancer
Smith, M. R. (2004) · Urology · doi:10.1016/j.urology.2003.10.063 · Cited by 259 · see citing works
Host genetic and environmental effects on mouse intestinal microbiota
Campbell, J. H., Foster, C. M., Vishnivetskaya, T. A., Campbell, A. G., Yang, Z. K., Wymore, A. M., Palumbo, A. V., Chesler, E. J., & Podar, M. (2012) · The ISME Journal · doi:10.1038/ismej.2012.54 · Cited by 216 · see citing works
Increased hepatic steatosis and insulin resistance in mice lacking hepatic androgen receptor
Lin, H., Yu, I.-C. I., Wang, R.-S., Chen, Y., Liu, N.-C., Altuwaijri, S., Hsu, C., Ma, W., Jokinen, J. D., Sparks, J. D., et al. (2008) · Hepatology · doi:10.1002/hep.22252 · Cited by 210 · see citing works
MacLean, H. E., Chiu, W. S. M., Notini, A. J., Axell, A.-M., Davey, R. A., McManus, J. F., Ma, C., Plant, D. R., Lynch, G. S., & Zajac, J. D. (2008) · The FASEB Journal · doi:10.1096/fj.08-105726 · Cited by 209 · see citing works
Traish, A. M., Miner, M. M., Morgentaler, A., & Zitzmann, M. (2011) · The American Journal of Medicine · doi:10.1016/j.amjmed.2010.12.027 · Cited by 206 · see citing works
Late onset of obesity in male androgen receptor-deficient (AR KO) mice
Sato, T., Μatsumoto, T., Yamada, T., Watanabe, T., Kawano, H., & Kato, S. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)02774-2 · Cited by 196 · see citing works
Thigh circumference and risk of heart disease and premature death: prospective cohort study
Heitmann, B. L., & Frederiksen, P. (2009) · BMJ · doi:10.1136/bmj.b3292 · Cited by 186 · see citing works
Mårin, P., Lönn, L., Andersson, B., Odén, B., Olbe, L., Bengtsson, B. A., & Björntorp, P. (1996) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.81.3.8772568 · Cited by 172 · see citing works
Insulin and Leptin Resistance With Hyperleptinemia in Mice Lacking Androgen Receptor
Lin, H., Xu, Q., Yeh, S., Wang, R.-S., Sparks, J. D., & Chang, C. (2005) · Diabetes · doi:10.2337/diabetes.54.6.1717 · Cited by 172 · see citing works
Callewaert, F., Venken, K., Ophoff, J., Gendt, K. D., Torcasio, A., van Lenthe, H., Oosterwyck, H. V., Boonen, S., Bouillon, R., Verhoeven, G., et al. (2008) · The FASEB Journal · doi:10.1096/fj.08-113456 · Cited by 142 · see citing works
Androgenic control of food intake and body weight in male rats.
Rt, G., & Gn, W. (1976) · Journal of Comparative and Physiological Psychology · doi:10.1037/h0077264 · Cited by 136 · see citing works
Ophoff, J., Proeyen, K. V., Callewaert, F., Gendt, K. D., Bock, K. D., Bosch, A. V., Verhoeven, G., Hespel, P., & Vanderschueren, D. (2009) · Endocrinology · doi:10.1210/en.2008-1509 · Cited by 123 · see citing works
Krotkiewski, M., Kral, J. G., & Karlsson, J. (1980) · Acta Physiologica Scandinavica · doi:10.1111/j.1748-1716.1980.tb06592.x · Cited by 111 · see citing works
Role of the gut in visceral fat inflammation and metabolic disorders
Lam, Y. Y., Mitchell, A. J., Holmes, A. J., Denyer, G. S., Gummesson, A., Caterson, I. D., Hunt, N. H., & Storlien, L. H. (2011) · Obesity · doi:10.1038/oby.2011.68 · Cited by 97 · see citing works
Impact of testosterone on body fat composition
Maddalena, C. D., Vodo, S., Petroni, A., & Aloisi, A. M. (2012) · Journal of Cellular Physiology · doi:10.1002/jcp.24096 · Cited by 89 · see citing works
Human androgen deficiency: insights gained from androgen receptor knockout mouse models
Zajac, J. D., Rana, K., & Davey, R. A. (2014) · Asian Journal of Andrology · doi:10.4103/1008-682x.122590 · Cited by 84 · see citing works
Rana, K., Fam, B. C., Clarke, M. V., Pang, T. P. S., Zajac, J. D., & MacLean, H. E. (2011) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00584.2010 · Cited by 78 · see citing works
Androgen receptor knockout and knock-in mouse models
Kerkhofs, S., Denayer, S., Haelens, A., & Claessens, F. (2008) · Journal of Molecular Endocrinology · doi:10.1677/jme-08-0122 · Cited by 75 · see citing works
Androgen receptors and their biology
Lamb, D. J., Weigel, N. L., & Marcell, M. (2001) · Vitamins and hormones · doi:10.1016/s0083-6729(01)62005-3 · Cited by 69 · see citing works
Expression of androgen receptor target genes in skeletal muscle
Rana, K., Lee, N. K. L., Zajac, J. D., & MacLean, H. E. (2014) · Asian Journal of Andrology · doi:10.4103/1008-682x.122861 · Cited by 59 · see citing works
High fat diet induces central obesity, insulin resistance and microvascular dysfunction in hamsters
Costa, R. R. S., Villela, N. R., de Souza, M. D. G. C., Boa, B. C. S., Cyrino, F. Z. G. A., Silva, S. V., Lisboa, P. C., de Moura, E. G., Barja‐Fidalgo, T. C., & Bouskela, E. (2011) · Microvascular Research · doi:10.1016/j.mvr.2011.08.007 · Cited by 47 · see citing works
Christoffersen, B. Ø., Raun, K., Svendsen, O., Fledelius, C., & Golozoubova, V. (2006) · International Journal of Obesity · doi:10.1038/sj.ijo.0803261 · Cited by 41 · see citing works
Effects of castration on insulin levels and glucose tolerance in the mouse differ from those in man
Inoue, T., Zakikhani, M., David, S., Algire, C., Blouin, M., & Pollak, M. (2010) · The Prostate · doi:10.1002/pros.21198 · Cited by 27 · see citing works
Sex steroids effects on the endocrine pancreas
Morimoto, S., Morales, A., Zambrano, E., & Fernández‐Mejía, C. (2010) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2010.05.003 · Cited by 19 · see citing works
Cui, Y., Ma, J., Shi, Y., Huan, Q., Guo, H., Zhao, Y. Y., & Chen, Z. (2011) · Hormone and Metabolic Research · doi:10.1055/s-0031-1291366 · Cited by 2 · see citing works
Flagged first, then by citations
1 of the 52 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
The inflammatory microenvironment and microbiome in prostate cancer development
Sfanos, K. S., Yegnasubramanian, S., Nelson, W. G., & Marzo, A. M. D. (2017) · Nature Reviews Urology · doi:10.1038/nrurol.2017.167 · Cited by 470 · see citing works
Serum level of sex steroid hormone is associated with diversity and profiles of human gut microbiome
Shin, J.-H., Park, Y.-H., Sim, M., Kim, S.-A., Joung, H., & Shin, D.-M. (2019) · Research in Microbiology · doi:10.1016/j.resmic.2019.03.003 · Cited by 334 · see citing works
International Society of Sports Nutrition Position Stand: Probiotics
Jäger, R., Mohr, A. E., Carpenter, K. C., Kerksick, C. M., Purpura, M., Moussa, A., Townsend, J. R., Lamprecht, M., West, N. P., Black, K., et al. (2019) · Journal of the International Society of Sports Nutrition · doi:10.1186/s12970-019-0329-0 · Cited by 277 · see citing works
Sex, microbes, and polycystic ovary syndrome
Thackray, V. G. (2019) · Trends in Endocrinology & Metabolism · doi:10.1016/j.tem.2018.11.001 · Cited by 203 · see citing works
The microbiome in prostate inflammation and prostate cancer
Porter, C. M., Shrestha, E., Peiffer, L. B., & Sfanos, K. S. (2018) · Prostate Cancer and Prostatic Diseases · doi:10.1038/s41391-018-0041-1 · Cited by 207 · see citing works
Sex- and Gender-Based Pharmacological Response to Drugs
Mauvais‐Jarvis, F., Berthold, H. K., Campesi, I., Carrero, J. J., Dhakal, S., Franconi, F., Gouni‐Berthold, I., Heiman, M. L., Kautzky‐Willer, A., Klein, S. L., et al. (2021) · Pharmacological Reviews · doi:10.1124/pharmrev.120.000206 · Cited by 194 · see citing works
Thomas, M., Webb, M. J., Ghimire, S., Blair, A. D., Olson, K. C., Fenske, G. J., Fonder, A. T., Christopher‐Hennings, J., Brake, D. W., & Scaria, J. (2017) · Scientific Reports · doi:10.1038/s41598-017-12481-6 · Cited by 171 · see citing works
Microbiome and PCOS: State-of-Art and Future Aspects
Giampaolino, P., Foreste, V., Filippo, C. D., Gallo, A., Mercorio, A., Serafino, P., Improda, F. P., Verrazzo, P., Zara, G., Buonfantino, C., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22042048 · Cited by 169 · see citing works
Varghese, S., Rao, S., Khattak, A., Zamir, F., & Chaari, A. (2024) · Nutrients · doi:10.3390/nu16213663 · Cited by 152 · see citing works
Sfanos, K. S., Markowski, M. C., Peiffer, L. B., Ernst, S. E., White, J. R., Pienta, K. J., Antonarakis, E. S., & Ross, A. E. (2018) · Prostate Cancer and Prostatic Diseases · doi:10.1038/s41391-018-0061-x · Cited by 147 · see citing works
Diagnosis and management of secondary causes of steatohepatitis
Liebe, R., Esposito, I., Bock, H. H., Dahl, S. V., Stindt, J., Baumann, U., Luedde, T., & Keitel, V. (2021) · Journal of Hepatology · doi:10.1016/j.jhep.2021.01.045 · Cited by 108 · see citing works
Microbiome and Prostate Cancer: A Novel Target for Prevention and Treatment
Kuštrimović, N. Z., Bombelli, R., Baci, D., & Mortara, L. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24021511 · Cited by 98 · see citing works
Sex hormones influence the intestinal microbiota composition in mice
Wu, Y., Peng, X., Li, X., Li, D., Tan, Z., & Yu, R. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.964847 · Cited by 92 · see citing works
Androgens and the Regulation of Adiposity and Body Fat Distribution in Humans
Tchernof, A., Brochu, D., Maltais‐Payette, I., Mansour, M. F., Marchand, G. B., Carreau, A., & Kapeluto, J. (2018) · Comprehensive physiology · doi:10.1002/cphy.c170009 · Cited by 110 · see citing works
Sexual dimorphism of gut microbiota at different pubertal status
Yuan, X., Chen, R., Zhang, Y., Lin, X., & Yang, X. (2020) · Microbial Cell Factories · doi:10.1186/s12934-020-01412-2 · Cited by 86 · see citing works
The Interplay between Androgen and Gut Microbiota: Is There a Microbiota-Gut-Testis Axis
Li, X., Cheng, W., Shang, H., Wei, H., & Deng, C. (2021) · Reproductive Sciences · doi:10.1007/s43032-021-00624-0 · Cited by 84 · see citing works
Oseni, S. O., Naar, C., Pavlović, M. D., Asghar, W., Hartmann, J. X., Fields, G. B., Esiobu, N., & Kumi-Diaka, J. K. (2023) · Cancers · doi:10.3390/cancers15123110 · Cited by 94 · see citing works
Aguilera, J., Gálvez-Ontiveros, Y., & Rivas, A. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.578007 · Cited by 70 · see citing works
Sex-related Differences in Inflammatory Bowel Diseases: The Potential Role of Sex Hormones
Xu, L., Huang, G., Cong, Y., Yu, Y., & Li, Y. (2022) · Inflammatory Bowel Diseases · doi:10.1093/ibd/izac094 · Cited by 72 · see citing works
Hypogonadism alters cecal and fecal microbiota in male mice
Harada, N., Hanaoka, R., Hanada, K., Izawa, T., Inui, H., & Yamaji, R. (2016) · Gut Microbes · doi:10.1080/19490976.2016.1239680 · Cited by 68 · see citing works
Self-citationThe Microbiome and Prostate Cancer Risk
Wheeler, K. M., & Liss, M. A. (2019) · Current Urology Reports · doi:10.1007/s11934-019-0922-4 · Cited by 62 · see citing works
Estrogens and Body Weight Regulation in Men
Rubinow, K. B. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-70178-3_14 · Cited by 60 · see citing works
Chai, Z., Yan, Y.-H., Zan, S., Meng, X., & Zhang, F. (2022) · Food Research International · doi:10.1016/j.foodres.2022.111396 · Cited by 58 · see citing works
The Human Microbiota and Prostate Cancer: Friend or Foe?
Massari, F., Mollica, V., Nunno, V. D., Gatto, L., Santoni, M., Scarpelli, M., Cimadamore, A., Antonio Lopez-beltran, B., Liang Cheng, C., Battelli, N., et al. (2019) · Cancers · doi:10.3390/cancers11040459 · Cited by 57 · see citing works
Sex, puberty, and the gut microbiome
Sisk-Hackworth, L., Kelley, S. T., & Thackray, V. G. (2022) · Reproduction · doi:10.1530/rep-22-0303 · Cited by 51 · see citing works
Mukherjee, A. G., Wanjari, U. R., Kannampuzha, S., Murali, R., Namachivayam, A., Ganesan, R., Dey, A., Babu, A., Renu, K., Vellingiri, B., et al. (2023) · Metabolites · doi:10.3390/metabo13010129 · Cited by 52 · see citing works
Pérez-Matute, P., Íñiguez, M., de Toro, M., Recio-Fernández, E., & Oteo, J. A. (2020) · Scientific Reports · doi:10.1038/s41598-020-64961-x · Cited by 42 · see citing works
Human microbiome and prostate cancer development: current insights into the prevention and treatment
Moghadam, S. O., & Momeni, S. A. (2020) · Frontiers of Medicine · doi:10.1007/s11684-019-0731-7 · Cited by 41 · see citing works
Marchesin, J. D. C., Celiberto, L. S., Orlando, A. B., de Medeiros, A. I., Pinto, R. A., Zuanon, J. A. S., Spolidório, L. C., Santos, A., Taranto, M. P., & Cavallini, D. (2018) · Journal of Functional Foods · doi:10.1016/j.jff.2018.07.010 · Cited by 39 · see citing works
Apelo, S. I. A., Lin, A., Brinkman, J. A., Meyer, E. J., Morrison, M., Tomasiewicz, J. L., Pumper, C. P., Baar, E. L., Richardson, N. E., Alotaibi, M., et al. (2020) · eLife · doi:10.7554/elife.56177 · Cited by 37 · see citing works
Harada, N. (2018) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2018.1490172 · Cited by 36 · see citing works
Self-citationHou, X., Zhu, L., Zhang, X., Zhang, L., Bao, H., Tang, M.-M., Wei, R., & Wang, R. (2019) · Chemosphere · doi:10.1016/j.chemosphere.2019.01.101 · Cited by 36 · see citing works
Metabolomic effects of androgen deprivation therapy treatment for prostate cancer
Chi, J., Lin, P., Tolstikov, V., Oyekunle, T. O., Chen, E. Y.-S., Bussberg, V., Greenwood, B. P., Sarangarajan, R., Narain, N. R., Kiebish, M. A., et al. (2020) · Cancer Medicine · doi:10.1002/cam4.3016 · Cited by 36 · see citing works
Gut Microbiota and Sex Hormones: Crosstalking Players in Cardiometabolic and Cardiovascular Disease
Maffei, S., Forini, F., Canale, P., Nicolini, G., & Guiducci, L. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23137154 · Cited by 36 · see citing works
Song, T., Guan, X., Wang, X., Qu, S., Zhang, S., Hui, W., Men, L., & Chen, X. (2021) · The FASEB Journal · doi:10.1096/fj.201903129rrr · Cited by 34 · see citing works
Li, K.-M., Wang, L., Wong, C. Y., Chiu, P. K., Teoh, J. Y., Kwok, H. S., Leung, S. C. H., Wong, S. H., Tsui, S. K., & Ng, C. (2021) · Prostate Cancer and Prostatic Diseases · doi:10.1038/s41391-021-00360-1 · Cited by 33 · see citing works
Babu, A., Rajeswari, V. D., Ganesh, V., Das, S., Sivaraman, D., Rani, G. U., & Ramanathan, G. (2024) · Reproductive Sciences · doi:10.1007/s43032-023-01450-2 · Cited by 31 · see citing works
Male castration increases adiposity via small intestinal microbial alterations
Whon, T. W., Kim, H. S., Shin, N., Jung, E. S., Tak, E. J., Sung, H., Jung, M., Jeong, Y., Hyun, D., Kim, P. S., et al. (2020) · EMBO Reports · doi:10.15252/embr.202050663 · Cited by 31 · see citing works
Role of Biological Sex in the Cardiovascular-Gut Microbiome Axis
Li, S., & Kararigas, G. (2022) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2021.759735 · Cited by 29 · see citing works
Androgens and the Regulation of Adiposity and Body Fat Distribution in Humans
Tchernof, A., Brochu, D., Maltais‐Payette, I., Mansour, M. F., Marchand, G. B., Carreau, A., & Kapeluto, J. (2018) · Comprehensive physiology · doi:10.1002/j.2040-4603.2018.tb00043.x · Cited by 27 · see citing works
Perturbation on gut microbiota impedes the onset of obesity in high fat diet-induced mice
Yu, Z., Yu, X.-F., Kerem, G., & Ren, P. (2022) · Frontiers in Endocrinology · doi:10.3389/fendo.2022.795371 · Cited by 27 · see citing works
Agaro-Oligosaccharides Regulate Gut Microbiota and Adipose Tissue Accumulation in Mice
Higashimura, Y., Baba, Y., Inoüe, R., Takagi, T., Mizushima, K., Ohnogi, H., Honda, A., Matsuzaki, Y., & Naito, Y. (2017) · Journal of Nutritional Science and Vitaminology · doi:10.3177/jnsv.63.269 · Cited by 25 · see citing works
Hermoso, D. A. M., Bizerra, P. F. V., Constantin, R. P., Ishii‐Iwamoto, E. L., & Gilglioni, E. H. (2020) · The Aging Male · doi:10.1080/13685538.2020.1764927 · Cited by 24 · see citing works
Tang, L., Xie, P., Wang, H., Hong, X., Gong, Z., Zhao, G., & Yue, M. (2025) · Clinical Microbiology Reviews · doi:10.1128/cmr.00236-25 · Cited by 23 · see citing works
New Biomarkers to Evaluate Hyperandrogenemic Women and Hypogonadal Men
Karakas, S. E., & Surampudi, P. N. (2018) · Advances in clinical chemistry · doi:10.1016/bs.acc.2018.06.001 · Cited by 22 · see citing works
Venkatesh, V. S., Grossmann, M., Zajac, J. D., & Davey, R. A. (2022) · Obesity Reviews · doi:10.1111/obr.13429 · Cited by 22 · see citing works
Effect of Sheng‐Jiang Powder on Gut Microbiota in High‐Fat Diet‐Induced NAFLD
Li, J., Hu, Q., Dai, X.-Y., Zhu, L., Miao, Y., Kang, H., Zhao, X., Yao, J., Long, D., Tang, W., et al. (2020) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2020/6697638 · Cited by 21 · see citing works
Harada, N., Hanada, K., Minami, Y., Kitakaze, T., Ogata, Y., Tokumoto, H., Sato, T., Kato, S., Inui, H., & Yamaji, R. (2020) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00461.2019 · Cited by 19 · see citing works
Self-citationHarada, N., Minami, Y., Hanada, K., Hanaoka, R., Kobayashi, Y., Izawa, T., Sato, T., Kato, S., Inui, H., & Yamaji, R. (2020) · Gut Microbes · doi:10.1080/19490976.2020.1817719 · Cited by 19 · see citing works
Self-citationSex Differences in Intestinal Microbial Composition and Function of Hainan Special Wild Boar
Wang, X., Zhang, Y., Wen, Q., Wang, Y., Wang, Z., Tan, Z., & Wu, K. (2020) · Animals · doi:10.3390/ani10091553 · Cited by 18 · see citing works
Lin, M., Sun, X. K., & Lv, L. (2023) · Molecular Therapy — Oncolytics · doi:10.1016/j.omto.2023.04.007 · Cited by 18 · see citing works
Organski, A. C., Jorgensen, J. S., & Cross, T.-W. (2021) · Current Opinion in Endocrine and Metabolic Research · doi:10.1016/j.coemr.2021.100284 · Cited by 16 · see citing works
Metabolic and affective consequences of fatherhood in male California mice
Zhao, M., Garland, T., Chappell, M. A., Andrew, J. R., & Wendy Saltzman, A. (2017) · Physiology & Behavior · doi:10.1016/j.physbeh.2017.04.010 · Cited by 15 · see citing works
Newton, R. U., Christophersen, C. T., Fairman, C. M., Hart, N. H., Taaffe, D. R., Broadhurst, D., Devine, A., Chee, R., Tang, C., Spry, N. A., et al. (2019) · BMJ Open · doi:10.1136/bmjopen-2018-024872 · Cited by 16 · see citing works
Tanaka, C., Harada, N., Teraoka, Y., Urushizaki, H., Shinmori, Y., Onishi, T., Yotsumoto, Y., Ito, Y., Kitakaze, T., Inui, T., et al. (2024) · Scientific Reports · doi:10.1038/s41598-024-53380-x · Cited by 15 · see citing works
Self-citationTestosterone deficiency promotes arterial stiffening independent of sex chromosome complement
Sakamuri, A., Visniauskas, B., Kilanowski‐Doroh, I., McNally, A. B., Sugi, A. I., Kamau, A., Sengottaian, D., McLachlan, J. A., Anguera, M. C., Mauvais‐Jarvis, F., et al. (2024) · Biology of Sex Differences · doi:10.1186/s13293-024-00624-0 · Cited by 14 · see citing works
Zhang, W., Xie, J., Wang, Z., Zhong, Y., Liu, L., Liu, J., Zhang, W., Pi, Y., Tang, F., Liu, Z., et al. (2025) · Microbiological Research · doi:10.1016/j.micres.2025.128047 · Cited by 14 · see citing works
The Effect of Long-Term Hypogonadism on Body Composition and Morphometry of Aged Male Wistar Rats
Borbélyová, V., Šarayová, V., Renczés, E., Čonka, J., Janko, J., Šebeková, K., Stefikova, K., Ostatníková, D., & Celec, P. (2021) · Physiological Research · doi:10.33549/physiolres.934836 · Cited by 12 · see citing works
Fuzhuan brick tea supplemented with areca nuts: Effects on serum and gut microbiota in mice
Zhou, M., Tian, X., Wu, Z., Li, K., & Li, Z. (2021) · Journal of Food Biochemistry · doi:10.1111/jfbc.13737 · Cited by 12 · see citing works
Sisk-Hackworth, L., Brown, J., Sau, L., Levine, A. A., Tam, L. Y. I., Ramesh, A., Shah, R. S., Kelley-Thackray, E. T., Wang, S., Nguyen, A., et al. (2023) · Biology of Sex Differences · doi:10.1186/s13293-023-00564-1 · Cited by 13 · see citing works
Chevalley, T., Dübi, M., Fumeaux, L., Merli, M. S., Sarre, A., Schaer, N., Siméoni, U., & Yzydorczyk, C. (2025) · Cells · doi:10.3390/cells14060467 · Cited by 11 · see citing works
Fan, Y., Ren, Y., Deng, L., Lv, D., Chen, J., Ling, Y., Tu, J., Xu, X., Wang, D., & Cai, Z. (2024) · International Immunopharmacology · doi:10.1016/j.intimp.2024.113641 · Cited by 11 · see citing works
Does long-term androgen deficiency lead to metabolic syndrome in middle-aged rats?
Borbélyová, V., Renczés, E., Bábíčková, J., Tóthová, Ľ., Kačmárová, M., Uličná, O., Ostatníková, D., Hodosy, J., & Celec, P. (2017) · Experimental Gerontology · doi:10.1016/j.exger.2017.08.016 · Cited by 11 · see citing works
Different gut microbiome composition in obese Guizhou minipigs between female and castrated male
Yao, G., Wu, S., Zeng, X., Zhao, H., Wang, G., Chen, M., & Ning, Q. (2019) · Folia Microbiologica · doi:10.1007/s12223-019-00704-4 · Cited by 9 · see citing works
Castration induced circRNA expressional changes in subcutaneous adipose tissue of male pigs
Wang, J., Yang, Y., Xing, B., Chen, J., Lu, Q., Zhang, J., Ren, Q., Ma, Q., Guo, H., & Cao, H. X. (2021) · Animal Science Journal · doi:10.1111/asj.13648 · Cited by 9 · see citing works
Zhang, X., Song, T., Liu, G., Wu, J., Zhaxi, Y., Mustafa, S. B., Shahzad, K., Chen, X., Zhao, W., & Jiang, X. (2024) · Animals · doi:10.3390/ani14202942 · Cited by 8 · see citing works
Zhang, L., Cai, Y., Wei, S., Ling, Y., Zhu, L., Li, D., & Cai, Z. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17122125 · Cited by 8 · see citing works
Harada, N., Yotsumoto, Y., Katsuki, T., Yoda, Y., Masuda, T., Nomura, M., Shiraki, N., Inui, H., & Yamaji, R. (2019) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00173.2019 · Cited by 8 · see citing works
Self-citationReview on the induction of obesity in laboratory animals
Aswar, U. M., Patil, R., & Bodhankar, S. L. (2019) · Diabesity · doi:10.15562/diabesity.2019.58 · Cited by 7 · see citing works
Cui, J., Chen, H., Dong, F., Zheng, Q., Wu, J., Feng, B., Guo, D., Jiang, S., Li, M., & Li, J. (2025) · Discover Oncology · doi:10.1007/s12672-025-03296-3 · Cited by 6 · see citing works
Xiong, X., Xu, H., Wang, S., Liao, X., Yi, X., Jin, K., Lei, H., Bai, S., Qiu, S., & Yang, L. (2022) · Frontiers in Medicine · doi:10.3389/fmed.2021.800823 · Cited by 6 · see citing works
Castration reshapes the liver by altering fatty acid composition and metabolism in male mice
Yao, H., Li, D., Cao, X., Han, X., He, J., Cheng, D., Shang, J., Song, T., & Zeng, X. (2024) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2024.150319 · Cited by 5 · see citing works
Gut Microbiota Composition across Normal Range Prostate-Specific Antigen Levels
Kim, H., Kim, J. H., Chang, Y., Yang, D., Kim, H., & Ryu, S. (2021) · Journal of Personalized Medicine · doi:10.3390/jpm11121381 · Cited by 5 · see citing works
Cannizzaro, R., Maiero, S., Pelizzo, P., Gulotta, M., Facchin, S., Tessarolo, G., Zucchetto, A., Matrone, F., Realdon, S., & Bortolus, R. (2025) · Journal of Clinical Medicine · doi:10.3390/jcm14134783 · Cited by 4 · see citing works
Combined Effects of Exercise and Phytoanabolic Extracts in Castrated Male and Female Mice
Martins, J. P., Silva, L. C., Nunes, M. S., Rübensam, G., de Oliveira, J. R., Silva, R. B. M., & Campos, M. M. (2021) · Nutrients · doi:10.3390/nu13041177 · Cited by 4 · see citing works
Deminami, M., Hashimoto, M., Takahashi, H., Harada, N., Minami, Y., Kitakaze, T., Masuda, W., Takenaka, S., Inui, H., & Yamaji, R. (2024) · Bioscience Biotechnology and Biochemistry · doi:10.1093/bbb/zbae175 · Cited by 4 · see citing works
Self-citationSevere Visceral Obesity, Fatty Liver and Diabetes after Orchiectomy for Prostate Cancer
Kajitani, N., Takahashi, J., Honda, H., Hamahara, J., & Ando, S. (2020) · Internal Medicine · doi:10.2169/internalmedicine.4653-20 · Cited by 4 · see citing works
Nakamura, Y., Yoshida, M., Tanigawa, K., Harada, A., Kihara‐Negishi, F., Maruyama, K., & Karasawa, K. (2021) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.b20-00936 · Cited by 4 · see citing works
The Impact of Genetics on Gut Microbiota of Growing and Fattening Pigs under Moderate N Restriction
Sarri, L., Costa-Roura, S., Balcells, J., Seradj, A. R., & de la Fuente, G. (2021) · Animals · doi:10.3390/ani11102846 · Cited by 4 · see citing works
Inguinal Hernia in Nonhuman Primates: From Asymptomatic to Life-Threatening Events
de la Garza, M. A., Hegge, S. R., & Bakker, J. (2022) · Veterinary Sciences · doi:10.3390/vetsci9060280 · Cited by 4 · see citing works
Dorfman, M. D., Monfeuga, T., Melhorn, S. J., Kanter, J. E., Frey, J. M., Fasnacht, R. D., Chandran, A., Lala, E., Velasco, I., Rubinow, K. B., et al. (2023) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00059.2023 · Cited by 3 · see citing works
Gut Microbiota and Body Weight – A Review
Duca, I., & Dumitrescu, D. L. (2018) · Psihologijske teme · doi:10.31820/pt.27.1.3 · Cited by 3 · see citing works
Albuquerque, A., Garrido, N., Charneca, R., Egas, C., Martin, L., Ramos, A., Costa, F. J., Marmelo, C., & Martins, J. M. (2023) · Veterinary Sciences · doi:10.3390/vetsci10110641 · Cited by 3 · see citing works
The interplay of microbiota and hormone regulation in men with prostate cancer
Markowski, M. C., & Sfanos, K. S. (2021) · Prostate Cancer and Prostatic Diseases · doi:10.1038/s41391-021-00385-6 · Cited by 3 · see citing works
Yamada, Y., Urakawa, N., Tamiya, H., Sakamoto, S., Takahashi, H., Harada, N., Kitakaze, T., Izawa, T., Matsumura, S., Yoshihara, E., et al. (2025) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2025.110271 · Cited by 2 · see citing works
Self-citationTakahashi, H., Harada, N., Hayamizu, Y., Dungubat, E., Nakazawa, M., Kitakaze, T., Sugimoto, K., INUI, H., YOSHIHARA, E., Takahashi, Y., et al. (2026) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00518.2025 · Cited by 2 · see citing works
Self-citationEvaluation of the subcapsular technique for primary closure castration in donkeys (Equus asinus)
Ibrahim, A., Mahmoud, U. T., Ali, M. M., & Ragab, S. M. M. (2021) · Scientific Reports · doi:10.1038/s41598-021-93585-y · Cited by 2 · see citing works
Polycystic ovarian syndrome and microbiome: Implications for women’s health
Patel, N., Singh, D., Lokary, V., & Shaker, I. A. (2023) · Journal of Medical and Scientific Research · doi:10.17727/jmsr.2023/11-10 · Cited by 2 · see citing works
Yao, H., Li, D., Ye, S., Song, T., & Zeng, X. (2025) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2025.152233 · Cited by 2 · see citing works
Толстова, А. С., Морозов, М. В., Родин, О. В., Фазилов, Н. А., Кох, А. А., & Безжонова, А. А. (2021) · Естественные и Технические Науки · doi:10.37882/2223-2966.2021.05.29 · Cited by 2 · see citing works
The Gut and Urinary Microbiota: A Rising Biomarker in Genitourinary Malignancies
Pederzoli, F., Murdica, V., Salonia, A., & Alfano, M. (2022) · Springer eBooks · doi:10.1007/978-3-030-80546-3_19 · Cited by 2 · see citing works
Gut Microbiome: A Potential Controller of Androgen- Modulated Disease
Yu, Q. G. C. (2021) · Online Journal of Complementary & Alternative Medicine · doi:10.33552/ojcam.2021.06.000628 · Cited by 3 · see citing works
Никитина, Е. А., Орлова, С. В., Podoprigora, I. V., Грибакин, С. Г., Volkova, L. Y., Pigareva, Y. A., & Sergueïevna, D. M. (2022) · Medical alphabet · doi:10.33667/2078-5631-2022-16-29-39 · Cited by 1 · see citing works
Sun, Z., Huang, R., Chen, J., Song, Y., Sun, Z., Zhang, L., Shi, H., Mu, R., Wang, Y., Huang, J., et al. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1684165 · Cited by 1 · see citing works
Wang, Y., He, Z., Chen, J., Yang, M., & Zhang, L. (2025) · Global Ecology and Conservation · doi:10.1016/j.gecco.2025.e03969 · Cited by 1 · see citing works
Immunological, vascular and metabolic actions of androgens
Rodriguez, M. L. (2020)
Harada, N., & Yamaji, R. (2017) · KAGAKU TO SEIBUTSU · doi:10.1271/kagakutoseibutsu.56.2
Self-citationYan, Q., Chen, Y., Zhang, H., Liu, W., & Liu, J. (2025) · Medicine · doi:10.1097/md.0000000000044580
Additional file 1 of Sexual dimorphism of gut microbiota at different pubertal status
Yuan, X., Chen, R., Zhang, Y., Lin, X., & Yang, X. (2020) · Figshare · doi:10.6084/m9.figshare.12731394
Sakamuri, A., Visniauskas, B., Kilanowski‐Doroh, I., McNally, A. B., Sugi, A. I., Kamau, A. N., Sengottaian, D., McLachlan, J. A., Anguera, M. C., Mauvais‐Jarvis, F., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26730379
- Authors
- Harada, N., Hanaoka, R., Horiuchi, H., Kitakaze, T., Mitani, T., Inui, H., & Yamaji, R.
- Affiliations
- Osaka Prefecture University (JP)
- Shinshu University (JP)
- Corresponding author
- Harada, Naoki
- Venue
- Scientific Reports
- Published
- 2016-03-10
- Volume
- 6
- Issue
- 1
- Pages
- 23001-23001
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4785334
- OpenAlex
- W2296155309
- Cited by
- 110
- References
- 52
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 13 · 2023: 8 · 2022: 12 · 2021: 18 · 2020: 15 · 2019: 9 · 2018: 9 · 2017: 7 · 2016: 2
- FWCI
- 4.68
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (25450176)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- castration, androgen deficiency, hypogonadism, abdominal obesity, high-fat diet, gut microbiota, Firmicutes/Bacteroidetes ratio, Lactobacillus, visceral fat, subcutaneous adipose tissue, adipose triglyceride lipase, hormone-sensitive lipase, muscle mass, fasting blood glucose, hepatic triglycerides, antibiotic therapy, C57BL/6J mice
- MeSH terms
- Gastrointestinal Microbiome, Acyltransferases, Adipose Tissue, Androgens, Animals, Anti-Bacterial Agents, Blood Glucose, Castration, Humans, Hypogonadism, Lipase, Male, Gene Expression Regulation, Enzymologic, Mice, Obesity, Abdominal, Diet, High-Fat
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review