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Cited by 41Ginseng Biological Effects and ApplicationsSkin Protection and AgingGenomics, phytochemicals, and oxidative stress

Ginsenoside Rg1 attenuates ultraviolet B-induced glucocortisides resistance in keratinocytes via Nrf2/HDAC2 signalling

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Li, Jun, Liu, Dong, Wu, Jinfeng, Zhang, Daniel, Cheng, Binbin, Zhang, Yani, Yin, Zifei, Wang, Yuan +2 more·2016·Scientific Reports
DOI 10.1038/srep39336PMC PMC5159887
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Abstract

Oxidative stress, which occurs after ultraviolet (UV) radiation, usually results in Glucocorticoid (GC) resistance and the subsequent development of skin inflammation. One approach to protecting the skin against UV radiation is the use of antioxidants. The ginsenoside Rg1 is a novel natural antioxidant isolated from the medicinal plant Panax ginseng C.A. Mey. We demonstrated that UVB exposure exacerbated inflammation and reduced both the level of the glucocorticoid receptor (GR) and the efficacy of dexamethasone (Dex) in human keratinocytes (HaCaT cells). Pretreatment with Rg1 increased the expression of GR and restored Dex responsiveness to inflammation in UVB-irradiated HaCaT cells. Mechanistically, Rg1 rescued UVB-induced HDAC2 degradation. HDAC2 knockdown partially abolished the Rg1-induced up-regulation of GR and the enhancement of GC sensitivity. In addition, Rg1 reduced the production of reactive oxygen species (ROS), which preceded the up-regulation of HDAC2, and consequent sensitization of cells to Dex. Moreover, Rg1 treatment promoted the translocation and activation of Nrf2. Nrf2 knockdown partially abolished the Rg1-induced decrease of ROS production and increase of HDAC2. Rg1 also potentiated the anti-inflammatory effects of Dex in UVB-irradiated mouse skin. In conclusion, we demonstrated that Rg1 attenuated UVB-induced GC insensitivity. Notably, these effects were partially mediated by the Nrf2/HDAC2 pathway.

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  • Ginsenoside Rg2 (1) and ginsenoside Rg1 (4) have exhibited anti-aging effects in Hs68 and HaCaT cells, respectively, related to a reduction in MMP-1 expression and ROS.

    “In previous studies, ginsenoside Rg2 (1) and ginsenoside Rg1 (4) have exhibited anti-aging effects in Hs68 and HaCaT cells, respectively. These were related to a reduction in MMP-1 expression and ROS [17,31].”

    From Anti-Aging and Anti-Inflammatory Effects of Compounds from Fresh Panax ginseng Roots: A Study on TNF-α/IFN-γ-Induced Skin Cell Damage (2024) · cites this paper as [31]

    The Results section, “Rg1 restored HDAC2 levels through ROS inhibition” (page unavailable), reports that Rg1 reduced UVB-induced ROS production in HaCaT keratinocytes. The paper investigates glucocorticoid resistance and skin inflammation, not an anti-aging effect in those cells; it does not report reduced MMP-1 expression. The Rg2/Hs68 finding is not attributed specifically to this source in the multiply cited sentence. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Li, J., Liu, D., Wu, J., Zhang, D., Cheng, B., Zhang, Y., Yin, Z., Wang, Y., Du, J., & Ling, C.
Affiliations
  • Second Military Medical University (CN)
  • Fudan University (CN)
  • University of Florida Health (US)
Corresponding author
  • Du, Juan
  • Ling, Changquan
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2016-12-16
Volume
6
Issue
1
Pages
39336-39336
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/srep39336
PMC
PMC5159887
OpenAlex
W2562433766
Cited by
41
References
7
Citations by year
2026: 4 · 2025: 5 · 2024: 4 · 2023: 3 · 2022: 7 · 2021: 2 · 2020: 9 · 2019: 6 · 2017: 1
FWCI
1.21
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Shanghai Municipal Education Commission (E03008)
  • E-Institutes of Shanghai Municipal Education Commission (E 03008, No E 03008)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ginseng Biological Effects and Applications
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
Keywords
ginsenoside Rg1, UV-B radiation, glucocorticoid resistance, keratinocytes, Nrf2, HDAC2, glucocorticoid receptor, dexamethasone, oxidative stress, reactive oxygen species, skin inflammation, Panax ginseng, anti-inflammatory activity
MeSH terms
Antioxidants, Cells, Cultured, Dexamethasone, Glucocorticoids, Humans, Ultraviolet Rays, Signal Transduction, Keratinocytes, Reactive Oxygen Species, Oxidative Stress, Ginsenosides, NF-E2-Related Factor 2, Histone Deacetylase 2

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