Formation of 2′-deoxyuridine hydrates upon exposure of nucleosides to gamma radiation and UVC-irradiation of isolated and cellular DNA
Abstract
The two diastereoisomers of 6-hydroxy-5,6-dihydro-2'-deoxyuridine (dUrd hydrates) are produced upon deamination of the related 2'-deoxycytidine derivatives. Liquid chromatography coupled to tandem mass spectrometry was used to quantify dUrd hydrates. Their rate constant of formation within UVC-irradiated solutions of dCyd was first determined. Their formation was also shown to occur within solutions of either dUrd or dCyd exposed to gamma-rays in the absence of oxygen. dUrd hydrates were then quantified within isolated and cellular DNA exposed to UVC light. In both cases, their yield of formation was found to be, at least, 2 orders of magnitude lower than the overall yield of bipyrimidine photoproducts such as cyclobutane dimers and (6-4) photoproducts.
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Cytosine hydrates and adenine-containing dimers have been isolated in model systems, but their yields are about two orders of magnitude lower than bipyrimidine photoproducts in double-stranded DNA.
“Other photoproducts such as cytosine hydrates (41,42) or adenine-containing dimers (43,44) have been isolated in model systems but their yields are 2 orders of magnitude lower than that of bipyrimidine photoproducts in double-stranded (ds) DNA.”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [42]
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- Authors
- Douki, T., Vadesne-Bauer, G., & Cadet, J.
- Affiliations
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- Corresponding author
- Douki, Thierry
- Cadet, Jean
- Venue
- Photochemical & Photobiological Sciences
- Published
- 2002-06-13
- Volume
- 1
- Issue
- 8
- Pages
- 565-569
- ISSN
- 1474-905X (ISSN-L)
- 1474-9092
- OpenAlex
- W1977709378
- Cited by
- 22
- References
- 27
- Citations by year
- 2024: 1 · 2022: 1 · 2019: 1 · 2014: 5 · 2013: 3 · 2012: 2
- FWCI
- 0.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Photochemical & Photobiological Sciences (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- diastereomers, 2'-deoxycytidine, deamination, gamma radiation, UV-C irradiation, DNA damage, cyclobutane dimers, 6-4 photoproducts, radiation chemistry, DNA adducts
- MeSH terms
- Animals, Cattle, Chromatography, High Pressure Liquid, Deoxyuridine, DNA, Gamma Rays, Nucleosides, Mass Spectrometry, Ultraviolet Rays, Water
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