The variety of UV-induced pyrimidine dimeric photoproducts in DNA as shown by chromatographic quantification methods
Abstract
Induction of DNA damage is one of the major consequences of exposure to solar UV radiation in living organisms. UV-induced DNA photoproducts are mostly pyrimidine dimers, including cyclobutane pyrimidine dimers, pyrimidine (6-4) pyrimidone photoproducts and Dewar valence isomers. In the last few decades, a large number of methods have been developed for the quantification of these pyrimidine dimers. The present review emphasizes the contribution of chromatographic techniques to our better understanding of the basic DNA photochemistry and the better description of damage in cells.
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Nucleosomes play a pivotal role in protecting T-T rich sequences from UV light.
“Nucleosomes play the pivotal role in protecting those T-T rich sequences from UV light [1,16].”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [16]
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Toxicity and DNA repair in normal human keratinocytes co-exposed to benzo[a]pyrene and sunlight
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Blue light impairs the repair of UVB‐induced pyrimidine dimers in a human skin model
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Madsen, M. M., Jones, N. C., Nielsen, S. B., & Hoffmann, S. V. (2016) · Physical Chemistry Chemical Physics · doi:10.1039/c6cp05980e · Cited by 13 · see citing works
DNA damage mediated by UV radiation and relative repair mechanisms in mammals
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The Potential of Spirulina and Its Bioactive Metabolites as Ingested Agents for Skin Care
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Huo, H., He, Y., Chen, W., Wu, L., Yi, X., & Wang, J. (2021) · Talanta · doi:10.1016/j.talanta.2021.123081 · Cited by 13 · see citing works
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DNA photoreacts by nucleobase ring cleavage to form labile isocyanates
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Giussani, A., & Worth, G. A. (2020) · The Journal of Physical Chemistry Letters · doi:10.1021/acs.jpclett.0c01264 · Cited by 10 · see citing works
Bezabih, M. S., Kaliakin, D. S., Blanco-González, A., Barneschi, L., Tarnovsky, A. N., & Olivucci, M. (2023) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.3c06433 · Cited by 9 · see citing works
Nicholson, W. L., Schuerger, A. C., & Douki, T. (2018) · Astrobiology · doi:10.1089/ast.2017.1721 · Cited by 9 · see citing works
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Hidalgo, D., & Martín-Marroquín, J. M. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-815259-1.00001-x · Cited by 9 · see citing works
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Zannier, F., Portero, L. R., Douki, T., Gärtner, W., Farı́as, M. E., & Albarracín, V. H. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.791714 · Cited by 7 · see citing works
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Benabou, S., Ruckebusch, C., Sliwa, M., Aviñó, A. M., Eritja, R., Gargallo, R., & de Juan, A. (2018) · Physical Chemistry Chemical Physics · doi:10.1039/c8cp00850g · Cited by 6 · see citing works
Multiscale Conformational Sampling Reveals Excited-State Locality in DNA Self-Repair Mechanism
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N4‐Methylation of Cytosine Drastically Favors the Formation of (6‐4) Photoproducts in a TCG Context
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Generation and characterization of CRISPR-Cas9-mediated XPC gene knockout in human skin cells
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Schalk, O., & Tapavicza, E. (2020) · ACS in focus · doi:10.1021/acs.infocus.7e4009 · Cited by 5 · see citing works
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Distinguishing Isomeric Cyclobutane Thymidine Dimers by Ion Mobility and Tandem Mass Spectrometry
Yang, H.-C., Scruggs, S. S., Chai, M., George, M., Taylor, J., & Gross, M. L. (2024) · Journal of the American Society for Mass Spectrometry · doi:10.1021/jasms.4c00133 · Cited by 3 · see citing works
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Self-citationFAM122A maintains DNA stability possibly through the regulation of topoisomerase IIα expression
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The remarkable UV light invulnerability of thymine GNA dinucleotides
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Faisal, A., Lerche, C. M., Douki, T., Philipsen, P. A., Pihl, C., Gregersen, T., Granborg, J. R., BJERRING, P., Hædersdal, M., & Wiegell, S. R. (2026) · Photochemical & Photobiological Sciences · doi:10.1007/s43630-026-00884-2 · Cited by 2 · see citing works
Self-citationQuantification of DNA Damage Using Mass Spectrometry Techniques
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Self-citation
- Authors
- Douki, T.
- Affiliations
- Université Joseph Fourier (FR)
- Corresponding author
- Douki, Thierry
- ORCID
- Douki, Thierry
- Venue
- Photochemical & Photobiological Sciences
- Published
- 2013-08
- Volume
- 12
- Issue
- 8
- Pages
- 1286-1302
- ISSN
- 1474-905X (ISSN-L)
- 1474-9092
- OpenAlex
- W2032445218
- Cited by
- 132
- References
- 157
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 12 · 2023: 8 · 2022: 11 · 2021: 14 · 2020: 10 · 2019: 11 · 2018: 14 · 2017: 7 · 2016: 11 · 2015: 5 · 2014: 13 · 2013: 5
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Photochemical & Photobiological Sciences (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-10-BLAN-0809, ANR-12-BS08-0001)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- UV-induced DNA damage, pyrimidine dimers, cyclobutane pyrimidine dimers, chromatographic quantification, DNA photochemistry, solar ultraviolet radiation, chromatography
- MeSH terms
- Animals, Chromatography, DNA, DNA Damage, Humans, Pyrimidine Dimers, Ultraviolet Rays
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