DNA Base Damage by Reactive Oxygen Species, Oxidizing Agents, and UV Radiation
Open accessAbstract
Emphasis has been placed in this article dedicated to DNA damage on recent aspects of the formation and measurement of oxidatively generated damage in cellular DNA in order to provide a comprehensive and updated survey.This includes single pyrimidine and purine base lesions, intrastrand cross-links, purine 5 0 ,8-cyclonucleosides, DNA-protein adducts and interstrand cross-links formed by the reactions of either the nucleobases or the 2-deoxyribose moiety with the hydroxyl radical, one-electron oxidants, singlet oxygen, and hypochlorous acid.In addition, recent information concerning the mechanisms of formation, individual measurement, and repair-rate assessment of bipyrimidine photoproducts in isolated cells and human skin upon exposure to UVB radiation, UVA photons, or solar simulated light is critically reviewed.I n this article, we emphasize recent develop- ments in the formation of damage to cellular DNA mediated by reactive oxygen species (ROS) and oxidizing agents, including singlet oxygen, the hydroxyl radical ( † OH), one-electron oxidants, hypochlorous acid (HOCl), and ten-eleven translocation (TET) oxygenases involved in epigenetic regulation.These advances have been possible because of the development of sensitive and powerful high-performance liquid chromatography-mass spectrometry (HPLC-MS)/ mass spectrometry (MS) methods allowing one to revise previously reported data obtained using methods such as gas chromatographymass spectrometry (GC-MS), immunoassays, and HPLC with single MS detection (Cadet et al. 2011(Cadet et al. , 2012a)).Considerable progress has also been made in the elucidation of oxidative degradation pathways of isolated DNA and related model compounds (for recent comprehensive reviews, see Gimisis and Cismas ¸2006;Neeley and Essigmann 2006;Pratviel and Meunier 2006;von Sonntag 2006;Cadet et al. 2008Cadet et al. , 2010 Cadet et al. , 2012b;;Dedon 2008;Burrows 2009;Wagner and Cadet 2010).In addition, there is much complementary information on solar-radiation-induced formation of bipyrimidine photoproducts in the DNA of fibroblasts, keratinocytes, and human skin.In particular, the distribution of UVA and UVB photoproducts has been determined, allowing accurate determination of their rates of repair (Cadet et al. 2009(Cadet et al. , 2012c)).
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UV radiation damages DNA by forming various photoproducts, generating free radicals, and introducing strand breaks.
“Deteriorating effects of UV radiation on DNA include formation of various photoproducts, generation of free radicals and subsequent introduction of strand breaks [3].”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [3]
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Singlet oxygen induces oxidation of cellular DNA.
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Self-citationTautomeric equilibria in 8-oxopurines: Implications for mutagenicity
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Vicinal Lesions in X-irradiated DNA?
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Self-citationUV-Induced TA Photoproducts: Formation and Hydrolysis in Double-Stranded DNA
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Isolation, Characterization, and Independent Synthesis of Guanine Oxidation Products
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Sharifi-Rad, M., Anil Kumar, N. V., Zucca, P., Varoni, E. M., Dini, L., Panzarini, E., Rajkovic, J., Tsouh Fokou, P. V., Azzini, E., Peluso, I., et al. (2020) · Frontiers in Physiology · doi:10.3389/fphys.2020.00694 · Cited by 1,971 · see citing works
“Finally, ROS can damage DNA through oxidizing deoxyribose, breaking strand, removing nucleotides, modifying bases and crosslinking DNA-protein (Sharma et al., 2012; Cadet and Wagner, 2013; Cadet et al., 2017; Liang et al., 2020).”
BackgroundAbbas, G., Murtaza, B., Bibi, I., Shahid, M. A. R., Niazi, N. K., Khan, M. I., Amjad, M., Hussain, M., & Natasha, N. (2018) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph15010059 · Cited by 896 · see citing works
“Thus, the produced ROS can generate DNA–protein adducts and cause DNA and oxidative base damage [252], a break-up of chromatid/ chromosome or exchange [250], the formation of apyrimidinic/apurinic sites [253], DNA– protein cross-links [254], chromosomal aberrations, sister chromatid exchange, micronuclei formation, an…”
BackgroundRestoration of TET2 Function Blocks Aberrant Self-Renewal and Leukemia Progression
Cimmino, L., Dolgalev, I., Wang, Y., Yoshimi, A., Martin, G. H., Wang, J., Ng, V., Xia, B., Witkowski, M. T., Mitchell-Flack, M., et al. (2017) · Cell · doi:10.1016/j.cell.2017.07.032 · Cited by 707 · see citing works
Modulating ROS to overcome multidrug resistance in cancer
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Singlet Molecular Oxygen Reactions with Nucleic Acids, Lipids, and Proteins
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Self-citationFunctions of ROS in Macrophages and Antimicrobial Immunity
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“Excessive oxidative stress causes damage to cellular components such as proteins, DNA and lipids and results in a number of different pathologies [76–78].”
BackgroundInfluentialThe emerging role of RNA modifications in the regulation of mRNA stability
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Sleep Loss Can Cause Death through Accumulation of Reactive Oxygen Species in the Gut
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The impact of oxidative DNA damage and stress on telomere homeostasis
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Bactericidal Antibiotics Induce Toxic Metabolic Perturbations that Lead to Cellular Damage
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“Hydroxyl radicals react with biomolecules at diffusion-controlled rates in a reaction volume that is less than 2 nm in cells (Cadet and Wagner, 2013).”
BackgroundDNA origami nanostructures can exhibit preferential renal uptake and alleviate acute kidney injury
Jiang, D., Ge, Z., Im, H., England, C. G., Ni, D., Hou, J., Zhang, L., Kutyreff, C. J., Yan, Y., Liu, Y., et al. (2018) · Nature Biomedical Engineering · doi:10.1038/s41551-018-0317-8 · Cited by 449 · see citing works
Formation of UV-induced DNA damage contributing to skin cancer development
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Self-citationPhotodynamic Therapy: Current Status and Future Directions
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Hypermutation in human cancer genomes: footprints and mechanisms
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Flavonoids and their role in oxidative stress, inflammation, and human diseases
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“both purine and purine bases (28,30,133) whereas hydrogen abstraction within the sugar moiety leads in most cases to the formation of strand breaks (67,143–145).”
BackgroundSelf-citationFormation and repair of oxidatively generated damage in cellular DNA
Cadet, J., Davies, K. J. A., de Medeiros, M. H. G., Mascio, P. D., & Wagner, J. R. (2017) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2016.12.049 · Cited by 356 · see citing works
“• OH-mediated oxidation products of thymine (Thy) involved the cis and trans isomers of ThyGly, 5-hydroxymethylhydantoin (HydThy), 5-hydroxymethyluracil (5-HmUra) and 5formyluracil (5-FoUra) as previously characterized in model studies including isolated DNA [28,65,217].”
BackgroundSelf-citationBiocompatibility characteristics of the metal organic framework ZIF-8 for therapeutical applications
Hoop, M., Walde, C. F., Riccò, R., Mushtaq, F., Terzopoulou, A., Chen, X., deMello, A. J., Doonan, C. J., Falcaro, P., Nelson, B. J., et al. (2018) · Applied Materials Today · doi:10.1016/j.apmt.2017.12.014 · Cited by 350 · see citing works
“[51, 52] Augmented intracellular levels of ROS cause the arrest of the cell cycle at the G2/M checkpoint, a necessary step for DNA repair mechanism.[53] If ROS levels are above a certain threshold, DNA repair is no longer possible, and cellular apoptosis pathways are eventually induced.”
BackgroundReactive Oxygen Species and Antioxidants in Postharvest Vegetables and Fruits
Meitha, K., Pramesti, Y., & Suhandono, S. (2020) · International Journal of Food Science · doi:10.1155/2020/8817778 · Cited by 342 · see citing works
“In addition, changes in the nucleotides in one chain can cause incompatibility with nucleotides in other chains, eventually causing mutations [45].”
BackgroundTumor-Associated Neutrophils in Cancer: Going Pro
Wu, L., Saxena, S., Awaji, M. I., & Singh, R. K. (2019) · Cancers · doi:10.3390/cancers11040564 · Cited by 338 · see citing works
“However, the released ROS by neutrophils can result in DNA base damages [54], as well as mutations [55], which are essential for cancer initiation, cell proliferation, cancer-favored inflammation, immune suppression [56], and EMT in multiple cancer types, including breast cancer [57].”
BackgroundAhirwar, R., & Tripathi, A. K. (2020) · Environmental Nanotechnology Monitoring & Management · doi:10.1016/j.enmm.2020.100409 · Cited by 317 · see citing works
Update on Keratinocyte Carcinomas
Nehal, K. S., & Bichakjian, C. K. (2018) · New England Journal of Medicine · doi:10.1056/nejmra1708701 · Cited by 315 · see citing works
Silver nanoparticles in aquatic environments: Physiochemical behavior and antimicrobial mechanisms
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Reactive oxygen species: a volatile driver of field cancerization and metastasis
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Oxidatively induced DNA damage and its repair in cancer
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DNA Damage: From Threat to Treatment
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“Similarly, Reactive Oxygen Species (ROS) or Reactive Nitrogen Species (RNS) are generally produced as byproducts of multiple physiological activities in diverse subcellular sites [4].”
BackgroundRole of reactive oxygen species in ultraviolet-induced photodamage of the skin
Wei, M., He, X., Liu, N., & Deng, H. (2024) · Cell Division · doi:10.1186/s13008-024-00107-z · Cited by 244 · see citing works
“Moreover, ROS can affect the overexpression of p53 and p21 cip1/ waf1 , leading to the failure of DNA or apoptosis repair [49].”
BackgroundAchieving Controlled Biomolecule–Biomaterial Conjugation
Spicer, C. D., Pashuck, E. T., & Stevens, M. M. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00253 · Cited by 239 · see citing works
Base excision repair of oxidative DNA damage from mechanism to disease
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Exploiting innate immunity for cancer immunotherapy
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Neutrophil-induced genomic instability impedes resolution of inflammation and wound healing
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“As such, ROS can induce genotoxic stress by mediating DNA alkylation, generating abasic sites (9), and oxidizing DNA to promote formation of 8-oxoguanine (8oxoG), one of the most prevalent DNA lesions (10) removed by base excision repair (BER) (10).”
BackgroundThe current state of eukaryotic DNA base damage and repair
Bauer, N. C., Corbett, A. H., & Doetsch, P. W. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv1136 · Cited by 216 · see citing works
“Purine oxidative reaction mechanisms and products are reviewed in (12,18,19).”
BackgroundPao, P.-C., Patnaik, D., Watson, L. A., Gao, F., Pan, L., Wang, J., Adaikkan, C., Penney, J., Cam, H. P., Huang, W.-C., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-16361-y · Cited by 216 · see citing works
Impaired ribosome biogenesis: mechanisms and relevance to cancer and aging
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“Since ROS are potent inducers of DNA damage [133, 134], proficient expression of TCOF1 in neural crest cells is essential.”
BackgroundDual Role of Reactive Oxygen Species and their Application in Cancer Therapy
Huang, R., Chen, H., Liang, J., Li, Y., Yang, J., Luo, C., Tang, Y., Ding, Y., Liu, X., Yuan, Q., et al. (2021) · Journal of Cancer · doi:10.7150/jca.54699 · Cited by 218 · see citing works
“ROS also oxidize A with two main products: 2-hydroxy-2’deoxyadenosine (2-OH-dA) [36] and 7,8-dihydro-8oxo-2’-deoxyadenosine (8-oxo-dA) [32].”
BackgroundRecent Developments in Antimicrobial Polymers: A Review
Santos, M. R. E., Fonseca, A. C., Mendonça, P. V., Branco, R., Serra, A. C., Morais, P. V., & Coelho, J. F. J. (2016) · Materials · doi:10.3390/ma9070599 · Cited by 212 · see citing works
“In the case of DNA, the ROS species attacks the sugar group, pyrimidine and purine bases yielding single- and double-strand breaks, several adducts and cross-links to other molecules [44].”
BackgroundPhotosensitization Reactions of Biomolecules: Definition, Targets and Mechanisms
Baptista, M. S., Cadet, J., Greer, A., & Thomas, A. H. (2021) · Photochemistry and Photobiology · doi:10.1111/php.13470 · Cited by 205 · see citing works
“1 Hydration of pyrimidine S•+ gives rise to C-centered radicals (Scheme 3a) that efficiently react with O2 to produce peroxyl radicals; these transient species may be either reduced, likely by O2 •−, into related hydroperoxides or react selectively with vicinal bases/sugar moiety in DNA, thus forming intrastrand tande…”
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Antioxidant Activity, Metabolism, and Bioavailability of Polyphenols in the Diet of Animals
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Snake extract–laden hemostatic bioadhesive gel cross-linked by visible light
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How do cells cope with RNA damage and its consequences?
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“Of the 20 canonical amino acids, cysteine and methionine are most susceptible to ROS.”
BackgroundInfluentialNeutrophils in the Tumor Microenvironment
Wu, L., Saxena, S., & Singh, R. K. (2020) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-35723-8_1 · Cited by 186 · see citing works
RNA-binding Proteins in Regulating mRNA Stability and Translation: Roles and Mechanisms in Cancer
Li, W., Deng, X., & Chen, J.-J. (2022) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2022.03.025 · Cited by 168 · see citing works
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“19 oxidation of DNA bases by ROS which induces DNA breakage (Cadet and Wagner, 2013), iii) dsDNA breakage into ssDNA (Santos et al.”
BackgroundFocus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies
Kciuk, M., Marciniak, B., Mojzych, M., & Kontek, R. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21197264 · Cited by 168 · see citing works
“Deteriorating effects of UV radiation on DNA include formation of various photoproducts, generation of free radicals and subsequent introduction of strand breaks [3].”
BackgroundThe Many Facets of Therapy Resistance and Tumor Recurrence in Glioblastoma
Goenka, A., Tiek, D., Song, X., Huang, T., Hu, B., & Cheng, S.-Y. (2021) · Cells · doi:10.3390/cells10030484 · Cited by 169 · see citing works
“The conventional mechanism of action for radiation is dependent on reactive oxygen species (ROS) that cause DNA damage resulting in oxidative stress [89].”
BackgroundCadet, J., & Wagner, J. R. (2014) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2014.05.001 · Cited by 169 · see citing works
Self-citationNew insights into abasic site repair and tolerance
Thompson, P. S., & Cortez, D. (2020) · DNA repair · doi:10.1016/j.dnarep.2020.102866 · Cited by 170 · see citing works
“Reactive oxygen species such as superoxide, hydrogen peroxide, and hydroxyl radicals are by-products of cellular metabolism that can modify all four DNA bases [11].”
BackgroundTransport and accumulation of plasma generated species in aqueous solution
Verlackt, C. C. W., Boxem, W. V., & Bogaerts, A. (2018) · Physical Chemistry Chemical Physics · doi:10.1039/c7cp07593f · Cited by 166 · see citing works
Detection and interpretation of 8-oxodG and 8-oxoGua in urine, plasma and cerebrospinal fluid
Poulsen, H. E., Llovera, L., Broedbaek, K., Nielsen, P. E., & Weimann, A. (2013) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2013.06.009 · Cited by 162 · see citing works
Proanthocyanidins against Oxidative Stress: From Molecular Mechanisms to Clinical Applications
Yang, L., Xian, D., Xiong, X., Lai, R., Song, J., & Zhong, J. (2018) · BioMed Research International · doi:10.1155/2018/8584136 · Cited by 160 · see citing works
“Exposure to UV can induce the formation of highly mutagenic cyclobutane pyrimidine dimers (CPDs) and other free radicals potentially damaging to genomic DNA [60, 61].”
BackgroundCellular Stresses and Stress Responses in the Pathogenesis of Insulin Resistance
Onyango, A. N. (2018) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2018/4321714 · Cited by 160 · see citing works
“8-oxoG is most readily formed by singlet oxygen, although the hydroxyl radical also contributes to its formation [180, 181].”
BackgroundYun, B. H., Guo, J., Bellamri, M., & Turesky, R. J. (2018) · Mass Spectrometry Reviews · doi:10.1002/mas.21570 · Cited by 157 · see citing works
One-electron oxidation reactions of purine and pyrimidine bases in cellular DNA
Cadet, J., Wagner, J. R., Shafirovich, V., & Geacintov, N. E. (2013) · International Journal of Radiation Biology · doi:10.3109/09553002.2013.877176 · Cited by 152 · see citing works
“…jean.cadet@cea.fr (Received 13 November 2013 ; revised 10 December 2013 ; accepted 12 December 2013 ) induction to the nucleobases and the 2-deoxyribose moiety (von Sonntag 1987a, 2006, Dedon 2008, O ’ Neill and Wardman 2009, Cadet et al. 2010, Cadet and Wagner 2013, Georgakilas et al. 2013).”
BackgroundSelf-citationSimulation of early DNA damage after the irradiation of a fibroblast cell nucleus using Geant4-DNA
Meylan, S., Incerti, S., Karamitros, M., Tang, N., Bueno, M. T., Clairand, I., & Villagrasa, C. (2017) · Scientific Reports · doi:10.1038/s41598-017-11851-4 · Cited by 154 · see citing works
DNA damage related crosstalk between the nucleus and mitochondria
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“Thymine on the other hand is susceptible to •OH-mediated damage resulting in lesions such as 5,6dihydroxy-5,6-dihydrothymine [43].”
BackgroundPhotochemical and Photodynamical Properties of Sulfur‐Substituted Nucleic Acid Bases,
Ashwood, B., Pollum, M., & Crespo‐Hernández, C. E. (2018) · Photochemistry and Photobiology · doi:10.1111/php.12975 · Cited by 149 · see citing works
“Because 6-thio-20-deoxyguanosine has a lower oxidation potential than all of the nucleic acid bases (40), it is a target of oxidatively generated damage (33,34,41).”
BackgroundImmune System Modulation and Viral Persistence in Bats: Understanding Viral Spillover
Subudhi, S., Rapin, N., & Misra, V. (2019) · Viruses · doi:10.3390/v11020192 · Cited by 146 · see citing works
“Controlling excessive inflammation Unique antiviral response Persistent viral infection D ecreasing antibody levels Low ering of innate im m une response Stressful events A rousal from hibernation Secondary infection C onfinem ent in cages H abitat destruction Spillover Recrudescence of viral infection Dampened activa…”
BackgroundInfluentialThe immunomodulatory role of matrix metalloproteinases in colitis-associated cancer
He, L., Kang, Q., Chan, K. I., Zhang, Y., Zhong, Z., & Tan, W. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2022.1093990 · Cited by 144 · see citing works
Oxidation and alkylation stresses activate ribosome-quality control
Yan, L. L., Simms, C. L., McLoughlin, F., Vierstra, R. D., & Zaher, H. S. (2019) · Nature Communications · doi:10.1038/s41467-019-13579-3 · Cited by 123 · see citing works
Solar UV Radiation-Induced DNA Bipyrimidine Photoproducts: Formation and Mechanistic Insights
Cadet, J., Grand, A., & Douki, T. (2014) · Topics in current chemistry · doi:10.1007/128_2014_553 · Cited by 130 · see citing works
Self-citationNucleotide-Resolution Genome-Wide Mapping of Oxidative DNA Damage by Click-Code-Seq
Wu, J., McKeague, M., & Sturla, S. J. (2018) · Journal of the American Chemical Society · doi:10.1021/jacs.8b03715 · Cited by 123 · see citing works
Poly(ADP-ribose) polymerases covalently modify strand break termini in DNA fragmentsin vitro
Talhaoui, I., Lebedeva, N. A., Zarkovic, G., Saint‐Pierre, C., Kutuzov, M. M., Sukhanova, M. V., Matkarimov, B. T., Gasparutto, D., Saparbaev, M. K., Lavrik, O. I., et al. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw675 · Cited by 128 · see citing works
“Cellular DNA is constantly damaged by exogenous and endogenous factors resulting in base and sugar alterations and DNA strand breaks (1).”
Telomeric 8-oxo-guanine drives rapid premature senescence in the absence of telomere shortening
Barnes, R. P., Rosa, M. D., Thosar, S. A., Detwiler, A. C., Roginskaya, V. Y., Houten, B. V., Bruchez, M. P., Stewart-Ornstein, J., & Opresko, P. L. (2022) · Nature Structural & Molecular Biology · doi:10.1038/s41594-022-00790-y · Cited by 136 · see citing works
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Activation of the DNA damage response in vivo in synucleinopathy models of Parkinson’s disease
Milanese, C., Cerri, S., Ulusoy, A., Gornati, S. V., Plat, A., Gabriels, S., Blandini, F., Di Monte, D. A., Hoeijmakers, J. H., & Mastroberardino, P. G. (2018) · Cell Death & Disease · doi:10.1038/s41419-018-0848-7 · Cited by 118 · see citing works
Oxidative Stress in Cancer Therapy: Friend or Enemy?
Azmanova, M., & Pitto‐Barry, A. (2022) · ChemBioChem · doi:10.1002/cbic.202100641 · Cited by 119 · see citing works
Antibacterial activity of metal oxide nanoparticles
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The biology of tardigrade disordered proteins in extreme stress tolerance
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Research progress on skin photoaging and oxidative stress
Chen, X., Yang, C., & Jiang, G. (2021) · Advances in Dermatology and Allergology · doi:10.5114/ada.2021.112275 · Cited by 120 · see citing works
Oxidative Stress, Mitochondrial Dysfunction and the Mitochondria Theory of Aging
Kong, Y., Trabucco, S. E., & Zhang, H. (2014) · Interdisciplinary topics in gerontology and geriatrics · doi:10.1159/000358901 · Cited by 112 · see citing works
Strategies for the evaluation of DNA damage and repair mechanisms in cancer
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“DNA is the primary target following exposure to stimuli such as ultraviolet (UV) radiation, DNA alkylators, certain environmental carcinogens, oxidative stress and chemotherapeutic drugs (1).”
InfluentialEstrogen-related receptors are targetable ROS sensorsPreprint
Vernier, M., Dufour, C. R., McGuirk, S., Scholtes, C., Li, X., Bourmeau, G., Kuasne, H., Park, M., St‐Pierre, J., Audet‐Walsh, É., et al. (2020) · Genes & Development · doi:10.1101/gad.330746.119 · Cited by 110 · see citing works
“Excessive ROS can lead to the oxidative damage of macromolecules, in particular nucleic acids (Cadet and Wagner 2013); thus, we next evaluated ROS-induced DNA damage in response to modulation of ERRα and ERRγ activity.”
BackgroundZhou, H., He, Z., Cao, Y., Chu, L., Liang, B., Yu, K., & Deng, Z. (2024) · Theranostics · doi:10.7150/thno.97412 · Cited by 115 · see citing works
“In particular, the hydroxyl radical • OH, which has the strongest cytotoxicity, can react indiscriminately with nucleic acids, lipids and proteins, leading to an abnormal inflammatory storm and inhibiting the osteogenesis of bone marrow mesenchymal stem cells [11, 12].”
BackgroundMain nitric oxide (NO) hallmarks to relieve arsenic stress in higher plants
Bhat, J. A., Ahmad, P., & Corpas, F. J. (2021) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2020.124289 · Cited by 107 · see citing works
ATM: Expanding roles as a chief guardian of genome stability
Shiloh, Y. (2014) · Experimental Cell Research · doi:10.1016/j.yexcr.2014.09.002 · Cited by 107 · see citing works
Spampinato, C. P. (2016) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-016-2436-2 · Cited by 110 · see citing works
“metabolic processes that induce, either directly or indirectly, DNA damage [1].”
BackgroundLi, Y., Su, L., Zhang, Y., Liu, Y., Huang, F., Ren, Y., An, Y., Shi, L., van der Mei, H. C., & Busscher, H. J. (2022) · Advanced Science · doi:10.1002/advs.202103485 · Cited by 105 · see citing works
Radiotherapy Using High-Intensity Pulsed Radiation Beams (FLASH): A Radiation-Chemical Perspective
Wardman, P. (2020) · Radiation Research · doi:10.1667/rade-19-00016 · Cited by 104 · see citing works
“[Addition of a peroxyl radical to an adjacent base is also possible (70).”
BackgroundModel studies of the role of oxygen in the FLASH effect
Favaudon, V., Labarbe, R., & Limoli, C. L. (2021) · Medical Physics · doi:10.1002/mp.15129 · Cited by 101 · see citing works
Burke, C. S., Byrne, A., & Keyes, T. E. (2018) · Journal of the American Chemical Society · doi:10.1021/jacs.8b02711 · Cited by 100 · see citing works
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“Contact with such flavorant agents results in elevated level of ROS production and excessive IL-8 secretion in airway epithelium cells, with both mechanisms leading to cell cytotoxicity: oxidative stress is a cause of DNA damage [29].”
BackgroundOxidative Stress and Marine Carotenoids: Application by Using Nanoformulations
Genç, Y., Bardakçı, H., Yücel, Ç., Karatoprak, G. Ş., Akkol, E. K., Barak, T. H., & Sobarzo‐Sánchez, E. (2020) · Marine Drugs · doi:10.3390/md18080423 · Cited by 94 · see citing works
“The main target of oxidative damage in the DNA chain is purine, pyrimidine bases and sugar structure [47].”
BackgroundDNA damage as a biological sensor for environmental sunlight
Schuch, A. P., Machado Garcia, C. C., Makita, K., & Menck, C. F. M. (2013) · Photochemical & Photobiological Sciences · doi:10.1039/c3pp00004d · Cited by 90 · see citing works
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Next-generation DNA damage sequencing
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Excessive Reactive Oxygen Species and Exotic DNA Lesions as an Exploitable Liability
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Wang, J., Potocny, A. M., Rosenthal, J., & Day, E. S. (2019) · ACS Omega · doi:10.1021/acsomega.9b04150 · Cited by 89 · see citing works
Physical exercise and catecholamines response: benefits and health risk: possible mechanisms
Kruk, J., Kotarska, K., & Aboul-Enein, B. H. (2020) · Free Radical Research · doi:10.1080/10715762.2020.1726343 · Cited by 89 · see citing works
“The decomposition of H2O2 by the transition metal ions leads to HO● generation the most reactive oxygen species (reaction 6) which induces lipid peroxidation, structural alteration of DNA, increases DNA mutation and its damage [1,123,152,185,186].”
BackgroundMeasurement of oxidatively induced DNA damage and its repair, by mass spectrometric techniques
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Reactive Oxygen Species: Do They Play a Role in Adaptive Immunity?
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“Highly reactive hydroxyl radicals can directly interact with DNA leading to DNA damage (85).”
BackgroundYu, S., & Kim, S. J. (2014) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2014.2014 · Cited by 85 · see citing works
“ROS are destructive to DNA and proteins (11).”
The Effects of Ionising and Non-Ionising Electromagnetic Radiation on Extracellular Matrix Proteins
Tuieng, R. J., Cartmell, S. H., Kirwan, C., & Sherratt, M. J. (2021) · Cells · doi:10.3390/cells10113041 · Cited by 85 · see citing works
“The cellular effects of both (1)O2 and HO· are well studied [47,49,54].”
BackgroundOxidative Stress in Poultry: Lessons from the Viral Infections
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Zhou, B., Yang, Y., Pang, X., Shi, J., Jiang, T., & Zheng, X. (2023) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2023.115071 · Cited by 81 · see citing works
- Authors
- Cadet, J., & Wagner, J. R.
- Affiliations
- Université de Sherbrooke (CA)
- Venue
- Cold Spring Harbor Perspectives in Biology
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2013-02-01
- Volume
- 5
- Issue
- 2
- Pages
- a012559-a012559
- ISSN
- 1943-0264 (ISSN-L)
- PMC
- PMC3552502
- OpenAlex
- W2129637612
- Cited by
- 903
- References
- 139
- Citations by year
- 2026: 55 · 2025: 74 · 2024: 65 · 2023: 77 · 2022: 83 · 2021: 116 · 2020: 105 · 2019: 97 · 2018: 85 · 2017: 45 · 2016: 42 · 2015: 22 · 2014: 32 · 2013: 5
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cold Spring Harbor Perspectives in Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- DNA base damage, reactive oxygen species, hydroxyl radicals, singlet oxygen, hypochlorous acid, one-electron oxidants, UV-B radiation, UVA, simulated sunlight, DNA repair, interstrand cross-links, intrastrand cross-links, epigenetic regulation, HPLC-MS/MS quantification
- MeSH terms
- Chromatography, High Pressure Liquid, DNA Damage, Nucleotides, Oxidation-Reduction, Mass Spectrometry, Ultraviolet Rays, Reactive Oxygen Species, DNA Adducts
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