Disposable electrochemiluminescent biosensor for lactate determination in saliva
Abstract
An electrochemiluminescence-based disposable biosensor for lactate is characterized. The lactate recognition system is based on lactate oxidase (LOx) and the transduction system consists of luminol. All the needed reagents, luminol, LOx, BSA, electrolyte and buffer have been immobilized by a Methocel membrane placed on the working electrode of the screen-printed electrochemical cell. The measurement of the electrochemiluminescence (ECL) is made possible via a photocounting head when 50 microl of sample is placed into the screen-printed cell with a circular container containing the disposable sensing membrane. The compositions of the membrane and reaction conditions have been optimized to obtain adequate sensitivity. The disposable biosensor responds to lactate after 20 s when two 1 s pulses at 0.5 V are applied to obtain the analytical parameter, the ECL initial rate. The linearized double logarithmic dependence for lactate shows a dynamic range from 10(-5) to 5 x 10(-4) M with a detection limit of 5 x 10(-6) M and a sensor-to-sensor repeatability, as relative standard deviation, RSD, of 3.30% at the medium level of the range. The ECL disposable biosensor was applied to the analysis of lactate in human saliva as an alternative procedure for obtaining the lactate level in a non-invasive way. Interferences coming from components of saliva were studied and eliminated in a simple way that was easy to handle. The procedure was validated for use in human saliva, comparing the results against an enzymatic reference procedure. The proposed method is quick, inexpensive, selective and sensitive and uses conventional ECL instrumentation.
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Because saliva can be readily collected, it has been used in connection to in vitro diagnostic biosensors on strips or portable device platforms.
“Because saliva can be readily collected123-125, it has been used in connection to in vitro diagnostic biosensors on strips or portable device platforms,126-134.”
From Wearable biosensors for healthcare monitoring (2019) · cites this paper as [129]
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- Authors
- Ballesta Claver, J., Valencia Mirón, M. C., & Capitán-Vallvey, L. F.
- Affiliations
- Universidad de Granada (ES)
- Corresponding author
- Capitán-Vallvey, L. F.
- Venue
- The Analyst
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2009
- Volume
- 134
- Issue
- 7
- Pages
- 1423
- ISSN
- 0003-2654 (ISSN-L)
- 1364-5528
- OpenAlex
- W2149311683
- Cited by
- 93
- References
- 58
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 6 · 2023: 7 · 2022: 6 · 2021: 7 · 2020: 9 · 2019: 8 · 2018: 4 · 2017: 9 · 2016: 1 · 2015: 4 · 2014: 8 · 2013: 4 · 2012: 6
- FWCI
- 4.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Analyst (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Keywords
- electrochemiluminescence biosensor, disposable biosensor, lactate determination, saliva testing, lactate oxidase, luminol, screen-printed electrodes
- MeSH terms
- Methanol, Animals, Buffers, Cattle, Disposable Equipment, Electrochemistry, Electrodes, Electrolytes, Graphite, Humans, Hydrogen Peroxide, Hydrogen-Ion Concentration, Mixed Function Oxygenases, Indicators and Reagents, Lactates, Luminescent Measurements, Luminol, Membranes, Artificial, Oxidation-Reduction, Saliva, Serum Albumin, Bovine, Temperature, Biosensing Techniques
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