Cited by 93Electrochemical sensors and biosensorsAdvanced biosensing and bioanalysis techniquesElectrochemical Analysis and Applications

Disposable electrochemiluminescent biosensor for lactate determination in saliva

Ballesta Claver, J., Valencia Mirón, M. C., Capitán-Vallvey, L. F.·2009·The Analyst

Abstract

An electrochemiluminescence-based disposable biosensor for lactate is characterized. The lactate recognition system is based on lactate oxidase (LOx) and the transduction system consists of luminol. All the needed reagents, luminol, LOx, BSA, electrolyte and buffer have been immobilized by a Methocel membrane placed on the working electrode of the screen-printed electrochemical cell. The measurement of the electrochemiluminescence (ECL) is made possible via a photocounting head when 50 microl of sample is placed into the screen-printed cell with a circular container containing the disposable sensing membrane. The compositions of the membrane and reaction conditions have been optimized to obtain adequate sensitivity. The disposable biosensor responds to lactate after 20 s when two 1 s pulses at 0.5 V are applied to obtain the analytical parameter, the ECL initial rate. The linearized double logarithmic dependence for lactate shows a dynamic range from 10(-5) to 5 x 10(-4) M with a detection limit of 5 x 10(-6) M and a sensor-to-sensor repeatability, as relative standard deviation, RSD, of 3.30% at the medium level of the range. The ECL disposable biosensor was applied to the analysis of lactate in human saliva as an alternative procedure for obtaining the lactate level in a non-invasive way. Interferences coming from components of saliva were studied and eliminated in a simple way that was easy to handle. The procedure was validated for use in human saliva, comparing the results against an enzymatic reference procedure. The proposed method is quick, inexpensive, selective and sensitive and uses conventional ECL instrumentation.

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Registry

Crossref · 10.1039/b821922b

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58 of 58 references verified0 citation errors · 0 not checkable

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  • Because saliva can be readily collected, it has been used in connection to in vitro diagnostic biosensors on strips or portable device platforms.

    “Because saliva can be readily collected123-125, it has been used in connection to in vitro diagnostic biosensors on strips or portable device platforms,126-134.”

    From Wearable biosensors for healthcare monitoring (2019) · cites this paper as [129]

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Authors
Ballesta Claver, J., Valencia Mirón, M. C., & Capitán-Vallvey, L. F.
Affiliations
Universidad de Granada (ES)
Corresponding author
Capitán-Vallvey, L. F.
Venue
The Analyst
Published
2009
Volume
134
Issue
7
Pages
1423
ISSN
  • 0003-2654 (ISSN-L)
  • 1364-5528
OpenAlex
W2149311683
Cited by
93
References
58
Citations by year
2026: 3 · 2025: 1 · 2024: 6 · 2023: 7 · 2022: 6 · 2021: 7 · 2020: 9 · 2019: 8 · 2018: 4 · 2017: 9 · 2016: 1 · 2015: 4 · 2014: 8 · 2013: 4 · 2012: 6
FWCI
4.23
Type
journal article
Language
en
Open access
closed
Locations
  • The Analyst (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
  • Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
Keywords
electrochemiluminescence biosensor, disposable biosensor, lactate determination, saliva testing, lactate oxidase, luminol, screen-printed electrodes
MeSH terms
Methanol, Animals, Buffers, Cattle, Disposable Equipment, Electrochemistry, Electrodes, Electrolytes, Graphite, Humans, Hydrogen Peroxide, Hydrogen-Ion Concentration, Mixed Function Oxygenases, Indicators and Reagents, Lactates, Luminescent Measurements, Luminol, Membranes, Artificial, Oxidation-Reduction, Saliva, Serum Albumin, Bovine, Temperature, Biosensing Techniques

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