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Cited by 68Bone Tissue Engineering MaterialsPolymer Surface Interaction StudiesSurface Treatment and Coatings

Covalent immobilization of collagen on titanium through polydopamine coating to improve cellular performances of MC3T3-E1 cells

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Yu, Xiaohua, Walsh, John, Wei, Mei·2014·RSC Advances
DOI 10.1039/c3ra44137gPMC PMC4053246
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Abstract

Surface modification of orthopedic implants is critical for improving the clinical performance of these medical devices. Herein, collagen was covalently immobilized onto a titanium implant surface via a novel adherent polydopamine coating inspired by mussel adhesive proteins. The formation and composition of the collagen coating was characterized using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Fluorescent labeled collagen was also used to examine the formation and uniformity of the collagen coating. The resultant collagen coating with a polydopamine supporting substrate demonstrated better uniformity and distribution on the titanium surface compared to a physical adsorption of collagen. The covalent immobilized collagen coating is biologically active, as evidenced by its ability to enhance MC3T3-E1 cell adhesion, support cell proliferation and promote early stage osteogenic differentiation of pre-osteoblasts. Our study suggests covalent immobilization of collagen through the polydopamine coating might be an efficient way to improve the cellular performance of implant surfaces.

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  • A layer-by-layered composite bone biosubstitute combining VEGF, PDA layer, and Ti material significantly facilitates proliferation of endothelial cells.

    “Such a layer-by-layered composite bone biosubstitute combining VEGF, PDA layer, and Ti material significantly facilitates the attachment and proliferation of endothelial cells, as well as benefiting the differentiation of human mesenchymal stem cells (hMSC) to endothelial cells.”

    From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [89]

    The Results (§3.4; page unavailable) report increased proliferation of MC3T3-E1 pre-osteoblasts on treated titanium surfaces, including polydopamine-coated titanium. The paper studies collagen, not VEGF, and does not test endothelial-cell proliferation or hMSC differentiation on the claimed composite. Its Discussion mentions endothelial cells only when describing a separate study using dopamine-coated stainless steel. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

  • A layer-by-layered composite bone biosubstitute combining VEGF, PDA layer, and Ti material significantly facilitates attachment of endothelial cells.

    “Such a layer-by-layered composite bone biosubstitute combining VEGF, PDA layer, and Ti material significantly facilitates the attachment and proliferation of endothelial cells, as well as benefiting the differentiation of human mesenchymal stem cells (hMSC) to endothelial cells.”

    From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [89]

    The Results (section 3.3) report improved adhesion of MC3T3-E1 pre-osteoblast cells on collagen-coated, polydopamine-treated titanium. The paper does not report a VEGF-containing composite or test endothelial-cell attachment on titanium; its Discussion mentions endothelial cells only when describing another study using dopamine-coated stainless steel. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • A layer-by-layered composite bone biosubstitute combining VEGF, PDA layer, and Ti material benefits differentiation of hMSC to endothelial cells.

    “Such a layer-by-layered composite bone biosubstitute combining VEGF, PDA layer, and Ti material significantly facilitates the attachment and proliferation of endothelial cells, as well as benefiting the differentiation of human mesenchymal stem cells (hMSC) to endothelial cells.”

    From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [89]

    The Methods (section 2.4) describe titanium and polydopamine-coated substrates, and the Discussion reports improved adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 pre-osteoblasts. The paper does not report a VEGF-containing composite or differentiation of human mesenchymal stem cells into endothelial cells; its mention of endothelial-cell attachment and proliferation concerns a different study. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • PDA coating layer can effectively immobilize VEGF on a Ti-based biosubstitute without adverse effect on the activity of VEGF.

    “Furthermore, the PDA coating layer can effectively immobilize vascular endothelial growth factor (VEGF) on the Ti-based biosubstitute without the adverse effect on the activity of VEGF [89].”

    From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [89]

    The Conclusion (page unavailable) reports effective immobilization of type I collagen on titanium through a polydopamine coating. It does not report VEGF immobilization or test whether VEGF retains its activity, so the VEGF-specific claim is unsupported. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

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Authors
Yu, X., Walsh, J., & Wei, M.
Affiliations
University of Connecticut (US)
Corresponding author
  • Yu, Xiaohua
  • Wei, Mei
ORCID
Venue
RSC Advances
Publisher
Royal Society of Chemistry (RSC)
Published
2014
Volume
4
Issue
14
Pages
7185
ISSN
2046-2069 (ISSN-L)
DOI
10.1039/c3ra44137g
PMC
PMC4053246
OpenAlex
W2151107533
Cited by
68
References
43
Citations by year
2025: 3 · 2024: 2 · 2023: 3 · 2022: 8 · 2021: 5 · 2020: 4 · 2019: 10 · 2018: 9 · 2017: 7 · 2016: 8 · 2015: 6 · 2014: 3
FWCI
3.13
Type
journal article
Language
en
Open access
Locations
  • RSC Advances (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Science Foundation
  • University of Connecticut
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (R21AR059962)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
  • Physical Sciences › Engineering › Mechanical Engineering › Surface Treatment and Coatings
Keywords
collagen immobilization, polydopamine coating, titanium implants, orthopedic implants, surface modification, covalent immobilization, MC3T3-E1 cells, osteoblast adhesion, cell proliferation, osteogenic differentiation, bone tissue engineering

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